{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T14:56:23Z","timestamp":1768834583658,"version":"3.49.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","award":["AQ-16"],"award-info":[{"award-number":["AQ-16"]}],"id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology"],"published-print":{"date-parts":[[2017,3]]},"DOI":"10.1016\/j.cbpb.2016.11.010","type":"journal-article","created":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T02:45:12Z","timestamp":1480560312000},"page":"13-29","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"special_numbering":"C","title":["Molecular cloning, characterization and expression profile of kisspeptin1 and kisspeptin1 receptor at brain-pituitary-gonad (BPG) axis of golden mahseer, Tor putitora (Hamilton, 1822) during gonadal development"],"prefix":"10.1016","volume":"205","author":[{"given":"Neetu","family":"Shahi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atul Kumar","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monalisa","family":"Sahoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumanta Kumar","family":"Mallik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimpal","family":"Thakuria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.cbpb.2016.11.010_bb0005","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.ygcen.2013.03.030","article-title":"Expression of kisspeptins and their receptors, gnrh-1\/gnrh-II-1a and gonadotropin genes in the brain of adult male and female European sea bass during different gonadal stages","volume":"187","author":"Alvarado","year":"2013","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0010","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1095\/biolreprod.107.066266","article-title":"Molecular identification and functional characterization of the kisspeptin\/kisspeptin receptor system in lower vertebrates","volume":"79","author":"Biran","year":"2008","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0015","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1002\/cne.23211","article-title":"Expression of kiss1 and kiss2 genes in the brain of the European sea bass (Dicentrarchus labrax): relationships with estrogen receptors and pituitary gonadotrophs","volume":"52","author":"Escobar","year":"2013","journal-title":"J. Comp. Neurol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0020","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.mce.2008.11.017","article-title":"Evidence for two distinct KiSS genes in non-placental vertebrates that encodes kisspeptins with different gonadotropin-releasing activities in fish and mammals","volume":"312","author":"Felip","year":"2009","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0025","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1006\/frne.1999.0181","article-title":"Gonadotropin-releasing hormone genes: phylogeny, structure, and functions","volume":"20","author":"Fernald","year":"1999","journal-title":"Front. Neuroendocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0030","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1095\/biolreprod.107.063420","article-title":"The kisspeptin\/gonadotropin-releasing hormone pathway and molecular signalling of puberty in fish","volume":"78","author":"Filby","year":"2008","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0035","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1016\/j.bbrc.2003.11.066","article-title":"The KiSS-1 receptor GPR54 is essential for the development of the murine reproductive system","volume":"312","author":"Funes","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0040","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0022136","article-title":"Rise of the earliest tetrapods: an early Devonian origin from marine environment","volume":"6","author":"George","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0045","doi-asserted-by":"crossref","first-page":"11349","DOI":"10.1523\/JNEUROSCI.3328-05.2005","article-title":"Activation of gonadotropin-releasing hormone neurons by kisspeptin as a neuoendocrine switch for the onset of puberty","volume":"25","author":"Han","year":"2005","journal-title":"J. Neurosci."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0006-8993(99)01975-7","article-title":"Neuropeptide families and their receptors: evolutionary perspectives","volume":"848","author":"Hoyle","year":"1999","journal-title":"Brain Res."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0055","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1210\/en.2007-1503","article-title":"Identification of KiSS-1 product kisspeptin and steroid sensitivity sexually dimorphic kisspeptin neurons in medaka (Oryzias latipes)","volume":"149","author":"Kanda","year":"2008","journal-title":"Endocrinology"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0060","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/1471-2105-6-62","article-title":"A standard curve based method for relative real time PCR data processing","volume":"6","author":"Larionov","year":"2005","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0065","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0014-5793(99)00009-5","article-title":"Discovery of a receptor related to the galanin receptors","volume":"446","author":"Lee","year":"1999","journal-title":"FEBS Lett."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0070","doi-asserted-by":"crossref","first-page":"2837","DOI":"10.1210\/en.2008-1679","article-title":"Molecular evolution of multiple forms of kisspeptins and GPR54 receptors in vertebrates","volume":"150","author":"Lee","year":"2009","journal-title":"Endocrinology"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0075","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1677\/JOE-09-0016","article-title":"Structural and functional multiplicity of the kisspeptin\/GPR54 system in goldfish (Carassius auratus)","volume":"201","author":"Li","year":"2009","journal-title":"J. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0080","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1095\/biolreprod.108.072173","article-title":"Identification of two isoforms of the kisspeptin-1 receptor (kiss1r) generated by alternative splicing in a modern teleost, the Senegalese sole (Solea senegalensis)","volume":"80","author":"Mechaly","year":"2009","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0085","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.mce.2009.11.005","article-title":"Gene structure of the Kiss1 receptor-2 (Kiss1r-2) in the Atlantic halibut: insights into the evolution and regulation of Kiss1r genes","volume":"317","author":"Mechaly","year":"2010","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0090","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.mce.2011.03.004","article-title":"Gene structure analysis of kisspeptin-2 (Kiss2) in the Senegalese sole (Solea senegalensis): characterization of two splice variants of Kiss2, and novel evidence of metabolic regulation of kisspeptin signalling in non-mammalian species","volume":"339","author":"Mechaly","year":"2011","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0095","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.ygcen.2012.07.033","article-title":"Kisspeptin and seasonal control of reproduction in male European sea bass (Dicentrarchus labrax)","volume":"179","author":"Migaud","year":"2012","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0100","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/32937","article-title":"Rhodopsin-family receptors associate with small G proteins to activate phospholipase D","volume":"392","author":"Mitchell","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0105","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1677\/jme.1.02182","article-title":"Developmental expression of the G protein-coupled receptor 54 and three GnRH mRNAs in the teleost fish cobia","volume":"38","author":"Mohamed","year":"2007","journal-title":"J. Mol. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0110","doi-asserted-by":"crossref","first-page":"28969","DOI":"10.1074\/jbc.M102743200","article-title":"AXOR12, a novel human G protein-coupled receptor, activated by the peptide KiSS-1","volume":"276","author":"Muir","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0115","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1210\/en.2004-1353","article-title":"Effects of KiSS-1 peptide, the natural ligand of GPR54, on follicle stimulating hormone secretion in rat","volume":"146","author":"Navarro","year":"2005","journal-title":"Endocrinology"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0120","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.ygcen.2006.09.008","article-title":"Temporal expression of G-protein-coupled receptor 54 (GPR54) gonadotropin-releasing hormones (GnRH), and dopamine receptor D2 (drd2) in pubertal female grey mullet, Mugil cephalus","volume":"150","author":"Nocillado","year":"2007","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0125","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1210\/er.2009-0005","article-title":"Kisspeptin signaling in the brain","volume":"30","author":"Oakley","year":"2009","journal-title":"Endocr. Rev."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0130","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0074-7696(06)52003-0","article-title":"Cellular and molecular biology of orphan G protein-coupled receptors","volume":"252","author":"Oh","year":"2006","journal-title":"Int. Rev. Cytol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0135","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.ygcen.2013.07.016","article-title":"Identification, characterization and expression profiles of two subtypes of kisspeptin receptors in a scombroid fish (chub mackerel)","volume":"193","author":"Ohga","year":"2013","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0140","doi-asserted-by":"crossref","first-page":"3613","DOI":"10.1210\/en.2004-0395","article-title":"Laser-captured single digoxigenin-labeled neurons of gonadotropin-releasing hormone types reveal a novel G proteincoupled receptor (Gpr54) during maturation in cichlid fish","volume":"145","author":"Parhar","year":"2004","journal-title":"Endocrinology"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0145","first-page":"1472","article-title":"Conserving the endangered mahseers (Tor spp.) of India: the positive role of recreational fisheries","volume":"104","author":"Pinder","year":"2013","journal-title":"Curr. Sci."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0150","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.cbpb.2015.10.008","article-title":"Molecular characterization of two kiss genes and their expression in rohu (Labeo rohita) during annual reproductive cycle","volume":"191","author":"Saha","year":"2016","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0155","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1146\/annurev.pharmtox.46.120604.141218","article-title":"Molecular mechanism of 7TM receptor activation-a global toggle switch model","volume":"46","author":"Schwartz","year":"2006","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0160","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ygcen.2010.07.011","article-title":"Molecular characterization, tissue distribution, and mRNA expression profiles of two kiss genes in adult male and female chub mackerel (Scomber japonicas) during different gonadal stages","volume":"169","author":"Selvaraj","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0165","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/1477-7827-10-64","article-title":"Increased expression of kisspeptin and GnRH forms in the brain of scombroid fish during final ovarian maturation and ovulation","volume":"10","author":"Selvaraj","year":"2012","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0170","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.ygcen.2014.09.019","article-title":"Expression changes of mRNAs encoding kisspeptins and their receptors and gonadotropin-releasing hormones during early development and gonadal sex differentiation periods in the brain of chub mackerel (Scomber japonicus)","volume":"222","author":"Selvaraj","year":"2015","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0175","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1007\/s10695-015-0057-6","article-title":"Spermatogenesis and related plasma androgen and progestin level in wild male golden mahseer, Tor putitora (Hamilton, 1822), during the spawning season","volume":"41","author":"Shahi","year":"2015","journal-title":"Fish Physiol. Biochem."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0180","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ygcen.2010.07.008","article-title":"Elevation of Kiss2 and its receptor gene expression in the brain and pituitary of grass puffer during the spawning season","volume":"169","author":"Shahjahan","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0185","doi-asserted-by":"crossref","first-page":"2976","DOI":"10.1210\/en.2005-0323","article-title":"Differential regulation of kiss-1 mRNA expression by sex steroids in the brain of male mouse","volume":"146","author":"Smith","year":"2005","journal-title":"Endocrinology"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0190","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1210\/en.2007-1425","article-title":"Kisspeptin is present in ovine hypophysial portal blood but does not increase during the preovulatory luteinizing hormone surge: evidence that gonadotropes are not direct targets of kisspeptin in vivo","volume":"149","author":"Smith","year":"2008","journal-title":"Endocrinology"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0195","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.anireprosci.2007.11.005","article-title":"Direct kisspeptin-10 stimulation on luteinizing hormone secretion from bovine and porcine anterior pituitary cells","volume":"105","author":"Suzuki","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0200","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: molecular evolutionary genetics analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0205","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.ygcen.2009.05.004","article-title":"Control of puberty in farmed fish","volume":"165","author":"Taranger","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0210","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1093\/humupd\/dml023","article-title":"GPR54 and kisspeptin in reproduction","volume":"12","author":"Tena-Sempere","year":"2006","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0215","series-title":"8th International Symposium on Fish Endocrinology, 2016","first-page":"73","article-title":"Kisspeptins and their receptors are differentially expressed in the brain-pituitary-gonadal axis during gametogenesis in Odontesthes bonariensis","author":"Tovar","year":"2016"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0220","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.peptides.2007.10.018","article-title":"Evidence for the existence of a functional Kiss1\/Kiss1 receptor pathway in fish","volume":"29","author":"van Aerle","year":"2008","journal-title":"Peptides"},{"key":"10.1016\/j.cbpb.2016.11.010_bb0225","article-title":"Methods of assessing ovarian development in fishes: a review","volume":"4","author":"West","year":"1990","journal-title":"Aust. J. Fresh. Res."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0230","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.ygcen.2009.06.001","article-title":"Goldfish kisspeptin: molecular cloning, tissue distribution of transcript expression, and stimulatory effects on prolaction, growth hormone and luteinizing hormone secretion and gene expression via direct actions at the pituitary level","volume":"165","author":"Yang","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0235","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1095\/biolreprod.111.097667","article-title":"Differential and gonad stage-dependent roles of kisspeptin 1 and kisspepetin 2 in reproduction in the modern teleosts, Morone species","volume":"86","author":"Zmora","year":"2012","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0240","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1095\/biolreprod.115.131870","article-title":"Kisspeptin antagonists reveal kisspeptin 1 and kisspeptin 2 differential regulation of reproduction in teleost, Morone saxatilis","volume":"93","author":"Zmora","year":"2015","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cbpb.2016.11.010_bb0245","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.ygcen.2009.04.017","article-title":"Neuroendocrinology of reproduction in teleost fish","volume":"165","author":"Zohar","year":"2009","journal-title":"Gen. Comp. Endocrinol."}],"container-title":["Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096495916301774?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1096495916301774?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T04:20:15Z","timestamp":1536207615000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1096495916301774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":49,"alternative-id":["S1096495916301774"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpb.2016.11.010","relation":{},"ISSN":["1096-4959"],"issn-type":[{"value":"1096-4959","type":"print"}],"subject":[],"published":{"date-parts":[[2017,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular cloning, characterization and expression profile of kisspeptin1 and kisspeptin1 receptor at brain-pituitary-gonad (BPG) axis of golden mahseer, Tor putitora (Hamilton, 1822) during gonadal development","name":"articletitle","label":"Article Title"},{"value":"Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbpb.2016.11.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}