{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T15:09:30Z","timestamp":1793459370103,"version":"4.5.0"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology"],"published-print":{"date-parts":[[2027,1]]},"DOI":"10.1016\/j.cbpb.2026.111285","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:37:21Z","timestamp":1788950241000},"page":"111285","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Dual effects of mucus from the Japanese flounder (Paralichthys olivaceus) on blood coagulation: procoagulant and anticoagulant activities"],"prefix":"10.1016","volume":"287","author":[{"given":"Shintaro","family":"Matsui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cbpb.2026.111285_bb0005","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1111\/jth.13968","article-title":"Evolutionary conservation of the allosteric activation of factor VIIa by tissue factor in lamprey","volume":"16","author":"Beeler","year":"2018","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.cbpb.2026.111285_bb0010","doi-asserted-by":"crossref","first-page":"3172","DOI":"10.1182\/blood-2010-06-290460","article-title":"Cell-derived vesicles exposing coagulant tissue factor in saliva","volume":"117","author":"Berckmans","year":"2011","journal-title":"Blood"},{"key":"10.1016\/j.cbpb.2026.111285_bb0015","first-page":"693","article-title":"Effect of saliva upon blood coagulation","volume":"90","author":"Borsay","year":"1949","journal-title":"Orv. Hetil."},{"key":"10.1016\/j.cbpb.2026.111285_bb0020","doi-asserted-by":"crossref","first-page":"2056","DOI":"10.1039\/C5MB00890E","article-title":"Immune relevant molecules identified in the skin mucus of fish using -omics technologies","volume":"12","author":"Brinchmann","year":"2016","journal-title":"Mol. BioSyst."},{"key":"10.1016\/j.cbpb.2026.111285_bb0025","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3390\/fishes4010002","article-title":"Mucosal barrier functions of fish under changing environmental conditions","volume":"4","author":"Cabillon","year":"2019","journal-title":"Fishes"},{"key":"10.1016\/j.cbpb.2026.111285_bb0030","first-page":"72","article-title":"Epidermal mucus, a major determinant in fish health: a review","volume":"19","author":"Dash","year":"2018","journal-title":"Iran. J. Vet. Res."},{"key":"10.1016\/j.cbpb.2026.111285_bb0035","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1111\/jth.15529","article-title":"Thrombin generation assays are versatile tools in blood coagulation analysis: a review of technical features, and applications from research to laboratory routine","volume":"19","author":"Depasse","year":"2021","journal-title":"J. Thromb. Haemost."},{"key":"10.1016\/j.cbpb.2026.111285_bb0040","doi-asserted-by":"crossref","first-page":"350","DOI":"10.3390\/md21060350","article-title":"Fish skin mucus extracts: an underexplored source of antimicrobial agents","volume":"21","author":"D\u00edaz-Puertas","year":"2023","journal-title":"Mar. Drugs"},{"key":"10.1016\/j.cbpb.2026.111285_bb0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.jchromb.2019.121965","article-title":"Proteomic profiling of salmon skin mucus for the comparison of sampling methods","volume":"1138","author":"F\u00e6ste","year":"2020","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.cbpb.2026.111285_bb0050","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.fsi.2021.01.001","article-title":"Proteomic profile and protease activity in the skin mucus of greater amberjack (Seriola dumerili) infected with the ectoparasite Neobenedenia girellae - an immunological approach","volume":"110","author":"Fern\u00e1ndez-Montero","year":"2021","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.cbpb.2026.111285_bb0055","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1645\/0022-3395(2000)086[1199:COPITS]2.0.CO;2","article-title":"Characterization of proteases in the skin mucus of Atlantic salmon (Salmo salar) infected with the salmon louse (Lepeophtheirus salmonis) and in whole-body louse homogenate","volume":"86","author":"Firth","year":"2000","journal-title":"J. Parasitol."},{"key":"10.1016\/j.cbpb.2026.111285_bb0060","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1010534","article-title":"Genetic duplication of tissue factor reveals subfunctionalization in venous and arterial hemostasis","volume":"18","author":"Grzegorski","year":"2022","journal-title":"PLoS Genet."},{"key":"10.1016\/j.cbpb.2026.111285_bb0065","article-title":"Species-specific impacts of suspended sediments on gill structure and function in coral reef fishes","volume":"284","author":"Hess","year":"2017","journal-title":"Proc. R. Soc. B Biol. Sci."},{"key":"10.1016\/j.cbpb.2026.111285_bb0070","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1182\/blood-2017-02-765206","article-title":"Genome editing of factor X in zebrafish reveals unexpected tolerance of severe defects in the common pathway","volume":"130","author":"Hu","year":"2017","journal-title":"Blood"},{"key":"10.1016\/j.cbpb.2026.111285_bb0075","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1006\/bcmd.1999.0249","article-title":"Analysis of blood coagulation in the zebrafish","volume":"25","author":"Jagadeeswaran","year":"1999","journal-title":"Blood Cells Mol. Dis."},{"key":"10.1016\/j.cbpb.2026.111285_bb0080","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1042\/bj1410555","article-title":"Affinity chromatography of bovine trypsin: a rapid separation of bovine alpha- and beta-trypsin","volume":"141","author":"Jameson","year":"1974","journal-title":"Biochem. J."},{"key":"10.1016\/j.cbpb.2026.111285_bb0085","doi-asserted-by":"crossref","first-page":"7527","DOI":"10.1073\/pnas.0932632100","article-title":"The evolution of vertebrate blood coagulation as viewed from a comparison of puffer fish and sea squirt genomes","volume":"100","author":"Jiang","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cbpb.2026.111285_bb0090","doi-asserted-by":"crossref","first-page":"591","DOI":"10.2331\/suisan.52.591","article-title":"Clotting time of common carp blood","volume":"52","author":"Kawatsu","year":"1986","journal-title":"Bull. Jpn. Soc. Sci. Fish."},{"key":"10.1016\/j.cbpb.2026.111285_bb0095","doi-asserted-by":"crossref","first-page":"619","DOI":"10.2331\/suisan.57.619","article-title":"Effect of oral administration of warfarin on blood coagulation in the common carp","volume":"57","author":"Kawatsu","year":"1991","journal-title":"Nippon Suisan Gakkaishi"},{"key":"10.1016\/j.cbpb.2026.111285_bb0100","doi-asserted-by":"crossref","first-page":"643","DOI":"10.3389\/fimmu.2015.00643","article-title":"Immunotherapies targeting fish mucosal immunity - current knowledge and future perspectives","volume":"6","author":"Koshio","year":"2016","journal-title":"Front. Immunol."},{"key":"10.1016\/j.cbpb.2026.111285_bb0105","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.thromres.2023.08.012","article-title":"Activated protein C, protein S, and tissue factor pathway inhibitor cooperate to inhibit thrombin activation","volume":"230","author":"Li","year":"2023","journal-title":"Thromb. Res."},{"key":"10.1016\/j.cbpb.2026.111285_bb0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2019.103397","article-title":"Extracellular vesicles from cod (Gadus morhua L.) mucus contain innate immune factors and deiminated protein cargo","volume":"99","author":"Magnad\u00f3ttir","year":"2019","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.cbpb.2026.111285_bb0120","doi-asserted-by":"crossref","DOI":"10.3389\/fmars.2020.592383","article-title":"Unraveling the molecular evolution of blood coagulation genes in fishes and cetaceans","volume":"7","author":"Mariz","year":"2020","journal-title":"Front. Mar. Sci."},{"key":"10.1016\/j.cbpb.2026.111285_bb0125","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1645\/19-21","article-title":"D-mannose-specific immunoglobulin M in grass puffer (Takifugu niphobles), a nonhost fish of a monogenean ectoparasite Heterobothrium okamotoi, can act as a trigger for its parasitism","volume":"106","author":"Matsui","year":"2020","journal-title":"J. Parasitol."},{"key":"10.1016\/j.cbpb.2026.111285_bb0130","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s00360-022-01472-3","article-title":"Unique properties of prothrombin in the bullhead shark Heterodontus japonicus: the first report of purification and characterization of a blood coagulation factor in Chondrichthyes","volume":"193","author":"Matsui","year":"2023","journal-title":"J. Comp. Physiol. B."},{"key":"10.1016\/j.cbpb.2026.111285_bb0135","doi-asserted-by":"crossref","first-page":"205","DOI":"10.2331\/fishsci.63.205","article-title":"Laboratory observation on the nocturnal activity of hatchery-reared juvenile Japanese flounder Paralichthys olivaceus","volume":"63","author":"Miyazaki","year":"1997","journal-title":"Fish. Sci."},{"key":"10.1016\/j.cbpb.2026.111285_bb0140","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.cbpc.2004.03.001","article-title":"Purification and properties of proteinaceous trypsin inhibitors in the skin mucus of pufferfish Takifugu pardalis","volume":"138","author":"Nagashima","year":"2004","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.cbpb.2026.111285_bb0145","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s44313-024-00040-8","article-title":"Back to basics: the coagulation pathway","volume":"59","author":"Park","year":"2024","journal-title":"Blood Res."},{"key":"10.1016\/j.cbpb.2026.111285_bb0150","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1007\/s10695-023-01251-z","article-title":"Isolation and characterization of the morphology, size and particle number of rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio) cell line derived large and small extracellular vesicles","volume":"49","author":"Pelyhe","year":"2023","journal-title":"Fish Physiol. Biochem."},{"key":"10.1016\/j.cbpb.2026.111285_bb0155","doi-asserted-by":"crossref","first-page":"6135","DOI":"10.1182\/bloodadvances.2020002456","article-title":"The evolution of factor XI and the kallikrein-kinin system","volume":"4","author":"Ponczek","year":"2020","journal-title":"Blood Adv."},{"key":"10.1016\/j.cbpb.2026.111285_bb0160","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0308609","article-title":"In vitro fish mucosal surfaces producing mucin as a model for studying host-pathogen interactions","volume":"19","author":"Quintana-Hayashi","year":"2024","journal-title":"PLoS ONE"},{"key":"10.1016\/j.cbpb.2026.111285_bb0165","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3390\/fishes3040041","article-title":"Biological and ecological roles of external fish mucus: a review","volume":"3","author":"Reverter","year":"2018","journal-title":"Fishes"},{"key":"10.1016\/j.cbpb.2026.111285_bb0170","doi-asserted-by":"crossref","DOI":"10.1016\/j.rpth.2023.100081","article-title":"Fibrinolysis: an illustrated review","volume":"7","author":"Risman","year":"2023","journal-title":"Res. Pract. Thromb. Haemost."},{"key":"10.1016\/j.cbpb.2026.111285_bb0175","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2023.105113","article-title":"Comparison of skin mucus lectins among longtooth grouper Epinephelus bruneus and giant grouper E. Lanceolatus as well as the hybrid, Kue-Tama, and their binding abilities to the skin fluke Benedenia epinepheli","volume":"152","author":"Sakakibara","year":"2024","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.cbpb.2026.111285_bb0180","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1016\/j.fsi.2018.10.078","article-title":"Quantitative proteomic profiling of immune responses to Ichthyophthirius multifiliis in common carp skin mucus","volume":"84","author":"Saleh","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.cbpb.2026.111285_bib236","doi-asserted-by":"crossref","first-page":"8768","DOI":"10.1073\/pnas.131109398","article-title":"Demonstration of the extrinsic coagulation pathway in teleostei: identification of zebrafish coagulation factor VII","volume":"98","author":"Sheehan","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.cbpb.2026.111285_bb0185","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1113\/jphysiol.1984.sp015034","article-title":"The influence of mucus on the diffusion of chloride ions across the oesophagus of the minnow (Phoxinus phoxinus L.)","volume":"346","author":"Shephard","year":"1984","journal-title":"J. Physiol."},{"key":"10.1016\/j.cbpb.2026.111285_bb0190","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/S1096-4959(03)00178-7","article-title":"Molecular diversity of skin mucus lectins in fish","volume":"136","author":"Suzuki","year":"2003","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.cbpb.2026.111285_bb0195","doi-asserted-by":"crossref","first-page":"2446","DOI":"10.1111\/raq.12443","article-title":"Mechanical induced wounds in fish: a review on models and healing mechanisms","volume":"12","author":"Sveen","year":"2020","journal-title":"Rev. Aquac."},{"key":"10.1016\/j.cbpb.2026.111285_bb0200","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.3390\/ani10081374","article-title":"Quantification of defensive proteins in skin mucus of Atlantic salmon using minimally invasive sampling and high-sensitivity ELISA","volume":"10","author":"Tartor","year":"2020","journal-title":"Animals"},{"key":"10.1016\/j.cbpb.2026.111285_bb0205","doi-asserted-by":"crossref","DOI":"10.3389\/fmars.2020.541853","article-title":"Skin mucus of marine fish as a source for the development of antimicrobial agents","volume":"7","author":"Tiralongo","year":"2020","journal-title":"Front. Mar. Sci."},{"key":"10.1016\/j.cbpb.2026.111285_bb0210","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.dci.2016.03.027","article-title":"Differential expression of skin mucus C-type lectin in two freshwater eel species, Anguilla marmorata and Anguilla japonica","volume":"61","author":"Tsutsui","year":"2016","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.cbpb.2026.111285_bb0215","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1002\/rth2.12130","article-title":"Salivary tissue factor induces thrombin generation in a diurnal rhythm","volume":"2","author":"van der Vorm","year":"2018","journal-title":"Res. Pract. Thromb. Haemost."},{"key":"10.1016\/j.cbpb.2026.111285_bb0225","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.fsi.2018.05.058","article-title":"Kunitz-type serine protease inhibitor is a novel participator in anti-bacterial and anti-inflammatory responses in Japanese flounder (Paralichthys olivaceus)","volume":"80","author":"Xu","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.cbpb.2026.111285_bb0230","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1038\/s41597-022-01821-5","article-title":"High-quality Japanese flounder genome aids in identifying stress-related genes using gene coexpression network","volume":"9","author":"Xu","year":"2022","journal-title":"Sci. Data"},{"key":"10.1016\/j.cbpb.2026.111285_bb0235","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1111\/jth.14023","article-title":"Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets","volume":"16","author":"Yu","year":"2018","journal-title":"J. Thromb. Haemost."}],"container-title":["Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S109649592600093X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S109649592600093X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:48:15Z","timestamp":1792597695000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S109649592600093X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,1]]},"references-count":46,"alternative-id":["S109649592600093X"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpb.2026.111285","relation":{},"ISSN":["1096-4959"],"issn-type":[{"value":"1096-4959","type":"print"}],"subject":[],"published":{"date-parts":[[2027,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dual effects of mucus from the Japanese flounder (Paralichthys olivaceus) on blood coagulation: procoagulant and anticoagulant activities","name":"articletitle","label":"Article Title"},{"value":"Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbpb.2026.111285","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111285"}}