{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T15:29:29Z","timestamp":1759073369820,"version":"3.37.3"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,7,31]],"date-time":"2018-07-31T00:00:00Z","timestamp":1532995200000},"content-version":"vor","delay-in-days":272,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"published-print":{"date-parts":[[2017,11]]},"DOI":"10.1016\/j.cbpc.2017.07.003","type":"journal-article","created":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T12:01:26Z","timestamp":1500984086000},"page":"12-18","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Characterization of MXR activity in the sea anemone Bunodosoma cangicum exposed to copper"],"prefix":"10.1016","volume":"202","author":[{"given":"Vanessa Abelaira","family":"Anjos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Zomer","family":"Sandrini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia Bueno Reis","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta Marques","family":"Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.cbpc.2017.07.003_bb0005","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.aquatox.2004.01.014","article-title":"Induction of a multixenobiotic resistance protein (MXR) in the asiatic clam Corbicula fluminea after heavy metals exposure","volume":"67","author":"Achard","year":"2004","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0010","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.tiv.2013.11.013","article-title":"Cell damage induced by copper: an explant model to study anemone cells","volume":"26","author":"Anjos","year":"2014","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0015","doi-asserted-by":"crossref","DOI":"10.1007\/s00128-016-1911-7","article-title":"Mesodesma mactroides gill cells exposed to copper: does hyposmotic saline increase cytotoxicity or cellular defenses?","author":"Anjos","year":"2016","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0020","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/S0166-445X(00)00088-6","article-title":"Multixenobiotic resistance as a cellular defense mechanism in aquatic organisms","volume":"48","author":"Bard","year":"2000","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0025","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1002\/cyto.10080","article-title":"Mitochondrial and nonmitochondrial reduction of MTT: interaction of MTT with TMRE, JC-1, and NAO mitochondrial fluorescent probes","volume":"47","author":"Bernas","year":"2002","journal-title":"Cytometry"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0030","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.aquatox.2014.01.001","article-title":"First evidence for toxic defense based on the multixenobiotic resistance (MXR) mechanism in Daphnia magna","volume":"148","author":"Campos","year":"2014","journal-title":"Aquat. Toxicol."},{"author":"CONAMA","key":"10.1016\/j.cbpc.2017.07.003_bb0035"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0040","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cbi.2012.04.008","article-title":"Interaction of ABC transport proteins with toxic metals at the level of gene and transport activity in the PLHC-1 fish cell line","volume":"198","author":"Della Torre","year":"2012","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0045","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.marenvres.2013.06.005","article-title":"Differential ABCB and ABCC gene expression and efflux activities in gills and hemocytes of Mytilus galloprovincialis and their involvement in cadmium response","volume":"93","author":"Della Torre","year":"2014","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0050","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S1095-6433(98)10005-3","article-title":"Use of multidrug transporters as first lines of defense against toxins in aquatic organisms","volume":"120","author":"Epel","year":"1998","journal-title":"Comp. Biochem. Physiol. A"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0055","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2014.00266","article-title":"ABC transporters in fish species: a review","volume":"5","author":"Ferreira","year":"2014","journal-title":"Front. Physiol."},{"year":"2010","series-title":"Cytotoxicity \u2013 Chapter 21, in Culture of Animals Cells: A Manual of Basic Technique and Specialized Applications","author":"Freshney","key":"10.1016\/j.cbpc.2017.07.003_bb0060"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0065","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0300-483X(03)00159-8","article-title":"Copper toxicity, oxidative stress, and antioxidant nutrients","volume":"189","author":"Gaetke","year":"2003","journal-title":"Toxicology"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0070","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1038\/nature02263","article-title":"Protein degradation and protection against misfolded or damage proteins","volume":"426","author":"Goldberg","year":"2003","journal-title":"Nature"},{"issue":"A","key":"10.1016\/j.cbpc.2017.07.003_bb0075","first-page":"53","article-title":"Copper, in homeostasis and toxicology of essential metals","volume":"31","author":"Grosell","year":"2012","journal-title":"Fish Physiol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0080","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1242\/jeb.205.8.1179","article-title":"Copper uptake across rainbow trout gills: mechanisms of apical entry","volume":"205","author":"Grosell","year":"2002","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0085","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.aquatox.2014.08.005","article-title":"Functional characterization of P-glycoprotein in the intertidal copepod Tigriopus japonicus and its potential role in remediating metal pollution","volume":"156","author":"Jeong","year":"2014","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0090","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1023\/A:1026560922731","article-title":"Multixenobiotic resistance, P-glycoprotein and chemosensitizers","volume":"9","author":"Kurelec","year":"2000","journal-title":"Ecotoxicology"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0095","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.jembe.2004.03.007","article-title":"Development of a bioassay from isolated digestive gland cells of the cuttlefish Sepia officinalis L. (Mollusca Cephalopoda): effect of Cu, Zn and Ag on enzyme activities and cell viability","volume":"309","author":"Le Bihan","year":"2004","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0100","first-page":"216","article-title":"Copper accumulation and oxidative stress in the sea anemone, Aiptasia pallida, after waterborne copper exposure","volume":"151","author":"Main","year":"2010","journal-title":"Comp. Biochem. Physiol. C"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0105","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0166-445X(96)00801-6","article-title":"Relationship between copper exposure duration, tissue copper accumulation, and rainbow trout growth","volume":"36","author":"Marr","year":"1996","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0110","first-page":"19","article-title":"Amplia\u00e7\u00e3o da distribui\u00e7\u00e3o das an\u00eamonas-do-mar (Cnidaria, Actiniaria) no estado de Pernambuco","volume":"33","author":"Melo","year":"2005","journal-title":"Brasil. Trop. Ocean"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0115","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0120","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/s00244-011-9675-x","article-title":"The influence of lead on different proteins in gill cells from the freshwater bivalve, Corbicula fluminea, from defense to repair biomarkers","volume":"62","author":"Rocha","year":"2012","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0125","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.cbi.2009.08.014","article-title":"Superoxide anion mediated mitochondrial dysfunction leads to hepatocyte apoptosis preferentially in the periportal region during copper toxicity in rats","volume":"182","author":"Roy","year":"2009","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0130","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0166-445X(96)00805-3","article-title":"Detoxification of exogenous copper by binding to hemolymph proteins in the shore crab, Carcinus mamas","volume":"36","author":"Rtal","year":"1996","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0135","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1016\/j.chemosphere.2006.11.017","article-title":"Identification of multixenobiotic defense mechanism (MXR) background activities in the freshwater bivalve Dreissena polymorpha as reference values for its use as biomarker in contaminated ecosystems","volume":"67","author":"Sandrine","year":"2007","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2017.07.003_bb0140","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.aquatox.2009.02.016","article-title":"Reactive oxygen species generation and expression of DNA repair-related genes after copper exposure in zebrafish (Danio rerio) ZFL cells","volume":"95","author":"Sandrini","year":"2009","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0145","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.aquatox.2005.03.014","article-title":"Identification of a multixenobiotic resistance mechanism in primary cultured epidermal cells from Oncorhynchus mykiss and the effects of environmental complex mixtures on its activity","volume":"73","author":"Sh\u00failleabh\u00e1in","year":"2005","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbrc.2008.05.052","article-title":"Heavy metals ions are potent inhibitors of protein folding","volume":"372","author":"Sharma","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0155","doi-asserted-by":"crossref","DOI":"10.1590\/S1984-82502012000300002","article-title":"Overview of P-glycoprotein inhibitors: a rational outlook","volume":"48","author":"Srivalli","year":"2012","journal-title":"Braz. J. Pharm. Sci."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0160","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.acthis.2012.01.006","article-title":"MTT assay for cell viability: intracellular localization of the formazan product is in lipid droplets","volume":"114","author":"Stockert","year":"2012","journal-title":"Acta Histochem."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0165","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0891-5849(94)00159-H","article-title":"Oxidative mechanisms in the toxicity of metals ions","volume":"18","author":"Stohs","year":"1995","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0170","article-title":"Heavy metals and metalloids as a cause for protein misfolding and aggregation","volume":"4","author":"Tam\u00e1s","year":"2014","journal-title":"Biomol. Ther."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0175","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1002\/etc.5620190920","article-title":"Physiological effects of chronic copper exposure to rainbow trout (Oncorhynchus mykiss) in hard and soft water: evaluation of chronic indicators","volume":"19","author":"Taylor","year":"2000","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0180","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.ecoenv.2014.01.018","article-title":"In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax","volume":"102","author":"Vazzana","year":"2014","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0185","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.aquatox.2009.05.003","article-title":"P-glycoprotein (multi-xenobiotic resistance) and heat shock protein gene expression in the reef coral Montastraea franksi in response to environmental toxicants","volume":"93","author":"Venn","year":"2009","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2017.07.003_bb0190","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.neuropharm.2006.07.037","article-title":"ATP depletion does not account for apoptosis induced by inhibition of mitochondrial electron transport chain in human dopaminergic cells","volume":"52","author":"Watabe","year":"2007","journal-title":"Neuropharmacology"}],"container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045617301345?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045617301345?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T08:06:10Z","timestamp":1657699570000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1532045617301345"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11]]},"references-count":38,"alternative-id":["S1532045617301345"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpc.2017.07.003","relation":{},"ISSN":["1532-0456"],"issn-type":[{"type":"print","value":"1532-0456"}],"subject":[],"published":{"date-parts":[[2017,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of MXR activity in the sea anemone Bunodosoma cangicum exposed to copper","name":"articletitle","label":"Article Title"},{"value":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbpc.2017.07.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Inc.","name":"copyright","label":"Copyright"}]}}