{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T15:07:33Z","timestamp":1784300853988,"version":"3.55.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["480708\/2013-4"],"award-info":[{"award-number":["480708\/2013-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["573949\/2008-5"],"award-info":[{"award-number":["573949\/2008-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"published-print":{"date-parts":[[2018,4]]},"DOI":"10.1016\/j.cbpc.2018.02.003","type":"journal-article","created":{"date-parts":[[2018,2,20]],"date-time":"2018-02-20T02:14:24Z","timestamp":1519092864000},"page":"17-22","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":35,"special_numbering":"C","title":["The herbicide atrazine affects sperm quality and the expression of antioxidant and spermatogenesis genes in zebrafish testes"],"prefix":"10.1016","volume":"206-207","author":[{"given":"Felix Esteban Airahuacho","family":"Bautista","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Sergio","family":"Varela Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carine Dahl","family":"Corcini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izani Bonel","family":"Acosta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergiane Souza","family":"Caldas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ednei Gilberto","family":"Primel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliano","family":"Zanette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.cbpc.2018.02.003_bb0005","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1007\/s00244-009-9371-2","article-title":"Changes in sperm characteristics and induction of oxidative stress in the testis and epididymis of experimental rats by herbicide, atrazine","volume":"58","author":"Abarikwu","year":"2010","journal-title":"Arch. Environ. Contam. Toxicol."},{"issue":"2","key":"10.1016\/j.cbpc.2018.02.003_bb0010","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.1439-0531.2008.01198.x","article-title":"The advantages of combining low-density lipoproteins with glutamine for cryopreservation of canine semen","volume":"45","author":"Bencharif","year":"2010","journal-title":"Reprod. Domest. Anim."},{"issue":"3","key":"10.1016\/j.cbpc.2018.02.003_bb0015","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.reprotox.2006.03.001","article-title":"Effect of two insecticides and two herbicides on the porcine sperm motility patterns using computer-assisted semen analysis (CASA) in vitro","volume":"22","author":"Betancourt","year":"2006","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.cbpc.2018.02.003_bb0020","series-title":"Conselho Nacional do Meio Ambiente\/Minist\u00e9rio do Meio Ambiente. Resolu\u00e7\u00e3o N\u00b0 357 de 17 de marco de 2005","author":"CONAMA","year":"2005"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0025","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/bdrb.20110","article-title":"Atrazine and reproductive function: mode and mechanism of action studies","volume":"80","author":"Cooper","year":"2007","journal-title":"Birth Defects Res. B Dev. Reprod. Toxicol."},{"issue":"1","key":"10.1016\/j.cbpc.2018.02.003_bb0030","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1023\/B:AQUI.0000017189.44263.bc","article-title":"The ionic and osmotic factors controlling motility of fish spermatozoa","volume":"12","author":"Cosson","year":"2004","journal-title":"Aquac. Int."},{"key":"10.1016\/j.cbpc.2018.02.003_bb8000","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1071\/MF9940209","article-title":"Triazine herbicide contamination of Tasmanian streams: sources, concentrations and effects on biota","volume":"45","author":"Davies","year":"1994","journal-title":"Aust. J. Mar. Freshwat. Res."},{"issue":"8","key":"10.1016\/j.cbpc.2018.02.003_bb0035","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1590\/S0103-50532010000800003","article-title":"Development and validation of a method using SPE and LC-ESI-MS-MS for the determination of multiple classes of pesticides and metabolites in water samples","volume":"21","author":"Demoliner","year":"2010","journal-title":"J. Braz. Chem. Soc."},{"issue":"3","key":"10.1016\/j.cbpc.2018.02.003_bb9000","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.chemosphere.2009.06.052","article-title":"Effects of atrazine on cytochrome P450 enzymes of zebrafish (Danio rerio)","volume":"77","author":"Dong","year":"2009","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0040","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1111\/and.12001","article-title":"Quercetin exacerbates the effects of subacute treatment of atrazine on reproductive tissue antioxidant defense system, lipid peroxidation and sperm quality in rats","volume":"45","author":"Farombi","year":"2013","journal-title":"Andrologia"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0045","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/S0160-4120(01)00031-9","article-title":"Impacts of atrazine in aquatic ecosystems","volume":"26","author":"Graymore","year":"2001","journal-title":"Environ. Int."},{"issue":"6","key":"10.1016\/j.cbpc.2018.02.003_bb6000","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0039397","article-title":"Oxidative Stress in Zebrafish (Danio rerio) Sperm","volume":"7","author":"Hagedorn","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0050","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1530\/jrf.0.0880343","article-title":"Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa","volume":"88","author":"Harrison","year":"1990","journal-title":"J. Reprod. Fertil."},{"key":"10.1016\/j.cbpc.2018.02.003_bb0055","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.bbrc.2007.11.107","article-title":"Atrazine binds to F1F0-ATP synthase and inhibits mitochondrial function in sperm","volume":"366","author":"Hase","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.cbpc.2018.02.003_bb0060","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1289\/ehp.5932","article-title":"Atrazine-induced hermaphroditism at 0.1\u202fppb in American leopard frogs (Rana pipiens): laboratory and field evidence","volume":"111","author":"Hayes","year":"2003","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.cbpc.2018.02.003_bb0065","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.cryobiol.2004.01.009","article-title":"Effects of dimethyl sulfoxide and glycine on cryopreservation induced damage of plasma membranes and mitochondria to striped bass (Morone saxatilis) sperm","volume":"48","author":"He","year":"2004","journal-title":"Cryobiology"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0070","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.anireprosci.2014.01.012","article-title":"Genome-wide association study for poor sperm motility in Holstein-Friesian bulls","volume":"146","author":"Hering","year":"2014","journal-title":"Anim. Reprod. Sci."},{"issue":"33","key":"10.1016\/j.cbpc.2018.02.003_bb0075","doi-asserted-by":"crossref","first-page":"24397","DOI":"10.1074\/jbc.M701603200","article-title":"Involvement of cystic fibrosis transmembrane conductance regulator in mouse sperm capacitation","volume":"282","author":"Hernandez-Gonzalez","year":"2007","journal-title":"J. Biol. Chem."},{"issue":"3","key":"10.1016\/j.cbpc.2018.02.003_bb0080","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/j.1439-0531.2009.01363.x","article-title":"Is semen analysis useful to predict the odds that the sperm will meet the egg?","volume":"44","author":"Holt","year":"2009","journal-title":"Reprod. Domest. Anim."},{"issue":"4","key":"10.1016\/j.cbpc.2018.02.003_bb0085","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/S0093-691X(03)00050-5","article-title":"Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility","volume":"60","author":"Januskauskas","year":"2003","journal-title":"Theriogenology"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0090","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1016\/j.chemosphere.2009.11.044","article-title":"Oxidative stress response and gene expression with atrazine exposure in adult female zebrafish (Danio rerio)","volume":"78","author":"Jin","year":"2010","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0095","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1095\/biolreprod.109.076299","article-title":"Histological and stereological evaluation of zebrafish (Danio rerio) spermatogenesis with an emphasis on spermatogonial generations","volume":"81","author":"Leal","year":"2009","journal-title":"Biol. Reprod."},{"issue":"3","key":"10.1016\/j.cbpc.2018.02.003_bb5000","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1093\/molehr\/6.3.226","article-title":"Fatty acid composition of spermatozoa and immature germ cells","volume":"6","author":"Lenzi","year":"2000","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.cbpc.2018.02.003_bb0100","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.aquatox.2014.07.006","article-title":"Effect of glyphosate on the sperm quality of zebrafish Danio rerio","volume":"155","author":"Lopes","year":"2014","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2018.02.003_bb0105","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.anireprosci.2017.08.001","article-title":"Sperm quality variables as indicators of bull fertility may be breed dependent","volume":"185","author":"Morrell","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cbpc.2018.02.003_bb0110","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.dnarep.2015.09.004","article-title":"XPC: going where no DNA damage sensor has gone before","volume":"36","author":"Nemzow","year":"2015","journal-title":"DNA Repair"},{"issue":"10","key":"10.1016\/j.cbpc.2018.02.003_bb0115","doi-asserted-by":"crossref","first-page":"3804","DOI":"10.1210\/en.2015-1157","article-title":"Fsh stimulates spermatogonial proliferation and differentiation in zebrafish via Igf3","volume":"156","author":"N\u00f3brega","year":"2015","journal-title":"Endocrinology"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0120","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.aquatox.2014.05.022","article-title":"Atrazine reduces reproduction in Japanese medaka (Oryzias latipes)","volume":"154","author":"Papoulias","year":"2014","journal-title":"Aquat. Toxicol."},{"issue":"5","key":"10.1016\/j.cbpc.2018.02.003_bb0125","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1093\/humupd\/dmp016","article-title":"Mitochondrial functionality in reproduction: from gonads and gametes to embryos and embryonic stem cells","volume":"15","author":"Ramalho-Santos","year":"2009","journal-title":"Hum. Reprod. Update"},{"issue":"3","key":"10.1016\/j.cbpc.2018.02.003_bb7000","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/j.1439-0272.1992.tb02627.x","article-title":"Sperm plasma membrane integrity in fertile and infertile men","volume":"24","author":"Ramirez","year":"1992","journal-title":"Andrologia"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0130","doi-asserted-by":"crossref","first-page":"592","DOI":"10.2164\/jandrol.111.014266","article-title":"Androgen action in the epididymis","volume":"32","author":"Robaire","year":"2011","journal-title":"J. Androl."},{"key":"10.1016\/j.cbpc.2018.02.003_bb0135","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.ygcen.2016.04.026","article-title":"Molecular basis of spermatogenesis and sperm quality","volume":"245","author":"Robles","year":"2017","journal-title":"Gen. Comp. Endocrinol."},{"issue":"1","key":"10.1016\/j.cbpc.2018.02.003_bb0140","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1289\/ehp.0901164","article-title":"A qualitative meta-analysis reveals consistent effects of atrazine on freshwater fish and amphibians","volume":"118","author":"Rohr","year":"2010","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.cbpc.2018.02.003_bb0145","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aquaculture.2003.12.006","article-title":"The measurement of sperm motility and factors affecting sperm quality in cultured fish","volume":"234","author":"Rurangwa","year":"2004","journal-title":"Aquaculture"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0150","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.anireprosci.2015.10.001","article-title":"Predictive capacity of sperm quality parameters and sperm subpopulations on field fertility after artificial insemination in sheep","volume":"163","author":"Santolaria","year":"2015","journal-title":"Anim. Reprod. Sci."},{"issue":"6","key":"10.1016\/j.cbpc.2018.02.003_bb0155","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative CT method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.cbpc.2018.02.003_bb0160","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.cbpc.2008.10.099","article-title":"Zebrafish (Danio rerio) as a model organism for investigating endocrine disruption","volume":"149","author":"Segner","year":"2009","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"issue":"1","key":"10.1016\/j.cbpc.2018.02.003_bb0165","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1007\/s00128-015-1686-2","article-title":"Estimated levels of environmental contamination and health risk assessment for herbicides and insecticides in surface water of Cear\u00e1, Brazil","volume":"96","author":"Sousa","year":"2016","journal-title":"Bull. Environ. Contam. Toxicol."},{"issue":"5","key":"10.1016\/j.cbpc.2018.02.003_bb0170","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0002117","article-title":"The herbicide atrazine activates endocrine gene networks via non-steroidal NR5A nuclear receptors in fish and mammalian cells","volume":"3","author":"Suzawa","year":"2008","journal-title":"PLoS One"},{"key":"10.1016\/j.cbpc.2018.02.003_bb0175","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1006\/excr.1998.4064","article-title":"Mitochondrial membrane potential and DNA stainability in human sperm cells: a flow cytometry analysis with implications for male infertility","volume":"241","author":"Troiano","year":"1998","journal-title":"Exp. Cell Res."},{"issue":"23","key":"10.1016\/j.cbpc.2018.02.003_bb0180","doi-asserted-by":"crossref","first-page":"9816","DOI":"10.1073\/pnas.0609253104","article-title":"Cystic fibrosis transmembrane conductance regulator is vital to sperm fertilizing capacity and male fertility","volume":"104","author":"Xu","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"3","key":"10.1016\/j.cbpc.2018.02.003_bb0185","doi-asserted-by":"crossref","first-page":"639","DOI":"10.3892\/mmr.2012.965","article-title":"Variants in the SRD5A2 gene are associated with quality of semen","volume":"6","author":"Zhao","year":"2012","journal-title":"Mol. Med. Rep."},{"issue":"1","key":"10.1016\/j.cbpc.2018.02.003_bb0190","doi-asserted-by":"crossref","first-page":"31","DOI":"10.3109\/15376516.2010.529186","article-title":"DNA damage and effects on antioxidative enzymes in zebra fish (Danio rerio) induced by atrazine","volume":"21","author":"Zhu","year":"2011","journal-title":"Toxicol. Mech. Methods"}],"container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045618300139?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045618300139?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T00:24:08Z","timestamp":1535070248000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1532045618300139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4]]},"references-count":43,"alternative-id":["S1532045618300139"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpc.2018.02.003","relation":{},"ISSN":["1532-0456"],"issn-type":[{"value":"1532-0456","type":"print"}],"subject":[],"published":{"date-parts":[[2018,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The herbicide atrazine affects sperm quality and the expression of antioxidant and spermatogenesis genes in zebrafish testes","name":"articletitle","label":"Article Title"},{"value":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbpc.2018.02.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}