{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:47:17Z","timestamp":1790398037739,"version":"4.1.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100008638","name":"Environment and Climate Change Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008638","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"published-print":{"date-parts":[[2021,6]]},"DOI":"10.1016\/j.cbpc.2021.109027","type":"journal-article","created":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T20:32:27Z","timestamp":1614025947000},"page":"109027","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Occurrence and ecotoxicity of cytostatic drugs 5-fluorouracil and methotrexate in the freshwater unionid Elliptio complanata"],"prefix":"10.1016","volume":"244","author":[{"given":"Christine","family":"Kleinert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Poirier-Larabie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Gagnon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantale","family":"Andr\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Gagn\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cbpc.2021.109027_bb0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpc.2020.108760","article-title":"The comparative toxicity of rainfall overflows and a municipal effluent plume in Elliptio complanata using a novel qPCR array approach","volume":"232","author":"Andr\u00e9","year":"2020","journal-title":"Comp Biochem Physiol C Toxicol Pharmacol"},{"key":"10.1016\/j.cbpc.2021.109027_bb0010","first-page":"100","article-title":"Alterations in DNA metabolism in Elliptio complanata mussels after exposure to municipal effluents","volume":"154C","author":"Gagn\u00e9","year":"2011","journal-title":"Comp. Biochem. Physiol."},{"key":"10.1016\/j.cbpc.2021.109027_bb0015","first-page":"73","article-title":"Effects of pharmaceuticals on aquatic biota - a review","volume":"1","author":"Gagn\u00e9","year":"2004","journal-title":"Cur Top Toxicol"},{"key":"10.1016\/j.cbpc.2021.109027_bb0020","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1016\/j.chemosphere.2004.05.010","article-title":"Pharmaceuticals in the aquatic environment\u2013\u2013a comparison of risk assessment strategies","volume":"56","author":"Bound","year":"2004","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2021.109027_bb0025","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.cbpc.2021.109027_bb0030","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1021\/es60098a008","article-title":"Partition coefficient to measure bioconcentration potential of organic chemicals in fish","volume":"8","author":"Neely","year":"1974","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.cbpc.2021.109027_bb0035","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.2134\/jeq2008.0128","article-title":"Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste","volume":"38","author":"Chee-Sanford","year":"2009","journal-title":"J. Environ. Qual."},{"key":"10.1016\/j.cbpc.2021.109027_bb0040","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1007\/s11356-012-0980-3","article-title":"DNA integrity assessment in hemocytes of soft-shell clams (Mya arenaria) in the Saguenay Fjord (Qu\u00e9bec, Canada)","volume":"20","author":"Debenest","year":"2012","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.cbpc.2021.109027_bb0045","doi-asserted-by":"crossref","first-page":"14","DOI":"10.4081\/xeno.2013.s1.e6","article-title":"Genotoxic impact of a municipal effluent dispersion plume in the freshwater mussel Elliptio complanata: an in situ study","volume":"3","author":"Lacaze","year":"2013","journal-title":"J. Xenobiotics"},{"key":"10.1016\/j.cbpc.2021.109027_bb0050","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.envpol.2014.04.024","article-title":"The impact of in vivo and in vitro exposure to base analogue 5-FLU on the level of DNA damage in Haemocytes of freshwater mussels Unio Pictorum and Unio Tumidus","volume":"191","author":"Ga\u010di\u0107","year":"2014","journal-title":"Environ Poll"},{"key":"10.1016\/j.cbpc.2021.109027_bb0055","series-title":"Biochemical Ecotoxicology, Principles and Methods","first-page":"117","article-title":"Xenobiotic biotransformation, Chapt 7","author":"Gagn\u00e9","year":"2014"},{"key":"10.1016\/j.cbpc.2021.109027_bb0060","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.ecoenv.2005.04.004","article-title":"Occurrence of pharmaceutical products in a municipal effluent and toxicity to rainbow trout (Oncorhynchus Mykiss) hepatocytes","volume":"64","author":"Gagn\u00e9","year":"2006","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.cbpc.2021.109027_bb0065","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.marenvres.2016.05.004","article-title":"Toxic effects of cisplatin cytostatic drug in mussel Mytilus Galloprovincialis","volume":"119","author":"Trombini","year":"2016","journal-title":"Marine Environl Res"},{"key":"10.1016\/j.cbpc.2021.109027_bb0070","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.toxlet.2007.10.012","article-title":"Human pharmaceuticals in surface waters: implementation of a prioritization methodology and application to the French situation","volume":"176","author":"Besse","year":"2008","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.cbpc.2021.109027_bb0075","first-page":"69","article-title":"The effects of municipal effluents on oxidative stress, immunocompetence and DNA integrity in fathead minnow juveniles","author":"Lacaze","year":"2017","journal-title":"Cur Top Toxicol 13"},{"key":"10.1016\/j.cbpc.2021.109027_bb0080","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0141-1136(02)00129-0","article-title":"Implications from a field study regarding the relationship between polycyclic aromatic hydrocarbons and glutathione S-transferase activity in mussels","volume":"54","author":"Gowland","year":"2002","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.cbpc.2021.109027_bb0085","first-page":"159","article-title":"Prioritising anticancer drugs for environmental monitoring and risk assessment purposes","volume":"473\u2013474","author":"Booker","year":"2014","journal-title":"STOTEN"},{"key":"10.1016\/j.cbpc.2021.109027_bb0090","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.jhydrol.2007.09.054","article-title":"Do cytotoxic chemotherapy drugs discharged into rivers pose a risk to the environment and human health? An overview and UK case study","volume":"348","author":"Johnson","year":"2008","journal-title":"J. Hydrol."},{"issue":"6\u20137","key":"10.1016\/j.cbpc.2021.109027_bb0095","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/S0045-6535(01)00144-8","article-title":"Drugs in the environment: emission of drugs, diagnostic aids and disinfectants into wastewater by hospitals in relation to other sources \u2013 a review","volume":"45","author":"K\u00fcmmerer","year":"2001","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2021.109027_bb0100","series-title":"Pharmaceuticals in the Environment: Sources, Fate, Effects and Risks","first-page":"3","article-title":"Pharmaceuticals in the environment \u2013 a brief summary","author":"K\u00fcmmerer","year":"2008"},{"key":"10.1016\/j.cbpc.2021.109027_bb0110","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.envint.2011.10.002","article-title":"Anticancer drugs in surface waters: what can we say about the occurrence and environmental significance of cytotoxic, cytostatic and endocrine therapy drugs?","volume":"39","author":"Besse","year":"2012","journal-title":"Environ Intern"},{"key":"10.1016\/j.cbpc.2021.109027_bb0115","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.chemosphere.2006.05.051","article-title":"Fate of 5-fluorouracil, doxorubicin, epirubicin, and daunorubicin in hospital wastewater and their elimination by activated sludge and treatment in a membrane-bio-reactor system","volume":"66","author":"Mahnik","year":"2007","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2021.109027_bb0120","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.etap.2009.04.009","article-title":"The use of biochemical responses to assess ecotoxicological effects of pharmaceutical and personal care products (PPCPs) after injection in the mussel Elliptio Complanata","volume":"28","author":"Mart\u00edn-D\u00edaz","year":"2009","journal-title":"Environ Toxicol Pharm"},{"key":"10.1016\/j.cbpc.2021.109027_bb0180","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1007\/s11270-014-1896-y","article-title":"Ecotoxicological Risk Assessment of 14 Cytostatic Drugs in Wastewater","volume":"225","author":"Mart\u00edn","year":"2014","journal-title":"Water Air Soil Pollut"},{"key":"10.1016\/j.cbpc.2021.109027_bb0125","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1002\/jat.1411","article-title":"5-Fluorouracil and its active metabolite FdUMP cause DNA damage in human SW620 colon adenocarcinoma cell line","volume":"29","author":"Matuo","year":"2009","journal-title":"J. Appl. Toxicol."},{"key":"10.1016\/j.cbpc.2021.109027_bb0130","article-title":"Evaluation of uptake of cytostatic methotrexate and 5-Fluouracil in Elliptio complanata mussels: a novel LC-MS\/MS method","author":"Poirier Larabie","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.cbpc.2021.109027_bb0135","doi-asserted-by":"crossref","first-page":"13481","DOI":"10.1073\/pnas.172501499","article-title":"Interaction of dihydrofolate reductase with methotrexate: ensemble and single-molecule kinetics","volume":"99","author":"Rajagopalan","year":"2002","journal-title":"Proc Nat Acad Sci"},{"key":"10.1016\/j.cbpc.2021.109027_bb0140","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.envres.2015.10.028","article-title":"Is the step-wise tiered approach for ERA of pharmaceuticals useful for the assessment of cancer therapeutic drugs present in marine environment?","volume":"144","author":"Aguirre-Mart\u00ednez","year":"2016","journal-title":"Environl Res"},{"key":"10.1016\/j.cbpc.2021.109027_bb0150","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s00128-009-9884-4","article-title":"A preliminary study on the occurrence of cytostatic drugs in hospital effluents in Beijing, China","volume":"84","author":"Yin","year":"2010","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"10.1016\/j.cbpc.2021.109027_bb0155","doi-asserted-by":"crossref","first-page":"22721","DOI":"10.1074\/jbc.M500277200","article-title":"Identification of amino acids required for the functional up-regulation of human dihydrofolate reductase protein in response to antifolate treatment","volume":"280","author":"Skacel","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.cbpc.2021.109027_bb0160","doi-asserted-by":"crossref","first-page":"6715","DOI":"10.1007\/s11356-016-8352-z","article-title":"Genotoxicity and cytotoxicity reduction of the polluted urban river after ecological restoration: a field-scale study of Jialu River in northern China","volume":"24","author":"Sun","year":"2017","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"10.1016\/j.cbpc.2021.109027_bb0165","first-page":"132","article-title":"The occurrence of acidic drugs and caffeine in sewage effluents and receiving waters from three coastal watersheds in Atlantic Canada","volume":"396","author":"Comeau","year":"2008","journal-title":"STOTEN"},{"key":"10.1016\/j.cbpc.2021.109027_bb0170","first-page":"75","article-title":"Long-term and comparative impacts of combined sewers and municipal effluents to freshwater mussels","volume":"17","author":"Andr\u00e9","year":"2020","journal-title":"Invert Surv J"},{"key":"10.1016\/j.cbpc.2021.109027_bb0185","series-title":"Biochemical toxicology. A practical Approach","first-page":"127","article-title":"Evaluation oflipid peroxidation in lipids and biological membranes","author":"Wills","year":"1987"},{"key":"10.1016\/j.cbpc.2021.109027_bb0175","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1002\/etc.4687","article-title":"Antineoplastic agents: environmental prevalence and adverse outcomes in aquatic organisms","volume":"39","author":"Wormington","year":"2020","journal-title":"Environ. Toxicol. Chem."}],"container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045621000545?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045621000545?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:26:42Z","timestamp":1761517602000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1532045621000545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6]]},"references-count":35,"alternative-id":["S1532045621000545"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpc.2021.109027","relation":{},"ISSN":["1532-0456"],"issn-type":[{"value":"1532-0456","type":"print"}],"subject":[],"published":{"date-parts":[[2021,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Occurrence and ecotoxicity of cytostatic drugs 5-fluorouracil and methotrexate in the freshwater unionid Elliptio complanata","name":"articletitle","label":"Article Title"},{"value":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbpc.2021.109027","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown Copyright \u00a9 2021 Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109027"}}