{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:48:54Z","timestamp":1790635734574,"version":"4.1.0"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T00:00:00Z","timestamp":1643587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"published-print":{"date-parts":[[2022,5]]},"DOI":"10.1016\/j.cbpc.2022.109276","type":"journal-article","created":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T03:35:01Z","timestamp":1643600101000},"page":"109276","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Impacts of sewage sludges deposition on agricultural soils: Effects upon model soil organisms"],"prefix":"10.1016","volume":"255","author":[{"given":"Erik","family":"Urionabarrenetxea","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nerea","family":"Garcia-Velasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Be\u00f1at","family":"Zaldibar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manu","family":"Soto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2014","key":"10.1016\/j.cbpc.2022.109276_bb0005","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.scitotenv.2014.08.085","article-title":"Bioavailability and soil-to-plant transfer factors as indicators of potentially toxic element contamination in agricultural soils","volume":"500\u2013501","author":"Adamo","year":"2014","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109276_bb0020","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s11368-010-0285-4","article-title":"Toxicity assessment using Lactuca sativa L. bioassay of the metal(loid)s As, Cu, Mn, Pb and Zn in soluble-in-water saturated soil extracts from an abandoned mining site","volume":"11","author":"Bagur-Gonz\u00e1lez","year":"2010","journal-title":"J.SoilsSediments"},{"issue":"2\u20133","key":"10.1016\/j.cbpc.2022.109276_bb0025","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/S0048-9697(99)00499-4","article-title":"The use of biomarkers to assess the impact of pollution in coastal environments of the Iberian Peninsula: a practical approach","volume":"247","author":"Cajaraville","year":"2000","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109276_bb0030","series-title":"Introduction And Technical Background, Metals And Inorganics, Pesticides And PCBs. Report to U.S. Environmental Protection Agency","article-title":"Water-related Environmental Fate of 129 Priority Pollutants","volume":"Vol. 1","author":"Callahan","year":"1979"},{"key":"10.1016\/j.cbpc.2022.109276_bb0035","series-title":"Handbook of Methods for Oxygen Radical Research","first-page":"283","article-title":"Catalase activity","author":"Claiborne","year":"1985"},{"key":"10.1016\/j.cbpc.2022.109276_bb0040","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.ecoenv.2017.03.008","article-title":"Responses to silver nanoparticles and silver nitrate in a battery of biomarkers measured in coelomocytes and in target tissues of Eisenia fetida earthworms","volume":"141","author":"Curieses","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.cbpc.2022.109276_bb0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.149479","article-title":"Toxicity assessment of earthworm exposed to arsenate using oxidative stress and burrowing behavior responses and an integrated biomarker index","volume":"800","author":"Deng","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109276_bb0050","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1002\/em.20153","article-title":"Improved single cell gel electrophoresis assay for detecting DNA damage in Eisenia foetida","volume":"46","author":"Di Marzio","year":"2005","journal-title":"Environ. Mol. Mutagen."},{"issue":"2008","key":"10.1016\/j.cbpc.2022.109276_bb0055","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1016\/j.chemosphere.2008.07.061","article-title":"Assessment of heavy metals phytotoxicity using seed germination and root elongation tests: a comparison of two growth substrates","volume":"73","author":"Di Salvatore","year":"2008","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109276_bb0060","series-title":"Ecological Effects Test Guidelines. Seed Germination\/root Elongation Toxicity Test. OPPTS 850.4200","year":"1996"},{"key":"10.1016\/j.cbpc.2022.109276_bb0065","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.chemosphere.2017.04.143","article-title":"Integrative assessment of the effects produced by Ag nanoparticles at different levels of biological complexity in Eisenia fetida mantained in two standard soils (OECD and LUFA 2.3)","volume":"181","author":"Garcia-Velasco","year":"2017","journal-title":"Chemosphere"},{"issue":"2019","key":"10.1016\/j.cbpc.2022.109276_bb0070","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1387\/ekaia.19633","article-title":"Fitorremediazioa lurzoru kutsatuen kudeaketa iraunkorrerako estrategia gisa","volume":"35","author":"G\u00f3mez-Sagasti","year":"2019","journal-title":"Ekaia"},{"key":"10.1016\/j.cbpc.2022.109276_bb0075","first-page":"3","article-title":"Heavy metal binding by organic substance in sewage sludge of various origin","volume":"9","author":"Gondek","year":"2006","journal-title":"Environ. Dev."},{"key":"10.1016\/j.cbpc.2022.109276_bb0080","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.chemosphere.2014.05.032","article-title":"The effect of low-temperature transformation of mixtures of sewage sludge and plant material son content, leachability and toxicity of heavy metals","volume":"117","author":"Gondek","year":"2014","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109276_bb9000","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.scitotenv.2011.09.037","article-title":"Effects of different gamma exposure regimes on reproduction in the earthworm Eisenia fetida (Oligochaeta)","volume":"412\u2013413","author":"Hertel-Aas","year":"2011","journal-title":"Science of The Total Environment"},{"issue":"8","key":"10.1016\/j.cbpc.2022.109276_bb9010","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1016\/j.ecoenv.2010.07.001","article-title":"Metal-specific effects on metallothionein gene induction and riboflavin content in coelomocytes of Allolobophora chlorotica","volume":"73","author":"H.","year":"2010","journal-title":"Ecotoxicology and Environmental Safety"},{"issue":"3","key":"10.1016\/j.cbpc.2022.109276_bb0100","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1002\/etc.2489","article-title":"Seed germination, root elongation, root-tip mitosis, and micronucleus Induction of five crop plants exposed to chromium in fluvo-aquic soil","volume":"33","author":"Hou","year":"2013","journal-title":"Environ. Toxicol. Chem."},{"issue":"I. Disposiciones generales","key":"10.1016\/j.cbpc.2022.109276_bb9020","first-page":"62274","article-title":"Ley 4\/2015, de 25 de junio, para la prevenci\u00f3n y correcci\u00f3n de la contaminaci\u00f3n del suelo.","author":"IHOBE","year":"2015","journal-title":"BOE"},{"issue":"7","key":"10.1016\/j.cbpc.2022.109276_bb0110","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1007\/s10646-014-1275-x","article-title":"Optimization of NRU assay in primary cultures of Eisenia fetida for metal toxicity assessment","volume":"23","author":"Irizar","year":"2014","journal-title":"Ecotoxicology"},{"key":"10.1016\/j.cbpc.2022.109276_bb0115","first-page":"42","article-title":"Zonation in the digestive tract of Eisenia fetida: implications in biomarker measurements for toxicity assessment","volume":"160","author":"Irizar","year":"2014","journal-title":"Comp.Biochem.Physiol.C"},{"key":"10.1016\/j.cbpc.2022.109276_bb0120","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s00244-014-0060-4","article-title":"Effects of soil organic matter content on cadmium toxicity in Eisenia fetida: implications for the use of biomarkers and standard toxicity tests","volume":"68","author":"Irizar","year":"2015","journal-title":"Arch. Environ. Contam. Toxicol."},{"issue":"5","key":"10.1016\/j.cbpc.2022.109276_bb0125","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1007\/s10646-015-1441-9","article-title":"Establishment of toxicity thresholds in subpopulations of coelomocytes (amoebocytes vs. eleocytes) of Eisenia fetida exposed in vitro to a variety of metals: implications for biomarker measurements","volume":"24","author":"Irizar","year":"2015","journal-title":"Ecotoxicology"},{"key":"10.1016\/j.cbpc.2022.109276_bb0135","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.geoderma.2007.10.002","article-title":"Effect of aging process on the fractionation of heavy metals in some calcareous soils of Iran","volume":"143","author":"Jalali","year":"2008","journal-title":"Geoderma"},{"key":"10.1016\/j.cbpc.2022.109276_bb0140","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/0147-6513(84)90029-0","article-title":"On the dynamics of chemically stressed populations: the deduction of population consequences from effects on individuals","volume":"8","author":"Kooijman","year":"1984","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.cbpc.2022.109276_bb0145","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/j.jhazmat.2015.08.022","article-title":"Feasibility of using humic substances from compost to remove heavy metals (Cd, Cu, Ni, Pb, Zn) from contaminated soil aged for different periods of time","volume":"300","author":"Kulikowska","year":"2015","journal-title":"J. Hazard. Mater."},{"issue":"1","key":"10.1016\/j.cbpc.2022.109276_bb0150","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1021\/acs.est.5b03413","article-title":"Earthworm uptake routes and rates of ionic Zn and ZnO nanoparticles at realistic concentrations, traced using stable isotope labelling","volume":"50","author":"Laycock","year":"2016","journal-title":"Environ. Sci. Technol."},{"issue":"2008","key":"10.1016\/j.cbpc.2022.109276_bb0155","first-page":"219","article-title":"Growth and reproduction of the earthworm Eisenia fetida after exposure to leachate from wood preservatives","volume":"69","author":"Leduc","year":"2007","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.cbpc.2022.109276_bb0160","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/j.scitotenv.2018.07.380","article-title":"Evaluation of combined toxicity of Siduron and cadmium on earthworm (Eisenia fetida) using Biomarker Response Index","volume":"646","author":"Li","year":"2019","journal-title":"Sci. Tot. Envi."},{"key":"10.1016\/j.cbpc.2022.109276_bb9030","series-title":"Overview of the Standard Methods for Soil Ecotoxicology Testing","author":"Lopes Alves","year":"2016"},{"key":"10.1016\/j.cbpc.2022.109276_bb0165","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1007\/BF00203907","article-title":"Tissue-level biomarkers and biological effect of mercury on sentinel slugs, Arion ater","volume":"31","author":"Marig\u00f3mez","year":"1996","journal-title":"Arch.Environ. Contam. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109276_bb0170","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1002\/etc.5620200112","article-title":"Cd, Cu, Pb and Zn coprecipitates in Fe oxide formed at different pH: aging effects on metal solubility and extractability by citrate","volume":"20","author":"Martinez","year":"2001","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2022.109276_bb0175","series-title":"The Study of Pollutants in Ecosystems","article-title":"Ecotoxicology","author":"Moriarty","year":"1983"},{"key":"10.1016\/j.cbpc.2022.109276_bb9040","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.ecoenv.2018.12.047","article-title":"Evaluation of the phytotoxicity of coal ash on lettuce (Lactuca sativa L.) germination, growth and metal uptake","volume":"170","author":"Mtisi","year":"2019","journal-title":"Ecotox. Environ. Saf."},{"key":"10.1016\/j.cbpc.2022.109276_bb0185","series-title":"Terrestrial Plant: Growth Test OECD. Guideline for Chemicals, No., 208","author":"OECD","year":"1984"},{"key":"10.1016\/j.cbpc.2022.109276_bb0190","series-title":"Terrestrial Plant Test: 208: Seedling Emergence And Seedling Growth Test","article-title":"Guideline for the testing of chemicals. Proposal for updating guideline 208","author":"OECD","year":"2003"},{"key":"10.1016\/j.cbpc.2022.109276_bb9050","article-title":"Earthworm ReproductionTests-222","author":"OECD","year":"2016","journal-title":"OECD Guideline for Testing of Chemicals, Section 2"},{"key":"10.1016\/j.cbpc.2022.109276_bb0200","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.envpol.2005.09.023","article-title":"Heavy metals affect the coelomocyte-bacteria balance in earthworms: environmental interactions between abiotic and biotic stressors","volume":"142","author":"Olchawa","year":"2006","journal-title":"Environ. Pollut."},{"issue":"1","key":"10.1016\/j.cbpc.2022.109276_bb9070","doi-asserted-by":"crossref","first-page":"s116","DOI":"10.1016\/j.ejsobi.2007.08.050","article-title":"Flow cytometric measurement of neutral red accumulation in earthworm coelomocytes: Novel assay for studies on heavy metal exposure","volume":"43","author":"Plytycz","year":"2007","journal-title":"European Journal of Soil Biology"},{"key":"10.1016\/j.cbpc.2022.109276_bb0205","doi-asserted-by":"crossref","first-page":"3042","DOI":"10.1016\/j.envpol.2009.05.046","article-title":"Riboflavin content of coelomocytes in earthworm (Dendrodrilus rubidus) field populations as a molecular biomarker of soil metal pollution","volume":"157","author":"Plytycz","year":"2009","journal-title":"Environ. Pollut."},{"issue":"2","key":"10.1016\/j.cbpc.2022.109276_bb9060","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.chemosphere.2010.06.056","article-title":"Riboflavin mobilization from eleocyte stores in the earthworm Dendrodrilus rubidus inhabitating aerially-contaminated Ni smelter soil","volume":"81","author":"Plytycz","year":"2010","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109276_bb0210","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1007\/s10669-008-9171-7","article-title":"Converting wasteland into wonderland by earthworms\u2014a low-cost nature's technology for soil remediation: a case study of vermiremediation of PAHs contaminated soil","volume":"28","author":"Sinha","year":"2008","journal-title":"Environmentalist"},{"key":"10.1016\/j.cbpc.2022.109276_bb0215","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1002\/etc.5620170621","article-title":"Effects of soil type, pre percolation, and ageing on bioaccumulation and toxicity of zinc for the springtail Folsomia candida","volume":"17","author":"Smit","year":"1998","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2022.109276_bb0220","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1897\/08-592.1","article-title":"Toxicity of trace metals in soils affected by soil type and aging after contamination: using calibrated bioavailability models to set ecological soil standards","volume":"28","author":"Smolders","year":"2009","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2022.109276_bb0225","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1007\/BF00116481","article-title":"Extrapolation of the laboratory- based OECD earthworm toxicity test to metal-contaminated field sites","volume":"4","author":"Spurgeon","year":"1995","journal-title":"Ecotoxicology"},{"key":"10.1016\/j.cbpc.2022.109276_bb0230","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0269-7491(94)90094-9","article-title":"Effects of cadmium, copper, lead and zinc on growth, reproduction and survival of the earthworm Eisenia fetida (Savigny): assessing the environmental impact of point-source metal contamination in terrestrial ecosystems","volume":"84","author":"Spurgeon","year":"1994","journal-title":"Environ. Pollut."},{"issue":"766","key":"10.1016\/j.cbpc.2022.109276_bb0235","article-title":"Application of in situ bioremediation strategies in soils amended with sewage sludges","volume":"20","author":"Urionabarrenetxea","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109276_bb9100","first-page":"108735","article-title":"Effects of elevated temperatures and cadmium exposure on stress biomarkers at different biological complexity levels in Eisenia fetida earthworms","volume":"231","author":"Urionabarrenetxea","year":"2020","journal-title":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"},{"key":"10.1016\/j.cbpc.2022.109276_bb0240","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.chemosphere.2017.09.122","article-title":"Bisphenol A in artificial soil: effects on growth, reproduction and immunity in earthworms","volume":"190","author":"Verd\u00fa","year":"2018","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109276_bb0245","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1016\/j.chemosphere.2012.09.081","article-title":"Metal toxicity and biodiversity in serpentine soils: application of bioassay tests and microarthropod index","volume":"90","author":"Visioli","year":"2013","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109276_bb0250","doi-asserted-by":"crossref","first-page":"8979","DOI":"10.1007\/s11356-014-3577-1","article-title":"Influence of ageing on lead bioavailability in soils: a swine study","volume":"22","author":"Wijayawardena","year":"2015","journal-title":"Environ. Sci. Pollut. Res."}],"container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045622000114?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045622000114?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:28:30Z","timestamp":1761517710000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1532045622000114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5]]},"references-count":50,"alternative-id":["S1532045622000114"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpc.2022.109276","relation":{},"ISSN":["1532-0456"],"issn-type":[{"value":"1532-0456","type":"print"}],"subject":[],"published":{"date-parts":[[2022,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Impacts of sewage sludges deposition on agricultural soils: Effects upon model soil organisms","name":"articletitle","label":"Article Title"},{"value":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbpc.2022.109276","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"109276"}}