{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T22:00:10Z","timestamp":1791583210821,"version":"4.3.4"},"reference-count":98,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T00:00:00Z","timestamp":1665619200000},"content-version":"vor","delay-in-days":73,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Biochemistry and Physiology Part C: Toxicology &amp; Pharmacology"],"published-print":{"date-parts":[[2022,8]]},"DOI":"10.1016\/j.cbpc.2022.109363","type":"journal-article","created":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T07:49:44Z","timestamp":1651650584000},"page":"109363","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":80,"special_numbering":"C","title":["Oxidative stress, apoptosis and serotonergic system changes in zebrafish (Danio rerio) gills after long-term exposure to microplastics and copper"],"prefix":"10.1016","volume":"258","author":[{"given":"D\u00e9rcia","family":"Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Luzio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds","family":"F\u00e9lix","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Bellas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra M.","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cbpc.2022.109363_bb0005","doi-asserted-by":"crossref","first-page":"2050","DOI":"10.1016\/j.marpolbul.2010.07.014","article-title":"Association of metals with plastic production pellets in the marine environment","volume":"60","author":"Ashton","year":"2010","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.cbpc.2022.109363_bb0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2019.07.066","article-title":"Evaluation of single and combined effects of cadmium and micro-plastic particles on biochemical and immunological parameters of common carp (Cyprinus carpio)","volume":"236","author":"Banaee","year":"2019","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109363_bb0015","doi-asserted-by":"crossref","first-page":"15655","DOI":"10.1038\/s41598-018-34125-z","article-title":"Microplastics increase mercury bioconcentration in gills and bioaccumulation in the liver, and cause oxidative stress and damage in Dicentrarchus labrax juveniles","volume":"8","author":"Barboza","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cbpc.2022.109363_bb0020","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.aquatox.2017.12.008","article-title":"Microplastics cause neurotoxicity, oxidative damage and energy-related changes and interact with the bioaccumulation of mercury in the European seabass, Dicentrarchus labrax (Linnaeus, 1758)","volume":"195","author":"Barboza","year":"2018","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0025","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1016\/j.envpol.2017.12.082","article-title":"Single and combined effects of microplastics and mercury on juveniles of the European seabass (Dicentrarchus labrax): changes in behavioural responses and reduction of swimming velocity and resistance time","volume":"236","author":"Barboza","year":"2018","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.cbpc.2022.109363_bb0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2019.134625","article-title":"Microplastics in wild fish from North East Atlantic Ocean and its potential for causing neurotoxic effects, lipid oxidative damage, and human health risks associated with ingestion exposure","volume":"717","author":"Barboza","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0035","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1002\/etc.5620210629","article-title":"Integrated biomarker response: a useful tool for ecological risk assessment","volume":"21","author":"Beliaeff","year":"2002","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2022.109363_bb0040","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.aquatox.2019.02.022","article-title":"Combined effects of nanoplastics and copper on the freshwater alga Raphidocelis subcapitata","volume":"210","author":"Bellingeri","year":"2019","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0045","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1038\/sj.cdd.4400525","article-title":"Apoptosis without caspases: an inefficient molecular guillotine?","volume":"6","author":"Borner","year":"1999","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0050","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.cbpc.2022.109363_bb0055","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2021.144976","article-title":"Long-term exposure to virgin and seawater exposed microplastic enriched-diet causes liver oxidative stress and inflammation in gilthead seabream Sparus aurata, Linnaeus 1758","volume":"767","author":"Cap\u00f3","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0060","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.biochi.2014.06.020","article-title":"Serotonin activates cell survival and apoptotic death responses in cultured epithelial thyroid cells","volume":"105","author":"Cerulo","year":"2014","journal-title":"Biochimie"},{"key":"10.1016\/j.cbpc.2022.109363_bb0065","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquatox.2020.105521","article-title":"Microplastics lead to hyperactive swimming behaviour in adult zebrafish","volume":"224","author":"Chen","year":"2020","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2021.126482","article-title":"Chronic exposure to UV-aged microplastics induces neurotoxicity by affecting dopamine, glutamate, and serotonin neurotransmission in Caenorhabditis elegans","volume":"419","author":"Chen","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.cbpc.2022.109363_bb0075","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.141280","article-title":"Combined effects of mulch film-derived microplastics and atrazine on oxidative stress and gene expression in earthworm (Eisenia fetida)","volume":"746","author":"Cheng","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0080","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.marpolbul.2018.02.039","article-title":"Toxicological effects of irregularly shaped and spherical microplastics in a marine teleost, the sheepshead minnow (Cyprinodon variegatus)","volume":"129","author":"Choi","year":"2018","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.cbpc.2022.109363_bb0085","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1080\/10934529.2018.1474577","article-title":"Assessment of Douro and Ave River (Portugal) lower basin water quality focusing on physicochemical and trace element spatiotemporal changes","volume":"53","author":"Couto","year":"2018","journal-title":"J. Environ. Sci. Health Toxicol. Hazard Subst Environ. Eng."},{"key":"10.1016\/j.cbpc.2022.109363_bb0090","doi-asserted-by":"crossref","first-page":"2448","DOI":"10.1007\/s11356-013-2169-9","article-title":"The integrated biomarker response revisited: optimization to avoid misuse","volume":"21","author":"Devin","year":"2014","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.cbpc.2022.109363_bb0095","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/j.wasman.2018.06.037","article-title":"Characterization of plastic debris and association of metals with microplastics in coastline sediment along the Persian Gulf","volume":"78","author":"Dobaradaran","year":"2018","journal-title":"Waste Manag."},{"key":"10.1016\/j.cbpc.2022.109363_bb0100","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12302-015-0069-y","article-title":"Microplastics in the aquatic and terrestrial environment: sources (with a specific focus on personal care products), fate and effects","volume":"28","author":"Duis","year":"2016","journal-title":"Environ. Sci. Eur."},{"key":"10.1016\/j.cbpc.2022.109363_bb0105","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1080\/01926230701320337","article-title":"Apoptosis: a review of programmed cell death","volume":"35","author":"Elmore","year":"2007","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0110","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.fsi.2019.10.072","article-title":"Dietary administration of PVC and PE microplastics produces histological damage, oxidative stress and immunoregulation in European sea bass (Dicentrarchus labrax L.)","volume":"95","author":"Espinosa","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0115","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1289\/ehp.877147","article-title":"The fish gill: site of action and model for toxic effects of environmental pollutants","volume":"71","author":"Evans","year":"1987","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.cbpc.2022.109363_bb0120","doi-asserted-by":"crossref","first-page":"61254","DOI":"10.1039\/C6RA08298J","article-title":"Ketamine-induced oxidative stress at different developmental stages of zebrafish (Danio rerio) embryos","volume":"6","author":"F\u00e9lix","year":"2016","journal-title":"RSC Adv."},{"key":"10.1016\/j.cbpc.2022.109363_bb0125","doi-asserted-by":"crossref","first-page":"1988","DOI":"10.1016\/j.marpolbul.2010.07.030","article-title":"Organic pollutants in microplastics from two beaches of the Portuguese coast","volume":"60","author":"Frias","year":"2010","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.cbpc.2022.109363_bb0130","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.scitotenv.2019.05.161","article-title":"Impacts of dietary exposure to different sized polystyrene microplastics alone and with sorbed benzo[a]pyrene on biomarkers and whole organism responses in mussels Mytilus galloprovincialis","volume":"684","author":"Gonz\u00e1lez-Soto","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0135","series-title":"Fish Physiology, Homeostasis And Toxicology of Essential Metals","first-page":"53","article-title":"2 - copper","author":"Grosell","year":"2011"},{"key":"10.1016\/j.cbpc.2022.109363_bb0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2020.122909","article-title":"Microplastics aggravate the adverse effects of BDE-47 on physiological and defense performance in mussels","volume":"398","author":"Gu","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.cbpc.2022.109363_bb0145","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/j.scitotenv.2017.12.020","article-title":"Uptake and effects of the antimicrobial florfenicol, microplastics and their mixtures on freshwater exotic invasive bivalve Corbicula fluminea","volume":"622\u2013623","author":"Guilhermino","year":"2018","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2020.124833","article-title":"Toxic effects of naturally-aged microplastics on zebrafish juveniles: a more realistic approach to plastic pollution in freshwater ecosystems","volume":"407","author":"Guimar\u00e3es","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.cbpc.2022.109363_bb0155","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0003-9861(92)90507-S","article-title":"Direct evidence for inhibition of free radical formation from Cu(I) and hydrogen peroxide by glutathione and other potential ligands using the EPR spin-trapping technique","volume":"295","author":"Hanna","year":"1992","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.cbpc.2022.109363_bb0160","doi-asserted-by":"crossref","first-page":"R19","DOI":"10.1186\/gb-2007-8-2-r19","article-title":"qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data","volume":"8","author":"Hellemans","year":"2007","journal-title":"Genome Biol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0165","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3390\/toxics4030019","article-title":"Zebrafish get connected: investigating neurotransmission targets and alterations in chemical toxicity","volume":"4","author":"Horzmann","year":"2016","journal-title":"Toxics"},{"key":"10.1016\/j.cbpc.2022.109363_bb0170","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2021.112012","article-title":"Polystyrene microplastics lead to pyroptosis and apoptosis of ovarian granulosa cells via NLRP3\/Caspase-1 signaling pathway in rats","volume":"212","author":"Hou","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.cbpc.2022.109363_bb0175","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2020.123044","article-title":"Integrated response of growth, antioxidant defense and isotopic composition to microplastics in juvenile guppy (Poecilia reticulata)","volume":"399","author":"Huang","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.cbpc.2022.109363_bb0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2021.126830","article-title":"Micro\/nano-plastics cause neurobehavioral toxicity in discus fish (Symphysodon aequifasciatus): insight from brain-gut-microbiota axis","volume":"421","author":"Huang","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.cbpc.2022.109363_bb0185","doi-asserted-by":"crossref","DOI":"10.1007\/s00244-022-00924-9","article-title":"Effect of early-life exposure of polystyrene microplastics on behavior and DNA methylation in later life stage of zebrafish","author":"Im","year":"2022","journal-title":"Arch. Environ. Contam.Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0190","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1021\/tx020022u","article-title":"Contribution of glutathione and metallothioneins to protection against copper toxicity and redox cycling: quantitative analysis using MT+\/+ and MT\u2212\/\u2212 mouse lung fibroblast cells","volume":"15","author":"Jiang","year":"2002","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.marpolbul.2019.110510","article-title":"Effects of microplastics and attached heavy metals on growth, immunity, and heavy metal accumulation in the yellow seahorse,Hippocampus kuda Bleeker","volume":"149","author":"Jinhui","year":"2019","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.cbpc.2022.109363_bb0200","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2020.123980","article-title":"Toxicity of polystyrene microplastics on juvenile Oncorhynchus mykiss (rainbow trout) after individual and combined exposure with chlorpyrifos","volume":"403","author":"Karbalaei","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.cbpc.2022.109363_bb0205","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3109\/10408449309104073","article-title":"Free radicals as mediators of tissue injury and disease","volume":"23","author":"Kehrer","year":"1993","journal-title":"Crit. Rev. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0210","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.cbi.2004.12.004","article-title":"Possible mechanisms underlying copper-induced damage in biological membranes leading to cellular toxicity","volume":"151","author":"Letelier","year":"2005","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.cbpc.2022.109363_bb0215","series-title":"General, Applied And Systems Toxicology","article-title":"Glutathione and glutathione-S-transferase in detoxification mechanisms","author":"Li","year":"2011"},{"key":"10.1016\/j.cbpc.2022.109363_bb0220","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2021.126025","article-title":"Polystyrene microplastics trigger hepatocyte apoptosis and abnormal glycolytic flux via ROS-driven calcium overload","volume":"417","author":"Li","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.cbpc.2022.109363_bb0225","doi-asserted-by":"crossref","first-page":"15775","DOI":"10.1038\/s41598-019-52292-5","article-title":"Microplastics induce transcriptional changes, immune response and behavioral alterations in adult zebrafish","volume":"9","author":"Limonta","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cbpc.2022.109363_bb0230","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.chemosphere.2018.03.145","article-title":"Influence of microplastics on the accumulation and chronic toxic effects of cadmium in zebrafish (Danio rerio)","volume":"202","author":"Lu","year":"2018","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109363_bb0235","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.aquatox.2015.04.018","article-title":"Does the presence of microplastics influence the acute toxicity of chromium(VI) to early juveniles of the common goby (Pomatoschistus microps)? A study with juveniles from two wild estuarine populations","volume":"164","author":"Lu\u00eds","year":"2015","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0240","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1007\/s10695-015-0171-5","article-title":"Contaminant-induced oxidative stress in fish: a mechanistic approach","volume":"42","author":"Lushchak","year":"2016","journal-title":"Fish Physiol. Biochem."},{"key":"10.1016\/j.cbpc.2022.109363_bb0245","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.aquatox.2012.12.018","article-title":"Copper induced upregulation of apoptosis related genes in zebrafish (Danio rerio) gill","volume":"128\u2013129","author":"Luzio","year":"2013","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0250","doi-asserted-by":"crossref","DOI":"10.1016\/j.etap.2021.103674","article-title":"Copper impair autophagy on zebrafish (Danio rerio) gill epithelium","volume":"86","author":"Luzio","year":"2021","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0255","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2020.114089","article-title":"Microplastics in aquatic environments: toxicity to trigger ecological consequences","volume":"261","author":"Ma","year":"2020","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.cbpc.2022.109363_bb0260","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1007\/s00128-016-1918-0","article-title":"Assessment of heavy metal contamination and calculation of its pollution index for Ugljesnica River,Serbia","volume":"97","author":"Milivojevic","year":"2016","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0265","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph16162857","article-title":"Neurotoxicity, behavior, and lethal effects of cadmium, microplastics, and their mixtures on Pomatoschistus microps juveniles from two wild populations exposed under laboratory conditions-implications to environmental and human risk assessment","volume":"16","author":"Miranda","year":"2019","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.cbpc.2022.109363_bb0270","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1111\/j.1095-8649.2008.02009.x","article-title":"Quantitative histopathology of Oreochromis niloticus gills after copper exposure","volume":"73","author":"Monteiro","year":"2008","journal-title":"J. Fish Biol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0275","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.aquatox.2009.07.008","article-title":"Copper toxicity in gills of the teleost fish, Oreochromis niloticus: effects in apoptosis induction and cell proliferation","volume":"94","author":"Monteiro","year":"2009","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0280","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0191759","article-title":"Macro and micro plastics sorb and desorb metals and act as a point source of trace metals to coastal ecosystems","volume":"13","author":"Munier","year":"2018","journal-title":"PLoS ONE"},{"key":"10.1016\/j.cbpc.2022.109363_bb0285","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1016\/j.scitotenv.2018.04.180","article-title":"Environmental mixtures of nanomaterials and chemicals: the Trojan-horse phenomenon and its relevance for ecotoxicity","volume":"635","author":"Naasz","year":"2018","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0290","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s00244-020-00729-8","article-title":"Effects of microplastics associated with triclosan on the oyster Crassostrea brasiliana: an integrated biomarker approach","volume":"79","author":"Nobre","year":"2020","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0295","first-page":"5","article-title":"Ecotoxicological effects of chemical contaminants adsorbed to microplastics in the clam Scrobicularia plana","author":"O\u2019Donovan","year":"2018","journal-title":"Front. Mar.Sci."},{"key":"10.1016\/j.cbpc.2022.109363_bb0300","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.ecoenv.2007.10.034","article-title":"European eel (Anguilla anguilla L.) metallothionein, endocrine, metabolic and genotoxic responses to copper exposure","volume":"70","author":"Oliveira","year":"2008","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.cbpc.2022.109363_bb0305","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.ecoenv.2018.07.062","article-title":"Effects of microplastics and mercury in the freshwater bivalve Corbicula fluminea (M\u00fcller, 1774): filtration rate, biochemical biomarkers and mercury bioconcentration","volume":"164","author":"Oliveira","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.cbpc.2022.109363_bb0310","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S1382-6689(02)00126-6","article-title":"Fish bioaccumulation and biomarkers in environmental risk assessment: a review","volume":"13","author":"van der Oost","year":"2003","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0315","doi-asserted-by":"crossref","DOI":"10.1016\/j.resp.2020.103594","article-title":"Disruption of tph1 genes demonstrates the importance of serotonin in regulating ventilation in larval zebrafish (Danio rerio)","volume":"285","author":"Pan","year":"2021","journal-title":"Respir. Physiol. Neurobiol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0320","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3390\/w13040394","article-title":"Multi-biomarker responses of asian clam Corbicula fluminea (Bivalvia, Corbiculidea) to cadmium and microplastics pollutants","volume":"13","author":"Parra","year":"2021","journal-title":"Water"},{"key":"10.1016\/j.cbpc.2022.109363_bb0325","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.140706","article-title":"Microplastic and heavy metal distributions in an Indian coral reef ecosystem","volume":"744","author":"Patterson","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0330","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2020.127843","article-title":"Copper release from waste rocks in an abandoned mine (NE, Brazil) and its impacts on ecosystem environmental quality","volume":"262","author":"Perlatti","year":"2021","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109363_bb0335","doi-asserted-by":"crossref","DOI":"10.1016\/j.marpolbul.2020.111380","article-title":"Concentration and adsorption of Pb and Cu in microplastics: case study in aquatic environment","volume":"158","author":"Purwiyanto","year":"2020","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.cbpc.2022.109363_bb0340","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2020.128161","article-title":"Exposure to polystyrene microplastics impairs gonads of zebrafish (Danio rerio)","volume":"263","author":"Qiang","year":"2021","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109363_bb0345","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.scitotenv.2019.01.245","article-title":"Microplastics induce intestinal inflammation, oxidative stress, and disorders of metabolome and microbiome in zebrafish","volume":"662","author":"Qiao","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0350","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.scitotenv.2019.05.163","article-title":"Combined effects of polystyrene microplastics and natural organic matter on the accumulation and toxicity of copper in zebrafish","volume":"682","author":"Qiao","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0355","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.marpolbul.2018.09.048","article-title":"Sorption and desorption of selected pharmaceuticals by polyethylene microplastics","volume":"136","author":"Razanajatovo","year":"2018","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.cbpc.2022.109363_bb0360","doi-asserted-by":"crossref","first-page":"2977","DOI":"10.1016\/j.bbamcr.2016.09.012","article-title":"Activation of apoptosis signalling pathways by reactive oxygen species","volume":"1863","author":"Redza-Dutordoir","year":"2016","journal-title":"<sb:contribution><sb:title>Biochim.<\/sb:title><\/sb:contribution><sb:host><sb:issue><sb:series><sb:title>Biophys. Acta BBA Mol. Cell Res.<\/sb:title><\/sb:series><\/sb:issue><\/sb:host>"},{"key":"10.1016\/j.cbpc.2022.109363_bb0365","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3389\/fenvs.2019.00033","article-title":"Tissue-specific biomarker responses in the blue mussel Mytilus spp. exposed to a mixture of microplastics at environmentally relevant concentrations","volume":"7","author":"Revel","year":"2019","journal-title":"Front. Environ. Sci."},{"key":"10.1016\/j.cbpc.2022.109363_bb0370","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpc.2021.109106","article-title":"Short communication: effects of acute copper exposure on ionic regulation of the freshwater crab Aegla castro","volume":"248","author":"Rosa","year":"2021","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0375","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2020.127748","article-title":"Toxicological effects induced on early life stages of zebrafish (Danio rerio) after an acute exposure to microplastics alone or co-exposed with copper","volume":"261","author":"Santos","year":"2020","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109363_bb0380","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2021.130262","article-title":"Single and combined acute and subchronic toxic effects of microplastics and copper in zebrafish (Danio rerio) early life stages","volume":"277","author":"Santos","year":"2021","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109363_bb0385","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquatox.2021.105814","article-title":"Microplastics alone or co-exposed with copper induce neurotoxicity and behavioral alterations on zebrafish larvae after a subchronic exposure","volume":"235","author":"Santos","year":"2021","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0390","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.3390\/ijms21041410","article-title":"Nanoplastics cause neurobehavioral impairments, reproductive and oxidative damages, and biomarker responses in zebrafish: throwing up alarms of wide spread health risk of exposure","volume":"21","author":"Sarasamma","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.cbpc.2022.109363_bb0395","doi-asserted-by":"crossref","DOI":"10.1016\/j.marpolbul.2020.111747","article-title":"Microplastics pollution with heavy metals in the aquaculture zone of the Chao Phraya River Estuary,Thailand","volume":"161","author":"Ta","year":"2020","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.cbpc.2022.109363_bb0400","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1098\/rstb.2008.0284","article-title":"Transport and release of chemicals from plastics to the environment and to wildlife","volume":"364","author":"Teuten","year":"2009","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"10.1016\/j.cbpc.2022.109363_bb0405","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1071\/EN14143","article-title":"Adsorption of trace metals by microplastic pellets in fresh water","volume":"12","author":"Turner","year":"2015","journal-title":"Environ. Chem."},{"key":"10.1016\/j.cbpc.2022.109363_bb0410","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbi.2021.109550","article-title":"Polystyrene microplastics induce apoptosis via ROS-mediated p53 signaling pathway in zebrafish","volume":"345","author":"Umamaheswari","year":"2021","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.cbpc.2022.109363_bb0415","series-title":"Aquatic Life Ambient Freshwater Quality Criteria-copper, EPA-822-R-07-001","author":"USEPA","year":"2007"},{"key":"10.1016\/j.cbpc.2022.109363_bb0420","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2021.130979","article-title":"Ingestion of microplastics and its potential for causing structural alterations and oxidative stress in indian green mussel Perna viridis \u2013 a multiple biomarker approach","volume":"283","author":"Vasanthi","year":"2021","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109363_bb0425","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0141-1136(96)00103-1","article-title":"A simple spectrophotometric method for metallothionein evaluation in marine organisms: an application to Mediterranean and Antarctic molluscs","volume":"44","author":"Viarengo","year":"1997","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.cbpc.2022.109363_bb0430","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1016\/j.chemosphere.2009.06.005","article-title":"Acute effects of copper and mercury on the estuarine fish Pomatoschistus microps: linking biomarkers to behaviour","volume":"76","author":"Vieira","year":"2009","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109363_bb0435","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmars.2014.00034","article-title":"Characteristics, functions, and applications of metallothionein in aquatic vertebrates","volume":"1","author":"Wang","year":"2014","journal-title":"Front. Mar. Sci."},{"key":"10.1016\/j.cbpc.2022.109363_bb0440","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.chemosphere.2016.12.074","article-title":"Microplastics in the surface sediments from the Beijiang River littoral zone: composition, abundance, surface textures and interaction with heavy metals","volume":"171","author":"Wang","year":"2017","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109363_bb0445","doi-asserted-by":"crossref","first-page":"5364","DOI":"10.1021\/acs.est.6b01187","article-title":"Effect of microplastic on the gills of the shore crab Carcinus maenas","volume":"50","author":"Watts","year":"2016","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0450","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.envpol.2018.09.029","article-title":"Single and combined effects of microplastics and cadmium on the cadmium accumulation, antioxidant defence and innate immunity of the discus fish (Symphysodon aequifasciatus)","volume":"243","author":"Wen","year":"2018","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.cbpc.2022.109363_bb0455","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.cbpc.2019.04.013","article-title":"Single and combined exposures of waterborne Cu and Cd induced oxidative stress responses and tissue injury in female rare minnow (Gobiocypris rarus)","volume":"222","author":"Wu","year":"2019","journal-title":"Comp. Biochem. Physiol.C Toxicol. Pharmacol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0460","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2019.136479","article-title":"Polyvinyl chloride microplastics induce growth inhibition and oxidative stress in Cyprinus carpio var. larvae","volume":"716","author":"Xia","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0465","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2020.122795","article-title":"Individual and combined toxicogenetic effects of microplastics and heavy metals (Cd, Pb, and Zn) perturb gut microbiota homeostasis and gonadal development in marine medaka (Oryzias melastigma)","volume":"397","author":"Yan","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.cbpc.2022.109363_bb0470","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.aquatox.2018.04.015","article-title":"Accumulation of polystyrene microplastics in juvenile Eriocheir sinensis and oxidative stress effects in the liver","volume":"200","author":"Yu","year":"2018","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2022.109363_bb0475","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2020.126067","article-title":"Enhanced adsorption of tetrabromobisphenol a (TBBPA) on cosmetic-derived plastic microbeads and combined effects on zebrafish","volume":"248","author":"Yu","year":"2020","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2022.109363_bb0480","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.141254","article-title":"Toxicological effects of microplastics and heavy metals on the Daphnia magna","volume":"746","author":"Yuan","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.cbpc.2022.109363_bb0485","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s00441-016-2531-6","article-title":"Distribution and morphology of cholinergic cells in the branchial epithelium of zebrafish (Danio rerio)","volume":"367","author":"Zachar","year":"2017","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.cbpc.2022.109363_bb0490","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2020.126064","article-title":"Adsorption of three bivalent metals by four chemical distinct microplastics","volume":"248","author":"Zou","year":"2020","journal-title":"Chemosphere"}],"container-title":["Comparative Biochemistry and Physiology Part C: Toxicology &amp; Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045622000989?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045622000989?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:29:24Z","timestamp":1761517764000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1532045622000989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8]]},"references-count":98,"alternative-id":["S1532045622000989"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpc.2022.109363","relation":{},"ISSN":["1532-0456"],"issn-type":[{"value":"1532-0456","type":"print"}],"subject":[],"published":{"date-parts":[[2022,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Oxidative stress, apoptosis and serotonergic system changes in zebrafish (Danio rerio) gills after long-term exposure to microplastics and copper","name":"articletitle","label":"Article Title"},{"value":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbpc.2022.109363","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"109363"}}