{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T13:42:53Z","timestamp":1780926173517,"version":"3.54.1"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100002564","name":"Alternatives Research and Development Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002564","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007069","name":"Oklahoma State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"published-print":{"date-parts":[[2023,8]]},"DOI":"10.1016\/j.cbpc.2023.109638","type":"journal-article","created":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T15:51:09Z","timestamp":1683215469000},"page":"109638","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Characterization of RTgill-W1 cells epithelial development on transwell inserts: Evaluation of osmotic and toxic challenges"],"prefix":"10.1016","volume":"270","author":[{"given":"Justin","family":"Scott","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Minghetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"09","key":"10.1016\/j.cbpc.2023.109638_bb0005","article-title":"Meyler\u2019s side effects of drugs: the international encyclopedia of adverse drug reactions and interactions","volume":"44","author":"Aronson","year":"2007","journal-title":"Choice Rev. Online"},{"issue":"7","key":"10.1016\/j.cbpc.2023.109638_bb0010","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1002\/jbm.820190704","article-title":"Fibronectin binding properties of bacteriologic petri plates and tissue culture dishes","volume":"19","author":"Bentley","year":"1985","journal-title":"J. Biomed. Mater. Res."},{"issue":"15","key":"10.1016\/j.cbpc.2023.109638_bb0015","doi-asserted-by":"crossref","first-page":"9547","DOI":"10.1021\/acs.est.0c02522","article-title":"Mechanisms of pH-dependent uptake of ionizable organic chemicals by fish from Oil Sands process-affected water (OSPW)","volume":"54","author":"Brinkmann","year":"2020","journal-title":"Environ Sci Technol."},{"issue":"1","key":"10.1016\/j.cbpc.2023.109638_bb0020","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/etc.5620180107","article-title":"Effects of altering freshwater chemistry on physiological responses of rainbow trout to silver exposure","volume":"18","author":"Bury","year":"1999","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2023.109638_bb0025","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.semcdb.2015.04.003","article-title":"Claudin switching: physiological plasticity of the tight junction","volume":"42","author":"Capaldo","year":"2015","journal-title":"Semin. Cell Dev. Biol."},{"issue":"5","key":"10.1016\/j.cbpc.2023.109638_bb0030","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s00418-015-1351-1","article-title":"Transepithelial electrical resistance (TEER): a functional parameter to monitor the quality of oviduct epithelial cells cultured on filter supports","volume":"144","author":"Chen","year":"2015","journal-title":"Histochem. Cell Biol."},{"issue":"15","key":"10.1016\/j.cbpc.2023.109638_bb0035","doi-asserted-by":"crossref","first-page":"3727","DOI":"10.1016\/S0043-1354(02)00078-7","article-title":"Applying whole-water samples directly to fish cell cultures in order to evaluate the toxicity of industrial effluent","volume":"36","author":"Dayeh","year":"2002","journal-title":"Water Res."},{"issue":"11","key":"10.1016\/j.cbpc.2023.109638_bb0040","doi-asserted-by":"crossref","first-page":"9496","DOI":"10.1021\/acsami.7b00705","article-title":"Ultrathin alumina membranes as scaffold for epithelial cell culture from the intestine of rainbow trout","volume":"9","author":"Drieschner","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"2","key":"10.1016\/j.cbpc.2023.109638_bb0045","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s002329900521","article-title":"Quantitative determination of gap junctional permeability in the lens cortex","volume":"169","author":"Eckert","year":"1999","journal-title":"J. Membr. Biol."},{"issue":"1","key":"10.1016\/j.cbpc.2023.109638_bb0050","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1006\/taap.1998.8479","article-title":"Potential mechanisms of the enhancement of aldicarb toxicity to japanese medaka, Oryzias latipes, at high salinity","volume":"152","author":"El-Alfy","year":"1998","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.cbpc.2023.109638_bb0055","first-page":"1","article-title":"Repeatability and reproducibility of the RTgill-W1 cell line assay for predicting fish acute toxicity r \u0131 Nov a","author":"Fischer","year":"2019","journal-title":"Toxicol. Sci."},{"issue":"3","key":"10.1016\/j.cbpc.2023.109638_bb0060","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.1021\/acs.est.1c05839","article-title":"Inflammation of gill epithelia in fish causes increased permeation of petrogenic polar organic chemicals via disruption of tight junctions","volume":"56","author":"Fuchylo","year":"2022","journal-title":"Environ. Sci. Technol."},{"issue":"16","key":"10.1016\/j.cbpc.2023.109638_bb0065","doi-asserted-by":"crossref","first-page":"9431","DOI":"10.1021\/acs.est.8b01663","article-title":"Ecotoxicological effects of transformed silver and titanium dioxide nanoparticles in the effluent from a lab-scale wastewater treatment system","volume":"52","author":"Georgantzopoulou","year":"2018","journal-title":"Environ. Sci. Technol."},{"issue":"2","key":"10.1016\/j.cbpc.2023.109638_bb0070","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1039\/C5EN00226E","article-title":"A novel two-compartment barrier model for investigating nanoparticle transport in fish intestinal epithelial cells","volume":"3","author":"Geppert","year":"2016","journal-title":"Environ. Sci. Nano"},{"key":"10.1016\/j.cbpc.2023.109638_bb0075","series-title":"Water Quality \u2014 Determination of Acute Toxicity of Water Samples and Chemicals to a Fish Gill Cell Line (RTgill-W1) ISO 21115;2019","author":"ISO","year":"2019"},{"issue":"6","key":"10.1016\/j.cbpc.2023.109638_bb0080","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1039\/c0em00791a","article-title":"Effects of salinity and organic matter on the partitioning of perfluoroalkyl acid (PFAs) to clay particles","volume":"13","author":"Jeon","year":"2011","journal-title":"J. Environ. Monit."},{"issue":"2","key":"10.1016\/j.cbpc.2023.109638_bb0085","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.chemosphere.2010.06.037","article-title":"Effect of perfluorooctanesulfonate on osmoregulation in marine fish, Sebastes schlegeli, under different salinities","volume":"81","author":"Jeon","year":"2010","journal-title":"Chemosphere"},{"issue":"1","key":"10.1016\/j.cbpc.2023.109638_bb0090","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1086\/physzool.64.1.30158511","article-title":"Environmental effects on fish gill morphology","volume":"64","author":"Laurent","year":"1991","journal-title":"Physiol. Zool."},{"issue":"3\u20134","key":"10.1016\/j.cbpc.2023.109638_bb0095","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s11626-008-9173-2","article-title":"Applications and potential uses of fish gill cell lines: examples with RTgill-W1","volume":"45","author":"Lee","year":"2009","journal-title":"In Vitro Cell. Dev. Biol. Anim."},{"issue":"11","key":"10.1016\/j.cbpc.2023.109638_bb0100","doi-asserted-by":"crossref","first-page":"2637","DOI":"10.1002\/etc.3603","article-title":"Alternative approaches to vertebrate ecotoxicity tests in the 21st century: a review of developments over the last 2 decades and current status","volume":"35","author":"Lillicrap","year":"2016","journal-title":"Environ. Toxicol. Chem."},{"issue":"August","key":"10.1016\/j.cbpc.2023.109638_bb0105","first-page":"1","article-title":"Polarized trout epithelial cells regulate transepithelial electrical resistance, gene expression, and the phosphoproteome in response to viral infection","volume":"11","author":"Mandal","year":"2020","journal-title":"Front. Immunol."},{"issue":"December 2021","key":"10.1016\/j.cbpc.2023.109638_bb0110","article-title":"Influence of TiO2 nanocomposite UV filter surface chemistry and their interactions with organic UV filters on uptake and toxicity toward cultured fish gill cells","volume":"243","author":"Martin","year":"2022","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.cbpc.2023.109638_bb0115","doi-asserted-by":"crossref","DOI":"10.1242\/jeb.220251","article-title":"Cortisol is an osmoregulatory and glucose-regulating hormone in Atlantic sturgeon, a basal ray-finned fish","volume":"223","author":"McCormick","year":"2020","journal-title":"J. Exp. Biol."},{"issue":"6","key":"10.1016\/j.cbpc.2023.109638_bb0120","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1007\/s10565-017-9385-x","article-title":"A fish intestinal epithelial barrier model established from the rainbow trout (Oncorhynchus mykiss) cell line, RTgutGC","volume":"33","author":"Minghetti","year":"2017","journal-title":"Cell Biol. Toxicol."},{"issue":"10","key":"10.1016\/j.cbpc.2023.109638_bb0125","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1080\/17435390.2016.1241908","article-title":"Effect of media composition on bioavailability and toxicity of silver and silver nanoparticles in fish intestinal cells (RTgutGC)","volume":"10","author":"Minghetti","year":"2016","journal-title":"Nanotoxicology"},{"issue":"6","key":"10.1016\/j.cbpc.2023.109638_bb0130","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1039\/C9EN00310J","article-title":"Interference of silver nanoparticles with essential metal homeostasis in a novel enterohepatic fish: in vitro system","volume":"6","author":"Minghetti","year":"2019","journal-title":"Environ. Sci. Nano"},{"key":"10.1016\/j.cbpc.2023.109638_bb0135","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.aquatox.2014.05.019","article-title":"A primary FIsh gill cell system (FIGCS) for environmental monitoring of river waters","volume":"154","author":"Minghetti","year":"2014","journal-title":"Aquat. Toxicol."},{"issue":"3","key":"10.1016\/j.cbpc.2023.109638_bb0140","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1002\/etc.4027","article-title":"Accurate prediction of acute fish toxicity of fragrance chemicals with the RTgill-W1 cell assay","volume":"37","author":"Natsch","year":"2018","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2023.109638_bb0145","series-title":"Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 Cell Line Assay. Guidel Test Chem Sect 2","volume":"249","author":"OECD","year":"2021"},{"issue":"July 2022","key":"10.1016\/j.cbpc.2023.109638_bb0150","article-title":"Role of the luminal composition on intestinal metal toxicity, bioavailability and bioreactivity: an in vitro approach based on the cell line RTgutGC","volume":"256","author":"Oldham","year":"2023","journal-title":"Aquat. Toxicol."},{"issue":"3","key":"10.1016\/j.cbpc.2023.109638_bb0155","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.ecoenv.2006.10.007","article-title":"Effects of anionic surfactant and salinity on the bioenergetics of juveniles of Centropomus parallelus (Poey)","volume":"68","author":"Rocha","year":"2007","journal-title":"Ecotoxicol. Environ. Saf."},{"issue":"9","key":"10.1016\/j.cbpc.2023.109638_bb0160","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1242\/jeb.050062","article-title":"Claudin 28b and F-actin are involved in rainbow trout gill pavement cell tight junction remodeling under osmotic stress","volume":"214","author":"Sandbichler","year":"2011","journal-title":"J. Exp. Biol."},{"issue":"3","key":"10.1016\/j.cbpc.2023.109638_bb0165","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.tox.2006.04.042","article-title":"Proposal to improve vertebrate cell cultures to establish them as substitutes for the regulatory testing of chemicals and effluents using fish","volume":"224","author":"Schirmer","year":"2006","journal-title":"Toxicology"},{"issue":"1\u20132","key":"10.1016\/j.cbpc.2023.109638_bb0170","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0887-2333(97)00002-7","article-title":"Methodology for demonstrating and measuring the photocytotoxicity of fluoranthene to fish cells in culture","volume":"11","author":"Schirmer","year":"1997","journal-title":"Toxicol Vitr."},{"key":"10.1016\/j.cbpc.2023.109638_bb0175","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.ecoenv.2015.06.012","article-title":"Environmental monitoring of urban streams using a primary fish gill cell culture system (FIGCS)","volume":"120","author":"Schnell","year":"2015","journal-title":"Ecotoxicol. Environ. Saf."},{"issue":"3","key":"10.1016\/j.cbpc.2023.109638_bb0180","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1038\/nprot.2016.029","article-title":"Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia","volume":"11","author":"Schnell","year":"2016","journal-title":"Nat. Protoc."},{"issue":"4","key":"10.1016\/j.cbpc.2023.109638_bb0185","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1002\/etc.4947","article-title":"Applications of the RTgill-W1 cell line for acute whole-effluent toxicity testing: in vitro\u2013in vivo correlation and optimization of exposure conditions","volume":"40","author":"Scott","year":"2021","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2023.109638_bb0190","doi-asserted-by":"crossref","DOI":"10.1002\/etc.5455","article-title":"Fish embryo acute toxicity testing and the RTgill-W1 cell line as in-vitro models for whole effluent toxicity (WET) testing: an in Vitro\/In vivo comparison of chemicals relevant for WET testing","author":"Scott","year":"2022","journal-title":"Environ. Toxicol. Chem."},{"issue":"4","key":"10.1016\/j.cbpc.2023.109638_bb0195","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1177\/026119290403200409","article-title":"Cytotoxicity assays with fish cells as an alternative to the acute lethality test with fish","volume":"32","author":"Segner","year":"2004","journal-title":"ATLA Altern. Lab. Anim."},{"issue":"6","key":"10.1016\/j.cbpc.2023.109638_bb0200","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1007\/s00128-007-9272-x","article-title":"Influence of salinity on PAH uptake from water soluble fraction of crude oil in Tilapia mossambica","volume":"79","author":"Shukla","year":"2007","journal-title":"Bull. Environ. Contam. Toxicol."},{"issue":"2","key":"10.1016\/j.cbpc.2023.109638_bb0205","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1177\/2211068214561025","article-title":"TEER measument techniques for in vitro barrier model systems","volume":"20","author":"Srinivasan","year":"2016","journal-title":"J. Lab. Autom."},{"issue":"2","key":"10.1016\/j.cbpc.2023.109638_bb0210","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1021\/es303505z","article-title":"Predicting fish acute toxicity using a fish gill cell line-based toxicity assay","volume":"47","author":"Tanneberger","year":"2013","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.cbpc.2023.109638_bb0215","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpa.2014.12.005","article-title":"Transepithelial resistance and claudin expression in trout RTgill-W1 cell line: effects of osmoregulatory hormones","author":"Trubitt","year":"2015","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"10.1016\/j.cbpc.2023.109638_bb0220","series-title":"Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms Fifth Edition October 2002","author":"USEPA","year":"2002"},{"issue":"1\u20132","key":"10.1016\/j.cbpc.2023.109638_bb0225","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/S0005-2736(02)00595-3","article-title":"Cultured gill epithelia as models for the freshwater fish gill","volume":"1566","author":"Wood","year":"2002","journal-title":"Biochim. Biophys. Acta Biomembr."},{"issue":"1","key":"10.1016\/j.cbpc.2023.109638_bb0230","doi-asserted-by":"crossref","first-page":"54","DOI":"10.3109\/17435390.2014.889236","article-title":"Toxicity of silver nanoparticles to a fish gill cell line: role of medium composition","volume":"9","author":"Yue","year":"2015","journal-title":"Nanotoxicology"}],"container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045623000935?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045623000935?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T13:23:05Z","timestamp":1736428985000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1532045623000935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8]]},"references-count":46,"alternative-id":["S1532045623000935"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpc.2023.109638","relation":{},"ISSN":["1532-0456"],"issn-type":[{"value":"1532-0456","type":"print"}],"subject":[],"published":{"date-parts":[[2023,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of RTgill-W1 cells epithelial development on transwell inserts: Evaluation of osmotic and toxic challenges","name":"articletitle","label":"Article Title"},{"value":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbpc.2023.109638","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109638"}}