{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T20:21:07Z","timestamp":1787862067300,"version":"build-2784847793"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.cbpc.2026.110636","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T06:41:46Z","timestamp":1786084906000},"page":"110636","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["A cardiovascular dynamic analysis in zebrafish embryos reveals trifloxystrobin-induced cardiovascular dysfunction"],"prefix":"10.1016","volume":"309","author":[{"given":"Jiun-Lin","family":"Horng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueh-Tzu","family":"Kao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Yih","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cbpc.2026.110636_bb0005","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1002\/ps.520","article-title":"The strobilurin fungicides","volume":"58","author":"Bartlett","year":"2002","journal-title":"Pest Manag. Sci."},{"key":"10.1016\/j.cbpc.2026.110636_bb0010","doi-asserted-by":"crossref","first-page":"4193","DOI":"10.1242\/dev.01970","article-title":"Genetic and cellular analyses of zebrafish atrioventricular cushion and valve development","volume":"132","author":"Beis","year":"2005","journal-title":"Development"},{"key":"10.1016\/j.cbpc.2026.110636_bb0015","doi-asserted-by":"crossref","DOI":"10.1117\/1.JBO.26.11.116004","article-title":"Label-free quantitative measurement of cardiovascular dynamics in a zebrafish embryo using frequency-comb-referenced-quantitative phase imaging","volume":"26","author":"Boonruangkan","year":"2021","journal-title":"J. Biomed. Opt."},{"key":"10.1016\/j.cbpc.2026.110636_bb0020","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1111\/bph.15473","article-title":"Zebrafish as a tractable model of human cardiovascular disease","volume":"179","author":"Bowley","year":"2022","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0025","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/j.tcb.2010.06.009","article-title":"The yolk syncytial layer in early zebrafish development","volume":"20","author":"Carvalho","year":"2010","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2023.140747","article-title":"Trifloxystrobin induced developmental toxicity by disturbing the ABC transporters, carbohydrate and lipid metabolism in adult zebrafish","volume":"349","author":"Chen","year":"2024","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2026.110636_bb0035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3001505","article-title":"Cardiac forces regulate zebrafish heart valve delamination by modulating Nfat signaling","volume":"20","author":"Chow","year":"2022","journal-title":"PLoS Biol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0040","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1002\/etc.3520","article-title":"Toxicity of three strobilurins (kresoxim-methyl, pyraclostrobin, and trifloxystrobin) on Daphnia magna","volume":"36","author":"Cui","year":"2017","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2026.110636_bb0045","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/etc.2406","article-title":"Zebrafish as a model system to study toxicology","volume":"33","author":"Dai","year":"2014","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2026.110636_bb0050","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.devcel.2020.10.008","article-title":"Transient nodal signaling in left precursors coordinates opposed asymmetries shaping the heart loop","volume":"55","author":"Desgrange","year":"2020","journal-title":"Dev. Cell"},{"key":"10.1016\/j.cbpc.2026.110636_bb0055","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/bs.mcb.2016.03.041","article-title":"Zebrafish kidney development","volume":"134","author":"Drummond","year":"2016","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0060","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1152\/physrev.00045.2006","article-title":"Regulation of coronary blood flow during exercise","volume":"88","author":"Duncker","year":"2008","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.cbpc.2026.110636_bb0065","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.tcb.2024.10.012","article-title":"Rhythmic forces shaping the zebrafish cardiac system","volume":"35","author":"Fukui","year":"2025","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0070","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1038\/s41598-020-58563-w","article-title":"Automated high-throughput heartbeat quantification in medaka and zebrafish embryos under physiological conditions","volume":"10","author":"Gierten","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cbpc.2026.110636_bb0075","article-title":"Nppa and Nppb act redundantly during zebrafish cardiac development to confine AVC marker expression and reduce cardiac jelly volume","volume":"145","author":"Grassini","year":"2018","journal-title":"Development"},{"key":"10.1016\/j.cbpc.2026.110636_bb0080","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1093\/toxsci\/kfi110","article-title":"Zebrafish as a model vertebrate for investigating chemical toxicity","volume":"86","author":"Hill","year":"2005","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.cbpc.2026.110636_bb0085","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpc.2024.109838","article-title":"Assessing cardiovascular toxicity in zebrafish embryos exposed to copper nanoparticles","volume":"277","author":"Hsiao","year":"2024","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0090","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1038\/nature02028","article-title":"The Wnt\/beta-catenin pathway regulates cardiac valve formation","volume":"425","author":"Hurlstone","year":"2003","journal-title":"Nature"},{"key":"10.1016\/j.cbpc.2026.110636_bb0095","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1021\/es802270t","article-title":"Cardiac arrhythmia is the primary response of embryonic Pacific herring (Clupea pallasi) exposed to crude oil during weathering","volume":"43","author":"Incardona","year":"2009","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0100","doi-asserted-by":"crossref","DOI":"10.3390\/jcdd8040046","article-title":"A heterozygous mutation in cardiac troponin T promotes Ca(2+) dysregulation and adult cardiomyopathy in zebrafish","volume":"8","author":"Kamel","year":"2021","journal-title":"J. Cardiovasc. Dev. Dis."},{"key":"10.1016\/j.cbpc.2026.110636_bb0105","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2023.121537","article-title":"Enantioselective effect of trifloxystrobin in early-stage zebrafish (Danio rerio) embryos: cardiac abnormalities impacted by E,E-trifloxystrobin enantiomer","volume":"327","author":"Kim","year":"2023","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.cbpc.2026.110636_bb0110","doi-asserted-by":"crossref","first-page":"2654","DOI":"10.1002\/etc.2731","article-title":"Dissipation and residues of trifloxystrobin and its metabolite in rice under field conditions","volume":"33","author":"Li","year":"2014","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2026.110636_bb0115","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1016\/j.chemosphere.2018.05.146","article-title":"Developmental toxicity, oxidative stress and immunotoxicity induced by three strobilurins (pyraclostrobin, trifloxystrobin and picoxystrobin) in zebrafish embryos","volume":"207","author":"Li","year":"2018","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2026.110636_bb0120","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1161\/CIRCRESAHA.111.246504","article-title":"Zebrafish in the study of early cardiac development","volume":"110","author":"Liu","year":"2012","journal-title":"Circ. Res."},{"key":"10.1016\/j.cbpc.2026.110636_bb0125","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.ecoenv.2013.10.011","article-title":"Toxic effects of three strobilurins (trifloxystrobin, azoxystrobin and kresoxim-methyl) on mRNA expression and antioxidant enzymes in grass carp (Ctenopharyngodon idella) juveniles","volume":"98","author":"Liu","year":"2013","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.cbpc.2026.110636_bb0130","article-title":"Functional cardiac imaging in zebrafish embryos using standard microscopy and video analysis: applications in environmental and biomedical research","volume":"224","author":"Mitovic","year":"2025","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.cbpc.2026.110636_bb0135","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1002\/etc.2228","article-title":"Acute toxicity of pyraclostrobin and trifloxystrobin to Hyalella azteca","volume":"32","author":"Morrison","year":"2013","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.cbpc.2026.110636_bb0140","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nrm1911","article-title":"VEGF receptor signalling - in control of vascular function","volume":"7","author":"Olsson","year":"2006","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0145","first-page":"1167","article-title":"2016 ESC guidelines for the diagnosis and treatment of acute and chronic heart failure","volume":"69","author":"Ponikowski","year":"2016","journal-title":"Rev. Esp. Cardiol. (Engl. Ed.)"},{"key":"10.1016\/j.cbpc.2026.110636_bb0150","doi-asserted-by":"crossref","first-page":"3607","DOI":"10.1242\/dev.02519","article-title":"Development of heart valves requires Gata4 expression in endothelial-derived cells","volume":"133","author":"Rivera-Feliciano","year":"2006","journal-title":"Development"},{"key":"10.1016\/j.cbpc.2026.110636_bb0155","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1242\/dev.010694","article-title":"High-speed imaging of developing heart valves reveals interplay of morphogenesis and function","volume":"135","author":"Scherz","year":"2008","journal-title":"Development"},{"key":"10.1016\/j.cbpc.2026.110636_bb0160","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/1050-1738(94)90036-1","article-title":"The zebrafish as a model system to study cardiovascular development","volume":"4","author":"Stainier","year":"1994","journal-title":"Trends Cardiovasc. Med."},{"key":"10.1016\/j.cbpc.2026.110636_bb0165","doi-asserted-by":"crossref","first-page":"2846","DOI":"10.1002\/etc.5158","article-title":"Evaluating multiple biochemical markers in Xenopus laevis tadpoles exposed to the pesticides thiacloprid and trifloxystrobin in single and mixed forms","volume":"40","author":"Uckun","year":"2021","journal-title":"Environ. Toxicol. Chem."},{"issue":"11","key":"10.1016\/j.cbpc.2026.110636_bb0170","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1000246","article-title":"Reversing blood flows act through klf2a to ensure normal valvulogenesis in the developing heart","volume":"7","author":"Vermot","year":"2009","journal-title":"PLoS Biol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0175","doi-asserted-by":"crossref","DOI":"10.3390\/molecules29010163","article-title":"Safe application of 75% trifloxystrobin-tebuconazole as water-dispersible granules in Paddy based on residue and dietary risk assessment","volume":"29","author":"Wang","year":"2023","journal-title":"Molecules"},{"key":"10.1016\/j.cbpc.2026.110636_bb0180","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1009690","article-title":"The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics","volume":"17","author":"Wen","year":"2021","journal-title":"PLoS Genet."},{"key":"10.1016\/j.cbpc.2026.110636_bb0185","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1007\/s00244-011-9710-y","article-title":"Environmental fate of fungicides in surface waters of a horticultural-production catchment in southeastern Australia","volume":"62","author":"Wightwick","year":"2012","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0190","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1111\/jfb.12915","article-title":"Genome-wide identification of suitable zebrafish Danio rerio reference genes for normalization of gene expression data by RT-qPCR","volume":"88","author":"Xu","year":"2016","journal-title":"J. Fish Biol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0195","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2020.116277","article-title":"Evaluation and comparison of the mitochondrial and developmental toxicity of three strobilurins in zebrafish embryo\/larvae","volume":"270","author":"Yang","year":"2021","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.cbpc.2026.110636_bb0200","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquatox.2024.106834","article-title":"Combined toxicity of trifloxystrobin and fluopyram to zebrafish embryos and the effect on bone development","volume":"268","author":"Zhang","year":"2024","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.cbpc.2026.110636_bb0205","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.chemosphere.2014.07.100","article-title":"Assessment of trifloxystrobin uptake kinetics, developmental toxicity and mRNA expression in rare minnow embryos","volume":"120","author":"Zhu","year":"2015","journal-title":"Chemosphere"},{"key":"10.1016\/j.cbpc.2026.110636_bb0210","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1002\/tox.21942","article-title":"Effect of trifloxystrobin on hatching, survival, and gene expression of endocrine biomarkers in early life stages of medaka (Oryzias latipes)","volume":"30","author":"Zhu","year":"2015","journal-title":"Environ. Toxicol."}],"container-title":["Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045626001948?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1532045626001948?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T19:44:40Z","timestamp":1787859880000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1532045626001948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":42,"alternative-id":["S1532045626001948"],"URL":"https:\/\/doi.org\/10.1016\/j.cbpc.2026.110636","relation":{},"ISSN":["1532-0456"],"issn-type":[{"value":"1532-0456","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A cardiovascular dynamic analysis in zebrafish embryos reveals trifloxystrobin-induced cardiovascular dysfunction","name":"articletitle","label":"Article Title"},{"value":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cbpc.2026.110636","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110636"}}