{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T11:05:25Z","timestamp":1778497525787,"version":"3.51.4"},"reference-count":82,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinica Chimica Acta"],"published-print":{"date-parts":[[2021,10]]},"DOI":"10.1016\/j.cca.2021.07.013","type":"journal-article","created":{"date-parts":[[2021,7,14]],"date-time":"2021-07-14T11:20:15Z","timestamp":1626261615000},"page":"229-243","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Microfluidic devices for glycobiomarker detection in cancer"],"prefix":"10.1016","volume":"521","author":[{"given":"M. Lu\u00edsa S.","family":"Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.cca.2021.07.013_b0005","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/nrc3982","article-title":"Glycosylation in cancer: mechanisms and clinical implications","volume":"15","author":"Pinho","year":"2015","journal-title":"Nat. Rev. Cancer"},{"issue":"3-4","key":"10.1016\/j.cca.2021.07.013_b0010","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s12014-008-9017-9","article-title":"The potentials of glycomics in biomarker discovery","volume":"4","author":"Kam","year":"2008","journal-title":"Clin. Proteom."},{"key":"10.1016\/j.cca.2021.07.013_b0015","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.cellimm.2018.03.007","article-title":"Glycosylation in cancer: selected roles in tumour progression, immune modulation and metastasis","volume":"333","author":"Rodrigues","year":"2018","journal-title":"Cell. Immunol."},{"key":"10.1016\/j.cca.2021.07.013_b0020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1559-0275-10-13","article-title":"Translation of proteomic biomarkers into FDA approved cancer diagnostics: issues and challenges","volume":"10","author":"F\u00fcz\u00e9ry","year":"2013","journal-title":"Clin. Proteomics"},{"key":"10.1016\/j.cca.2021.07.013_b0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/490531","article-title":"Glycosylation-based serum biomarkers for cancer diagnostics and prognostics","volume":"2015","author":"Kirwan","year":"2015","journal-title":"Biomed. Res. Int."},{"issue":"25","key":"10.1016\/j.cca.2021.07.013_b0030","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1056\/NEJM199306243282502","article-title":"Early recognition of hepatocellular carcinoma based on altered profiles of alpha-fetoprotein","volume":"328","author":"Sato","year":"1993","journal-title":"N. Eng. J. Med."},{"key":"10.1016\/j.cca.2021.07.013_b0035","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1093\/clinchem\/48.7.1021","article-title":"Quantification and utility of monosialylated \u03b1-fetoprotein in the diagnosis of hepatocellular carcinoma with nondiagnostic serum total \u03b1-fetoprotein","volume":"48","author":"Poon","year":"2002","journal-title":"Clin. Chem."},{"key":"10.1016\/j.cca.2021.07.013_b0040","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1046\/j.1440-1746.1999.01882.x","article-title":"Highly enhanced fucosylation of serum glycoproteins in patients with hepatocellular carcinoma","volume":"14","author":"Naitoh","year":"1999","journal-title":"J. Gastroenterol. Hepatol."},{"key":"10.1016\/j.cca.2021.07.013_b0045","doi-asserted-by":"crossref","first-page":"2496","DOI":"10.1039\/c0an00204f","article-title":"Measurement of biomarker proteins for point-of-care early detection and monitoring of cancer","volume":"135","author":"Rusling","year":"2010","journal-title":"Analyst"},{"key":"10.1016\/j.cca.2021.07.013_b0050","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.mne.2019.01.003","article-title":"30 years of microfluidics","volume":"2","author":"Convery","year":"2019","journal-title":"Micro Nano Eng."},{"issue":"3","key":"10.1016\/j.cca.2021.07.013_b0055","doi-asserted-by":"crossref","first-page":"131","DOI":"10.2144\/000113816","article-title":"Microfluidics, macro-impacts","volume":"52","author":"Perkel","year":"2012","journal-title":"Biotechniques"},{"key":"10.1016\/j.cca.2021.07.013_b0060","doi-asserted-by":"crossref","first-page":"602659","DOI":"10.3389\/fbioe.2020.602659","article-title":"Microfluidic point-of-care testing: commercial landscape and future directions","volume":"8","author":"Sachdeva","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.cca.2021.07.013_b0065","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.aca.2011.11.044","article-title":"Green chemistry and the evolution of flow analysis","volume":"714","author":"Melchert","year":"2012","journal-title":"Anal. Chim. Acta"},{"issue":"1-3","key":"10.1016\/j.cca.2021.07.013_b0070","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0003-2670(99)00611-X","article-title":"Development of an electrochemical flow injection immunoassay (FIIA) for the real-time monitoring of biospecific interactions","volume":"400","author":"Killard","year":"1999","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.cca.2021.07.013_b0075","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.elecom.2014.05.013","article-title":"Miniaturization of electrochemical flow devices","volume":"45","author":"Campo","year":"2014","journal-title":"Electrochem. Commun."},{"key":"10.1016\/j.cca.2021.07.013_b0080","doi-asserted-by":"crossref","first-page":"1752","DOI":"10.1039\/b712394a","article-title":"Screen-printed microfluidic device for electrochemical immunoassay","volume":"7","author":"Dong","year":"2007","journal-title":"Lab Chip"},{"issue":"11","key":"10.1016\/j.cca.2021.07.013_b0085","doi-asserted-by":"crossref","first-page":"115007","DOI":"10.1088\/0960-1317\/19\/11\/115007","article-title":"Screen printing as a holistic manufacturing method for multifunctional microsystems and microreactors","volume":"19","author":"Bejarano","year":"2009","journal-title":"J. Micromech. Microeng."},{"issue":"1-6","key":"10.1016\/j.cca.2021.07.013_b0090","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/0925-4005(90)80209-I","article-title":"Miniaturized total chemical analysis systems: a novel concept for chemical sensing","volume":"1","author":"Manz","year":"1990","journal-title":"Sens. Actuators B Chem."},{"key":"10.1016\/j.cca.2021.07.013_b0095","doi-asserted-by":"crossref","first-page":"3263","DOI":"10.3390\/ijms12053263","article-title":"Microfluidic mixing: a review","volume":"12","author":"Lee","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.cca.2021.07.013_b0100","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3390\/mi8010028","article-title":"Microfluidic techniques for analytes concentration","volume":"8","author":"Zhao","year":"2017","journal-title":"Micromachines"},{"issue":"20","key":"10.1016\/j.cca.2021.07.013_b0105","doi-asserted-by":"crossref","first-page":"2677","DOI":"10.4155\/bio.15.164","article-title":"Microfluidic devices in diagnostics: what does the future hold?","volume":"7","author":"Lazar","year":"2015","journal-title":"Bioanalysis"},{"issue":"2","key":"10.1016\/j.cca.2021.07.013_b0110","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.chempr.2017.01.009","article-title":"Small but perfectly formed? Successes, challenges, and opportunities for microfluidics in the chemical and biological sciences","volume":"2","author":"Chiu","year":"2017","journal-title":"Chem."},{"issue":"2","key":"10.1016\/j.cca.2021.07.013_b0115","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s10544-005-1587-y","article-title":"Modeling and optimization of high-sensitivity, low-volume microfluidic-based surface immunoassays","volume":"7","author":"Zimmermann","year":"2005","journal-title":"Biomed. Microdevices"},{"issue":"1","key":"10.1016\/j.cca.2021.07.013_b0120","doi-asserted-by":"crossref","first-page":"014115","DOI":"10.1063\/1.4977198","article-title":"Evaluation of disposable microfluidic chip design for automated and fast Immunoassays","volume":"11","author":"Wang","year":"2017","journal-title":"Biomicrofluidics"},{"key":"10.1016\/j.cca.2021.07.013_b0125","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.sbsr.2015.11.003","article-title":"Lab-on-chip system combining a microfluidic-ELISA with an array of amorphous silicon photosensors for the detection of celiac disease epitopes","volume":"6","author":"Costantini","year":"2015","journal-title":"Sens. Biosensing Res."},{"issue":"5","key":"10.1016\/j.cca.2021.07.013_b0130","doi-asserted-by":"crossref","first-page":"e1457","DOI":"10.1002\/wnan.1457","article-title":"ELISA-type assays of trace biomarkers using microfluidic methods","volume":"9","author":"Dong","year":"2017","journal-title":"Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol."},{"issue":"4","key":"10.1016\/j.cca.2021.07.013_b0135","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1039\/C7AN01671A","article-title":"Microfluidic ELISA employing an enzyme substrate and product species with similar detection properties","volume":"143","author":"Giri","year":"2018","journal-title":"Analyst"},{"issue":"22","key":"10.1016\/j.cca.2021.07.013_b0140","doi-asserted-by":"crossref","first-page":"6625","DOI":"10.1039\/C9AN01491H","article-title":"Enzyme-linked immunosorbent assay utilizing thin-layered microfluidics","volume":"144","author":"Nakao","year":"2019","journal-title":"Analyst"},{"issue":"19","key":"10.1016\/j.cca.2021.07.013_b0145","doi-asserted-by":"crossref","first-page":"8472","DOI":"10.1039\/C5AY00264H","article-title":"Development of an automated on-chip bead-based ELISA platform","volume":"7","author":"Campbell","year":"2015","journal-title":"Anal. Methods"},{"key":"10.1016\/j.cca.2021.07.013_b0150","doi-asserted-by":"crossref","first-page":"24598","DOI":"10.1038\/srep24598","article-title":"Automated microfluidic platform of bead-based electrochemical immunosensor integrated with bioreactor for continual monitoring of cell secreted biomarkers","volume":"6","author":"Riahi","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cca.2021.07.013_b0155","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1042\/EBC20150013","article-title":"Lab-on-chip systems for integrated bioanalyses","volume":"60","author":"Conde","year":"2016","journal-title":"Essays Biochem."},{"issue":"6","key":"10.1016\/j.cca.2021.07.013_b0160","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1021\/pr0600024","article-title":"Different immunoaffinity fractionation strategies to characterize the human plasma proteome","volume":"5","author":"Gong","year":"2006","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.cca.2021.07.013_b0165","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1002\/elps.201600413","article-title":"Liquid-phase based separation systems for depletion, prefractionation, and enrichment of proteins in biological fluids and matrices for in-depth proteomics analysis - an update covering the period 2014\u20132016","volume":"38","author":"El Rassi","year":"2017","journal-title":"Electrophoresis"},{"key":"10.1016\/j.cca.2021.07.013_b0170","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jprot.2017.08.009","article-title":"Protein biomarkers for early detection of diseases: the decisive contribution of combinatorial peptide ligand libraries","volume":"188","author":"Boschetti","year":"2018","journal-title":"J. Proteomics"},{"issue":"11","key":"10.1016\/j.cca.2021.07.013_b0175","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1074\/mcp.R200007-MCP200","article-title":"The human plasma proteome: history, character, and diagnostic prospects","volume":"1","author":"Anderson","year":"2002","journal-title":"Mol. Cell. Proteomics"},{"issue":"5","key":"10.1016\/j.cca.2021.07.013_b0180","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1007\/s00542-009-0783-8","article-title":"Microfluidic devices for sample pretreatment and applications","volume":"15","author":"Chen","year":"2009","journal-title":"Microsyst. Technol."},{"key":"10.1016\/j.cca.2021.07.013_b0185","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2017.07.043","article-title":"Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: a critical review","volume":"986","author":"Sonker","year":"2017","journal-title":"Anal. Chim. Acta"},{"issue":"6","key":"10.1016\/j.cca.2021.07.013_b0190","doi-asserted-by":"crossref","first-page":"063001","DOI":"10.1088\/1361-6439\/aab225","article-title":"Applications of antibodies in microfluidics-based analytical systems: challenges and strategies for success","volume":"28","author":"O\u2019Kennedy","year":"2018","journal-title":"J. Micromech. Microeng."},{"issue":"9","key":"10.1016\/j.cca.2021.07.013_b0195","doi-asserted-by":"crossref","first-page":"3496","DOI":"10.1021\/am200716x","article-title":"Minimizing nonspecific protein adsorption in liquid crystal immunoassays by using surfactants","volume":"3","author":"Zhang","year":"2011","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.cca.2021.07.013_b0200","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3390\/bios9010015","article-title":"Suppressing non-specific binding of proteins onto electrode surfaces in the development of electrochemical immunosensors","volume":"9","author":"Contreras-Naranjo","year":"2019","journal-title":"Biosensors"},{"issue":"9","key":"10.1016\/j.cca.2021.07.013_b0205","doi-asserted-by":"crossref","first-page":"3938","DOI":"10.1021\/ac300771z","article-title":"Comparison of biocompatibility and adsorption properties of different plastics for advanced microfluidic cell and tissue culture models","volume":"84","author":"van Midwoud","year":"2012","journal-title":"Anal. Chem."},{"key":"10.1016\/j.cca.2021.07.013_b0210","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1002\/pmic.200900458","article-title":"Label-free detection techniques for protein microarrays: prospects, merits and challenges","volume":"10","author":"Ray","year":"2010","journal-title":"Proteomics"},{"issue":"3","key":"10.1016\/j.cca.2021.07.013_b0215","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s12154-013-0094-5","article-title":"Fluorescent labeling and modification of proteins","volume":"6","author":"Toseland","year":"2013","journal-title":"J. Chem. Biol."},{"key":"10.1016\/j.cca.2021.07.013_b0220","doi-asserted-by":"crossref","first-page":"228","DOI":"10.3390\/microarrays4020228","article-title":"Label and label-free detection techniques for protein microarrays","volume":"4","author":"Syahir","year":"2015","journal-title":"Microarrays"},{"key":"10.1016\/j.cca.2021.07.013_b0225","doi-asserted-by":"crossref","first-page":"5944","DOI":"10.1039\/c3cs60077g","article-title":"Electrical biosensors and the label free detection of protein disease biomarkers","volume":"42","author":"Luo","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.cca.2021.07.013_b0230","doi-asserted-by":"crossref","unstructured":"M. Varma, Performance limitations of label-free sensors in molecular diagnosis with complex samples, in: B.L. Miller, B.T. Cunningham (Eds.), Frontiers in Biological Detection: From Nanosensors to Systems VIII, Proc. SPIE 9725, 2016, pp. 97250P1-4.","DOI":"10.1117\/12.2211655"},{"issue":"6","key":"10.1016\/j.cca.2021.07.013_b0235","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1039\/C6AN02445A","article-title":"A critical insight into the development pipeline of microfluidic immunoassay devices for the sensitive quantitation of protein biomarkers at the point of care","volume":"142","author":"Barbosa","year":"2017","journal-title":"Analysts"},{"issue":"11","key":"10.1016\/j.cca.2021.07.013_b0240","doi-asserted-by":"crossref","first-page":"2952","DOI":"10.1021\/acssensors.9b01430","article-title":"Microfluidic immunoassay system for rapid detection and semi-quantitative determination of a potential serum biomarker mesothelin","volume":"4","author":"Duan","year":"2019","journal-title":"ACS Sens."},{"issue":"8","key":"10.1016\/j.cca.2021.07.013_b0245","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1016\/j.bios.2010.01.019","article-title":"A dual gold nanoparticle conjugate-based lateral flow assay (LFA) method for the analysis of troponin I","volume":"25","author":"Choi","year":"2010","journal-title":"Biosens. Bioelectron."},{"issue":"1","key":"10.1016\/j.cca.2021.07.013_b0250","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.bios.2011.09.002","article-title":"Quantum dot-based immunochromatography test strip for rapid, quantitative and sensitive detection of alpha fetoprotein","volume":"30","author":"Yang","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.cca.2021.07.013_b0255","doi-asserted-by":"crossref","first-page":"577","DOI":"10.3390\/bios5030577","article-title":"Point-of-care diagnosis in low resource settings: present status and future role of microfluidics","volume":"5","author":"Sharma","year":"2015","journal-title":"Biosensors"},{"key":"10.1016\/j.cca.2021.07.013_b0260","doi-asserted-by":"crossref","first-page":"20297","DOI":"10.1038\/srep20297","article-title":"Integrated microfluidic lectin barcode platform for high-performance focused glycomic profiling","volume":"6","author":"Shang","year":"2016","journal-title":"Sci. Rep."},{"issue":"3","key":"10.1016\/j.cca.2021.07.013_b0265","doi-asserted-by":"crossref","first-page":"034107","DOI":"10.1063\/1.4882778","article-title":"On-chip lectin microarray for glycoprofiling of different gastritis types and gastric cancer","volume":"8","author":"Roy","year":"2014","journal-title":"Biomicrofluidics"},{"key":"10.1016\/j.cca.2021.07.013_b0270","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.bios.2015.12.058","article-title":"DNA aptamer-based sandwich microfluidic assays for dual quantification and multi-glycan profiling of cancer biomarkers","volume":"79","author":"Jolly","year":"2016","journal-title":"Biosens. Biolectron."},{"key":"10.1016\/j.cca.2021.07.013_b0275","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1021\/ac503735e","volume":"87","author":"Kaya","year":"2015","journal-title":"Anal. Chem."},{"key":"10.1016\/j.cca.2021.07.013_b0280","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.nbt.2018.09.004","article-title":"Exploring sialyl-Tn expression in microfluidic-isolated circulating tumour cells: a novel biomarker and an analytical tool for precision oncology applications","volume":"49","author":"Neves","year":"2019","journal-title":"N. Biotechnol."},{"key":"10.1016\/j.cca.2021.07.013_b0285","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.3389\/fonc.2020.01774","article-title":"Phenotypic analysis of urothelial exfoliated cells in bladder cancer via microfluidic immunoassays: sialyl-Tn as a novel biomarker in liquid biopsies","volume":"10","author":"Carvalho","year":"2020","journal-title":"Front. Oncol."},{"issue":"10","key":"10.1016\/j.cca.2021.07.013_b0290","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1039\/C4LC00041B","article-title":"Microfluidic immunocapture of circulating pancreatic cells using parallel EpCAM and MUC1 capture: characterization, optimization and downstream analysis","volume":"14","author":"Thege","year":"2014","journal-title":"Lab Chip"},{"issue":"9","key":"10.1016\/j.cca.2021.07.013_b0295","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1080\/09205063.2020.1748332","article-title":"A microfluidic system based on the monoclonal antibody BCMab1 specifically captures circulating tumor cells from bladder cancer patients","volume":"31","author":"Wang","year":"2020","journal-title":"J. Biomater. Sci. Polym. Ed."},{"issue":"10","key":"10.1016\/j.cca.2021.07.013_b0300","doi-asserted-by":"crossref","first-page":"4490","DOI":"10.1021\/pr400549e","article-title":"Comparative profiling of N-glycans isolated from serum samples of ovarian cancer patients and analyzed by microchip electrophoresis","volume":"12","author":"Mitra","year":"2013","journal-title":"J. Proteome Res."},{"issue":"8","key":"10.1016\/j.cca.2021.07.013_b0305","doi-asserted-by":"crossref","first-page":"3621","DOI":"10.1021\/ac203431s","article-title":"N-glycan profiling by microchip electrophoresis to differentiate disease-states related to esophageal adenocarcinoma","volume":"84","author":"Mitra","year":"2012","journal-title":"Anal. Chem."},{"key":"10.1016\/j.cca.2021.07.013_b0310","doi-asserted-by":"crossref","first-page":"3056","DOI":"10.1002\/pmic.201500554","article-title":"Development of on-chip fully automated immunoassay system \u201c\u03bcTASWako i30\u201d to measure the changes in glycosylation profiles of alpha-fetoprotein in patients with hepatocellular carcinoma","volume":"16","author":"Kurosawa","year":"2016","journal-title":"Proteomics"},{"key":"10.1016\/j.cca.2021.07.013_b0315","doi-asserted-by":"crossref","first-page":"470","DOI":"10.3390\/ijms18020470","article-title":"An automated micro-total immunoassay system for measuring cancer-associated 2,3-linked sialyl N-glycan-carrying prostate-specific antigen may improve the accuracy of prostate cancer diagnosis","volume":"18","author":"Ishikawa","year":"2017","journal-title":"Int. J. Mol. Sci."},{"issue":"23","key":"10.1016\/j.cca.2021.07.013_b0320","doi-asserted-by":"crossref","first-page":"6444","DOI":"10.1039\/C6AN01746K","article-title":"Lectin-based lateral flow assay: proof-of-concept","volume":"141","author":"Damborsk\u00fd","year":"2016","journal-title":"Analyst"},{"key":"10.1016\/j.cca.2021.07.013_b0325","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1039\/C6AN02445A","article-title":"A critical insight into the development pipeline of microfluidic immunoassay devices for sensitive quantitation of protein biomarkers at point-of-care","volume":"142","author":"Barbosa","year":"2017","journal-title":"Analyst"},{"key":"10.1016\/j.cca.2021.07.013_b0330","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.copbio.2019.11.022","article-title":"Microfluidic systems for cancer diagnostics","volume":"65","author":"Garcia-Cordero","year":"2020","journal-title":"Curr. Opin. Biotechnol."},{"issue":"2","key":"10.1016\/j.cca.2021.07.013_b0335","first-page":"165","article-title":"Cancer serum biomarkers based on aberrant post-translational modifications of glycoproteins: clinical value and discovery strategies","volume":"1856","author":"Silva","year":"2015","journal-title":"Biochim. Biophys. Acta, Proteins Proteomics"},{"issue":"21","key":"10.1016\/j.cca.2021.07.013_b0340","doi-asserted-by":"crossref","first-page":"6073","DOI":"10.1039\/C6AN01330A","article-title":"Designed miniaturization of microfluidic biosensor platforms using the stop-flow technique","volume":"141","author":"Dincer","year":"2016","journal-title":"Analyst"},{"key":"10.1016\/j.cca.2021.07.013_b0345","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1038\/s41467-018-04813-5","article-title":"Advances in highly doped upconversion nanoparticles","volume":"9","author":"Wen","year":"2018","journal-title":"Nat. Commun."},{"issue":"9","key":"10.1016\/j.cca.2021.07.013_b0350","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1039\/C9AN00081J","article-title":"Recent advances in nanomaterial-based electrochemical and optical sensing platforms for microRNA assays","volume":"144","author":"Wang","year":"2019","journal-title":"Analyst"},{"key":"10.1016\/j.cca.2021.07.013_b0355","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1007\/s10404-017-1907-6","article-title":"Capillary flow-driven blood plasma separation and on-chip analyte detection in microfluidic devices","volume":"21","author":"Maria","year":"2017","journal-title":"Microfluid. Nanofluidics"},{"key":"10.1016\/j.cca.2021.07.013_b0360","doi-asserted-by":"crossref","first-page":"15345","DOI":"10.1038\/s41598-018-33738-8","article-title":"A microfluidic device for simultaneous extraction of plasma, red blood cells, and on-chip white blood cell trapping","volume":"8","author":"Kuan","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cca.2021.07.013_b0365","doi-asserted-by":"crossref","first-page":"336","DOI":"10.3390\/mi12030336","article-title":"A novel microfluidic device for blood plasma filtration","volume":"12","author":"Al-aqbi","year":"2021","journal-title":"Micromachines"},{"key":"10.1016\/j.cca.2021.07.013_b0370","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1007\/s10544-008-9190-7","article-title":"Sample preparation: the weak link in microfluidics-based biodetection","volume":"10","author":"Mariella","year":"2008","journal-title":"Biomed. Microdevices"},{"issue":"2","key":"10.1016\/j.cca.2021.07.013_b0375","doi-asserted-by":"crossref","first-page":"024126","DOI":"10.1063\/1.4946870","article-title":"Restraining non-specific adsorption of protein using Parylene C-caulked polydimethylsiloxane","volume":"10","author":"Liu","year":"2016","journal-title":"Biomicrofluidics"},{"key":"10.1016\/j.cca.2021.07.013_b0380","doi-asserted-by":"crossref","first-page":"2488","DOI":"10.3390\/s19112488","article-title":"Non-specific adsorption reduction methods in biosensing","volume":"19","author":"Lichtenberg","year":"2019","journal-title":"Sensors"},{"issue":"12","key":"10.1016\/j.cca.2021.07.013_b0385","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1038\/s41565-019-0566-z","article-title":"An antifouling coating that enables affinity-based electrochemical biosensing in complex biological fluids","volume":"14","author":"Sabat\u00e9 del R\u00edo","year":"2019","journal-title":"Nat. Nanotechnol."},{"issue":"38","key":"10.1016\/j.cca.2021.07.013_b0390","doi-asserted-by":"crossref","first-page":"10202","DOI":"10.1073\/pnas.1704961114","article-title":"Combined circulating tumor DNA and protein biomarker-based liquid biopsy for the earlier detection of pancreatic cancers","volume":"114","author":"Cohen","year":"2017","journal-title":"PNAS"},{"key":"10.1016\/j.cca.2021.07.013_b0395","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s12967-018-1432-8","article-title":"Improving cancer detection through combinations of cancer and immune biomarkers: a modelling approach","volume":"16","author":"Eftimie","year":"2018","journal-title":"J. Transl. Med."},{"issue":"14","key":"10.1016\/j.cca.2021.07.013_b0400","doi-asserted-by":"crossref","first-page":"8124","DOI":"10.1039\/D0RA10200H","article-title":"A high-performance microfluidic detection platform to conduct a novel multiple-biomarker panel for ovarian cancer screening","volume":"11","author":"Wu","year":"2021","journal-title":"RSC Adv."},{"issue":"8","key":"10.1016\/j.cca.2021.07.013_b0405","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.tibtech.2017.03.013","article-title":"Multiplexed point-of-care testing \u2013 xPOCT","volume":"35","author":"Dincer","year":"2017","journal-title":"Trends Biotechnol."},{"issue":"15","key":"10.1016\/j.cca.2021.07.013_b0410","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.1039\/C9LC00400A","article-title":"Clinical translation of microfluidic sensor devices: focus on calibration and analytical robustness","volume":"19","author":"Gowers","year":"2019","journal-title":"Lab Chip"}],"container-title":["Clinica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898121002539?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898121002539?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T15:01:10Z","timestamp":1757862070000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009898121002539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10]]},"references-count":82,"alternative-id":["S0009898121002539"],"URL":"https:\/\/doi.org\/10.1016\/j.cca.2021.07.013","relation":{},"ISSN":["0009-8981"],"issn-type":[{"value":"0009-8981","type":"print"}],"subject":[],"published":{"date-parts":[[2021,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Microfluidic devices for glycobiomarker detection in cancer","name":"articletitle","label":"Article Title"},{"value":"Clinica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cca.2021.07.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}