{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:56:31Z","timestamp":1786985791774,"version":"build-2736575974"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinica Chimica Acta"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1016\/j.cca.2022.05.025","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T02:06:03Z","timestamp":1654653963000},"page":"130-136","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["T-cell proliferation assay for the detection of SARS-CoV-2-specific T-cells"],"prefix":"10.1016","volume":"532","author":[{"given":"Chang","family":"Chu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Sch\u00f6nbrunn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saban","family":"Elitok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Kern","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"Schnatbaum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Wenschuh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Klemm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"von Baehr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard K.","family":"Kr\u00e4mer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berthold","family":"Hocher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.cca.2022.05.025_b0005","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-92521-4","article-title":"Immunodominant T-cell epitopes from the SARS-CoV-2 spike antigen reveal robust pre-existing T-cell immunity in unexposed individuals","volume":"11","author":"Mahajan","year":"2021","journal-title":"Sci. Rep."},{"issue":"7","key":"10.1016\/j.cca.2022.05.025_b0010","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1016\/j.cell.2021.02.026","article-title":"Human neutralizing antibodies against SARS-CoV-2 require intact Fc effector functions for optimal therapeutic protection","volume":"184","author":"Winkler","year":"2021","journal-title":"Cell"},{"issue":"5","key":"10.1016\/j.cca.2022.05.025_b0015","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1038\/s41590-021-00902-8","article-title":"Robust SARS-CoV-2-specific T cell immunity is maintained at 6 months following primary infection","volume":"22","author":"Zuo","year":"2021","journal-title":"Nat. Immunol."},{"issue":"7","key":"10.1016\/j.cca.2022.05.025_b0020","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1016\/j.cmi.2021.03.010","article-title":"SARS-CoV-2 specific T-cell immunity in COVID-19 convalescent patients and unexposed controls measured by ex vivo ELISpot assay","volume":"27","author":"Cassaniti","year":"2021","journal-title":"Clin. Microbiol. Infect."},{"issue":"6","key":"10.1016\/j.cca.2022.05.025_b0025","doi-asserted-by":"crossref","first-page":"401","DOI":"10.14348\/molcells.2021.0079","article-title":"Phenotypes and Functions of SARS-CoV-2-Reactive T Cells","volume":"44","author":"Jung","year":"2021","journal-title":"Mol. Cells"},{"key":"10.1016\/j.cca.2022.05.025_b0030","article-title":"Pre-existing polymerase-specific T cells expand in abortive seronegative SARS-CoV-2","author":"Swadling","year":"2021","journal-title":"Nature"},{"issue":"6564","key":"10.1016\/j.cca.2022.05.025_b0035","doi-asserted-by":"crossref","DOI":"10.1126\/science.abh1823","article-title":"Cross-reactive CD4(+) T cells enhance SARS-CoV-2 immune responses upon infection and vaccination","volume":"374","author":"Loyal","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.cca.2022.05.025_b0040","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.ijid.2021.09.073","article-title":"Performance of the T-SPOT.COVID test for detecting SARS-CoV-2-responsive T cells","volume":"113","author":"Kruse","year":"2021","journal-title":"Int. J .Infect. Dis."},{"issue":"4","key":"10.1016\/j.cca.2022.05.025_b0045","doi-asserted-by":"crossref","first-page":"2765","DOI":"10.1007\/s40121-021-00521-8","article-title":"Preliminary Evaluation of QuantiFERON SARS-CoV-2 and QIAreach Anti-SARS-CoV-2 Total Test in Recently Vaccinated Individuals","volume":"10","author":"Jaganathan","year":"2021","journal-title":"Infect. Dis. Ther."},{"issue":"5","key":"10.1016\/j.cca.2022.05.025_b0050","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1038\/s41587-021-00920-9","article-title":"COVID-19 testing turns to T cells","volume":"39","author":"Sheridan","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cca.2022.05.025_b0055","doi-asserted-by":"crossref","unstructured":"S.C. Dalai, J.N. Dines, T.M. Snyder, R.M. Gittelman, T. Eerkes, P. Vaney, et al., Clinical Validation of a Novel T-cell Receptor Sequencing Assay for Identification of Recent or Prior SARS-CoV-2 Infection, (2021) 2021.01.06.21249345.","DOI":"10.1101\/2021.01.06.21249345"},{"issue":"3","key":"10.1016\/j.cca.2022.05.025_b0060","doi-asserted-by":"crossref","first-page":"580","DOI":"10.4049\/jimmunol.2000811","article-title":"A Highly Specific Assay for the Detection of SARS-CoV-2-Reactive CD4(+) and CD8(+) T Cells in COVID-19 Patients","volume":"206","author":"Zelba","year":"2021","journal-title":"J. Immunol."},{"issue":"1","key":"10.1016\/j.cca.2022.05.025_b0065","doi-asserted-by":"crossref","first-page":"2100285","DOI":"10.1183\/13993003.00285-2021","article-title":"Rapid, simplified whole blood-based multiparameter assay to quantify and phenotype SARS-CoV-2-specific T-cells","volume":"59","author":"Riou","year":"2022","journal-title":"Eur. Respir. J."},{"issue":"17","key":"10.1016\/j.cca.2022.05.025_b0070","doi-asserted-by":"crossref","DOI":"10.1172\/JCI152379","article-title":"Rapid measurement of SARS-CoV-2 spike T cells in whole blood from vaccinated and naturally infected individuals","volume":"131","author":"Tan","year":"2021","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.cca.2022.05.025_b0075","article-title":"Commercialized kits to assess T-cell responses against SARS-CoV-2 S peptides","author":"Mart\u00ednez-Gallo","year":"2021","journal-title":"Med. Cl\u00edn."},{"issue":"2","key":"10.1016\/j.cca.2022.05.025_b0080","doi-asserted-by":"crossref","first-page":"286.e7","DOI":"10.1016\/j.cmi.2020.09.051","article-title":"A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients","volume":"27","author":"Petrone","year":"2021","journal-title":"Clin. Microbiol. Infect."},{"issue":"9","key":"10.1016\/j.cca.2022.05.025_b0085","doi-asserted-by":"crossref","first-page":"e3130","DOI":"10.1093\/cid\/ciaa1537","article-title":"Interferon-gamma Release Assay for Accurate Detection of Severe Acute Respiratory Syndrome Coronavirus 2 T-Cell Response","volume":"73","author":"Murugesan","year":"2021","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.cca.2022.05.025_b0090","doi-asserted-by":"crossref","first-page":"688436","DOI":"10.3389\/fimmu.2021.688436","article-title":"Broad T Cell Targeting of Structural Proteins After SARS-CoV-2 Infection: High Throughput Assessment of T Cell Reactivity Using an Automated Interferon Gamma Release Assay","volume":"12","author":"Brand","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.cca.2022.05.025_b0095","doi-asserted-by":"crossref","unstructured":"D. Huzly, M. Panning, F. Smely, M. Enders, J. Komp, D. Steinmann, Validation and performance evaluation of a novel interferon-\u03b3 release assay for the detection of SARS-CoV-2 specific T-cell response, (2021) 2021.07.17.21260316.","DOI":"10.1101\/2021.07.17.21260316"},{"key":"10.1016\/j.cca.2022.05.025_b0100","doi-asserted-by":"crossref","first-page":"113036","DOI":"10.1016\/j.jim.2021.113036","article-title":"Lymphocyte transformation test: History and current approaches","volume":"493","author":"Sachs","year":"2021","journal-title":"J. Immunol. Methods"},{"issue":"7","key":"10.1016\/j.cca.2022.05.025_b0105","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1097\/HJH.0000000000002843","article-title":"2021 European Society of Hypertension practice guidelines for office and out-of-office blood pressure measurement","volume":"39","author":"Stergiou","year":"2021","journal-title":"J. Hypertens."},{"issue":"7833","key":"10.1016\/j.cca.2022.05.025_b0110","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41586-020-2598-9","article-title":"SARS-CoV-2-reactive T cells in healthy donors and patients with COVID-19","volume":"587","author":"Braun","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.cca.2022.05.025_b0115","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.3762\/bjoc.10.118","article-title":"Automated solid-phase peptide synthesis to obtain therapeutic peptides","volume":"10","author":"Made","year":"2014","journal-title":"Beilstein. J. Org. Chem."},{"issue":"7","key":"10.1016\/j.cca.2022.05.025_b0120","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.3201\/eid2607.200841","article-title":"Severe Acute Respiratory Syndrome Coronavirus 2-Specific Antibody Responses in Coronavirus Disease Patients","volume":"26","author":"Okba","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.cca.2022.05.025_b0125","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/978-981-32-9717-3_4","article-title":"Signal Transduction Via Co-stimulatory and Co-inhibitory Receptors","volume":"1189","author":"Ogawa","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"issue":"11","key":"10.1016\/j.cca.2022.05.025_b0130","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1093\/ckj\/sfab152","article-title":"Humoral response to a third injection of BNT162b2 vaccine in patients on maintenance haemodialysis","volume":"14","author":"Dekervel","year":"2021","journal-title":"Clin. Kidney J."},{"key":"10.1016\/j.cca.2022.05.025_b0135","doi-asserted-by":"crossref","unstructured":"L. Timmermann, B. Globke, G. Lurje, M. Schmelzle, W. Sch\u00f6ning, R. \u00d6llinger, et al., Humoral Immune Response following SARS-CoV-2 Vaccination in Liver Transplant Recipients, 9(12) (2021) 1422.","DOI":"10.3390\/vaccines9121422"},{"issue":"5","key":"10.1016\/j.cca.2022.05.025_b0140","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1007\/s11357-021-00471-6","article-title":"Comparison of antibody and T cell responses elicited by BBIBP-CorV (Sinopharm) and BNT162b2 (Pfizer-BioNTech) vaccines against SARS-CoV-2 in healthy adult humans","volume":"43","author":"V\u00e1lyi-Nagy","year":"2021","journal-title":"Geroscience"},{"issue":"24","key":"10.1016\/j.cca.2022.05.025_b0145","doi-asserted-by":"crossref","first-page":"e85","DOI":"10.1056\/NEJMoa2114228","article-title":"Waning Immunity after the BNT162b2 Vaccine in Israel","volume":"385","author":"Goldberg","year":"2021","journal-title":"N. Engl. J. Med."},{"issue":"19","key":"10.1016\/j.cca.2022.05.025_b0150","doi-asserted-by":"crossref","first-page":"11034","DOI":"10.1128\/JVI.01505-14","article-title":"Virus-specific memory CD8 T cells provide substantial protection from lethal severe acute respiratory syndrome coronavirus infection","volume":"88","author":"Channappanavar","year":"2014","journal-title":"J. Virol."},{"issue":"9","key":"10.1016\/j.cca.2022.05.025_b0155","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.it.2005.07.005","article-title":"Measuring Ag-specific immune responses: understanding immunopathogenesis and improving diagnostics in infectious disease, autoimmunity and cancer","volume":"26","author":"Kern","year":"2005","journal-title":"Trends Immunol."},{"key":"10.1016\/j.cca.2022.05.025_b0160","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.3389\/fimmu.2017.01809","article-title":"Life and Death of Activated T Cells: How Are They Different from Naive T Cells?","volume":"8","author":"Zhan","year":"2017","journal-title":"Front. Immunol."}],"container-title":["Clinica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898122011780?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898122011780?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T15:06:16Z","timestamp":1757862376000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009898122011780"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":32,"alternative-id":["S0009898122011780"],"URL":"https:\/\/doi.org\/10.1016\/j.cca.2022.05.025","relation":{},"ISSN":["0009-8981"],"issn-type":[{"value":"0009-8981","type":"print"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"T-cell proliferation assay for the detection of SARS-CoV-2-specific T-cells","name":"articletitle","label":"Article Title"},{"value":"Clinica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cca.2022.05.025","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}