{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:45:53Z","timestamp":1790189153874,"version":"4.1.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007637","name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["125189783229"],"award-info":[{"award-number":["125189783229"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022965","name":"Ministerio de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["897\/2021"],"award-info":[{"award-number":["897\/2021"]}],"id":[{"id":"10.13039\/100022965","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinica Chimica Acta"],"published-print":{"date-parts":[[2025,2]]},"DOI":"10.1016\/j.cca.2025.120144","type":"journal-article","created":{"date-parts":[[2025,1,19]],"date-time":"2025-01-19T22:41:24Z","timestamp":1737326484000},"page":"120144","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Nanoparticle-based colorimetric assays for early and rapid screening of the oncogenic HPV variants 16 and 18"],"prefix":"10.1016","volume":"568","author":[{"given":"Carlos E.","family":"Navarro Chica","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie L.","family":"Alfonso Tob\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Jos\u00e9","family":"L\u00f3pez Abella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Paula","family":"Valencia Piedrahita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Neira Acevedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula C.","family":"Berm\u00fadez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Arrivillaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres","family":"Jaramillo-Botero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cca.2025.120144_b0005","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/S1470-2045(10)70230-8","article-title":"Human papillomavirus genotype attribution in invasive cervical cancer: a retrospective cross-sectional worldwide study","volume":"11","author":"de Sanjose","year":"2010","journal-title":"Lancet Oncol."},{"key":"10.1016\/j.cca.2025.120144_b0010","unstructured":"Cancers Linked With HPV Each Year | Cancer | CDC, (n.d.). https:\/\/www.cdc.gov\/cancer\/hpv\/cases.html?CDC_AAref_Val=https:\/\/www.cdc.gov\/cancer\/hpv\/statistics\/cases.htm (accessed October 27, 2024)."},{"key":"10.1016\/j.cca.2025.120144_b0015","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.aca.2020.08.030","article-title":"Applications of gold nanoparticles in ELISA, PCR, and immuno-PCR assays: A review","volume":"1143","author":"Tabatabaei","year":"2021","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.cca.2025.120144_b0020","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.ab.2004.06.004","article-title":"High-throughput polymerase chain reaction chemiluminescent enzyme immunoassay for typing and quantifying human papillomavirus DNAs","volume":"332","author":"Ambretti","year":"2004","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.cca.2025.120144_b0025","doi-asserted-by":"crossref","unstructured":"J. Verssimo, T.A.A. de Medeiros Fernandes, Human Papillomavirus: Biology and Pathogenesis, in: Human Papillomavirus and Related Diseases - From Bench to Bedside - A Clinical Perspective, InTech, 2012: pp. 3\u201340. https:\/\/doi.org\/10.5772\/27154.","DOI":"10.5772\/27154"},{"key":"10.1016\/j.cca.2025.120144_b0030","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1128\/MMBR.68.2.362-372.2004","article-title":"Pathogenesis of human papillomaviruses in differentiating epithelia","volume":"68","author":"Longworth","year":"2004","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.cca.2025.120144_b0035","doi-asserted-by":"crossref","first-page":"e01819","DOI":"10.1128\/jvi.01819-22","article-title":"Critical residues involved in the coassembly of L1 and L2 capsid proteins of human papillomavirus 16","volume":"97","author":"Chen","year":"2023","journal-title":"J. Virol."},{"key":"10.1016\/j.cca.2025.120144_b0040","doi-asserted-by":"crossref","DOI":"10.1149\/1945-7111\/ac2972","article-title":"Review\u2014bio-nanosensors: fundamentals and recent applications","volume":"168","author":"Perdomo","year":"2021","journal-title":"J. Electrochem. Soc."},{"key":"10.1016\/j.cca.2025.120144_b0045","first-page":"1","article-title":"Recent advances in colorimetric sensors based on gold nanoparticles for pathogen detection","volume":"13","author":"Yang","year":"2023","journal-title":"Biosensors (Basel)"},{"key":"10.1016\/j.cca.2025.120144_b0050","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1111\/j.1600-0714.2009.00799.x","article-title":"Use of quantum dots to detect human papillomavirus in oral squamous cell carcinoma","volume":"38","author":"Xue","year":"2009","journal-title":"J. Oral Pathol. Med."},{"key":"10.1016\/j.cca.2025.120144_b0055","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1007\/s00216-020-02879-z","article-title":"Reduced graphene oxide-based field effect transistors for the detection of E7 protein of human papillomavirus in saliva","volume":"413","author":"Aspermair","year":"2021","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.cca.2025.120144_b0060","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1016\/j.bios.2016.07.066","article-title":"Magnetic nanowires for rapid and ultrasensitive isolation of DNA from cervical specimens for the detection of multiple human papillomaviruses genotypes","volume":"86","author":"Lee","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.cca.2025.120144_b0065","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1007\/s00216-007-1768-z","article-title":"Gold nanoparticles for the development of clinical diagnosis methods","volume":"391","author":"Baptista","year":"2008","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.cca.2025.120144_b0070","doi-asserted-by":"crossref","first-page":"44307","DOI":"10.1021\/acsami.7b13873","article-title":"DNA modulates the interaction of genetically engineered DNA-binding proteins and gold nanoparticles: diagnosis of high-risk HPV infection","volume":"9","author":"Mao","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.cca.2025.120144_b0075","doi-asserted-by":"crossref","first-page":"5737","DOI":"10.1039\/D0NA00686F","article-title":"Development of label-free gold nanoparticle based rapid colorimetric assay for clinical\/point-of-care screening of cervical cancer","volume":"2","author":"Appidi","year":"2020","journal-title":"Nanoscale Adv."},{"key":"10.1016\/j.cca.2025.120144_b0080","unstructured":"H. Guti\u00e9rrez Pulido, R. de la Vara Salazar, An\u00e1lisis y dise\u00f1o de experimentos, Segunda, McGRAW-HILL\/INTERAMERICANA, 2008."},{"key":"10.1016\/j.cca.2025.120144_b0085","doi-asserted-by":"crossref","first-page":"224","DOI":"10.14356\/kona.2020011","article-title":"Synthesis of precision gold nanoparticles using Turkevich method","volume":"37","author":"Dong","year":"2020","journal-title":"Kona Powder Part. J."},{"key":"10.1016\/j.cca.2025.120144_b0090","unstructured":"R. Quattrocchi, Oscar; Albelaira, Sara; Laba, Introducci\u00f3n a la HPLC Aplicaci\u00f3n y Pr\u00e1ctica, Artes Graficas Farro S.A., 1992."},{"issue":"Suppl 1","key":"10.1016\/j.cca.2025.120144_b0095","first-page":"S49","article-title":"Limit of blank, limit of detection and limit of quantitation","volume":"29","author":"Armbruster","year":"2008","journal-title":"The clinical biochemist reviews"},{"key":"10.1016\/j.cca.2025.120144_b0100","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/nano11123370","article-title":"Analytical performance and validation of a reliable method based on graphite furnace atomic absorption spectrometry for the determination of gold nanoparticles in biological tissues","volume":"11","author":"Cadar","year":"2021","journal-title":"Nanomaterials"},{"key":"10.1016\/j.cca.2025.120144_b0105","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1007\/s10096-012-1602-1","article-title":"Methods and recommendations for evaluating and reporting a new diagnostic test","volume":"31","author":"Hess","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.cca.2025.120144_b0110","first-page":"1","article-title":"Foundational statistical principles in medical research: Sensitivity, specificity, positive predictive value, and negative predictive value","volume":"57","author":"Monaghan","year":"2021","journal-title":"Medicina (Lithuania)"},{"key":"10.1016\/j.cca.2025.120144_b0115","unstructured":"WHO recommends DNA testing as a first-choice screening method for cervical cancer prevention, (n.d.). https:\/\/www.who.int\/europe\/news\/item\/11-09-2021-who-recommends-dna-testing-as-a-first-choice-screening-method-for-cervical-cancer-prevention (accessed October 28, 2024)."},{"key":"10.1016\/j.cca.2025.120144_b0120","doi-asserted-by":"crossref","first-page":"S25","DOI":"10.1016\/S1386-6532(09)70005-8","article-title":"High-risk HPV detection and concurrent HPV 16 and 18 typing with Abbott RealTime High Risk HPV test","volume":"45","author":"Tang","year":"2009","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.cca.2025.120144_b0125","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00604-018-3065-2","article-title":"Colorimetric human papillomavirus DNA assay based on the retardation of avidin-induced aggregation of gold nanoparticles","volume":"185","author":"Piao","year":"2018","journal-title":"Microchim. Acta"},{"key":"10.1016\/j.cca.2025.120144_b0130","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/B978-0-323-88450-1.00005-3","article-title":"Methods for nanoparticle synthesis and drug delivery","author":"Choudhury","year":"2022","journal-title":"Adv. Nanotechnol.-Based Drug Deliv. Syst."},{"key":"10.1016\/j.cca.2025.120144_b0135","doi-asserted-by":"crossref","first-page":"6471","DOI":"10.1021\/acsanm.1c00987","article-title":"Nanoparticle size effects in biomedical applications","volume":"4","author":"Dolai","year":"2021","journal-title":"ACS Appl. Nano Mater."},{"key":"10.1016\/j.cca.2025.120144_b0140","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.1039\/C9NR08877F","article-title":"Mechanistic insights of the reduction of gold salts in the Turkevich protocol","volume":"12","author":"Gao","year":"2020","journal-title":"Nanoscale"},{"key":"10.1016\/j.cca.2025.120144_b0145","doi-asserted-by":"crossref","first-page":"18568","DOI":"10.1021\/jp0743119","article-title":"Surface charge density of maghemite nanoparticles: role of electrostatics in the proton exchange","volume":"111","author":"Lucas","year":"2007","journal-title":"J. Phys. Chem. C"},{"key":"10.1016\/j.cca.2025.120144_b0150","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/430_2013_138","article-title":"Historical introduction to gold colloids, clusters and nanoparticles","volume":"161","author":"Mingos","year":"2014","journal-title":"Struct Bond"},{"key":"10.1016\/j.cca.2025.120144_b0155","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jcis.2015.04.043","article-title":"Surface activity and flocculation behavior of polyethylene glycol-functionalized silica nanoparticles","volume":"452","author":"Bj\u00f6rkegren","year":"2015","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.cca.2025.120144_b0160","doi-asserted-by":"crossref","first-page":"6018","DOI":"10.1021\/la3051093","article-title":"Dissecting colloidal stabilization factors in crowded polymer solutions by forming self-assembled monolayers on gold nanoparticles","volume":"29","author":"Lang","year":"2013","journal-title":"Langmuir"},{"key":"10.1016\/j.cca.2025.120144_b0165","series-title":"J Phys Conf Ser","first-page":"1","article-title":"Epitope mapping of capsid protein l1 from human papillomavirus to development cervical cancer vaccine through computational study","author":"Purnama","year":"2018"},{"key":"10.1016\/j.cca.2025.120144_b0170","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1743-422X-5-150","article-title":"Evolutionary and structural analyses of alpha-papillomavirus capsid proteins yields novel insights into L2 structure and interaction with L1","volume":"5","author":"Lowe","year":"2008","journal-title":"Virol. J."},{"key":"10.1016\/j.cca.2025.120144_b0175","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1128\/JCM.36.7.2046-2051.1998","article-title":"The L1 major capsid protein of human papillomavirus type 16 variants affects yield of virus-like particles produced in an insect cell expression system","volume":"36","author":"Touze","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.cca.2025.120144_b0180","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0889-857X(02)00083-2","article-title":"Applications of polymerase chain reaction in rheumatology","volume":"29","author":"Cuchacovich","year":"2003","journal-title":"Rheum. Dis. Clin. N. Am."},{"key":"10.1016\/j.cca.2025.120144_b0185","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/j.1365-2672.1990.tb01512.x","article-title":"Preliminary development of a diagnostic test for Brucella using polymerase chain reaction","volume":"69","author":"Fekete","year":"1990","journal-title":"J. Appl. Bacteriol."},{"key":"10.1016\/j.cca.2025.120144_b0190","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/B978-012214730-2\/50015-7","article-title":"Fluorescence immunoassays","author":"Christopoulos","year":"1996","journal-title":"Immunoassay"},{"key":"10.1016\/j.cca.2025.120144_b0195","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.gocm.2023.01.001","article-title":"Clinical significance of extended high-risk human papillomavirus genotyping and viral load in cervical cancer and precancerous lesions","volume":"3","author":"Su","year":"2023","journal-title":"Gynecol. Obstetrics Clin. Med."},{"key":"10.1016\/j.cca.2025.120144_b0200","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1002\/cncr.23704","article-title":"Human papillomavirus infection and the primary and secondary prevention of cervical cancer","volume":"113","author":"Lowy","year":"2008","journal-title":"Cancer"}],"container-title":["Clinica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898125000233?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898125000233?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T07:44:31Z","timestamp":1740383071000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009898125000233"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":40,"alternative-id":["S0009898125000233"],"URL":"https:\/\/doi.org\/10.1016\/j.cca.2025.120144","relation":{},"ISSN":["0009-8981"],"issn-type":[{"value":"0009-8981","type":"print"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Nanoparticle-based colorimetric assays for early and rapid screening of the oncogenic HPV variants 16 and 18","name":"articletitle","label":"Article Title"},{"value":"Clinica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cca.2025.120144","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"120144"}}