{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T08:39:14Z","timestamp":1769848754543,"version":"3.49.0"},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinica Chimica Acta"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.cca.2025.120703","type":"journal-article","created":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T00:24:25Z","timestamp":1762561465000},"page":"120703","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Evaluation of an enzymatic one-step assay for Beta-Hydroxybutyrate blood testing on clinical chemistry platforms using HILIC-MS\/MS as a non-enzymatic reference method"],"prefix":"10.1016","volume":"580","author":[{"given":"Max K.","family":"Steinbach","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holger","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanik","family":"Reichard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantin","family":"Eulberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Fr\u00f6mel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Lein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Peter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Prager","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Grimmler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.cca.2025.120703_bb0005","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1146\/annurev-nutr-111120-111518","article-title":"Metabolic and signaling roles of ketone bodies in health and disease","volume":"41","author":"Puchalska","year":"2021","journal-title":"Annu. Rev. Nutr."},{"key":"10.1016\/j.cca.2025.120703_bb0010","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1146\/annurev-nutr-071816-064916","article-title":"beta-Hydroxybutyrate: a signaling metabolite","volume":"37","author":"Newman","year":"2017","journal-title":"Annu. Rev. Nutr."},{"key":"10.1016\/j.cca.2025.120703_bb0015","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1177\/19322968231152236","article-title":"Update on measuring ketones","volume":"18","author":"Huang","year":"2024","journal-title":"J. Diabetes Sci. Technol."},{"key":"10.1016\/j.cca.2025.120703_bb0020","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s13643-023-02203-7","article-title":"What is the utility of blood beta-hydroxybutyrate measurements in emergency department in patients without diabetes: a systematic review","volume":"12","author":"Hui","year":"2023","journal-title":"Syst. Rev."},{"key":"10.1016\/j.cca.2025.120703_bb0025","doi-asserted-by":"crossref","first-page":"2802","DOI":"10.1097\/MS9.0000000000000894","article-title":"Comprehensive review of diabetic ketoacidosis: an update","volume":"85","author":"Elendu","year":"2023","journal-title":"Ann. Med. Surg. (Lond)."},{"key":"10.1016\/j.cca.2025.120703_bb0030","first-page":"110","article-title":"Severe diabetic ketoacidosis in children with Type 1 diabetes: ongoing challenges in care","volume":"12","author":"Foti Randazzese","year":"2025","journal-title":"Children (Basel)"},{"key":"10.1016\/j.cca.2025.120703_bb0035","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.2337\/dc18-2316","article-title":"International consensus on risk Management of Diabetic Ketoacidosis in patients with type 1 diabetes treated with sodium-glucose cotransporter (SGLT) inhibitors","volume":"42","author":"Danne","year":"2019","journal-title":"Diabetes Care"},{"key":"10.1016\/j.cca.2025.120703_bb0040","doi-asserted-by":"crossref","DOI":"10.1002\/dmrr.2886","article-title":"Ketosis and diabetic ketoacidosis in response to SGLT2 inhibitors: basic mechanisms and therapeutic perspectives","volume":"33","author":"Qiu","year":"2017","journal-title":"Diabetes Metab. Res. Rev."},{"key":"10.1016\/j.cca.2025.120703_bb0045","doi-asserted-by":"crossref","DOI":"10.1111\/dme.14788","article-title":"The management of diabetic ketoacidosis in adults-an updated guideline from the Joint British Diabetes Society for Inpatient Care","volume":"39","author":"Dhatariya","year":"2022","journal-title":"Diabet. Med."},{"key":"10.1016\/j.cca.2025.120703_bb0050","doi-asserted-by":"crossref","first-page":"2609","DOI":"10.1007\/s00125-021-05568-3","article-title":"The management of type 1 diabetes in adults. A consensus report by the American Diabetes Association (ADA) and the European Association for the Study of Diabetes (EASD)","volume":"64","author":"Holt","year":"2021","journal-title":"Diabetologia"},{"key":"10.1016\/j.cca.2025.120703_bb0055","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.2337\/dci24-0032","article-title":"Hyperglycemic crises in adults with diabetes: a consensus report","volume":"47","author":"Umpierrez","year":"2024","journal-title":"Diabetes Care"},{"key":"10.1016\/j.cca.2025.120703_bb0060","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1111\/1556-4029.14443","article-title":"Utility of postmortem vitreous Beta-Hydroxybutyrate testing for distinguishing sudden from prolonged deaths and for diagnosing ketoacidosis","volume":"65","author":"Klaric","year":"2020","journal-title":"J. Forensic Sci."},{"key":"10.1016\/j.cca.2025.120703_bb0065","doi-asserted-by":"crossref","DOI":"10.3390\/biom12010009","article-title":"The importance of BHB testing on the post-mortem diagnosis of ketoacidosis","volume":"12","author":"Ahlstrom","year":"2021","journal-title":"Biomolecules"},{"key":"10.1016\/j.cca.2025.120703_bb0070","doi-asserted-by":"crossref","DOI":"10.3389\/fnins.2023.1297984","article-title":"Ketone bodies mediate alterations in brain energy metabolism and biomarkers of Alzheimer's disease","volume":"17","author":"Ramezani","year":"2023","journal-title":"Front. Neurosci."},{"key":"10.1016\/j.cca.2025.120703_bb0075","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.chembiol.2024.11.001","article-title":"Beta-hydroxybutyrate is a metabolic regulator of proteostasis in the aged and Alzheimer disease brain","volume":"32","author":"Madhavan","year":"2025","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.cca.2025.120703_bb0080","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1186\/s12944-024-02368-7","article-title":"Therapeutic potential of beta-hydroxybutyrate in the management of pancreatic neoplasms: exploring novel diagnostic and treatment strategies","volume":"23","author":"Vaezi","year":"2024","journal-title":"Lipids Health Dis."},{"key":"10.1016\/j.cca.2025.120703_bb0085","series-title":"CLSI EP05 \u2013 Evaluation of Precision of Quantitative Measurement Procedures \u2013 Third Edition","author":"Clinical and Laboratory Standards Institute","year":"2014"},{"key":"10.1016\/j.cca.2025.120703_bb0090","series-title":"CLSI EP07 \u2013 Interference Testing in Clinical Chemistry \u2013 Third Edition","author":"Clinical and Laboratory Standards Institute","year":"2018"},{"key":"10.1016\/j.cca.2025.120703_bb0095","series-title":"CLSI EP25 \u2013 Evaluation of Stability of in Vitro Medical Laboratory Test Reagents \u2013 Second Edition","author":"Clinical and Laboratory Standards Institute","year":"2023"},{"key":"10.1016\/j.cca.2025.120703_bb0100","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph13090837","article-title":"Evaluation of the accuracy of capillary Hydroxybutyrate measurement compared with other measurements in the diagnosis of diabetic ketoacidosis: a systematic review","volume":"13","author":"Brooke","year":"2016","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.cca.2025.120703_bb0105","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1177\/1932296814563351","article-title":"Comparative performance assessment of point-of-care testing devices for measuring glucose and ketones at the patient bedside","volume":"9","author":"Ceriotti","year":"2015","journal-title":"J. Diabetes Sci. Technol."},{"key":"10.1016\/j.cca.2025.120703_bb0110","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1093\/clinchem\/hvad020","article-title":"Establishing pragmatic analytical performance specifications for blood Beta-Hydroxybutyrate testing","volume":"69","author":"Kilpatrick","year":"2023","journal-title":"Clin. Chem."},{"key":"10.1016\/j.cca.2025.120703_bb0115","doi-asserted-by":"crossref","first-page":"e151","DOI":"10.2337\/dci23-0036","article-title":"Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes Mellitus","volume":"46","author":"Sacks","year":"2023","journal-title":"Diabetes Care"},{"key":"10.1016\/j.cca.2025.120703_bb0120","doi-asserted-by":"crossref","DOI":"10.11613\/BM.2020.010707","article-title":"Test validation, method comparison and reference range for the measurement of beta-hydroxybutyrate in peripheral blood samples","volume":"30","author":"Kraus","year":"2020","journal-title":"Biochem. Med. (Zagreb)."},{"key":"10.1016\/j.cca.2025.120703_bb0125","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.jmsacl.2024.01.004","article-title":"Development and validation of a multiplexed LC-MS\/MS ketone body assay for clinical diagnostics","volume":"31","author":"Kemperman","year":"2024","journal-title":"J. Mass. Spectrom. Adv. Clin. Lab."},{"key":"10.1016\/j.cca.2025.120703_bb0130","doi-asserted-by":"crossref","first-page":"267","DOI":"10.2337\/dc21-2279","article-title":"Controversies around the measurement of blood ketones to diagnose and manage diabetic ketoacidosis","volume":"45","author":"Kilpatrick","year":"2022","journal-title":"Diabetes Care"}],"container-title":["Clinica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898125005820?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898125005820?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T17:58:44Z","timestamp":1769795924000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009898125005820"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":26,"alternative-id":["S0009898125005820"],"URL":"https:\/\/doi.org\/10.1016\/j.cca.2025.120703","relation":{},"ISSN":["0009-8981"],"issn-type":[{"value":"0009-8981","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluation of an enzymatic one-step assay for Beta-Hydroxybutyrate blood testing on clinical chemistry platforms using HILIC-MS\/MS as a non-enzymatic reference method","name":"articletitle","label":"Article Title"},{"value":"Clinica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cca.2025.120703","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"120703"}}