{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T03:58:53Z","timestamp":1785297533561,"version":"3.55.0"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinica Chimica Acta"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.cca.2026.120986","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T08:01:46Z","timestamp":1774512106000},"page":"120986","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["High-throughput quantification of tacrolimus in whole blood by acoustic ejection mass spectrometry: analytical validation and comparative assessment against conventional LC-MS\/MS for therapeutic drug monitoring"],"prefix":"10.1016","volume":"588","author":[{"given":"Xin-Yu","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Kang","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Peng","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan-Fei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Ting","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Qi","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"26","key":"10.1016\/j.cca.2026.120986_bb0005","doi-asserted-by":"crossref","first-page":"2678","DOI":"10.1056\/NEJMp048256","article-title":"Transplantation\u2014a medical miracle of the 20th century","volume":"351","author":"Morris","year":"2004","journal-title":"N. Engl. J. Med."},{"issue":"3","key":"10.1016\/j.cca.2026.120986_bb0010","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1586\/1744666X.2016.1123093","article-title":"Tacrolimus for the prevention and treatment of rejection of solid organ transplants","volume":"12","author":"Scalea","year":"2016","journal-title":"Expert Rev. Clin. Immunol."},{"issue":"6","key":"10.1016\/j.cca.2026.120986_bb0015","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1111\/cts.12833","article-title":"Fasting status and circadian variation must be considered when performing AUC-based therapeutic drug monitoring of tacrolimus in renal transplant recipients","volume":"13","author":"Gustavsen","year":"2020","journal-title":"Clin. Transl. Sci."},{"issue":"8","key":"10.1016\/j.cca.2026.120986_bb0020","first-page":"547","article-title":"Tacrolimus, cytochrome P450, interactions with food variables in organ transplant recipients; a current and comprehensive review","volume":"25","author":"Zahra","year":"2024","journal-title":"Curr. Drug Metab."},{"issue":"7","key":"10.1016\/j.cca.2026.120986_bb0025","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.3390\/jcm9072193","article-title":"Impacts of high intra- and inter-individual variability in tacrolimus pharmacokinetics and fast tacrolimus metabolism on outcomes of solid organ transplant recipients","volume":"9","author":"Thongprayoon","year":"2020","journal-title":"J. Clin. Med."},{"key":"10.1016\/j.cca.2026.120986_bb0030","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cca.2015.07.022","article-title":"A clinical approach to solving discrepancies in therapeutic drug monitoring results for patients on sirolimus or tacrolimus: towards personalized medicine, immunosuppression and pharmacogenomics","volume":"450","author":"Millner","year":"2015","journal-title":"Clin. Chim. Acta"},{"issue":"7","key":"10.1016\/j.cca.2026.120986_bb0035","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1111\/ajt.13691","article-title":"A randomized controlled trial comparing the efficacy of Cyp3a5 genotype\u2010based with body\u2010weight\u2010based tacrolimus dosing after living donor kidney transplantation","volume":"16","author":"Shuker","year":"2016","journal-title":"Am. J. Transplant."},{"issue":"2","key":"10.1016\/j.cca.2026.120986_bb0040","doi-asserted-by":"crossref","DOI":"10.1016\/j.trre.2021.100610","article-title":"Identifying the association between tacrolimus exposure and toxicity in heart and lung transplant recipients: a systematic review","volume":"35","author":"Braithwaite","year":"2021","journal-title":"Transplant. Rev."},{"key":"10.1016\/j.cca.2026.120986_bb0045","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.cca.2012.07.018","article-title":"Impact of CYP3A5 genetic polymorphism on cross-reactivity in tacrolimus chemiluminescent immunoassay in kidney transplant recipients","volume":"414","author":"Hirano","year":"2012","journal-title":"Clin. Chim. Acta"},{"issue":"1","key":"10.1016\/j.cca.2026.120986_bb0050","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1097\/01.tp.0000188118.34633.bf","article-title":"Influence of CYP3A5 gene polymorphisms of donor rather than recipient to tacrolimus individual dose requirement in liver transplantation","volume":"81","author":"Yu","year":"2006","journal-title":"Transplantation"},{"issue":"6","key":"10.1016\/j.cca.2026.120986_bb0055","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1093\/clinchem\/48.6.955","article-title":"Rapid liquid chromatography\u2013tandem mass spectrometry routine method for simultaneous determination of sirolimus, everolimus, tacrolimus, and cyclosporin a in whole blood","volume":"48","author":"Streit","year":"2002","journal-title":"Clin. Chem."},{"issue":"1","key":"10.1016\/j.cca.2026.120986_bb0060","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/s43586-022-00175-x","article-title":"Liquid chromatography\u2013tandem mass spectrometry for clinical diagnostics","volume":"2","author":"Thomas","year":"2022","journal-title":"Nat. Rev. Methods Primers"},{"issue":"15\u201316","key":"10.1016\/j.cca.2026.120986_bb0065","article-title":"Determination of tacrolimus in human whole blood in kidney transplant recipients using a rapid and specific LC\u2013MS\/MS method","volume":"37","author":"Hieu","year":"2023","journal-title":"J. Clin. Lab. Anal."},{"key":"10.1016\/j.cca.2026.120986_bb0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.jchromb.2021.122698","article-title":"A simple and easy-to-perform liquid chromatography\u2013mass spectrometry method for the quantification of tacrolimus and its metabolites in human whole blood. Application to the determination of metabolic ratios in kidney transplant patients","volume":"1173","author":"Chavant","year":"2021","journal-title":"J. Chromatogr. B"},{"issue":"8","key":"10.1016\/j.cca.2026.120986_bb0075","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2022.e10214","article-title":"A modified LC-MS\/MS method for the detection of whole blood tacrolimus and its clinical value in Chinese kidney transplant patients","volume":"8","author":"Yu","year":"2022","journal-title":"Heliyon"},{"issue":"31","key":"10.1016\/j.cca.2026.120986_bb0080","doi-asserted-by":"crossref","first-page":"10850","DOI":"10.1021\/acs.analchem.1c01137","article-title":"Acoustic ejection mass spectrometry for high-throughput analysis","volume":"93","author":"Zhang","year":"2021","journal-title":"Anal. Chem."},{"issue":"41","key":"10.1016\/j.cca.2026.120986_bb0085","doi-asserted-by":"crossref","first-page":"22457","DOI":"10.1021\/acs.analchem.5c02331","article-title":"Twenty-five years of high-throughput screening of biological samples with mass spectrometry: current platforms and emerging methods","volume":"97","author":"Smith","year":"2025","journal-title":"Anal. Chem."},{"issue":"6","key":"10.1016\/j.cca.2026.120986_bb0090","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1021\/acsmedchemlett.0c00066","article-title":"Ultra-high-throughput acoustic droplet ejection-open port Interface-mass spectrometry for parallel medicinal chemistry","volume":"11","author":"DiRico","year":"2020","journal-title":"ACS Med. Chem. Lett."},{"issue":"7","key":"10.1016\/j.cca.2026.120986_bb0095","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1080\/17460441.2022.2084069","article-title":"Acoustic ejection mass spectrometry: fundamentals and applications in high-throughput drug discovery","volume":"17","author":"Liu","year":"2022","journal-title":"Expert Opin. Drug Discov."},{"issue":"5","key":"10.1016\/j.cca.2026.120986_bb0100","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.slasd.2023.04.001","article-title":"Label-free high-throughput screening via acoustic ejection mass spectrometry put into practice","volume":"28","author":"Winter","year":"2023","journal-title":"SLAS Discovery"},{"issue":"1","key":"10.1016\/j.cca.2026.120986_bb0105","doi-asserted-by":"crossref","first-page":"5114","DOI":"10.1038\/s41467-024-48563-z","article-title":"Acoustic ejection mass spectrometry empowers ultra-fast protein biomarker quantification","volume":"15","author":"Van Puyvelde","year":"2024","journal-title":"Nat. Commun."},{"issue":"18","key":"10.1016\/j.cca.2026.120986_bb0110","doi-asserted-by":"crossref","first-page":"9613","DOI":"10.1021\/acs.analchem.5c01004","article-title":"Derivatization-assisted acoustic ejection mass spectrometry for high-throughput FAHFA prescreening","volume":"97","author":"An","year":"2025","journal-title":"Anal. Chem."},{"issue":"3","key":"10.1016\/j.cca.2026.120986_bb0115","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1021\/acs.analchem.3c04211","article-title":"Accelerating pharmaceutical process development with an acoustic droplet ejection-multiple reaction monitoring-mass spectrometry workflow","volume":"96","author":"Hu","year":"2024","journal-title":"Anal. Chem."},{"issue":"2","key":"10.1016\/j.cca.2026.120986_bb0120","article-title":"Application of acoustic ejection mass spectrometry to accelerate drug discovery","volume":"6","author":"Meng","year":"2026","journal-title":"Anal. Sens."},{"issue":"1","key":"10.1016\/j.cca.2026.120986_bb0125","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1373\/clinchem.2015.238626","article-title":"CLSI C62-a: a new standard for clinical mass spectrometry","volume":"62","author":"Lynch","year":"2016","journal-title":"Clin. Chem."},{"key":"10.1016\/j.cca.2026.120986_bb0130","author":"European Medicines Agency (EMA), ICH guideline M10 on bioanalytical method validation"},{"issue":"2","key":"10.1016\/j.cca.2026.120986_bb0135","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1093\/clinchem\/42.2.279","article-title":"Sensitive, specific quantitative analysis of tacrolimus (FK506) in blood by liquid chromatography-electrospray tandem mass spectrometry","volume":"42","author":"Taylor","year":"1996","journal-title":"Clin. Chem."},{"issue":"13","key":"10.1016\/j.cca.2026.120986_bb0140","doi-asserted-by":"crossref","first-page":"3019","DOI":"10.1021\/ac020361s","article-title":"Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC\u2212MS\/MS","volume":"75","author":"Matuszewski","year":"2003","journal-title":"Anal. Chem."}],"container-title":["Clinica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898126001683?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898126001683?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T00:50:19Z","timestamp":1778892619000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009898126001683"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":28,"alternative-id":["S0009898126001683"],"URL":"https:\/\/doi.org\/10.1016\/j.cca.2026.120986","relation":{},"ISSN":["0009-8981"],"issn-type":[{"value":"0009-8981","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High-throughput quantification of tacrolimus in whole blood by acoustic ejection mass spectrometry: analytical validation and comparative assessment against conventional LC-MS\/MS for therapeutic drug monitoring","name":"articletitle","label":"Article Title"},{"value":"Clinica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cca.2026.120986","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"120986"}}