{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:59:15Z","timestamp":1792083555607,"version":"4.4.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinica Chimica Acta"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.cca.2026.121269","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T15:01:56Z","timestamp":1786201316000},"page":"121269","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Lateral flow immunoassays: challenges and innovations"],"prefix":"10.1016","volume":"593","author":[{"given":"Kranti","family":"Patil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanti","family":"Upadhye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shrimati","family":"Shetty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cca.2026.121269_bb0005","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1042\/EBC20150012","article-title":"Review of lateral flow assays","volume":"60","author":"Koczula","year":"2016","journal-title":"Essays Biochem."},{"key":"10.1016\/j.cca.2026.121269_bb0010","series-title":"Methods Mol. Biol.","first-page":"1","article-title":"Evolution in Lateral Flow\u2013Based Immunoassay Systems","author":"O\u2019Farrell","year":"2008"},{"key":"10.1016\/j.cca.2026.121269_bb0015","doi-asserted-by":"crossref","first-page":"280","DOI":"10.3934\/microbiol.2020018","article-title":"Six decades of lateral flow immunoassay: from determining metabolic markers to diagnosing COVID-19","volume":"6","author":"Andryukov","year":"2020","journal-title":"AIMS Microbiol"},{"key":"10.1016\/j.cca.2026.121269_bb0020","doi-asserted-by":"crossref","first-page":"1970","DOI":"10.1039\/C8AN00307F","article-title":"Recent advances in nanoparticle-based lateral flow immunoassay as a point-of-care diagnostic tool for infectious agents and diseases","volume":"143","author":"Banerjee","year":"2018","journal-title":"Analyst"},{"key":"10.1016\/j.cca.2026.121269_bb0025","doi-asserted-by":"crossref","first-page":"354","DOI":"10.3390\/bios13030354","article-title":"Development of gold-nanoparticle-based lateral flow immunoassays for rapid detection of TB ESAT-6 and CFP-10","volume":"13","author":"Seele","year":"2023","journal-title":"Biosensors"},{"key":"10.1016\/j.cca.2026.121269_bb0030","doi-asserted-by":"crossref","first-page":"3788","DOI":"10.1038\/s41596-020-0357-x","article-title":"Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays","volume":"15","author":"Parolo","year":"2020","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.cca.2026.121269_bb0035","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.trac.2016.06.006","article-title":"Lateral flow assays: principles, designs and labels","volume":"82","author":"Bahad\u0131r","year":"2016","journal-title":"TrAC Trends Anal. Chem."},{"key":"10.1016\/j.cca.2026.121269_bb0040","doi-asserted-by":"crossref","first-page":"5185","DOI":"10.3390\/s21155185","article-title":"Ten years of lateral flow immunoassay technique applications: trends, challenges and future perspectives","volume":"21","author":"Di Nardo","year":"2021","journal-title":"Sensors"},{"key":"10.1016\/j.cca.2026.121269_bb0045","doi-asserted-by":"crossref","first-page":"837","DOI":"10.3390\/bios13090837","article-title":"Lateral flow assay: a summary of recent progress for improving assay performance","volume":"13","author":"Omidfar","year":"2023","journal-title":"Biosensors"},{"key":"10.1016\/j.cca.2026.121269_bb0050","article-title":"Lateral flow assays: progress and evolution of recent trends in point-of-care applications","volume":"28","author":"Kakkar","year":"2024","journal-title":"Mater. Today Bio"},{"key":"10.1016\/j.cca.2026.121269_bb0055","doi-asserted-by":"crossref","first-page":"3593","DOI":"10.1021\/acsnano.0c10035","article-title":"Ultrasensitive and highly specific lateral flow assays for point-of-care diagnosis","volume":"15","author":"Liu","year":"2021","journal-title":"ACS Nano"},{"key":"10.1016\/j.cca.2026.121269_bb0060","doi-asserted-by":"crossref","first-page":"2486","DOI":"10.1039\/C9LC00104B","article-title":"Sensitivity enhancement in lateral flow assays: a systems perspective","volume":"19","author":"Bishop","year":"2019","journal-title":"Lab Chip"},{"key":"10.1016\/j.cca.2026.121269_bb0065","doi-asserted-by":"crossref","first-page":"4351","DOI":"10.1039\/D3AY00844D","article-title":"New technologies and reagents in lateral flow assay (LFA) designs for enhancing accuracy and sensitivity","volume":"15","author":"Dey","year":"2023","journal-title":"Anal. Methods"},{"key":"10.1016\/j.cca.2026.121269_bb0070","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-35694-9","article-title":"Development a stacking pad design for enhancing the sensitivity of lateral flow immunoassay","volume":"8","author":"Tsai","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cca.2026.121269_bb0075","article-title":"Weakly ionized gold nanoparticles amplify immunoassays for ultrasensitive point-of-care sensors","volume":"10","author":"Zhang","year":"2024","journal-title":"Sci. Adv."},{"key":"10.1016\/j.cca.2026.121269_bb0080","doi-asserted-by":"crossref","first-page":"4111","DOI":"10.1007\/s00216-021-03421-5","article-title":"Strip modification and alternative architectures for signal amplification in nanoparticle-based lateral flow assays","volume":"413","author":"D\u00edaz-Gonz\u00e1lez","year":"2021","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.cca.2026.121269_bb0085","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1039\/D4LC01075B","article-title":"A comprehensive review of competitive lateral flow assays over the past decade","volume":"25","author":"Pedreira-Rinc\u00f3n","year":"2025","journal-title":"Lab Chip"},{"key":"10.1016\/j.cca.2026.121269_bb0090","first-page":"403","article-title":"A novel competitive ELISA for detection of antibodies against bovine viral diarrhea virus infection","volume":"13","author":"Seyfi Abad Shapouri","year":"2022","journal-title":"Vet. Res. Forum"},{"key":"10.1016\/j.cca.2026.121269_bb0095","doi-asserted-by":"crossref","first-page":"5083","DOI":"10.1021\/acsomega.8b03593","article-title":"Signal-enhanced lateral flow immunoassay with dual gold nanoparticle conjugates for the detection of hepatitis B surface antigen","volume":"4","author":"Shen","year":"2019","journal-title":"ACS Omega"},{"key":"10.1016\/j.cca.2026.121269_bb0100","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202505975","article-title":"Drug-tolerant, chemiluminescent lateral flow immunoassay platform for the determination of neutralizing anti-drug antibodies","volume":"21","author":"Kokojka","year":"2025","journal-title":"Small"},{"key":"10.1016\/j.cca.2026.121269_bb0105","doi-asserted-by":"crossref","first-page":"266","DOI":"10.3390\/bioengineering9070266","article-title":"Recent advances in silica-nanomaterial-assisted lateral flow assay","volume":"9","author":"Zhuang","year":"2022","journal-title":"Bioengineering"},{"key":"10.1016\/j.cca.2026.121269_bb0110","doi-asserted-by":"crossref","first-page":"4358","DOI":"10.1002\/anie.201200997","article-title":"Significantly improved analytical sensitivity of lateral flow immunoassays by using thermal contrast","volume":"51","author":"Qin","year":"2012","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.cca.2026.121269_bb0115","doi-asserted-by":"crossref","DOI":"10.3390\/bios11090295","article-title":"Recent advances in novel lateral flow technologies for detection of COVID-19","volume":"11","author":"Hsiao","year":"2021","journal-title":"Biosensors"},{"key":"10.1016\/j.cca.2026.121269_bb0120","doi-asserted-by":"crossref","first-page":"14881","DOI":"10.1021\/acs.chemrev.1c01012","article-title":"Toward next generation lateral flow assays: integration of nanomaterials","volume":"122","author":"Sena-Torralba","year":"2022","journal-title":"Chem. Rev."},{"key":"10.1016\/j.cca.2026.121269_bb0125","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3390\/bios9010002","article-title":"Multiplex lateral flow immunoassay: an overview of strategies towards high-throughput point-of-need testing","volume":"9","author":"Anfossi","year":"2018","journal-title":"Biosensors"},{"key":"10.1016\/j.cca.2026.121269_bb0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2021.128429","article-title":"Programmable microfluidic flow for automatic multistep digital assay in a single-sheet 3-dimensional paper-based microfluidic device","volume":"411","author":"Jeong","year":"2021","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.cca.2026.121269_bb0135","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1021\/acs.analchem.9b03792","article-title":"Transposing lateral flow immunoassays to capillary-driven microfluidics using self-coalescence modules and capillary-assembled receptor carriers","volume":"92","author":"Hemmig","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.cca.2026.121269_bb0140","article-title":"Scalable and parallelized biochemical assays in paper devices integrated with a programmable binary valve matrix","volume":"321","author":"Yuan","year":"2020","journal-title":"Sensors Actuators B Chem."},{"key":"10.1016\/j.cca.2026.121269_bb0145","doi-asserted-by":"crossref","first-page":"68","DOI":"10.3390\/bios15020068","article-title":"Beyond traditional lateral flow assays: enhancing performance through multianalytical strategies","volume":"15","author":"Lamprou","year":"2025","journal-title":"Biosensors"}],"container-title":["Clinica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898126004511?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009898126004511?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:27:44Z","timestamp":1792081664000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009898126004511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":29,"alternative-id":["S0009898126004511"],"URL":"https:\/\/doi.org\/10.1016\/j.cca.2026.121269","relation":{},"ISSN":["0009-8981"],"issn-type":[{"value":"0009-8981","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Lateral flow immunoassays: challenges and innovations","name":"articletitle","label":"Article Title"},{"value":"Clinica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cca.2026.121269","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"121269"}}