{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:02:10Z","timestamp":1789470130487,"version":"build-2803163510"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2022-03718"],"award-info":[{"award-number":["RGPIN-2022-03718"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cells &amp; Development"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.cdev.2026.204101","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T16:05:36Z","timestamp":1786723536000},"page":"204101","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Gestational inhibition of CSF1R signaling using PLX5622 drives musculoskeletal changes in postnatal offspring"],"prefix":"10.1016","volume":"187","author":[{"given":"Rouzbeh","family":"Ostadsharif Memar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Rosin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddharth R.","family":"Vora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica M.","family":"Rosin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.cdev.2026.204101_bb0005","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.1210\/endo.143.5.8775","article-title":"Rescue of the Osteopetrotic Defect in op\/op mice by osteoblast-specific targeting of soluble Colony-stimulating Factor-1","volume":"143","author":"Abboud","year":"2002","journal-title":"Endocrinology"},{"issue":"2","key":"10.1016\/j.cdev.2026.204101_bb0010","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1095\/biolreprod54.2.478","article-title":"Follicular development and ovulation in macrophage Colony-stimulating factor-deficient mice homozygous for the Osteopetrosis (Op) Mutation1","volume":"54","author":"Araki","year":"1996","journal-title":"Biol. Reprod."},{"issue":"2","key":"10.1016\/j.cdev.2026.204101_bb0015","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1189\/jlb.1103591","article-title":"Insulin cell mass is altered in Csf1 op \/Csf1 op macrophage-deficient mice","volume":"76","author":"Banaei-Bouchareb","year":"2004","journal-title":"J. Leukoc. Biol."},{"issue":"1","key":"10.1016\/j.cdev.2026.204101_bb0020","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1111\/joa.12059","article-title":"The morphology of the mouse masticatory musculature","volume":"223","author":"Baverstock","year":"2013","journal-title":"J. Anat."},{"issue":"1","key":"10.1016\/j.cdev.2026.204101_bb0025","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1084\/jem.177.1.237","article-title":"Delayed hematopoietic development in osteopetrotic (op\/op) mice","volume":"177","author":"Begg","year":"1993","journal-title":"J. Exp. Med."},{"issue":"1","key":"10.1016\/j.cdev.2026.204101_bb0030","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1006\/excr.1995.1149","article-title":"Colony-stimulating factor 1 (CSF-1)is involved in an autocrine growth control of rat myogenic cells","volume":"218","author":"Borycki","year":"1995","journal-title":"Exp. Cell Res."},{"issue":"6","key":"10.1016\/j.cdev.2026.204101_bb0035","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.tins.2016.03.005","article-title":"Emerging roles for CSF-1 receptor and its ligands in the nervous system","volume":"39","author":"Chitu","year":"2016","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.cdev.2026.204101_bb0040","article-title":"Homozygous mutation in CSF1R causes brain abnormalities, neurodegeneration, and dysosteosclerosis (BANDDOS)","volume":"1","author":"Daghagh","year":"2022","journal-title":"Bioimpacts"},{"issue":"1","key":"10.1016\/j.cdev.2026.204101_bb0045","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1182\/blood.V99.1.111","article-title":"Targeted disruption of the mouse colony-stimulating factor 1 receptor gene results in osteopetrosis, mononuclear phagocyte deficiency, increased primitive progenitor cell frequencies, and reproductive defects","volume":"99","author":"Dai","year":"2002","journal-title":"Blood"},{"issue":"9","key":"10.1016\/j.cdev.2026.204101_bb0050","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1359\/JBMR.040514","article-title":"Osteoclast deficiency results in disorganized matrix, reduced mineralization, and abnormal osteoblast behavior in developing bone","volume":"19","author":"Dai","year":"2004","journal-title":"J. Bone Miner. Res."},{"issue":"1","key":"10.1016\/j.cdev.2026.204101_bb0055","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1186\/s13023-023-02772-9","article-title":"Brain abnormalities, neurodegeneration, and dysosteosclerosis (BANDDOS): new cases, systematic literature review, and associations with CSF1R-ALSP","volume":"18","author":"Dulski","year":"2023","journal-title":"Orphanet J. Rare Dis."},{"issue":"2","key":"10.1016\/j.cdev.2026.204101_bb0060","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.ajpath.2012.10.010","article-title":"Macrophage colony-stimulating factor\u2013induced macrophage differentiation promotes regrowth in atrophied skeletal muscles and C2C12 myotubes","volume":"182","author":"Dumont","year":"2013","journal-title":"Am. J. Pathol."},{"issue":"10","key":"10.1016\/j.cdev.2026.204101_bb0065","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0026317","article-title":"Absence of colony stimulation factor-1 receptor results in loss of microglia, disrupted brain development and olfactory deficits. Meisel A, editor","volume":"6","author":"Erblich","year":"2011","journal-title":"PLoS One"},{"issue":"12","key":"10.1016\/j.cdev.2026.204101_bb0070","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1038\/s10038-021-00942-w","article-title":"From HDLS to BANDDOS: fast-expanding phenotypic spectrum of disorders caused by mutations in CSF1R","volume":"66","author":"Guo","year":"2021","journal-title":"J. Hum. Genet."},{"issue":"2","key":"10.1016\/j.cdev.2026.204101_bb0075","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1002\/JLB.MR0519-143R","article-title":"Phenotypic impacts of CSF1R deficiencies in humans and model organisms","volume":"107","author":"Hume","year":"2020","journal-title":"J. Leukoc. Biol."},{"issue":"S1","key":"10.1016\/j.cdev.2026.204101_bb0080","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/ocr.12304","article-title":"Analysis of facial skeletal asymmetry during foetal development using \u03bcCT imaging","volume":"22","author":"Katsube","year":"2019","journal-title":"Orthod. Craniofacial Res."},{"issue":"6","key":"10.1016\/j.cdev.2026.204101_bb0085","doi-asserted-by":"crossref","first-page":"e1009605","DOI":"10.1371\/journal.pgen.1009605","article-title":"CSF1R-dependent macrophages control postnatal somatic growth and organ maturation. Barsh GS, editor","volume":"17","author":"Keshvari","year":"2021","journal-title":"PLoS Genet."},{"issue":"9","key":"10.1016\/j.cdev.2026.204101_bb0090","doi-asserted-by":"crossref","DOI":"10.3390\/cells9092073","article-title":"Osteoblast-osteoclast communication and bone homeostasis","volume":"9","author":"Kim","year":"2020","journal-title":"Cells"},{"issue":"16","key":"10.1016\/j.cdev.2026.204101_bb0095","doi-asserted-by":"crossref","DOI":"10.1242\/dev.205423","article-title":"CSF1R+ macrophage and osteoclast depletion impairs neural crest proliferation and craniofacial morphogenesis","volume":"153","author":"Ma","year":"2026","journal-title":"Development"},{"issue":"1","key":"10.1016\/j.cdev.2026.204101_bb0100","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/oxfordjournals.jhered.a108657","article-title":"Osteopetrosis, a new recessive skeletal mutation on chromosome 12 of the mouse","volume":"67","author":"Marks","year":"1976","journal-title":"J. Hered."},{"issue":"3","key":"10.1016\/j.cdev.2026.204101_bb0105","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1002\/dvdy.21857","article-title":"MicroCT for developmental biology: a versatile tool for high\u2010contrast 3D imaging at histological resolutions","volume":"238","author":"Metscher","year":"2009","journal-title":"Dev. Dyn."},{"issue":"S1","key":"10.1016\/j.cdev.2026.204101_bb0110","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/ocr.12671","article-title":"Embryonic inhibition of colony\u2010stimulating factor 1 receptor impacts craniofacial morphogenesis","volume":"26","author":"Nagra","year":"2023","journal-title":"Orthod. Craniofacial Res."},{"issue":"5","key":"10.1016\/j.cdev.2026.204101_bb0115","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1016\/j.ajhg.2019.03.010","article-title":"Homozygous mutations in CSF1R cause a pediatric-onset leukoencephalopathy and can result in congenital absence of microglia","volume":"104","author":"Oosterhof","year":"2019","journal-title":"Am. J. Hum. Genet."},{"issue":"9","key":"10.1016\/j.cdev.2026.204101_bb0120","doi-asserted-by":"crossref","first-page":"2683","DOI":"10.4049\/jimmunol.1701783","article-title":"Pleiotropic impacts of macrophage and microglial deficiency on development in rats with targeted mutation of the Csf1r locus","volume":"201","author":"Pridans","year":"2018","journal-title":"J. Immunol."},{"key":"10.1016\/j.cdev.2026.204101_bb0125","series-title":"DeCA: A dense correspondence analysis toolkit for shape analysis. In: Wachinger C, Paniagua B, Elhabian S, Li J, Egger J, editors. Shape in Medical Imaging [Internet]","first-page":"259","author":"Rolfe","year":"2023"},{"key":"10.1016\/j.cdev.2026.204101_bb0130","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1016\/j.bbi.2018.07.023","article-title":"Depletion of embryonic microglia using the CSF1R inhibitor PLX5622 has adverse sex-specific effects on mice, including accelerated weight gain, hyperactivity and anxiolytic-like behaviour","volume":"73","author":"Rosin","year":"2018","journal-title":"Brain Behav. Immun."},{"issue":"9","key":"10.1016\/j.cdev.2026.204101_bb0135","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1016\/j.devcel.2021.03.018","article-title":"A subpopulation of embryonic microglia respond to maternal stress and influence nearby neural progenitors","volume":"56","author":"Rosin","year":"2021","journal-title":"Dev. Cell"},{"key":"10.1016\/j.cdev.2026.204101_bb0140","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.851556","article-title":"PLX5622 reduces disease severity in lethal CNS infection by off-target inhibition of peripheral inflammatory monocyte production","volume":"13","author":"Spiteri","year":"2022","journal-title":"Front. Immunol."},{"issue":"6","key":"10.1016\/j.cdev.2026.204101_bb0145","doi-asserted-by":"crossref","first-page":"a021857","DOI":"10.1101\/cshperspect.a021857","article-title":"CSF-1 receptor signaling in myeloid cells","volume":"6","author":"Stanley","year":"2014","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.cdev.2026.204101_bb0150","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2019.00250","article-title":"Developmental stage-specific distribution of macrophages in mouse mammary gland","volume":"7","author":"Stewart","year":"2019","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.cdev.2026.204101_bb0155","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2024.1349253","article-title":"The reciprocity of skeletal muscle and bone: an evolving view from mechanical coupling, secretory crosstalk to stem cell exchange","volume":"15","author":"Sui","year":"2024","journal-title":"Front. Physiol."},{"issue":"6","key":"10.1016\/j.cdev.2026.204101_bb0160","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1242\/dmm.007328","article-title":"Fetal overgrowth in the Cdkn1c mouse model of Beckwith-Wiedemann syndrome","volume":"4","author":"Tunster","year":"2011","journal-title":"Dis. Model. Mech."},{"issue":"22","key":"10.1016\/j.cdev.2026.204101_bb0165","doi-asserted-by":"crossref","first-page":"14303","DOI":"10.1073\/pnas.202332999","article-title":"The osteopetrotic mutation toothless (tl) is a loss-of-function frameshift mutation in the rat <i>Csf1<\/i> gene: evidence of a crucial role for CSF-1 in osteoclastogenesis and endochondral ossification","volume":"99","author":"Van Wesenbeeck","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cdev.2026.204101_bb0170","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2015.00417","article-title":"Postnatal ontogeny of the cranial base and craniofacial skeleton in male C57BL\/6J mice: a reference standard for quantitative analysis","volume":"6","author":"Vora","year":"2016","journal-title":"Front. Physiol."},{"issue":"34","key":"10.1016\/j.cdev.2026.204101_bb0175","doi-asserted-by":"crossref","first-page":"20729","DOI":"10.1073\/pnas.1915950117","article-title":"Heterogeneous origins and functions of mouse skeletal muscle-resident macrophages","volume":"117","author":"Wang","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"12","key":"10.1016\/j.cdev.2026.204101_bb0180","doi-asserted-by":"crossref","first-page":"4828","DOI":"10.1073\/pnas.87.12.4828","article-title":"Total absence of colony-stimulating factor 1 in the macrophage-deficient osteopetrotic (op\/op) mouse","volume":"87","author":"Wiktor-Jedrzejczak","year":"1990","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"5","key":"10.1016\/j.cdev.2026.204101_bb0185","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1073\/pnas.84.5.1268","article-title":"Purification of the colony-stimulating factor 1 receptor and demonstration of its tyrosine kinase activity","volume":"84","author":"Yeung","year":"1987","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Cells &amp; Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2667290126000318?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2667290126000318?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:02:35Z","timestamp":1789466555000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2667290126000318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":37,"alternative-id":["S2667290126000318"],"URL":"https:\/\/doi.org\/10.1016\/j.cdev.2026.204101","relation":{},"ISSN":["2667-2901"],"issn-type":[{"value":"2667-2901","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Gestational inhibition of CSF1R signaling using PLX5622 drives musculoskeletal changes in postnatal offspring","name":"articletitle","label":"Article Title"},{"value":"Cells & Development","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cdev.2026.204101","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"204101"}}