{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:47:17Z","timestamp":1792291637484,"version":"4.4.0"},"reference-count":38,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,5,19]],"date-time":"2017-05-19T00:00:00Z","timestamp":1495152000000},"content-version":"vor","delay-in-days":383,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell"],"published-print":{"date-parts":[[2016,5]]},"DOI":"10.1016\/j.cell.2016.04.032","type":"journal-article","created":{"date-parts":[[2016,4,30]],"date-time":"2016-04-30T06:39:19Z","timestamp":1461998359000},"page":"1238-1254","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2092,"title":["Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure"],"prefix":"10.1016","volume":"165","author":[{"given":"Xuyu","family":"Qian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha\u00a0Nam","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxi\u00a0M.","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Hadiono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah\u00a0C.","family":"Ogden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christy","family":"Hammack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory\u00a0R.","family":"Hamersky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadi","family":"Jacob","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki-jun","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Jeang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jai","family":"Thakor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel\u00a0A.","family":"Berg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunchai","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Chickering","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Nauen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Ying","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhexing","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly\u00a0M.","family":"Christian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Yong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brady\u00a0J.","family":"Maher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengli","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjun","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-li","family":"Ming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cell.2016.04.032_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.chom.2016.03.008","article-title":"Type III interferons produced by human placental trophoblasts confer protection against Zika virus infection","author":"Bayer","year":"2016","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.cell.2016.04.032_bib2","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.tins.2004.05.007","article-title":"Nature and nurture in brain development","volume":"27","author":"Ben-Ari","year":"2004","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.cell.2016.04.032_bib3","doi-asserted-by":"crossref","first-page":"14925","DOI":"10.1523\/JNEUROSCI.4499-10.2010","article-title":"Molecular pathways controlling development of thalamus and hypothalamus: from neural specification to circuit formation","volume":"30","author":"Blackshaw","year":"2010","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.cell.2016.04.032_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/S1473-3099(16)00095-5","article-title":"Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study","author":"Calvet","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.cell.2016.04.032_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/S0140-6736(16)00651-6","article-title":"Association between Zika virus and microcephaly in French Polynesia, 2013-15: a retrospective study","author":"Cauchemez","year":"2016","journal-title":"Lancet"},{"key":"10.1016\/j.cell.2016.04.032_bib6","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.mcn.2004.09.008","article-title":"The homeodomain transcription factor Pitx3 facilitates differentiation of mouse embryonic stem cells into AHD2-expressing dopaminergic neurons","volume":"28","author":"Chung","year":"2005","journal-title":"Mol. Cell. Neurosci."},{"key":"10.1016\/j.cell.2016.04.032_bib7","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1523\/JNEUROSCI.5128-10.2011","article-title":"Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals","volume":"31","author":"Danjo","year":"2011","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.cell.2016.04.032_bib8","doi-asserted-by":"crossref","DOI":"10.1056\/NEJMoa1601824","article-title":"Zika virus infection with prolonged maternal viremia and fetal brain abnormalities","author":"Driggers","year":"2016","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.cell.2016.04.032_bib9","doi-asserted-by":"crossref","DOI":"10.1126\/science.aaf5036","article-title":"Zika virus in the Americas: Early epidemiological and genetic findings","author":"Faria","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.cell.2016.04.032_bib10","doi-asserted-by":"crossref","DOI":"10.1126\/science.aaf6116","article-title":"Zika virus impairs growth in human neurospheres and brain organoids","author":"Garcez","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.cell.2016.04.032_bib11","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3181\/00379727-202-43525","article-title":"Rotating-wall vessel coculture of small intestine as a prelude to tissue modeling: aspects of simulated microgravity","volume":"202","author":"Goodwin","year":"1993","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"10.1016\/j.cell.2016.04.032_bib12","doi-asserted-by":"crossref","first-page":"e1477","DOI":"10.1371\/journal.pntd.0001477","article-title":"Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage","volume":"6","author":"Haddow","year":"2012","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.cell.2016.04.032_bib13","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1038\/nature08845","article-title":"Neurogenic radial glia in the outer subventricular zone of human neocortex","volume":"464","author":"Hansen","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.cell.2016.04.032_bib14","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/S0140-6736(16)00320-2","article-title":"Zika virus and microcephaly: why is this situation a PHEIC?","volume":"387","author":"Heymann","year":"2016","journal-title":"Lancet"},{"key":"10.1016\/j.cell.2016.04.032_bib15","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/nn.3898","article-title":"Developmental regulation of human cortex transcription and its clinical relevance at single base resolution","volume":"18","author":"Jaffe","year":"2015","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.cell.2016.04.032_bib16","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1002\/jcb.240510308","article-title":"Prospects for use of microgravity-based bioreactors to study three-dimensional host-tumor interactions in human neoplasia","volume":"51","author":"Jessup","year":"1993","journal-title":"J.\u00a0Cell. Biochem."},{"key":"10.1016\/j.cell.2016.04.032_bib17","doi-asserted-by":"crossref","first-page":"20284","DOI":"10.1073\/pnas.1315710110","article-title":"Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex","volume":"110","author":"Kadoshima","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cell.2016.04.032_bib18","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1016\/j.cell.2011.12.037","article-title":"Interplay between DISC1 and GABA signaling regulates neurogenesis in mice and risk for schizophrenia","volume":"148","author":"Kim","year":"2012","journal-title":"Cell"},{"key":"10.1016\/j.cell.2016.04.032_bib19","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1038\/nature10648","article-title":"Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson\u2019s disease","volume":"480","author":"Kriks","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.cell.2016.04.032_bib20","doi-asserted-by":"crossref","first-page":"9956","DOI":"10.1073\/pnas.1214056110","article-title":"Sex-specific epigenetic disruption and behavioral changes following low-dose in utero bisphenol A exposure","volume":"110","author":"Kundakovic","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cell.2016.04.032_bib21","doi-asserted-by":"crossref","first-page":"1247125","DOI":"10.1126\/science.1247125","article-title":"Organogenesis in a dish: modeling development and disease using organoid technologies","volume":"345","author":"Lancaster","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.cell.2016.04.032_bib22","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/nature12517","article-title":"Cerebral organoids model human brain development and microcephaly","volume":"501","author":"Lancaster","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.cell.2016.04.032_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.chom.2016.03.010","article-title":"A mouse model of Zika virus pathogenesis","author":"Lazear","year":"2016","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.cell.2016.04.032_bib24","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.cell.2011.06.030","article-title":"Development and evolution of the human neocortex","volume":"146","author":"Lui","year":"2011","journal-title":"Cell"},{"key":"10.1016\/j.cell.2016.04.032_bib25","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.cell.2015.06.034","article-title":"FOXG1-dependent dysregulation of GABA\/glutamate neuron differentiation in autism spectrum disorders","volume":"162","author":"Mariani","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.cell.2016.04.032_bib26","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1056\/NEJMoa1600651","article-title":"Zika virus associated with microcephaly","volume":"374","author":"Mlakar","year":"2016","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.cell.2016.04.032_bib27","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.celrep.2014.12.051","article-title":"Self-organization of polarized cerebellar tissue in 3D culture of human pluripotent stem cells","volume":"10","author":"Muguruma","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.cell.2016.04.032_bib28","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.3415","article-title":"Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture","volume":"12","author":"Pa\u015fca","year":"2015","journal-title":"Nat. Methods"},{"key":"10.1016\/j.cell.2016.04.032_bib29","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1093\/cercor\/bhn078","article-title":"Origins of cortical GABAergic neurons in the cynomolgus monkey","volume":"19","author":"Petanjek","year":"2009","journal-title":"Cereb. Cortex"},{"key":"10.1016\/j.cell.2016.04.032_bib30","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.cell.2015.09.004","article-title":"Molecular identity of human outer radial glia during cortical development","volume":"163","author":"Pollen","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.cell.2016.04.032_bib31","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1016\/j.stemcr.2015.05.002","article-title":"KeyGenes, a tool to probe tissue differentiation using a human fetal transcriptional atlas","volume":"4","author":"Roost","year":"2015","journal-title":"Stem Cell Reports"},{"key":"10.1016\/j.cell.2016.04.032_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.stem.2016.02.016","article-title":"Zika virus infects human cortical neural progenitors and attenuates their growth","author":"Tang","year":"2016","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.cell.2016.04.032_bib33","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/nmeth.3629","article-title":"Fixed single-cell transcriptomic characterization of human radial glial diversity","volume":"13","author":"Thomsen","year":"2015","journal-title":"Nat. Methods"},{"key":"10.1016\/j.cell.2016.04.032_bib34","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1006\/dbio.2000.9902","article-title":"The murine Otp homeobox gene plays an essential role in the specification of neuronal cell lineages in the developing hypothalamus","volume":"227","author":"Wang","year":"2000","journal-title":"Dev. Biol."},{"key":"10.1016\/j.cell.2016.04.032_bib35","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1038\/nature13716","article-title":"Synaptic dysregulation in a human iPS cell model of mental disorders","volume":"515","author":"Wen","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.cell.2016.04.032_bib36","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.stem.2015.12.005","article-title":"Engineering Stem Cell Organoids","volume":"18","author":"Yin","year":"2016","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.cell.2016.04.032_bib37","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.stem.2014.05.003","article-title":"Modeling a genetic risk\u00a0for\u00a0schizophrenia in iPSCs and mice reveals neural stem cell deficits associated with adherens junctions and polarity","volume":"15","author":"Yoon","year":"2014","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.cell.2016.04.032_bib38","doi-asserted-by":"crossref","first-page":"2413","DOI":"10.1523\/JNEUROSCI.5249-10.2011","article-title":"Dorsal radial glial cells have the potential to generate cortical interneurons in human but not in mouse brain","volume":"31","author":"Yu","year":"2011","journal-title":"J.\u00a0Neurosci."}],"container-title":["Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0092867416304676?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0092867416304676?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,9,7]],"date-time":"2019-09-07T01:29:09Z","timestamp":1567819749000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0092867416304676"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["S0092867416304676"],"URL":"https:\/\/doi.org\/10.1016\/j.cell.2016.04.032","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.726316695.793560536","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.726316695.793519195","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.726316695.793526393","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.726316695.793528454","asserted-by":"object"}]},"ISSN":["0092-8674"],"issn-type":[{"value":"0092-8674","type":"print"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure","name":"articletitle","label":"Article Title"},{"value":"Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cell.2016.04.032","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Inc.","name":"copyright","label":"Copyright"}]}}