{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T23:48:51Z","timestamp":1792108131294,"version":"4.4.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T00:00:00Z","timestamp":1629417600000},"content-version":"vor","delay-in-days":384,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100007185","name":"University of California, Los Angeles","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007185","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001869","name":"Academia Sinica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001869","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.cell.2020.07.010","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T10:28:04Z","timestamp":1597055284000},"page":"933-946.e14","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":287,"title":["Converting Escherichia coli to a Synthetic Methylotroph Growing Solely on Methanol"],"prefix":"10.1016","volume":"182","author":[{"given":"Frederic Y.-H.","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-Wei","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Yin","family":"Tsuei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cell.2020.07.010_bib1","doi-asserted-by":"crossref","first-page":"2006.0008","DOI":"10.1038\/msb4100050","article-title":"Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection","volume":"2","author":"Baba","year":"2006","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.cell.2020.07.010_bib2","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.ab.2005.06.039","article-title":"A method for the isolation of covalent DNA-protein crosslinks suitable for proteomics analysis","volume":"344","author":"Barker","year":"2005","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.cell.2020.07.010_bib3","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1021\/acssynbio.5b00187","article-title":"Rapid and Efficient One-Step Metabolic Pathway Integration in E.\u00a0coli","volume":"5","author":"Bassalo","year":"2016","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.cell.2020.07.010_bib4","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.3389\/fmicb.2019.01313","article-title":"Continuous Culture Adaptation of Methylobacterium extorquens AM1 and TK 0001 to Very High Methanol Concentrations","volume":"10","author":"Belkhelfa","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.cell.2020.07.010_bib5","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ymben.2017.11.016","article-title":"Expression of heterologous non-oxidative pentose phosphate pathway from Bacillus methanolicus and phosphoglucose isomerase deletion improves methanol assimilation and metabolite production by a synthetic Escherichia coli methylotroph","volume":"45","author":"Bennett","year":"2018","journal-title":"Metab. Eng."},{"key":"10.1016\/j.cell.2020.07.010_bib6","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.copbio.2017.11.010","article-title":"Engineering the bioconversion of methane and methanol to fuels and chemicals in native and synthetic methylotrophs","volume":"50","author":"Bennett","year":"2018","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.cell.2020.07.010_bib7","series-title":"Methanol: the basic chemical and energy feedstock of the future","author":"Bertau","year":"2014"},{"key":"10.1016\/j.cell.2020.07.010_bib8","doi-asserted-by":"crossref","first-page":"15928","DOI":"10.1073\/pnas.1413470111","article-title":"Building carbon-carbon bonds using a biocatalytic methanol condensation cycle","volume":"111","author":"Bogorad","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cell.2020.07.010_bib9","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s00253-006-0757-z","article-title":"Bacillus methanolicus: a candidate for industrial production of amino acids from methanol at 50 degrees C","volume":"74","author":"Brautaset","year":"2007","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.cell.2020.07.010_bib10","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.ymben.2018.08.010","article-title":"Synthetic methanol auxotrophy of Escherichia coli for methanol-dependent growth and production","volume":"49","author":"Chen","year":"2018","journal-title":"Metab. Eng."},{"key":"10.1016\/j.cell.2020.07.010_bib11","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0378-1119(95)00193-A","article-title":"Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant","volume":"158","author":"Cherepanov","year":"1995","journal-title":"Gene"},{"key":"10.1016\/j.cell.2020.07.010_bib12","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1146\/annurev.micro.091208.073600","article-title":"The expanding world of methylotrophic metabolism","volume":"63","author":"Chistoserdova","year":"2009","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.cell.2020.07.010_bib13","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1126\/science.1246929","article-title":"Chemistry. Envisioning the bioconversion of methane to liquid fuels","volume":"343","author":"Conrado","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.cell.2020.07.010_bib14","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.copbio.2019.10.002","article-title":"Renewable methanol and formate as microbial feedstocks","volume":"62","author":"Cotton","year":"2020","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.cell.2020.07.010_bib15","doi-asserted-by":"crossref","first-page":"e02038-17","DOI":"10.1128\/AEM.02038-17","article-title":"Genetic Plasticity and Ethylmalonyl Coenzyme A Pathway during Acetate Assimilation in Rhodospirillum rubrum S1H under Photoheterotrophic Conditions","volume":"84","author":"De Meur","year":"2018","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.cell.2020.07.010_bib16","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1038\/s41587-019-0363-0","article-title":"The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2","volume":"38","author":"Gassler","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cell.2020.07.010_bib17","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.cell.2019.11.009","article-title":"Conversion of Escherichia coli to Generate All Biomass Carbon from CO2","volume":"179","author":"Gleizer","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.cell.2020.07.010_bib18","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.ymben.2017.11.015","article-title":"Methanol assimilation in Escherichia coli is improved by co-utilization of threonine and deletion of leucine-responsive regulatory protein","volume":"45","author":"Gonzalez","year":"2018","journal-title":"Metab. Eng."},{"key":"10.1016\/j.cell.2020.07.010_bib19","doi-asserted-by":"crossref","first-page":"2506","DOI":"10.1128\/AEM.04023-14","article-title":"Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system","volume":"81","author":"Jiang","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.cell.2020.07.010_bib20","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1038\/s41589-020-0473-5","article-title":"Growth of E.\u00a0coli on formate and methanol via the reductive glycine pathway","volume":"16","author":"Kim","year":"2020","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.cell.2020.07.010_bib21","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0304-4165(75)90232-9","article-title":"Regulation of the amount and of the activity of phosphofructokinases and pyruvate kinases in Escherichia coli","volume":"381","author":"Kotlarz","year":"1975","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.cell.2020.07.010_bib22","doi-asserted-by":"crossref","first-page":"3827","DOI":"10.1002\/anie.201611379","article-title":"Photoelectrochemical Reduction of Carbon Dioxide to Methanol through a Highly Efficient Enzyme Cascade","volume":"56","author":"Kuk","year":"2017","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.cell.2020.07.010_bib23","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ymben.2014.06.006","article-title":"Ensemble Modeling for Robustness Analysis in engineering non-native metabolic pathways","volume":"25","author":"Lee","year":"2014","journal-title":"Metab. Eng."},{"key":"10.1016\/j.cell.2020.07.010_bib24","doi-asserted-by":"crossref","first-page":"3538","DOI":"10.1073\/pnas.1802191115","article-title":"Construction and evolution of an Escherichia coli strain relying on nonoxidative glycolysis for sugar catabolism","volume":"115","author":"Lin","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cell.2020.07.010_bib25","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1038\/s41467-018-03937-y","article-title":"Methanol-essential growth of Escherichia coli","volume":"9","author":"Meyer","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.cell.2020.07.010_bib26","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s12934-017-0661-5","article-title":"Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris","volume":"16","author":"Moser","year":"2017","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.cell.2020.07.010_bib27","doi-asserted-by":"crossref","first-page":"e107887","DOI":"10.1371\/journal.pone.0107887","article-title":"Genetic and phenotypic comparison of facultative methylotrophy between Methylobacterium extorquens strains PA1 and AM1","volume":"9","author":"Nayak","year":"2014","journal-title":"PLoS ONE"},{"key":"10.1016\/j.cell.2020.07.010_bib28","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1186\/s40064-015-1415-7","article-title":"Mechanism to control the cell lysis and the cell survival strategy in stationary phase under heat stress","volume":"4","author":"Noor","year":"2015","journal-title":"Springerplus"},{"key":"10.1016\/j.cell.2020.07.010_bib29","doi-asserted-by":"crossref","first-page":"18968","DOI":"10.1021\/acsami.9b03420","article-title":"Hierarchical Macroporous Particles for Efficient Whole-Cell Immobilization: Application in Bioconversion of Greenhouse Gases to Methanol","volume":"11","author":"Patel","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.cell.2020.07.010_bib30","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1021\/pr8009319","article-title":"Exploring DNA-binding proteins with in\u00a0vivo chemical cross-linking and mass spectrometry","volume":"8","author":"Qin","year":"2009","journal-title":"J.\u00a0Proteome Res."},{"key":"10.1016\/j.cell.2020.07.010_bib31","first-page":"1","article-title":"An entropy-like index of bifurcational robustness for metabolic systems","volume":"7","author":"Rivera","year":"2015","journal-title":"Integr. Biol. (Camb.)"},{"key":"10.1016\/j.cell.2020.07.010_bib32","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/S0022-5320(78)90049-7","article-title":"A technique for observing extended DNA in negatively stained specimens: observation of bacteriophage T7 capsid-DNA complexes","volume":"65","author":"Serwer","year":"1978","journal-title":"J.\u00a0Ultrastruct. Res."},{"key":"10.1016\/j.cell.2020.07.010_bib33","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/gm62","article-title":"Copy number variations and cancer","volume":"1","author":"Shlien","year":"2009","journal-title":"Genome Med."},{"key":"10.1016\/j.cell.2020.07.010_bib34","doi-asserted-by":"crossref","first-page":"e13001","DOI":"10.1371\/journal.pone.0013001","article-title":"Methanol assimilation in Methylobacterium extorquens AM1: demonstration of all enzymes and their regulation","volume":"5","author":"Smejkalov\u00e1","year":"2010","journal-title":"PLoS ONE"},{"key":"10.1016\/j.cell.2020.07.010_bib35","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1038\/nrm4015","article-title":"DNA-protein crosslink repair","volume":"16","author":"Stingele","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.cell.2020.07.010_bib36","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.ymben.2018.07.011","article-title":"Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production","volume":"49","author":"Tuyishime","year":"2018","journal-title":"Metab. Eng."},{"key":"10.1016\/j.cell.2020.07.010_bib37","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1038\/nbt.1555","article-title":"Stabilized gene duplication enables long-term selection-free heterologous pathway expression","volume":"27","author":"Tyo","year":"2009","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.cell.2020.07.010_bib38","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1038\/s41467-018-04795-4","article-title":"Improving formaldehyde consumption drives methanol assimilation in engineered E.\u00a0coli","volume":"9","author":"Woolston","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.cell.2020.07.010_bib39","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.ab.2012.01.012","article-title":"Glyceraldehyde-3-phosphate dehydrogenase: a universal internal control for Western blots in prokaryotic and eukaryotic cells","volume":"423","author":"Wu","year":"2012","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.cell.2020.07.010_bib40","doi-asserted-by":"crossref","first-page":"4969","DOI":"10.1007\/s00253-016-7320-3","article-title":"Characterization and evolution of an activator-independent methanol dehydrogenase from Cupriavidus necator N-1","volume":"100","author":"Wu","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.cell.2020.07.010_bib41","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1093\/gbe\/evr023","article-title":"Copy-number variation: the balance between gene dosage and expression in Drosophila melanogaster","volume":"3","author":"Zhou","year":"2011","journal-title":"Genome Biol. Evol."}],"container-title":["Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0092867420308758?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0092867420308758?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T11:46:50Z","timestamp":1758800810000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0092867420308758"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["S0092867420308758"],"URL":"https:\/\/doi.org\/10.1016\/j.cell.2020.07.010","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.738464837.793577999","asserted-by":"object"}]},"ISSN":["0092-8674"],"issn-type":[{"value":"0092-8674","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Converting Escherichia coli to a Synthetic Methylotroph Growing Solely on Methanol","name":"articletitle","label":"Article Title"},{"value":"Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cell.2020.07.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc.","name":"copyright","label":"Copyright"}]}}