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Carbachol added basolaterally induced a concentration\u2010dependent increase in short\u2010circuit current (Isc) across the epididymal epithelium consisting of a rapidly rising phase and a long term sustained response. The response was almost abolished by removing Cl<jats:sup>\u2212<\/jats:sup> from the extracellular medium and blockable by pretreating the tissues with DPC, indicating a substantial contribution of Cl<jats:sup>\u2212<\/jats:sup> secretion to the carbachol\u2010induced response. The muscarinic acetylcholine receptor antagonist atropine inhibited the response, but the nicotinic acetylcholine receptors antagonist curarine had no effect, suggesting that only the muscarinic acetylcholine receptors mediated the secretory response of the basolateral side of rat cauda epididymal epithelium to carbachol. Addition of carbachol to the apical side of the tissue was found not to elicit an Isc response. These results suggested that muscarinic receptors are present in the basolateral side of rat cauda epididymal epithelium. Activation of these receptors by acetylcholine released from the nerve endings regulates epididymal transepithelial Cl<jats:sup>\u2212<\/jats:sup> secretion. Cholinergic stimulation therefore contributes to the formation of luminal fluid microenvironment.<\/jats:p>","DOI":"10.1016\/j.cellbi.2006.05.008","type":"journal-article","created":{"date-parts":[[2006,5,23]],"date-time":"2006-05-23T20:50:00Z","timestamp":1148417400000},"page":"741-746","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Involvement of muscarinic acetylcholine receptors in chloride secretion by cultured rat epididymal epithelium"],"prefix":"10.1002","volume":"30","author":[{"given":"Jian\u2010Yang","family":"Du","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wu\u2010Lin","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min\u2010Hui","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Liang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233281"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.4.C958"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.26.3.f232"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041640207"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233546"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016222"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0922-4106(92)90102-2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.48.030186.001031"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1992.tb09436.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001210102"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/15.10.580"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019311"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"C794","DOI":"10.1152\/ajpcell.1993.264.4.C794","article-title":"Properties of cAMP\u2010dependent and Ca(2+)\u2010dependent whole cell Cl\u2212 conductances in rat epididymal cells","volume":"264","author":"Huang S.J.","year":"1993","journal-title":"Am J Physiol"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020012"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod23.5.981"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.262.1.C15"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13599.x"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019384"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00388302"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1992.tb14477.x"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.4.1120"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(97)00045-3"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(95)00036-6"},{"key":"e_1_2_1_25_1","first-page":"197","article-title":"The autonomic nervous system","volume":"8","author":"Richins C.A.","year":"1953","journal-title":"Prog Neurol Psychiatry"},{"key":"e_1_2_1_26_1","first-page":"1035","article-title":"Quantification of steady state expression of mRNA for alpha\u20101 adrenergic receptor subtypes using reverse transcription and a competitive polymerase chain reaction","volume":"275","author":"Scofield M.A.","year":"1995","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.79.1.86"},{"key":"e_1_2_1_28_1","first-page":"499","article-title":"Microscopic innervation of the spermatic ducts and testis. 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