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MMPs have been found in rat prostate secretions and are nearly lobe specific in expression pattern. The aim of this study was to evaluate whether TIMP\u20102, like other semen components, is expressed differently from different rat prostatic lobes. Immunohistochemical staining was performed in both young and adult rat ventral (VP), lateral (LP), dorsal (DP), and anterior (AP) prostatic lobes and confirmed by western blotting. TIMP\u20102 expression was found in the epithelial cells in the following sequence: LP &gt; AP &gt; DP &gt; VP, in both young and adult rats. In this study, 100% of adult LP presented histological signs of prostatitis, where TIMP\u20102 immunostaining was positive in normal epithelium even with intraluminal neutrophils, but was reduced or absent in the epithelium with intraepithelial leukocytes or with periductal stroma disorganization associated with mononuclear cell infiltration. However, TIMP\u20102 expression in LP was not induced by prostatitis, since younger rat LPs were also strongly TIMP\u20102 positive. The distal and intermediate VP regions were TIMP\u20102 negative, but the proximal regions were strongly stained. Western blotting results confirmed the high TIMP\u20102 expression in the LP lobe. Thus, TIMP\u20102 is expressed differently between the prostatic lobes and is another nearly lobe\u2010specific protein, which plays a role in the regulation of MMP activity in seminal plasma and glandular homeostasis. TIMP\u20102 is also another regional ductal variation of VP. Further studies should address whether TIMP\u20102 expression is related to the highest incidence of rat LP prostatitis and adenocarcinoma.<\/jats:p>","DOI":"10.1016\/j.cellbi.2006.10.004","type":"journal-article","created":{"date-parts":[[2006,10,19]],"date-time":"2006-10-19T13:23:10Z","timestamp":1161264190000},"page":"229-234","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Tissue inhibitor of metalloproteinase\u20102 (TIMP\u20102) location in the ventral, lateral, dorsal and anterior lobes of rat prostate by immunohistochemistry"],"prefix":"10.1002","volume":"31","author":[{"given":"Fl\u00e1via K.","family":"Delella","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Luis A.","family":"Justulin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9rgio 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