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Cryopreserved hMSCs were resuscitated and cultured for 15 passages, and then induced to adipocytes and neurocytes with corresponding induction medium. The induced cells were observed for morphological properties and expression of triglyceride or neuron\u2010specific enolase and nestin was detected. The result showed that the resuscitated cells cultured in induction medium consisting of dexamethasone, 3\u2010isobutyl\u20101\u2010methylxanthine, indomethacin and insulin\u2010like growth factor I (IGF\u2010I) showed adipogenesis, and lipid vacuole accumulation was detectable after 21 days. The resuscitated hMSCs were also induced into neurocytes and expressed nestin and neuron\u2010specific enolase (NSE), which are special surface markers associated with neural cells at different stages. 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