{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T10:10:01Z","timestamp":1699956601784},"reference-count":11,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":2073,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Our previous study demonstrated that NYD\u2010SP27 is a novel inhibitory PLC isoform expressed endogenously in human pancreas and upregulated in CFPAC\u20101 cells. The present study investigated the effect of NYD\u2010SP27 down\u2010regulation on the ATP\u2010stimulated and Ca<jats:sup>2+<\/jats:sup>\u2010dpendent pancreatic anion secretion by CFPAC\u20101 cell line using short\u2010circuit current (<jats:italic>I<\/jats:italic> <jats:sub>SC<\/jats:sub>) recording. NYD\u2010SP27 antisense\u2010transfected CFPAC\u20101 (AT\u2010CF) cells exhibited a significantly higher basal transmembrane potential difference and current than those of empty vector\u2010transfected CFPAC\u20101 (VT\u2010CF) cells. Cl<jats:sup>\u2212<\/jats:sup> channel blocker, DPC or Glibenclamide (1 mM), and inhibitor of Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2010Cl<jats:sup>\u2212<\/jats:sup> cotransporter, bumetanide (100 \u03bcM) significantly inhibited the basal current in AT\u2010CF cells. The inhibitor of adenylate cyclase, MDL12330A (20 \u03bcM), and Ca<jats:sup>2+<\/jats:sup>\u2010dependent Cl<jats:sup>\u2212<\/jats:sup> channel (CaCC) blocker, DIDS (100 \u03bcM) also significantly reduced the basal current in AT\u2010CF. Apical application of ATP (10 \u03bcM) stimulated a fast transient <jats:italic>I<\/jats:italic> <jats:sub>SC<\/jats:sub> increase in VT\u2010CF cells, but a more sustained rise with slower decline in AT\u2010CF cells. Pretreatment with BAPTA\u2010AM (50 \u03bcM) reduced the ATP\u2010induced <jats:italic>I<\/jats:italic> <jats:sub>SC<\/jats:sub> response in AT\u2010CF cells by 77.9%. PMA (1 \u03bcM), a PKC activator, inhibited the ATP\u2010stimulated current increase (the transient peak) in VT\u2010CF cells, but had no effect on the AT\u2010CF cells. However, PKC inhibitor, staurosporine (40 \u03bcM) could inhibit the ATP\u2010induced <jats:italic>I<\/jats:italic> <jats:sub>SC<\/jats:sub> response in AT\u2010CF cells. The present results confirm the previously proposed inhibitory role of NYD\u2010SP27 in the PLC pathway and demonstrate that the suppression of its expression could result in an enhancement of ATP\u2010stimulated Ca<jats:sup>2+<\/jats:sup> dependent pancreatic anion secretion.<\/jats:p>","DOI":"10.1016\/j.cellbi.2006.11.021","type":"journal-article","created":{"date-parts":[[2006,11,29]],"date-time":"2006-11-29T22:25:15Z","timestamp":1164839115000},"page":"521-525","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of NYD\u2010SP27 down\u2010regulation on ATP\u2010induced Ca<sup>2+<\/sup>\u2010dependent pancreatic duct anion secretion in cystic fibrosis cells"],"prefix":"10.1002","volume":"31","author":[{"given":"Jin Xia","family":"Zhu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hu","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiu Wa","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsiao Chang","family":"Chan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90072-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.056697"},{"issue":"2","key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"C469","DOI":"10.1152\/ajpcell.1996.271.2.C469","article-title":"Purinergic regulation of anion secretion by cystic fibrosis pancreatic duct cells","volume":"271","author":"Chan H.C.","year":"1996","journal-title":"Am J Physiol"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90446-6"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(99)00011-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.57.030195.002131"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.5.2872"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2475911"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/352628a0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90353-R"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15100-8"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.cellbi.2006.11.021","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.cellbi.2006.11.021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T09:56:21Z","timestamp":1699955781000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1016\/j.cellbi.2006.11.021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5]]},"references-count":11,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,5]]}},"alternative-id":["10.1016\/j.cellbi.2006.11.021"],"URL":"https:\/\/doi.org\/10.1016\/j.cellbi.2006.11.021","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}