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Western blot analysis showed that the expression of flotillin\u20101 increased significantly 6 h after I\/R and that the level remained elevated for 48 h. Immunohistochemically, flotillin\u20101 was constitutively localized in some Sertoli cells, peritubular myoid cells, and interstitial cells in the normal testis. After I\/R injury, Sertoli cells in the damaged tubules were intensely immunostained for flotillin\u20101 at 24 and 48 h after I\/R. Flotillin\u20101 was also detected in some inflammatory cells in the interstitial space around damaged tubules. Furthermore, flotillin\u20101 was colocalized with cathepsin D, a lysosomal marker, in normal testis (mainly in Sertoli cells), and the colocalization was greater in Sertoli cells and macrophages in I\/R injured testes. Therefore, we postulate that flotillin\u20101 immunoreactivity is increased in some Sertoli and inflammatory cells (especially in ED1\u2010positive activated macrophages) in testicular torsion and that flotillin\u20101 in the injured testis associates with lysosomes in Sertoli cells and macrophages, activating subsequent signals in inflammatory macrophages and Sertoli cells after I\/R.<\/jats:p>","DOI":"10.1016\/j.cellbi.2006.11.035","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T12:13:37Z","timestamp":1165407217000},"page":"609-614","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Immunohistochemical study of flotillin\u20101 in rat testis with ischemia\/reperfusion 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