{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T15:34:34Z","timestamp":1773675274807,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":2103,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inflammation, malnutrition and growth retardation during critical time\u2010windows of development play a powerful role in ontogenetic programming of the life\u2010long risk to many adult diseases (including metabolic syndrome, obesity and diabetes). Cellular mechanisms and the accurate timing and duration of critical periods for the liver remain obscure. To resolve this problem, we developed a postnatal suckling\u2010weanling rat model of mild, moderate, and acute gastroenteritis challenged by a protozoan parasitic spread throughout the whole world, namely <jats:italic>Cryptosporidium parvum<\/jats:italic>. The physiological state of the liver was evaluated by hepatocyte ploidy and protein content that were measured by cytophotometry and image analysis on isolated cells. Hepatocyte ploidy is known to irreversibly increase after stress and is associated with the decrease in liver physiological capacity. Hepatocyte hypertrophy reflects cell functional loading. From our results, cryptosporidiosis is able to provoke a burst in premature hepatocyte polyploidization and hypertrophy (in proportion to parasitic load), and thus plays an important role in epigenetic programming of hepatocyte structure and function. We revealed two sensitive periods in liver growth. The first period (the less sensitive) covers the time before the establishment of homoiothermy, i.e. 6\u20139 days after birth. The second period (the more sensitive) covers the time of weaning when the change of type of nutrition and the peak of hepatocyte polyploidization and differentiation occurs. Thus, our data provide direct evidence that phenomenon of ontogenetic programming is reflected at the cellular level.<\/jats:p>","DOI":"10.1016\/j.cellbi.2007.01.028","type":"journal-article","created":{"date-parts":[[2007,1,22]],"date-time":"2007-01-22T14:18:57Z","timestamp":1169475537000},"page":"420-427","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Impact of neonatal cryptosporidial gastroenteritis on epigenetic programming of rat hepatocytes"],"prefix":"10.1002","volume":"31","author":[{"given":"Olga V.","family":"Anatskaya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina V.","family":"Sidorenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander E.","family":"Vinogradov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara V.","family":"Beyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/rtph.1998.1201"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8312.2004.00377.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2006.08.014"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.2307\/3282084"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.56.012302.160616"},{"key":"e_1_2_1_7_1","first-page":"1046","article-title":"Morphofunctional changes in hepatocytes during the early postnatal development of rats experimentally infected with the intestinal protozoan pathogen, Cryptosporidium parvum (Coccidia, Sporozoa)","volume":"44","author":"Beier T.V.","year":"2002","journal-title":"Tsitologiia"},{"key":"e_1_2_1_8_1","first-page":"3","article-title":"Intestinal cryptosporidiosis at an early age and its negative consequences","volume":"1","author":"Beier T.V.","year":"2006","journal-title":"Med Parazitol (Mosk)"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60100-X"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840380422"},{"key":"e_1_2_1_11_1","first-page":"17","article-title":"A cytophotometric method for estimation of histones and non\u2010histones proteins","volume":"13","author":"Deltch A.L.","year":"1965","journal-title":"J Histochem Cytochem"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.1997.tb00016.x"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.09.004"},{"key":"e_1_2_1_14_1","first-page":"1","volume-title":"Cryptosporidium and Cryptosporidiosis","author":"Fayer R.","year":"1997"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00135-1"},{"key":"e_1_2_1_16_1","unstructured":"FilimonovNYu.Principles of classification with regard to the parasite's influence on the host. (abstract). Proc 29th Interdepartmental Scientific\u2010Practical Confer Problems Biol Med Parasitol2002;11\u201314."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092556"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5425.251"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300982200"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.2000.0317"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1054\/mehy.2000.1111"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.spid.2004.07.006"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00740"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540305100307"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208601200"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.00108s3545"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.3.635"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510280435"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(97)80358-3"},{"key":"e_1_2_1_30_1","first-page":"48","article-title":"Methods of cryptosporidian diagnosis (Oocysts microscopic detection)","volume":"2","author":"Sidorenko N.V.","year":"1988","journal-title":"Veterinary"},{"key":"e_1_2_1_31_1","first-page":"114","article-title":"Cell response of rat liver parenchyma to the infection by the intestinal protozoan pathogen Cryptosporidium parvum (Coccidia, Sporozoa)","volume":"46","author":"Sidorenko N.V.","year":"2004","journal-title":"Tsitologiia"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-0436.1995.5910035.x"},{"key":"e_1_2_1_33_1","volume-title":"Electronic Statistics Textbook","author":"StatSoft Inc","year":"2002"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19980201)31:2<100::AID-CYTO5>3.0.CO;2-Q"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1139\/g01-015"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(72)90401-6"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/01926230252824699"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.cellbi.2007.01.028","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.cellbi.2007.01.028","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T14:02:57Z","timestamp":1700056977000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1016\/j.cellbi.2007.01.028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1016\/j.cellbi.2007.01.028"],"URL":"https:\/\/doi.org\/10.1016\/j.cellbi.2007.01.028","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}