{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T06:55:12Z","timestamp":1700031312402},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":1950,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adherence of <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> to human gingival fibroblast cells induces cytoskeletal reorganization. <jats:italic>A. actinomycetemcomitans<\/jats:italic> is considered a pathogenic bacteria involved in localized aggressive periodontitis. Studies with epithelial cells have shown an adherent capacity of bacteria that is increased under anaerobic conditions. For adherence to take place, there is a need for interaction between extracellular vesicles and bacterial fimbriae. However, molecular events associated with the adherence process are still unknown. The aim of this study was to\u00a0investigate whether <jats:italic>A. actinomycetemcomitans<\/jats:italic> adherence to human gingival fibroblasts promotes cytoskeletal reorganization. Adherence was\u00a0determined with light microscopy and scanning electron microscopy. For F\u2010actin visualization, cells were treated with fluorescein\u2014isothiocyanate\u2014phalloidin and samples were examined with epifluorescence optics. Fluorescent was recorded on Kodak T\u2010Max 400 film.<\/jats:p><jats:p>We showed that <jats:italic>A. actinomycetemcomitans<\/jats:italic> adheres to human gingival fibroblast primary cultures, this property stimulating an increase in the intracellular calcium levels. In human gingival fibroblast primary cultures, we observed that maximal <jats:italic>A. actinomycetemcomitans<\/jats:italic> adherence took place 1.5 h after culture infection occurred and remained for 6 h. The adherence was associated with morphologic alterations and an increased in the intracellular calcium levels. These experiments suggest that <jats:italic>A. actinomycetemcomitans<\/jats:italic> adherence cause morphological alterations, induce actin stress fibers and recruitment of intracellular calcium levels.<\/jats:p>","DOI":"10.1016\/j.cellbi.2007.03.028","type":"journal-article","created":{"date-parts":[[2007,3,28]],"date-time":"2007-03-28T11:19:14Z","timestamp":1175080754000},"page":"1063-1068","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>Actinobacillus actinomycetemcomitans<\/i> adheres to human gingival fibroblasts and modifies cytoskeletal organization"],"prefix":"10.1002","volume":"31","author":[{"given":"Gloria","family":"Guti\u00e9rrez\u2010Venegas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perla","family":"Kawasaki\u2010C\u00e1rdenas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla Portillo","family":"Garc\u00e9s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Rom\u00e1n\u2010Alv\u00e1rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Barajas\u2010Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Arturo","family":"Contreras\u2010Marmolejo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/000992288102001007"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1128\/iai.59.5.1599-1604.1991","article-title":"Elevation of intracellular free calcium levels in HEp\u20102 cells infected with enteropathogenic Escherichia coli","volume":"59","author":"Baldwin T.J.","year":"1991","journal-title":"Infect Immun"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1128\/iai.61.2.760-763.1993","article-title":"Calcium calmodulin dependence of actin accretion and lethality in cultured HEp\u20102 cells infected with enteropathogenic Escherichia coli","volume":"61","author":"Baldwin T.J.","year":"1993","journal-title":"Infect Immun"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.2.980"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-197311000-00006"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02602439"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(99)80062-8"},{"issue":"2","key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1242\/jcs.99.2.283","article-title":"Cytoskeletal rearrangements accompanying salmonella entry into epithelial cells","volume":"99","author":"Finlay B.B.","year":"1991","journal-title":"J Cell Sci"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1996.67.3s.291"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.41.3.1190-1196.1983"},{"key":"e_1_2_1_12_1","first-page":"297","article-title":"Isolation and characterization of fimbrial proteins from Actinobacillus actinomycetemcomitans","volume":"69","author":"Gillespie M.J.","year":"1990","journal-title":"J Dent Res"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.57.030502.090908"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1991.tb00483.x"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1990.tb04167.x"},{"key":"e_1_2_1_17_1","first-page":"654","article-title":"Actinobacillus actinomycetemcomitans strains Y4 and N27 adhere to hydroxyapatite by distinctive mechanisms infect","volume":"47","author":"Kagermieier A.S.","year":"1985","journal-title":"Immun"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.55.1.78-85.1987"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.2.588"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.17.9.833"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1128\/iai.62.3.928-935.1994","article-title":"Characteristics of adherence of A. actinomycetemcomitans to epithelial cells","volume":"62","author":"Meyer D.H.","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.8.2988-2997.1996"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1128\/iai.59.8.2719-2726.1991","article-title":"Evidence for invasion of a human oral cell line by Actinobacillus actinomycetemcomitans","volume":"59","author":"Meyer D.H.","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302X.1999.140206.x"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6580-6586.2000"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1988.tb00082.x"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05438.x"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1992.63.9.723"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345880670011301"},{"key":"e_1_2_1_30_1","first-page":"4036","article-title":"Requirements for invasion of epithelial cells by Actinobacillus actinomycetemcomitans","volume":"58","author":"Sreenivasan P.K.","year":"1993","journal-title":"Infect Immun"},{"issue":"6","key":"e_1_2_1_31_1","doi-asserted-by":"crossref","first-page":"2450","DOI":"10.1128\/iai.62.6.2450-2458.1994","article-title":"Adherence of group B streptococci to cultured epithelial cells: roles of environmental factors and bacterial surface components","volume":"62","author":"Tamura G.S.","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_1_32_1","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1001\/jama.1969.03160290110041","article-title":"Urinary tract infection due to Actinobacillus actinomycetemcomitans","volume":"210","author":"Towsend T.R.","year":"1969","journal-title":"J Am Med Assoc"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1985.tb01348.x"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.cellbi.2007.03.028","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.cellbi.2007.03.028","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T06:23:54Z","timestamp":1700029434000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1016\/j.cellbi.2007.03.028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":32,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1016\/j.cellbi.2007.03.028"],"URL":"https:\/\/doi.org\/10.1016\/j.cellbi.2007.03.028","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}