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In this study, mRNA expressions of hard tissue\u2010related proteins, calcification and proliferation are examined in cultures of human pulp (HP) cells. Nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin (NT)\u20103 and NT\u20104\/5 increased the mRNA levels of dentin sialophsphoprotein, alkaline phosphatase, osteopontin, type I collagen and bone morphogenetic protein\u20102 and mineral deposition in cultures of HP cells. The increased levels and manners varied, depending on the concentrations of NTs and hard\u2010tissue related protein tested. On the other hand, only NGF significantly stimulated DNA synthesis in cultures of HP cells. These findings suggest that NTs characteristically regulate hard\u2010tissue related protein expression, calcification and proliferation in pulp cells. NTs may accelerate pulp cell differentiation.<\/jats:p>","DOI":"10.1016\/j.cellbi.2007.06.012","type":"journal-article","created":{"date-parts":[[2007,7,18]],"date-time":"2007-07-18T11:45:25Z","timestamp":1184759125000},"page":"1462-1469","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Effect of neurotrophins on differentiation, calcification and proliferation in cultures of human pulp 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