{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T15:59:52Z","timestamp":1779119992928,"version":"3.51.4"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":1828,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An effect of magnesium on calcium\u2010induced depolarisation of mitochondrial transmembrane potential (\u0394\u03a8<jats:sub>m<\/jats:sub>) was investigated. Depending on the presence of Mg<jats:sup>2+<\/jats:sup>, addition of Ca<jats:sup>2+<\/jats:sup> to suspension of isolated rat heart mitochondria induced either reversible depolarisation or irreversible collapse of succinate\u2010driven \u0394\u03a8<jats:sub>m<\/jats:sub>. Irreversible collapse of \u0394\u03a8<jats:sub>m<\/jats:sub>, observed in the absence of Mg<jats:sup>2+<\/jats:sup>, was insensitive to Ca<jats:sup>2+<\/jats:sup> chelation, inhibition of Ca<jats:sup>2+<\/jats:sup> uptake and increased efflux of Ca<jats:sup>2+<\/jats:sup> from mitochondrial matrix. Based on these data, opening of mPTP in a high\u2010conductance mode is considered to be a major cause of the Ca<jats:sup>2+<\/jats:sup>\u2010induced irreversible collapse of \u0394\u03a8<jats:sub>m<\/jats:sub> in the absence of Mg<jats:sup>2+<\/jats:sup>. Involvement of mPTP in the process of Ca<jats:sup>2+<\/jats:sup>\u2010induced collapse of \u0394\u03a8<jats:sub>m<\/jats:sub> was further supported by protective effect of both CsA and ADP. Reversible collapse of \u0394\u03a8<jats:sub>m<\/jats:sub>, observed in the presence of Mg<jats:sup>2+<\/jats:sup>, was sensitive to EGTA, ADP; and inhibition of Ca<jats:sup>2+<\/jats:sup> uptake and increased efflux of Ca<jats:sup>2+<\/jats:sup> from mitochondrial matrix. This may represent selective induction of a low\u2010conductance permeability pathway. Presented results indicate important role of Mg<jats:sup>2+<\/jats:sup> in the process of Ca<jats:sup>2+<\/jats:sup>\u2010induced depolarisation of \u0394\u03a8<jats:sub>m<\/jats:sub> mainly through discrimination between low\u2010 and high\u2010conductance modes of mPTP. Minor effect of Mg<jats:sup>2+<\/jats:sup> on Ca<jats:sup>2+<\/jats:sup>\u2010induced depolarisation of \u0394\u03a8<jats:sub>m<\/jats:sub> was observed at the level of stimulation of \u0394\u03a8<jats:sub>m<\/jats:sub> generation and inhibition of mitochondrial Ca<jats:sup>2+<\/jats:sup> uptake.<\/jats:p>","DOI":"10.1016\/j.cellbi.2007.08.024","type":"journal-article","created":{"date-parts":[[2007,9,8]],"date-time":"2007-09-08T11:19:13Z","timestamp":1189250353000},"page":"136-145","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Effect of magnesium on calcium\u2010induced depolarisation of mitochondrial transmembrane 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