{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:19:42Z","timestamp":1777439982549,"version":"3.51.4"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":1554,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Plasminogen activator inhibitor type 1 (PAI\u20101), produced partly from liver is a risk factor for macrovascular and microvascular complications of diabetes. Ghrelin, a recently described orexigenic peptide hormone, attenuates PAI\u20101 induced by TNF\u2010\u03b1 in the human hepatoma cell line (HepG2). Exposure to TNF\u2010\u03b1 (1 ng\/ml) for 24 h caused a significant increase in PAI\u20101 mRNA expression and protein secretion, as evaluated by RT\u2010PCR and ELISA, but pretreatment with ghrelin (1\u2013100 ng\/ml) inhibited both basal and TNF\u2010\u03b1\u2010induced PAI\u20101 release in a dose and time\u2010dependent manner in HepG2. PDTC, selective NF\u2010\u03baB inhibitor, had no additive inhibitory effects with ghrelin. The results indicate that ghrelin inhibits both basal and TNF\u2010\u03b1\u2010induced PAI\u20101 production via NF\u2010\u03baB pathway in HepG2 cells, and suggest that the peptide plays a therapeutic role in atherosclerosis, especially in obese patients with insulin resistance, in whom ghrelin levels were reduced.<\/jats:p>","DOI":"10.1016\/j.cellbi.2008.07.019","type":"journal-article","created":{"date-parts":[[2008,7,28]],"date-time":"2008-07-28T16:50:14Z","timestamp":1217263814000},"page":"1310-1317","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Ghrelin attenuates plasminogen activator inhibitor\u20101 production induced by tumor necrosis factor\u2010\u03b1 in HepG2 cells via NF\u2010\u03baB pathway"],"prefix":"10.1002","volume":"32","author":[{"given":"Liying","family":"Ding","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoliang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenshi","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihong","family":"Zong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000077401.36885.BB"},{"key":"e_1_2_1_3_1","first-page":"532","article-title":"A reagent for the single\u2010step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples","volume":"15","author":"Chomczynski P.","year":"1993","journal-title":"Biotechniques"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200421134"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2005.09.003"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-1069"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.87.6.8739"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.12.3408"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6248960"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/45230"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000127956.43874.F2"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103898200"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002-220756"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2006.06.016"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0899"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.86.12.8111"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.cellbi.2008.07.019","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.cellbi.2008.07.019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T08:14:10Z","timestamp":1700036050000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1016\/j.cellbi.2008.07.019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10]]},"references-count":15,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["10.1016\/j.cellbi.2008.07.019"],"URL":"https:\/\/doi.org\/10.1016\/j.cellbi.2008.07.019","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}