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Erythrocyte suspensions from healthy donors were incubated with increasing concentrations of dithiothreitol (1\u201350 \u03bcM), in the presence and absence of acetylcholine\/velnacrine (10 \u03bcM). Levels of NO, nitrite\/nitrate, S\u2010nitrosohemoglobin, peroxynitrite and S\u2010nitrosoglutathione were determined by spectrofluorimetric and spectrophotometric methods.<\/jats:p><jats:p>Dithiothreitol significantly mobilized NO toward nitrite\/nitrate and S\u2010nitrosoglutathione, and decreased the amount of NO efflux. Both ACh\/VM induce changes on the levels of erythrocyte nitrite\/nitrate dependent on the DTT concentration. Higher levels of peroxynitrite and S\u2010nitrosoglutathione were seen with velnacrine in presence of DTT 1 and 50 \u03bcM.<\/jats:p><jats:p>We concluded that dithiothreitol\u2010induced activation of erythrocyte thiol status decreases NO efflux and allows greater intracellular NO mobilization onto different derivative molecules, both in the absence and presence of acetylcholinesterase substrate and inhibitor.<\/jats:p>","DOI":"10.1016\/j.cellbi.2008.11.012","type":"journal-article","created":{"date-parts":[[2008,12,12]],"date-time":"2008-12-12T14:14:56Z","timestamp":1229091296000},"page":"268-275","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Redox thiol status plays a central role in the mobilization and metabolism of nitric oxide in human red blood cells"],"prefix":"10.1002","volume":"33","author":[{"given":"Jos\u00e9 Pedro","family":"de 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