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Rapamycin is a highly specific inhibitor of mammalian target of rapamycin (mTOR). Rapamycin exerts its biological activity by inhibiting the serine\u2013threonine kinase mTOR, which regulates important cellular processes such as control of cell cycle, cell size, translation initiation and transcription. The ability of rapamycin to cause G1\u2010cell cycle arrest, reduced cell growth and a reduced rate of proliferation has led to efforts to develop rapamycin and related mTOR inhibitors as anti\u2010cystogenesis agents. Therefore, we investigated the relationship between the decreased FPC and the protein levels of mTOR and the inhibitory effects of rapamycin on the expression of mTOR, Hypoxia\u2010inducible factor\u20101 alpha (HIF\u20101\u03b1) and vascular endothelial growth factor (VEGF) in the human 293T cell line. These observations should provide an important platform for determining FPC function and the pathogenesis of ARPKD, with the targeting of mTOR signaling being exploitable as a novel therapy.<\/jats:p>","DOI":"10.1016\/j.cellbi.2009.06.012","type":"journal-article","created":{"date-parts":[[2009,6,13]],"date-time":"2009-06-13T09:04:59Z","timestamp":1244883899000},"page":"926-933","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Inhibition of <i>PKHD1<\/i> may cause S\u2010phase entry via mTOR signaling 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