{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:12:44Z","timestamp":1790921564657,"version":"4.1.0"},"reference-count":33,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cellular Immunology"],"published-print":{"date-parts":[[2009]]},"DOI":"10.1016\/j.cellimm.2009.04.002","type":"journal-article","created":{"date-parts":[[2009,5,9]],"date-time":"2009-05-09T04:09:53Z","timestamp":1241842193000},"page":"131-137","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"title":["Increased per cell IFN-\u03b3 productivity indicates recent in vivo activation of T cells"],"prefix":"10.1016","volume":"258","author":[{"given":"Tobias R.","family":"Schlingmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carey L.","family":"Shive","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleg S.","family":"Targoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Tary-Lehmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul V.","family":"Lehmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cellimm.2009.04.002_bib1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S1074-7613(00)80469-0","article-title":"Massive expansion of antigen-specific CD8+ T cells during an acute virus infection","volume":"8","author":"Butz","year":"1998","journal-title":"Immunity"},{"key":"10.1016\/j.cellimm.2009.04.002_bib2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1126\/science.280.5361.227","article-title":"The numbers game for virus-specific CD8+ T cells","volume":"280","author":"Doherty","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.cellimm.2009.04.002_bib3","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1038\/nri1416","article-title":"Seventeen-colour flow cytometry: unravelling the immune system","volume":"4","author":"Perfetto","year":"2004","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib4","doi-asserted-by":"crossref","first-page":"4781","DOI":"10.1182\/blood-2005-12-4818","article-title":"HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells","volume":"107","author":"Betts","year":"2006","journal-title":"Blood"},{"key":"10.1016\/j.cellimm.2009.04.002_bib5","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1038\/nm1592","article-title":"Multifunctional TH1 cells define a correlate of vaccine-mediated protection against Leishmania major","volume":"13","author":"Darrah","year":"2007","journal-title":"Nat. Med."},{"key":"10.1016\/j.cellimm.2009.04.002_bib6","doi-asserted-by":"crossref","first-page":"8468","DOI":"10.1128\/JVI.00228-07","article-title":"Multiple-cytokine-producing antiviral CD4 T cells are functionally superior to single-cytokine-producing cells","volume":"81","author":"Kannanganat","year":"2007","journal-title":"J. Virol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib7","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1084\/jem.20062363","article-title":"Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses","volume":"204","author":"Precopio","year":"2007","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.cellimm.2009.04.002_bib8","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1084\/jem.20052246","article-title":"Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturation","volume":"203","author":"Casazza","year":"2006","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.cellimm.2009.04.002_bib9","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1111\/j.0105-2896.2006.00395.x","article-title":"Functional signatures of protective antiviral T-cell immunity in human virus infections","volume":"211","author":"Harari","year":"2006","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.cellimm.2009.04.002_bib10","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s00281-006-0025-4","article-title":"Polyfunctional analysis of human T cell responses: importance in vaccine immunogenicity and natural infection","volume":"28","author":"Makedonas","year":"2006","journal-title":"Springer Semin. Immunopathol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib11","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nri1840","article-title":"Functional signatures in antiviral T-cell immunity for monitoring virus-associated diseases","volume":"6","author":"Pantaleo","year":"2006","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib12","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1084\/jem.20032083","article-title":"Distinct time effects of vaccination on long-term proliferative and IFN-gamma-producing T cell memory to smallpox in humans","volume":"199","author":"Combadiere","year":"2004","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.cellimm.2009.04.002_bib13","doi-asserted-by":"crossref","first-page":"4969","DOI":"10.4049\/jimmunol.171.10.4969","article-title":"Cutting edge: long-term B cell memory in humans after smallpox vaccination","volume":"171","author":"Crotty","year":"2003","journal-title":"J. Immunol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib14","doi-asserted-by":"crossref","first-page":"2627","DOI":"10.1128\/jvi.70.4.2627-2631.1996","article-title":"Human cytotoxic T-cell memory: long-lived responses to vaccinia virus","volume":"70","author":"Demkowicz","year":"1996","journal-title":"J. Virol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib15","doi-asserted-by":"crossref","first-page":"3295","DOI":"10.1001\/jama.289.24.3295","article-title":"Response to smallpox vaccine in persons immunized in the distant past","volume":"289","author":"Frey","year":"2003","journal-title":"JAMA"},{"key":"10.1016\/j.cellimm.2009.04.002_bib16","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1038\/nm917","article-title":"Duration of antiviral immunity after smallpox vaccination","volume":"9","author":"Hammarlund","year":"2003","journal-title":"Nat. Med."},{"key":"10.1016\/j.cellimm.2009.04.002_bib17","doi-asserted-by":"crossref","first-page":"6339","DOI":"10.1128\/JVI.00116-06","article-title":"Obligatory requirement for antibody in recovery from a primary poxvirus infection","volume":"80","author":"Chaudhri","year":"2006","journal-title":"J. Virol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib18","doi-asserted-by":"crossref","first-page":"6829","DOI":"10.4049\/jimmunol.175.10.6829","article-title":"Antibodies and CD8+ T cells are complementary and essential for natural resistance to a highly lethal cytopathic virus","volume":"175","author":"Fang","year":"2005","journal-title":"J. Immunol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib19","doi-asserted-by":"crossref","first-page":"8301","DOI":"10.1128\/jvi.70.12.8301-8309.1996","article-title":"Different roles for CD4+ and CD8+ T lymphocytes and macrophage subsets in the control of a generalized virus infection","volume":"70","author":"Karupiah","year":"1996","journal-title":"J. Virol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib20","doi-asserted-by":"crossref","first-page":"6265","DOI":"10.4049\/jimmunol.172.10.6265","article-title":"Cellular and humoral immunity against vaccinia virus infection of mice","volume":"172","author":"Xu","year":"2004","journal-title":"J. Immunol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib21","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1086\/505224","article-title":"HLA-A2-restricted human CD8(+) cytotoxic T lymphocyte responses to a novel epitope in vaccinia virus that is conserved among orthopox viruses","volume":"194","author":"Dong","year":"2006","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.cellimm.2009.04.002_bib22","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1073\/pnas.262668999","article-title":"Identification of vaccinia virus epitope-specific HLA-A*0201-restricted T cells and comparative analysis of smallpox vaccines","volume":"100","author":"Drexler","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cellimm.2009.04.002_bib23","doi-asserted-by":"crossref","first-page":"13980","DOI":"10.1073\/pnas.0506768102","article-title":"HLA class I-restricted responses to vaccinia recognize a broad array of proteins mainly involved in virulence and viral gene regulation","volume":"102","author":"Oseroff","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cellimm.2009.04.002_bib24","doi-asserted-by":"crossref","first-page":"7052","DOI":"10.1128\/JVI.78.13.7052-7060.2004","article-title":"Protection against lethal vaccinia virus challenge in HLA-A2 transgenic mice by immunization with a single CD8+ T-cell peptide epitope of vaccinia and variola viruses","volume":"78","author":"Snyder","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib25","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1084\/jem.20022222","article-title":"Quantitation of CD8+ T cell responses to newly identified HLA-A*0201-restricted T cell epitopes conserved among vaccinia and variola (smallpox) viruses","volume":"197","author":"Terajima","year":"2003","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.cellimm.2009.04.002_bib26","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1084\/jem.182.5.1357","article-title":"Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations","volume":"182","author":"Openshaw","year":"1995","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.cellimm.2009.04.002_bib27","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S1074-7613(00)80470-7","article-title":"Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection","volume":"8","author":"Murali-Krishna","year":"1998","journal-title":"Immunity"},{"key":"10.1016\/j.cellimm.2009.04.002_bib28","doi-asserted-by":"crossref","first-page":"3723","DOI":"10.4049\/jimmunol.164.7.3723","article-title":"Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients","volume":"164","author":"Helms","year":"2000","journal-title":"J. Immunol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib29","doi-asserted-by":"crossref","first-page":"4598","DOI":"10.4049\/jimmunol.174.8.4598","article-title":"Does the frequency and avidity spectrum of the neuroantigen-specific T cells in the blood mirror the autoimmune process in the central nervous system of mice undergoing experimental allergic encephalomyelitis?","volume":"174","author":"Hofstetter","year":"2005","journal-title":"J. Immunol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib30","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0022-1759(03)00240-0","article-title":"Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine","volume":"279","author":"Guerkov","year":"2003","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.cellimm.2009.04.002_bib31","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.4049\/jimmunol.167.3.1353","article-title":"A T cell clone\u2019s avidity is a function of its activation state","volume":"167","author":"Hesse","year":"2001","journal-title":"J. Immunol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib32","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.clim.2007.05.012","article-title":"The secretory IFN-gamma response of single CD4 memory cells after activation on different antigen presenting cell types","volume":"124","author":"Ott","year":"2007","journal-title":"Clin. Immunol."},{"key":"10.1016\/j.cellimm.2009.04.002_bib33","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1146\/annurev.immunol.25.022106.141548","article-title":"Effector and memory CTL differentiation","volume":"25","author":"Williams","year":"2007","journal-title":"Annu. Rev. Immunol."}],"container-title":["Cellular Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0008874909000628?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0008874909000628?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T22:43:19Z","timestamp":1757803399000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0008874909000628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009]]}},"alternative-id":["S0008874909000628"],"URL":"https:\/\/doi.org\/10.1016\/j.cellimm.2009.04.002","relation":{},"ISSN":["0008-8749"],"issn-type":[{"value":"0008-8749","type":"print"}],"subject":[],"published":{"date-parts":[[2009]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Increased per cell IFN-\u03b3 productivity indicates recent in vivo activation of T cells","name":"articletitle","label":"Article Title"},{"value":"Cellular Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cellimm.2009.04.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2009 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}