{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:15:48Z","timestamp":1793254548804,"version":"4.5.0"},"reference-count":48,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T00:00:00Z","timestamp":1465516800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Foundation","doi-asserted-by":"publisher","award":["HHSN272200900041C"],"award-info":[{"award-number":["HHSN272200900041C"]}],"id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005014","name":"Northrup Grumman Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"NIAID, NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008510","name":"UMMS Center for Clinical and Translational Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008510","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2016,6]]},"DOI":"10.1016\/j.celrep.2016.06.028","type":"journal-article","created":{"date-parts":[[2016,6,27]],"date-time":"2016-06-27T13:00:37Z","timestamp":1467032437000},"page":"232-246","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":377,"title":["Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics"],"prefix":"10.1016","volume":"16","author":[{"given":"George","family":"Savidis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William M.","family":"McDougall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Meraner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill M.","family":"Perreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jocelyn M.","family":"Portmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaia","family":"Trincucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinu P.","family":"John","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron M.","family":"Aker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Renzette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas R.","family":"Robbins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiru","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharone","family":"Green","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy F.","family":"Kowalik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham L.","family":"Brass","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2016.06.028_bib1","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2016.06.028_bib2","doi-asserted-by":"crossref","first-page":"266","DOI":"10.3389\/fmicb.2014.00266","article-title":"Stress responses in flavivirus-infected cells: activation of unfolded protein response and autophagy","volume":"5","author":"Bl\u00e1zquez","year":"2014","journal-title":"Front Microbiol"},{"key":"10.1016\/j.celrep.2016.06.028_bib3","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.3390\/v7041927","article-title":"Insect-specific flaviviruses: a systematic review of their discovery, host range, mode of transmission, superinfection exclusion potential and genomic organization","volume":"7","author":"Blitvich","year":"2015","journal-title":"Viruses"},{"key":"10.1016\/j.celrep.2016.06.028_bib4","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1038\/nrm3588","article-title":"Organization of the ER-Golgi interface for membrane traffic control","volume":"14","author":"Brandizzi","year":"2013","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.celrep.2016.06.028_bib5","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1016\/j.celrep.2015.10.036","article-title":"Direct Visualization of HIV-1 Replication Intermediates Shows that Capsid and CPSF6 Modulate HIV-1 Intra-nuclear Invasion and Integration","volume":"13","author":"Chin","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2016.06.028_bib6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/ncb2383","article-title":"Defining human ERAD networks through an integrative mapping strategy","volume":"14","author":"Christianson","year":"2012","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.celrep.2016.06.028_bib7","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nature18296","article-title":"The Brazilian Zika virus strain causes birth defects in experimental models","volume":"534","author":"Cugola","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2016.06.028_bib8","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1056\/NEJMc1604449","article-title":"Evidence of Sexual Transmission of Zika Virus","volume":"374","author":"D\u2019Ortenzio","year":"2016","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.celrep.2016.06.028_bib9","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.1056\/NEJMoa1601824","article-title":"Zika Virus Infection with Prolonged Maternal Viremia and Fetal Brain Abnormalities","volume":"374","author":"Driggers","year":"2016","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.celrep.2016.06.028_bib10","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1038\/nmeth1006-777","article-title":"Minimizing the risk of reporting false positives in large-scale RNAi screens","volume":"3","author":"Echeverri","year":"2006","journal-title":"Nat. Methods"},{"key":"10.1016\/j.celrep.2016.06.028_bib11","doi-asserted-by":"crossref","first-page":"e1002337","DOI":"10.1371\/journal.ppat.1002337","article-title":"IFITM3 inhibits influenza A virus infection by preventing cytosolic entry","volume":"7","author":"Feeley","year":"2011","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2016.06.028_bib12","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.chom.2009.04.001","article-title":"Pathogenesis of flavivirus infections: using and abusing the host cell","volume":"5","author":"Fernandez-Garcia","year":"2009","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.celrep.2016.06.028_bib13","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01956-15","article-title":"Vaccine and Wild-Type Strains of Yellow Fever Virus Engage Distinct Entry Mechanisms and Differentially Stimulate Antiviral Immune Responses","volume":"7","author":"Fernandez-Garcia","year":"2016","journal-title":"MBio"},{"key":"10.1016\/j.celrep.2016.06.028_bib14","doi-asserted-by":"crossref","first-page":"8880","DOI":"10.1128\/JVI.00354-15","article-title":"Biology of Zika Virus Infection in Human Skin Cells","volume":"89","author":"Hamel","year":"2015","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2016.06.028_bib15","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1056\/NEJMp1603734","article-title":"The Zika Challenge","volume":"374","author":"Haug","year":"2016","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.celrep.2016.06.028_bib16","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1016\/j.cell.2014.05.010","article-title":"Development and applications of CRISPR-Cas9 for genome engineering","volume":"157","author":"Hsu","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2016.06.028_bib17","doi-asserted-by":"crossref","first-page":"4703","DOI":"10.3390\/v6114703","article-title":"Flavivirus-mosquito interactions","volume":"6","author":"Huang","year":"2014","journal-title":"Viruses"},{"key":"10.1016\/j.celrep.2016.06.028_bib18","doi-asserted-by":"crossref","first-page":"e1003232","DOI":"10.1371\/journal.ppat.1003232","article-title":"TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine","volume":"9","author":"Jemielity","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2016.06.028_bib19","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1126\/science.1167983","article-title":"Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum","volume":"323","author":"Jonikas","year":"2009","journal-title":"Science"},{"key":"10.1016\/j.celrep.2016.06.028_bib20","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/ncb2652","article-title":"A human genome-wide screen for regulators of clathrin-coated vesicle formation reveals an unexpected role for the V-ATPase","volume":"15","author":"Kozik","year":"2013","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.celrep.2016.06.028_bib21","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1038\/nature07207","article-title":"RNA interference screen for human genes associated with West Nile virus infection","volume":"455","author":"Krishnan","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2016.06.028_bib22","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.antiviral.2014.07.006","article-title":"Kinome siRNA screen identifies novel cell-type specific dengue host target genes","volume":"110","author":"Kwon","year":"2014","journal-title":"Antiviral Res."},{"key":"10.1016\/j.celrep.2016.06.028_bib23","doi-asserted-by":"crossref","first-page":"e1001969","DOI":"10.1371\/journal.pbio.1001969","article-title":"A conserved endoplasmic reticulum membrane protein complex (EMC) facilitates phospholipid transfer from the ER to mitochondria","volume":"12","author":"Lahiri","year":"2014","journal-title":"PLoS Biol."},{"key":"10.1016\/j.celrep.2016.06.028_bib24","doi-asserted-by":"crossref","first-page":"4864","DOI":"10.1128\/JVI.00252-16","article-title":"Zika Virus: New Clinical Syndromes and Its Emergence in the Western Hemisphere","volume":"90","author":"Lazear","year":"2016","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2016.06.028_bib25","doi-asserted-by":"crossref","first-page":"e1820","DOI":"10.1371\/journal.pntd.0001820","article-title":"G protein-coupled receptor kinase 2 promotes flaviviridae entry and replication","volume":"6","author":"Le Sommer","year":"2012","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.celrep.2016.06.028_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.stem.2016.04.017","article-title":"Zika virus disrupts neural progenitor development and leads to microcephaly in mice","volume":"19","author":"Li","year":"2016","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.celrep.2016.06.028_bib27","doi-asserted-by":"crossref","first-page":"20380","DOI":"10.1073\/pnas.0810485105","article-title":"Highly parallel identification of essential genes in cancer cells","volume":"105","author":"Luo","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2016.06.028_bib28","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.celrep.2015.06.049","article-title":"A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death","volume":"12","author":"Ma","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2016.06.028_bib29","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.chom.2012.08.009","article-title":"The TIM and TAM families of phosphatidylserine receptors mediate dengue virus entry","volume":"12","author":"Meertens","year":"2012","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.celrep.2016.06.028_bib30","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.stem.2016.04.009","article-title":"Understanding How Zika Virus Enters and Infects Neural Target Cells","volume":"18","author":"Miner","year":"2016","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.celrep.2016.06.028_bib31","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1016\/j.cell.2016.05.008","article-title":"Zika virus infection during pregnancy in mice causes placental damage and fetal demise","volume":"165","author":"Miner","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2016.06.028_bib32","doi-asserted-by":"crossref","first-page":"4275","DOI":"10.1128\/JVI.03287-13","article-title":"Role of phosphatidylserine receptors in enveloped virus infection","volume":"88","author":"Morizono","year":"2014","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2016.06.028_bib33","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1128\/CMR.00072-15","article-title":"Zika Virus","volume":"29","author":"Musso","year":"2016","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.celrep.2016.06.028_bib34","doi-asserted-by":"crossref","first-page":"14226","DOI":"10.1074\/jbc.M111.222703","article-title":"Dengue virus modulates the unfolded protein response in a time-dependent manner","volume":"286","author":"Pe\u00f1a","year":"2011","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2016.06.028_bib35","doi-asserted-by":"crossref","first-page":"e38202","DOI":"10.1371\/journal.pone.0038202","article-title":"Early dengue virus protein synthesis induces extensive rearrangement of the endoplasmic reticulum independent of the UPR and SREBP-2 pathway","volume":"7","author":"Pe\u00f1a","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.celrep.2016.06.028_bib36","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3390\/v6010069","article-title":"Flavivirus entry receptors: an update","volume":"6","author":"Perera-Lecoin","year":"2014","journal-title":"Viruses"},{"key":"10.1016\/j.celrep.2016.06.028_bib37","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/j.celrep.2015.06.076","article-title":"RNASEK Is a V-ATPase-Associated Factor Required for Endocytosis and the Replication of Rhinovirus, Influenza A Virus, and Dengue Virus","volume":"12","author":"Perreira","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2016.06.028_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.aivir.2015.11.001","article-title":"Functional Genomic Strategies for Elucidating Human-Virus Interactions: Will CRISPR Knockout RNAi and Haploid Cells?","volume":"94","author":"Perreira","year":"2016","journal-title":"Adv. Virus Res."},{"key":"10.1016\/j.celrep.2016.06.028_bib39","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/nature06384","article-title":"Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes","volume":"450","author":"Rapoport","year":"2007","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2016.06.028_bib40","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1056\/NEJMsr1604338","article-title":"Zika Virus and Birth Defects--Reviewing the Evidence for Causality","volume":"374","author":"Rasmussen","year":"2016","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.celrep.2016.06.028_bib41","doi-asserted-by":"crossref","first-page":"E1055","DOI":"10.1073\/pnas.1216154110","article-title":"Biosynthesis of ionotropic acetylcholine receptors requires the evolutionarily conserved ER membrane complex","volume":"110","author":"Richard","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2016.06.028_bib42","doi-asserted-by":"crossref","first-page":"14682","DOI":"10.1073\/pnas.1508095112","article-title":"Virion-associated phosphatidylethanolamine promotes TIM1-mediated infection by Ebola, dengue, and West Nile viruses","volume":"112","author":"Richard","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2016.06.028_bib43","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/nmeth.3047","article-title":"Improved vectors and genome-wide libraries for CRISPR screening","volume":"11","author":"Sanjana","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1016\/j.celrep.2016.06.028_bib44","doi-asserted-by":"crossref","first-page":"4","DOI":"10.7554\/eLife.06306","article-title":"dPob\/EMC is essential for biosynthesis of rhodopsin and other multi-pass membrane proteins in Drosophila photoreceptors","volume":"4","author":"Satoh","year":"2015","journal-title":"eLife"},{"key":"10.1016\/j.celrep.2016.06.028_bib45","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1038\/nature07967","article-title":"Discovery of insect and human dengue virus host factors","volume":"458","author":"Sessions","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2016.06.028_bib46","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1247005","article-title":"Genome-scale CRISPR-Cas9 knockout screening in human cells","volume":"343","author":"Shalem","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.celrep.2016.06.028_bib47","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/nrg3899","article-title":"High-throughput functional genomics using CRISPR-Cas9","volume":"16","author":"Shalem","year":"2015","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.celrep.2016.06.028_bib48","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.celrep.2014.09.031","article-title":"Comprehensive identification of host modulators of HIV-1 replication using multiple orthologous RNAi reagents","volume":"9","author":"Zhu","year":"2014","journal-title":"Cell Rep."}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124716307689?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124716307689?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,6,2]],"date-time":"2024-06-02T16:02:07Z","timestamp":1717344127000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124716307689"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["S2211124716307689"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2016.06.028","relation":{},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2016,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2016.06.028","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 The Author(s).","name":"copyright","label":"Copyright"}]}}