{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:10:25Z","timestamp":1790071825254,"version":"4.0.1"},"reference-count":49,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2018,3,13]],"date-time":"2018-03-13T00:00:00Z","timestamp":1520899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI123359"],"award-info":[{"award-number":["R01AI123359"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI127775"],"award-info":[{"award-number":["R01AI127775"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1 TR000430"],"award-info":[{"award-number":["UL1 TR000430"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007234","name":"University of Chicago","doi-asserted-by":"publisher","award":["R01AI123359-02S1"],"award-info":[{"award-number":["R01AI123359-02S1"]}],"id":[{"id":"10.13039\/100007234","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007234","name":"University of Chicago","doi-asserted-by":"publisher","award":["T32GM007183"],"award-info":[{"award-number":["T32GM007183"]}],"id":[{"id":"10.13039\/100007234","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2018,4]]},"DOI":"10.1016\/j.celrep.2018.03.045","type":"journal-article","created":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T11:42:19Z","timestamp":1523360539000},"page":"596-607","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":275,"title":["Genome-wide CRISPR\/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication"],"prefix":"10.1016","volume":"23","author":[{"given":"Julianna","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine T.","family":"Perez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asiel","family":"Benitez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheswaran","family":"Kandasamy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoontae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"tenOever","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balaji","family":"Manicassamy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2018.03.045_bib1","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1242\/dev.057729","article-title":"Capicua DNA-binding sites are general response elements for RTK signaling in Drosophila","volume":"138","author":"Ajuria","year":"2011","journal-title":"Development"},{"key":"10.1016\/j.celrep.2018.03.045_bib2","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1073\/pnas.1524498113","article-title":"Enterovirus D68 receptor requirements unveiled by haploid genetics","volume":"113","author":"Baggen","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2018.03.045_bib3","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1016\/j.celrep.2015.05.032","article-title":"In\u00a0vivo RNAi screening identifies MDA5 as a significant contributor to the cellular defense against influenza A virus","volume":"11","author":"Benitez","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2018.03.045_bib4","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1126\/science.1210557","article-title":"Mutations in CIC and FUBP1 contribute to human oligodendroglioma","volume":"333","author":"Bettegowda","year":"2011","journal-title":"Science"},{"key":"10.1016\/j.celrep.2018.03.045_bib5","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1016\/j.cell.2009.12.017","article-title":"The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus","volume":"139","author":"Brass","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2018.03.045_bib6","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1038\/ncb2112","article-title":"SUMOylation of the GTPase Rac1 is required for optimal cell migration","volume":"12","author":"Castillo-Lluva","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.celrep.2018.03.045_bib7","doi-asserted-by":"crossref","first-page":"18153","DOI":"10.1073\/pnas.0405172102","article-title":"Influenza virus entry and infection require host cell N-linked glycoprotein","volume":"101","author":"Chu","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2018.03.045_bib8","doi-asserted-by":"crossref","first-page":"7457","DOI":"10.1073\/pnas.1200987109","article-title":"Influenza A virus entry into cells lacking sialylated N-glycans","volume":"109","author":"de Vries","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2018.03.045_bib9","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1042\/BJ20101562","article-title":"ERK\/p90(RSK)\/14-3-3 signalling has an impact on expression of PEA3 Ets transcription factors via the transcriptional repressor capic\u00faa","volume":"433","author":"Dissanayake","year":"2011","journal-title":"Biochem. J."},{"key":"10.1016\/j.celrep.2018.03.045_bib10","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.csbj.2014.05.003","article-title":"Structure and function of nucleotide sugar transporters: current progress","volume":"10","author":"Hadley","year":"2014","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"10.1016\/j.celrep.2018.03.045_bib11","doi-asserted-by":"crossref","first-page":"E6248","DOI":"10.1073\/pnas.1605575113","article-title":"Functional receptor molecules CD300lf and CD300ld within the CD300 family enable murine noroviruses to infect cells","volume":"113","author":"Haga","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2018.03.045_bib12","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1038\/nature07151","article-title":"Drosophila RNAi screen identifies host genes important for influenza virus replication","volume":"454","author":"Hao","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2018.03.045_bib13","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1242\/jcs.092965","article-title":"The Capicua repressor--a general sensor of RTK signaling in development and disease","volume":"125","author":"Jim\u00e9nez","year":"2012","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2018.03.045_bib14","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/nature08760","article-title":"Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication","volume":"463","author":"Karlas","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2018.03.045_bib15","doi-asserted-by":"crossref","first-page":"8272","DOI":"10.1038\/srep08272","article-title":"Deficiency of Capicua disrupts bile acid homeostasis","volume":"5","author":"Kim","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.celrep.2018.03.045_bib16","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/nature08699","article-title":"Human host factors required for influenza virus replication","volume":"463","author":"K\u00f6nig","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2018.03.045_bib17","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1016\/j.cell.2006.11.038","article-title":"ATAXIN-1 interacts with the repressor Capicua in its native complex to cause SCA1 neuropathology","volume":"127","author":"Lam","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2018.03.045_bib18","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.devcel.2011.08.017","article-title":"ATXN1 protein family and CIC regulate extracellular matrix remodeling and lung alveolarization","volume":"21","author":"Lee","year":"2011","journal-title":"Dev. Cell"},{"key":"10.1016\/j.celrep.2018.03.045_bib19","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1186\/s13059-014-0554-4","article-title":"MAGeCK enables robust identification of essential genes from genome-scale CRISPR\/Cas9 knockout screens","volume":"15","author":"Li","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.celrep.2018.03.045_bib20","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.celrep.2015.06.049","article-title":"A CRISPR-based screen identifies genes essential for West-Nile-virus-induced cell death","volume":"12","author":"Ma","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2018.03.045_bib21","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/nature18631","article-title":"Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens","volume":"535","author":"Marceau","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2018.03.045_bib22","doi-asserted-by":"crossref","first-page":"566","DOI":"10.3390\/v2020566","article-title":"A host of factors regulating influenza virus replication","volume":"2","author":"Mehle","year":"2010","journal-title":"Viruses"},{"key":"10.1016\/j.celrep.2018.03.045_bib23","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/S0014-5793(02)03486-5","article-title":"PIAS3 induces SUMO-1 modification and transcriptional repression of IRF-1","volume":"530","author":"Nakagawa","year":"2002","journal-title":"FEBS Lett."},{"key":"10.1016\/j.celrep.2018.03.045_bib24","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/ng.3728","article-title":"Inactivation of Capicua drives cancer metastasis","volume":"49","author":"Okimoto","year":"2017","journal-title":"Nat. Genet."},{"key":"10.1016\/j.celrep.2018.03.045_bib25","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1126\/science.aaf1220","article-title":"Discovery of a proteinaceous cellular receptor for a norovirus","volume":"353","author":"Orchard","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.celrep.2018.03.045_bib26","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1038\/ng.3741","article-title":"A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors","volume":"49","author":"Park","year":"2017","journal-title":"Nat. Genet."},{"key":"10.1016\/j.celrep.2018.03.045_bib27","doi-asserted-by":"crossref","first-page":"16037","DOI":"10.1038\/ncomms16037","article-title":"Capicua deficiency induces autoimmunity and promotes follicular helper T\u00a0cell differentiation via derepression of ETV5","volume":"8","author":"Park","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.celrep.2018.03.045_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.aivir.2015.11.001","article-title":"functional genomic strategies for elucidating human-virus interactions: will CRISPR knockout RNAi and haploid cells?","volume":"94","author":"Perreira","year":"2016","journal-title":"Adv. Virus Res."},{"key":"10.1016\/j.celrep.2018.03.045_bib29","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1515\/BC.2008.145","article-title":"RNA viruses and the mitogenic Raf\/MEK\/ERK signal transduction cascade","volume":"389","author":"Pleschka","year":"2008","journal-title":"Biol. Chem."},{"key":"10.1016\/j.celrep.2018.03.045_bib30","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1038\/nchembio.999","article-title":"Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome","volume":"8","author":"Rillahan","year":"2012","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.celrep.2018.03.045_bib31","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/nmeth.3047","article-title":"Improved vectors and genome-wide libraries for CRISPR screening","volume":"11","author":"Sanjana","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1016\/j.celrep.2018.03.045_bib32","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.celrep.2016.06.028","article-title":"Identification of Zika virus and dengue Virus dependency factors using functional genomics","volume":"16","author":"Savidis","year":"2016","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2018.03.045_bib33","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1247005","article-title":"Genome-scale CRISPR-Cas9 knockout screening in human cells","volume":"343","author":"Shalem","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.celrep.2018.03.045_bib34","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.cell.2009.12.018","article-title":"A physical and regulatory map of host-influenza interactions reveals pathways in H1N1 infection","volume":"139","author":"Shapira","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2018.03.045_bib35","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.micinf.2011.01.012","article-title":"Uncovering the global host cell requirements for influenza virus replication via RNAi screening","volume":"13","author":"Stertz","year":"2011","journal-title":"Microbes Infect."},{"key":"10.1016\/j.celrep.2018.03.045_bib36","doi-asserted-by":"crossref","first-page":"17516","DOI":"10.1073\/pnas.1312374110","article-title":"Pooled RNAi screen identifies ubiquitin ligase Itch as crucial for influenza A virus release from the endosome during virus entry","volume":"110","author":"Su","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2018.03.045_bib37","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.virol.2009.02.046","article-title":"The use of random homozygous gene perturbation to identify novel host-oriented targets for influenza","volume":"387","author":"Sui","year":"2009","journal-title":"Virology"},{"key":"10.1016\/j.celrep.2018.03.045_bib38","doi-asserted-by":"crossref","first-page":"2673","DOI":"10.1242\/dev.148767","article-title":"COP9 signalosome subunits protect Capicua from MAPK-dependent and -independent mechanisms of degradation","volume":"144","author":"Suisse","year":"2017","journal-title":"Development"},{"key":"10.1016\/j.celrep.2018.03.045_bib39","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/j.chom.2015.11.002","article-title":"Meta- and orthogonal integration of influenza \u201cOMICs\u201d data defines a role for UBR4 in virus budding","volume":"18","author":"Tripathi","year":"2015","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.celrep.2018.03.045_bib40","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.jviromet.2009.10.020","article-title":"An enzymatic virus-like particle assay for sensitive detection of virus entry","volume":"163","author":"Tscherne","year":"2010","journal-title":"J.\u00a0Virol. Methods"},{"key":"10.1016\/j.celrep.2018.03.045_bib41","doi-asserted-by":"crossref","first-page":"6182","DOI":"10.1128\/JVI.03024-12","article-title":"Tyrosine 132 phosphorylation of influenza A virus M1 protein is crucial for virus replication by controlling the nuclear import of M1","volume":"87","author":"Wang","year":"2013","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2018.03.045_bib42","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1126\/science.1246981","article-title":"Genetic screens in human cells using the CRISPR-Cas9 system","volume":"343","author":"Wang","year":"2014","journal-title":"Science"},{"key":"10.1016\/j.celrep.2018.03.045_bib43","doi-asserted-by":"crossref","first-page":"e39284","DOI":"10.1371\/journal.pone.0039284","article-title":"Host modulators of H1N1 cytopathogenicity","volume":"7","author":"Ward","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.celrep.2018.03.045_bib44","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.chom.2010.05.008","article-title":"Cellular networks involved in the influenza virus life cycle","volume":"7","author":"Watanabe","year":"2010","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.celrep.2018.03.045_bib45","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/j.chom.2014.11.002","article-title":"Influenza virus-host interactome screen as a platform for antiviral drug development","volume":"16","author":"Watanabe","year":"2014","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.celrep.2018.03.045_bib46","series-title":"Fields Virology","article-title":"Orthomyxoviruses.","author":"Wright","year":"2013"},{"key":"10.1016\/j.celrep.2018.03.045_bib47","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.cell.2015.12.035","article-title":"Biology and applications of CRISPR systems: harnessing nature\u2019s toolbox for genome engineering","volume":"164","author":"Wright","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2018.03.045_bib48","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1039\/C5MB00663E","article-title":"ReactomePA: an R\/Bioconductor package for reactome pathway analysis and visualization","volume":"12","author":"Yu","year":"2016","journal-title":"Mol. Biosyst."},{"key":"10.1016\/j.celrep.2018.03.045_bib49","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1038\/nature18625","article-title":"A CRISPR screen defines a signal peptide processing pathway required by flaviviruses","volume":"535","author":"Zhang","year":"2016","journal-title":"Nature"}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124718303875?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124718303875?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T10:19:21Z","timestamp":1761905961000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124718303875"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["S2211124718303875"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2018.03.045","relation":{},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2018,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Genome-wide CRISPR\/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2018.03.045","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Author(s).","name":"copyright","label":"Copyright"}]}}