{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:45:00Z","timestamp":1790829900054,"version":"4.1.0"},"reference-count":36,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,3,26]],"date-time":"2019-03-26T00:00:00Z","timestamp":1553558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001697","name":"Keio University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001697","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2019,4]]},"DOI":"10.1016\/j.celrep.2019.03.088","type":"journal-article","created":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T11:03:38Z","timestamp":1556017418000},"page":"1265-1276.e4","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":222,"title":["Establishment of Patient-Derived Organoids and Drug Screening for Biliary Tract Carcinoma"],"prefix":"10.1016","volume":"27","author":[{"given":"Yoshimasa","family":"Saito","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihide","family":"Muramatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yae","family":"Kanai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidenori","family":"Ojima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aoi","family":"Sukeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyoshi","family":"Hiraoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eri","family":"Arai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Sugiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juntaro","family":"Matsuzaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoei","family":"Uchida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nao","family":"Yoshikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Furukawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetsugu","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2019.03.088_bib1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.stem.2009.11.013","article-title":"Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in\u00a0vitro","volume":"6","author":"Barker","year":"2010","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.celrep.2019.03.088_bib2","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1007\/s00535-013-0767-4","article-title":"KRAS mutation status is not predictive for objective response to anti-EGFR treatment with erlotinib in patients with advanced pancreatic cancer","volume":"48","author":"Boeck","year":"2013","journal-title":"J.\u00a0Gastroenterol."},{"key":"10.1016\/j.celrep.2019.03.088_bib3","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.cell.2014.12.021","article-title":"Organoid models of human and mouse ductal pancreatic cancer","volume":"160","author":"Boj","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2019.03.088_bib4","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1038\/nm.4438","article-title":"Human primary liver cancer-derived organoid cultures for disease modeling and drug screening","volume":"23","author":"Broutier","year":"2017","journal-title":"Nat. Med."},{"key":"10.1016\/j.celrep.2019.03.088_bib5","doi-asserted-by":"crossref","first-page":"11452","DOI":"10.1073\/pnas.1106083108","article-title":"R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt\/beta-catenin signaling","volume":"108","author":"Carmon","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2019.03.088_bib6","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/nature10337","article-title":"Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling","volume":"476","author":"de Lau","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2019.03.088_bib7","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1053\/j.gastro.2009.02.038","article-title":"Burden of digestive diseases in the United States Part III: liver, biliary tract, and pancreas","volume":"136","author":"Everhart","year":"2009","journal-title":"Gastroenterology"},{"key":"10.1016\/j.celrep.2019.03.088_bib8","doi-asserted-by":"crossref","first-page":"3082","DOI":"10.1093\/annonc\/mdt380","article-title":"Expression of TP53 mutation-associated microRNAs predicts clinical outcome in head and neck squamous cell carcinoma patients","volume":"24","author":"Ganci","year":"2013","journal-title":"Ann. Oncol."},{"key":"10.1016\/j.celrep.2019.03.088_bib9","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.cell.2014.08.016","article-title":"Organoid cultures derived from patients with advanced prostate cancer","volume":"159","author":"Gao","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2019.03.088_bib10","first-page":"4670","article-title":"Allelotype of pancreatic adenocarcinoma using xenograft enrichment","volume":"55","author":"Hahn","year":"1995","journal-title":"Cancer Res."},{"key":"10.1016\/j.celrep.2019.03.088_bib11","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1038\/emboj.2013.204","article-title":"Unlimited in\u00a0vitro expansion of adult bi-potent pancreas progenitors through the Lgr5\/R-spondin axis","volume":"32","author":"Huch","year":"2013","journal-title":"EMBO J."},{"key":"10.1016\/j.celrep.2019.03.088_bib12","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1038\/nature11826","article-title":"In\u00a0vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration","volume":"494","author":"Huch","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2019.03.088_bib13","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1007\/s11864-016-0432-2","article-title":"Genomic Profiling of Biliary Tract Cancers and Implications for Clinical Practice","volume":"17","author":"Jain","year":"2016","journal-title":"Curr. Treat. Options Oncol."},{"key":"10.1016\/j.celrep.2019.03.088_bib14","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.1002\/ijc.29610","article-title":"Genome-wide small noncoding RNA profiling of pediatric high-grade gliomas reveals deregulation of several miRNAs, identifies downregulation of snoRNA cluster HBII-52 and delineates H3F3A and TP53 mutant-specific miRNAs and snoRNAs","volume":"137","author":"Jha","year":"2015","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.celrep.2019.03.088_bib15","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1056\/NEJMoa0804385","article-title":"K-ras mutations and benefit from cetuximab in advanced colorectal cancer","volume":"359","author":"Karapetis","year":"2008","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.celrep.2019.03.088_bib16","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1038\/nm.3802","article-title":"Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids","volume":"21","author":"Matano","year":"2015","journal-title":"Nat. Med."},{"key":"10.1016\/j.celrep.2019.03.088_bib17","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1038\/cdd.2013.125","article-title":"The miR-17\/92 cluster: a comprehensive update on its genomics, genetics, functions and increasingly important and numerous roles in health and disease","volume":"20","author":"Mogilyansky","year":"2013","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.celrep.2019.03.088_bib18","doi-asserted-by":"crossref","first-page":"3120","DOI":"10.1096\/fj.08-109611","article-title":"A novel eGFP-expressing immunodeficient mouse model to study tumor-host interactions","volume":"22","author":"Niclou","year":"2008","journal-title":"FASEB J."},{"key":"10.1016\/j.celrep.2019.03.088_bib19","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1111\/j.1349-7006.2009.01462.x","article-title":"Establishment of six new human biliary tract carcinoma cell lines and identification of MAGEH1 as a candidate biomarker for predicting the efficacy of gemcitabine treatment","volume":"101","author":"Ojima","year":"2010","journal-title":"Cancer Sci."},{"key":"10.1016\/j.celrep.2019.03.088_bib20","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.cgh.2012.09.009","article-title":"Classification, diagnosis, and management of cholangiocarcinoma","volume":"11","author":"Razumilava","year":"2013","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"10.1016\/j.celrep.2019.03.088_bib21","doi-asserted-by":"crossref","first-page":"2168","DOI":"10.1016\/S0140-6736(13)61903-0","article-title":"Cholangiocarcinoma","volume":"383","author":"Razumilava","year":"2014","journal-title":"Lancet"},{"key":"10.1016\/j.celrep.2019.03.088_bib22","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1053\/j.gastro.2013.10.013","article-title":"Pathogenesis, diagnosis, and management of cholangiocarcinoma","volume":"145","author":"Rizvi","year":"2013","journal-title":"Gastroenterology"},{"key":"10.1016\/j.celrep.2019.03.088_bib23","doi-asserted-by":"crossref","first-page":"25311","DOI":"10.1038\/srep25311","article-title":"Inhibition of DNA Methylation Suppresses Intestinal Tumor Organoids by Inducing an Anti-Viral Response","volume":"6","author":"Saito","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.celrep.2019.03.088_bib24","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.1038\/s41598-018-21121-6","article-title":"Induction of differentiation of intrahepatic cholangiocarcinoma cells to functional hepatocytes using an organoid culture system","volume":"8","author":"Saito","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.celrep.2019.03.088_bib25","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1038\/nature07935","article-title":"Single Lgr5 stem cells build crypt-villus structures in\u00a0vitro without a mesenchymal niche","volume":"459","author":"Sato","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2019.03.088_bib26","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1053\/j.gastro.2011.07.050","article-title":"Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett\u2019s epithelium","volume":"141","author":"Sato","year":"2011","journal-title":"Gastroenterology"},{"key":"10.1016\/j.celrep.2019.03.088_bib27","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/nature09637","article-title":"Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts","volume":"469","author":"Sato","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2019.03.088_bib28","first-page":"4745","article-title":"Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan","volume":"7","author":"Sato","year":"2014","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"10.1016\/j.celrep.2019.03.088_bib29","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.stem.2017.12.009","article-title":"Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression","volume":"22","author":"Seino","year":"2018","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.celrep.2019.03.088_bib30","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1756-9966-31-55","article-title":"Potential utility of eGFP-expressing NOG mice (NOG-EGFP) as a high purity cancer sampling system","volume":"31","author":"Shima","year":"2012","journal-title":"J.\u00a0Exp. Clin. Cancer Res."},{"key":"10.1016\/j.celrep.2019.03.088_bib31","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.cell.2015.03.053","article-title":"Prospective derivation of a living organoid biobank of colorectal cancer patients","volume":"161","author":"van de Wetering","year":"2015","journal-title":"Cell"},{"issue":"Pt A","key":"10.1016\/j.celrep.2019.03.088_bib32","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.biocel.2016.06.004","article-title":"Aldehyde dehydrogenase 3A1 promotes multi-modality resistance and alters gene expression profile in human breast adenocarcinoma MCF-7 cells","volume":"77","author":"Voulgaridou","year":"2016","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"10.1016\/j.celrep.2019.03.088_bib33","doi-asserted-by":"crossref","first-page":"18162","DOI":"10.18632\/oncotarget.4216","article-title":"Erlotinib is effective in pancreatic cancer with epidermal growth factor receptor mutations: a randomized, open-label, prospective trial","volume":"6","author":"Wang","year":"2015","journal-title":"Oncotarget"},{"key":"10.1016\/j.celrep.2019.03.088_bib34","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/j.jhep.2018.01.009","article-title":"Genomic characterization of biliary tract cancers identifies driver genes and predisposing mutations","volume":"68","author":"Wardell","year":"2018","journal-title":"J.\u00a0Hepatol."},{"key":"10.1016\/j.celrep.2019.03.088_bib35","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1038\/emboj.2009.214","article-title":"Repression of the miR-17-92 cluster by p53 has an important function in hypoxia-induced apoptosis","volume":"28","author":"Yan","year":"2009","journal-title":"EMBO J."},{"key":"10.1016\/j.celrep.2019.03.088_bib36","doi-asserted-by":"crossref","first-page":"5696","DOI":"10.1038\/ncomms6696","article-title":"Mutational landscape of intrahepatic cholangiocarcinoma","volume":"5","author":"Zou","year":"2014","journal-title":"Nat. Commun."}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124719304279?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124719304279?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T10:39:11Z","timestamp":1761907151000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124719304279"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["S2211124719304279"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2019.03.088","relation":{},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2019,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Establishment of Patient-Derived Organoids and Drug Screening for Biliary Tract Carcinoma","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2019.03.088","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 The Author(s).","name":"copyright","label":"Copyright"}]}}