{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:08:00Z","timestamp":1791475680167,"version":"4.3.4"},"reference-count":93,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T00:00:00Z","timestamp":1582675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008732","name":"Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["16K19041"],"award-info":[{"award-number":["16K19041"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["22247030"],"award-info":[{"award-number":["22247030"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["23112003"],"award-info":[{"award-number":["23112003"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013236","name":"MSD Life Science Foundation, Public Interest Incorporated Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013236","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007428","name":"Naito Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2020,4]]},"DOI":"10.1016\/j.celrep.2020.02.088","type":"journal-article","created":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T08:05:17Z","timestamp":1586246717000},"page":"107407","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"title":["Shank2 Binds to aPKC and Controls Tight Junction Formation with Rap1 Signaling during Establishment of Epithelial Cell Polarity"],"prefix":"10.1016","volume":"31","author":[{"given":"Kazunori","family":"Sasaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Kojitani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Hirose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Yoshihama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidefumi","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayumi","family":"Takayanagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masa-aki","family":"Nakaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisashi","family":"Hirano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidehisa","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeo","family":"Ohno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2020.02.088_bib1","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1042\/bj3350417","article-title":"Atypical protein kinase Clambda binds and regulates p70 S6 kinase","volume":"335","author":"Akimoto","year":"1998","journal-title":"Biochem. J."},{"key":"10.1016\/j.celrep.2020.02.088_bib2","doi-asserted-by":"crossref","first-page":"e20795","DOI":"10.7554\/eLife.20795","article-title":"AMP-activated protein kinase fortifies epithelial tight junctions during energetic stress via its effector GIV\/Girdin","volume":"5","author":"Aznar","year":"2016","journal-title":"eLife"},{"key":"10.1016\/j.celrep.2020.02.088_bib3","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1038\/ng.589","article-title":"Mutations in the SHANK2 synaptic scaffolding gene in autism spectrum disorder and mental retardation","volume":"42","author":"Berkel","year":"2010","journal-title":"Nat. Genet."},{"key":"10.1016\/j.celrep.2020.02.088_bib4","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1093\/hmg\/ddr470","article-title":"Inherited and de novo SHANK2 variants associated with autism spectrum disorder impair neuronal morphogenesis and physiology","volume":"21","author":"Berkel","year":"2012","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.celrep.2020.02.088_bib5","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1083\/jcb.200311093","article-title":"Rap1 up-regulation and activation on plasma membrane regulates T\u00a0cell adhesion","volume":"164","author":"Bivona","year":"2004","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib6","doi-asserted-by":"crossref","first-page":"40104","DOI":"10.1074\/jbc.M102454200","article-title":"Synaptic scaffolding proteins in rat brain. Ankyrin repeats of the multidomain Shank protein family interact with the cytoskeletal protein alpha-fodrin","volume":"276","author":"B\u00f6ckers","year":"2001","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.02.088_bib7","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1046\/j.1471-4159.2002.00931.x","article-title":"ProSAP\/Shank proteins\u2014a family of higher order organizing molecules of the postsynaptic density with an emerging role in human neurological disease","volume":"81","author":"Boeckers","year":"2002","journal-title":"J.\u00a0Neurochem."},{"key":"10.1016\/j.celrep.2020.02.088_bib8","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1111\/j.1471-4159.2004.02910.x","article-title":"C-terminal synaptic targeting elements for postsynaptic density proteins ProSAP1\/Shank2 and ProSAP2\/Shank3","volume":"92","author":"Boeckers","year":"2005","journal-title":"J.\u00a0Neurochem."},{"key":"10.1016\/j.celrep.2020.02.088_bib9","doi-asserted-by":"crossref","first-page":"9064","DOI":"10.1073\/pnas.97.16.9064","article-title":"The junctional multidomain protein AF-6 is a binding partner of the Rap1A GTPase and associates with the actin cytoskeletal regulator profilin","volume":"97","author":"Boettner","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2020.02.088_bib10","doi-asserted-by":"crossref","first-page":"dev157941","DOI":"10.1242\/dev.157941","article-title":"Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment","volume":"145","author":"Bonello","year":"2018","journal-title":"Development"},{"key":"10.1016\/j.celrep.2020.02.088_bib11","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.ceb.2005.08.012","article-title":"The challenges of abundance: epithelial junctions and small GTPase signalling","volume":"17","author":"Braga","year":"2005","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib12","doi-asserted-by":"crossref","first-page":"12804","DOI":"10.1074\/jbc.M312171200","article-title":"Comprehensive proteomic analysis of human Par protein complexes reveals an interconnected protein network","volume":"279","author":"Brajenovic","year":"2004","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.02.088_bib13","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1038\/nrm2523","article-title":"From cells to organs: building polarized tissue","volume":"9","author":"Bryant","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib14","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1038\/ncb2106","article-title":"A molecular network for de novo generation of the apical surface and lumen","volume":"12","author":"Bryant","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib15","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.bbrc.2004.03.107","article-title":"KIBRA is a novel substrate for protein kinase Czeta","volume":"317","author":"B\u00fcther","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.celrep.2020.02.088_bib16","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1091\/mbc.e12-10-0736","article-title":"Rap1 and Canoe\/afadin are essential for establishment of apical-basal polarity in the Drosophila embryo","volume":"24","author":"Choi","year":"2013","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.celrep.2020.02.088_bib17","doi-asserted-by":"crossref","first-page":"e12510","DOI":"10.14814\/phy2.12510","article-title":"Shank2 regulates renal albumin endocytosis","volume":"3","author":"Dobrinskikh","year":"2015","journal-title":"Physiol. Rep."},{"key":"10.1016\/j.celrep.2020.02.088_bib18","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0092-8674(00)81278-7","article-title":"Origins of cell polarity","volume":"84","author":"Drubin","year":"1996","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.02.088_bib19","doi-asserted-by":"crossref","first-page":"5838","DOI":"10.1128\/MCB.18.10.5838","article-title":"Identification of a novel cortactin SH3 domain-binding protein and its localization to growth cones of cultured neurons","volume":"18","author":"Du","year":"1998","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.conb.2018.12.001","article-title":"Phase separation as a mechanism for assembling dynamic postsynaptic density signalling complexes","volume":"57","author":"Feng","year":"2019","journal-title":"Curr. Opin. Neurobiol."},{"key":"10.1016\/j.celrep.2020.02.088_bib21","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1101\/gr.2659105","article-title":"Protein interaction mapping: a Drosophila case study","volume":"15","author":"Formstecher","year":"2005","journal-title":"Genome Res."},{"key":"10.1016\/j.celrep.2020.02.088_bib22","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1074\/jbc.M411099200","article-title":"Involvement of the c-Src-Crk-C3G-Rap1 signaling in the nectin-induced activation of Cdc42 and formation of adherens junctions","volume":"280","author":"Fukuyama","year":"2005","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.02.088_bib23","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1083\/jcb.200608161","article-title":"The Par polarity complex regulates Rap1- and chemokine-induced T\u00a0cell polarization","volume":"176","author":"G\u00e9rard","year":"2007","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib24","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/BF01870778","article-title":"Tight junction formation in cultured epithelial cells (MDCK)","volume":"86","author":"Gonzalez-Mariscal","year":"1985","journal-title":"J.\u00a0Membr. Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib25","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.bbamem.2007.08.018","article-title":"Crosstalk of tight junction components with signaling pathways","volume":"1778","author":"Gonz\u00e1lez-Mariscal","year":"2008","journal-title":"Biochim. Biophys. Acta"},{"issue":"11 Pt A","key":"10.1016\/j.celrep.2020.02.088_bib26","doi-asserted-by":"crossref","first-page":"2404","DOI":"10.1016\/j.bbagen.2016.07.008","article-title":"Nicotine enhances the malignant potential of human pancreatic cancer cells via activation of atypical protein kinase C","volume":"1860","author":"Hanaki","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.celrep.2020.02.088_bib27","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1016\/j.cub.2010.09.032","article-title":"Par3 controls epithelial spindle orientation by aPKC-mediated phosphorylation of apical Pins","volume":"20","author":"Hao","year":"2010","journal-title":"Curr. Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib28","doi-asserted-by":"crossref","first-page":"5820","DOI":"10.1523\/JNEUROSCI.4279-15.2016","article-title":"Shank modulates postsynaptic Wnt signaling to regulate synaptic development","volume":"36","author":"Harris","year":"2016","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.celrep.2020.02.088_bib29","doi-asserted-by":"crossref","first-page":"e4194","DOI":"10.1371\/journal.pone.0004194","article-title":"An essential role of the universal polarity protein, aPKClambda, on the maintenance of podocyte slit diaphragms","volume":"4","author":"Hirose","year":"2009","journal-title":"PLoS ONE"},{"key":"10.1016\/j.celrep.2020.02.088_bib30","doi-asserted-by":"crossref","first-page":"6690","DOI":"10.1128\/MCB.24.15.6690-6700.2004","article-title":"Rap1 regulates the formation of E-cadherin-based cell-cell contacts","volume":"24","author":"Hogan","year":"2004","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib31","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1242\/jcs.043174","article-title":"Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells","volume":"122","author":"Horikoshi","year":"2009","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2020.02.088_bib32","doi-asserted-by":"crossref","first-page":"D261","DOI":"10.1093\/nar\/gkr1122","article-title":"PhosphoSitePlus: a comprehensive resource for investigating the structure and function of experimentally determined post-translational modifications in man and mouse","volume":"40","author":"Hornbeck","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.celrep.2020.02.088_bib33","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1681\/ASN.2008080871","article-title":"Loss of podocyte aPKClambda\/iota causes polarity defects and nephrotic syndrome","volume":"20","author":"Huber","year":"2009","journal-title":"J.\u00a0Am. Soc. Nephrol."},{"key":"10.1016\/j.celrep.2020.02.088_bib34","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1083\/jcb.201104143","article-title":"aPKC phosphorylates JAM-A at Ser285 to promote cell contact maturation and tight junction formation","volume":"196","author":"Iden","year":"2012","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib35","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1083\/jcb.143.1.95","article-title":"An atypical PKC directly associates and colocalizes at the epithelial tight junction with ASIP, a mammalian homologue of Caenorhabditis elegans polarity protein PAR-3","volume":"143","author":"Izumi","year":"1998","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib36","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.neuron.2013.03.016","article-title":"Modeling autism by SHANK gene mutations in mice","volume":"78","author":"Jiang","year":"2013","journal-title":"Neuron"},{"key":"10.1016\/j.celrep.2020.02.088_bib37","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1038\/35019573","article-title":"The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42","volume":"2","author":"Joberty","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib38","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.celrep.2020.02.088_bib39","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1242\/jcs.03306","article-title":"Rap1: a key regulator in cell-cell junction formation","volume":"120","author":"Kooistra","year":"2007","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2020.02.088_bib40","doi-asserted-by":"crossref","first-page":"e1002521","DOI":"10.1371\/journal.pgen.1002521","article-title":"Genetic and functional analyses of SHANK2 mutations suggest a multiple hit model of autism spectrum disorders","volume":"8","author":"Leblond","year":"2012","journal-title":"PLoS Genet."},{"key":"10.1016\/j.celrep.2020.02.088_bib41","doi-asserted-by":"crossref","first-page":"e1004580","DOI":"10.1371\/journal.pgen.1004580","article-title":"Meta-analysis of SHANK mutations in autism spectrum disorders: a gradient of severity in cognitive impairments","volume":"10","author":"Leblond","year":"2014","journal-title":"PLoS Genet."},{"key":"10.1016\/j.celrep.2020.02.088_bib42","doi-asserted-by":"crossref","first-page":"8104","DOI":"10.1074\/jbc.M109.055079","article-title":"BetaPix up-regulates Na+\/H+ exchanger 3 through a Shank2-mediated protein-protein interaction","volume":"285","author":"Lee","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.02.088_bib43","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1038\/ncb3487","article-title":"SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras","volume":"19","author":"Lilja","year":"2017","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib44","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/35019582","article-title":"A mammalian PAR-3-PAR-6 complex implicated in Cdc42\/Rac1 and aPKC signalling and cell polarity","volume":"2","author":"Lin","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib45","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/nrc3169","article-title":"Epithelial cell polarity, stem cells and cancer","volume":"12","author":"Martin-Belmonte","year":"2011","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.celrep.2020.02.088_bib46","doi-asserted-by":"crossref","first-page":"30573","DOI":"10.1074\/jbc.M004578200","article-title":"Characterization of the interaction between protein 4.1R and ZO-2. A possible link between the tight junction and the actin cytoskeleton","volume":"275","author":"Mattagajasingh","year":"2000","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.02.088_bib47","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/nrm1055","article-title":"Signalling to and from tight junctions","volume":"4","author":"Matter","year":"2003","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib48","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1101\/gad.1795909","article-title":"The Par3\/aPKC interaction is essential for end bud remodeling and progenitor differentiation during mammary gland morphogenesis","volume":"23","author":"McCaffrey","year":"2009","journal-title":"Genes Dev."},{"key":"10.1016\/j.celrep.2020.02.088_bib49","doi-asserted-by":"crossref","first-page":"a009654","DOI":"10.1101\/cshperspect.a009654","article-title":"Signaling pathways in cell polarity","volume":"4","author":"McCaffrey","year":"2012","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib50","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1042\/bj20031577","article-title":"Characterization of an ankyrin repeat-containing Shank2 isoform (Shank2E) in liver epithelial cells","volume":"380","author":"McWilliams","year":"2004","journal-title":"Biochem. J."},{"key":"10.1016\/j.celrep.2020.02.088_bib51","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.str.2014.05.015","article-title":"Structures of SMG1-UPFs complexes: SMG1 contributes to regulate UPF2-dependent activation of UPF1 in NMD","volume":"22","author":"Melero","year":"2014","journal-title":"Structure"},{"key":"10.1016\/j.celrep.2020.02.088_bib52","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1126\/science.283.5402.682","article-title":"Transduction of human CD34+ cells that mediate long-term engraftment of NOD\/SCID mice by HIV vectors","volume":"283","author":"Miyoshi","year":"1999","journal-title":"Science"},{"key":"10.1016\/j.celrep.2020.02.088_bib53","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1091\/mbc.e13-06-0298","article-title":"JAM-A associates with ZO-2, afadin, and PDZ-GEF1 to activate Rap2c and regulate epithelial barrier function","volume":"24","author":"Monteiro","year":"2013","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.celrep.2020.02.088_bib54","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1006\/bbrc.2001.5247","article-title":"Possible applications of conditionally immortalized tissue cell lines with differentiation functions","volume":"286","author":"Obinata","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.celrep.2020.02.088_bib55","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/S0955-0674(00)00264-7","article-title":"Intercellular junctions and cellular polarity: the PAR-aPKC complex, a conserved core cassette playing fundamental roles in cell polarity","volume":"13","author":"Ohno","year":"2001","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib56","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1074\/mcp.M200025-MCP200","article-title":"Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics","volume":"1","author":"Ong","year":"2002","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.celrep.2020.02.088_bib57","doi-asserted-by":"crossref","first-page":"2352","DOI":"10.1242\/jcs.03470","article-title":"Cooperative roles of Par-3 and afadin in the formation of adherens and tight junctions","volume":"120","author":"Ooshio","year":"2007","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2020.02.088_bib58","doi-asserted-by":"crossref","first-page":"5003","DOI":"10.1074\/jbc.M109.043760","article-title":"Involvement of the interaction of afadin with ZO-1 in the formation of tight junctions in Madin-Darby canine kidney cells","volume":"285","author":"Ooshio","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.02.088_bib59","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.yexcr.2018.12.008","article-title":"The PDZ-GEF Gef26 regulates synapse development and function via FasII and Rap1 at the Drosophila neuromuscular junction","volume":"374","author":"Ou","year":"2019","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.celrep.2020.02.088_bib60","doi-asserted-by":"crossref","first-page":"100","DOI":"10.4161\/cam.27352","article-title":"Rap1 signaling in endothelial barrier control","volume":"8","author":"Pannekoek","year":"2014","journal-title":"Cell Adhes. Migr."},{"key":"10.1016\/j.celrep.2020.02.088_bib61","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1172\/JCI67846","article-title":"Podocyte-specific RAP1GAP expression contributes to focal segmental glomerulosclerosis-associated glomerular injury","volume":"124","author":"Potla","year":"2014","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.celrep.2020.02.088_bib62","doi-asserted-by":"crossref","first-page":"35127","DOI":"10.1074\/jbc.M404917200","article-title":"Rap1 regulates E-cadherin-mediated cell-cell adhesion","volume":"279","author":"Price","year":"2004","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.02.088_bib63","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1177\/002215540104900511","article-title":"The cortactin-binding postsynaptic density protein proSAP1 in non-neuronal cells","volume":"49","author":"Redecker","year":"2001","journal-title":"J.\u00a0Histochem. Cytochem."},{"key":"10.1016\/j.celrep.2020.02.088_bib64","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1113\/expphysiol.1982.sp002637","article-title":"Isolation and culture of renal cortical tubules from neonate rabbit kidneys","volume":"67","author":"Richardson","year":"1982","journal-title":"Q.\u00a0J. Exp. Physiol."},{"key":"10.1016\/j.celrep.2020.02.088_bib65","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/nrm2847","article-title":"PKC and the control of localized signal dynamics","volume":"11","author":"Rosse","year":"2010","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib66","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/S0896-6273(04)00255-7","article-title":"New synaptic bouton formation is disrupted by misregulation of microtubule stability in aPKC mutants","volume":"42","author":"Ruiz-Canada","year":"2004","journal-title":"Neuron"},{"key":"10.1016\/j.celrep.2020.02.088_bib67","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1083\/jcb.137.2.359","article-title":"The apical submembrane cytoskeleton participates in the organization of the apical pole in epithelial cells","volume":"137","author":"Salas","year":"1997","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib68","doi-asserted-by":"crossref","first-page":"3069","DOI":"10.1128\/MCB.18.5.3069","article-title":"Localization of atypical protein kinase C isoforms into lysosome-targeted endosomes through interaction with p62","volume":"18","author":"Sanchez","year":"1998","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib69","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.1242\/jcs.160879","article-title":"Regulation of epithelial cell polarity by PAR-3 depends on Girdin transcription and Girdin-G\u03b1i3 signaling","volume":"128","author":"Sasaki","year":"2015","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2020.02.088_bib70","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1038\/nn1295","article-title":"The sequential activity of the GTPases Rap1B and Cdc42 determines neuronal polarity","volume":"7","author":"Schwamborn","year":"2004","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.celrep.2020.02.088_bib71","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1242\/jcs.113.11.1851","article-title":"The Shank family of scaffold proteins","volume":"113","author":"Sheng","year":"2000","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2020.02.088_bib72","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1146\/annurev.cellbio.22.010305.104219","article-title":"Tight junctions and cell polarity","volume":"22","author":"Shin","year":"2006","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib73","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1097\/MNH.0b013e32832e316d","article-title":"Podocyte polarity signalling","volume":"18","author":"Simons","year":"2009","journal-title":"Curr. Opin. Nephrol. Hypertens."},{"key":"10.1016\/j.celrep.2020.02.088_bib74","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.ceb.2011.07.005","article-title":"Epithelial polarity and morphogenesis","volume":"23","author":"St Johnston","year":"2011","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib75","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1007\/s00018-017-2729-0","article-title":"Junctional adhesion molecule-A: functional diversity through molecular promiscuity","volume":"75","author":"Steinbacher","year":"2018","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.celrep.2020.02.088_bib76","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1242\/jcs.02898","article-title":"The PAR-aPKC system: lessons in polarity","volume":"119","author":"Suzuki","year":"2006","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2020.02.088_bib77","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1083\/jcb.152.6.1183","article-title":"Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures","volume":"152","author":"Suzuki","year":"2001","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib78","doi-asserted-by":"crossref","first-page":"3565","DOI":"10.1242\/jcs.00032","article-title":"aPKC kinase activity is required for the asymmetric differentiation of the premature junctional complex during epithelial cell polarization","volume":"115","author":"Suzuki","year":"2002","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2020.02.088_bib79","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1016\/j.cub.2004.08.021","article-title":"aPKC acts upstream of PAR-1b in both the establishment and maintenance of mammalian epithelial polarity","volume":"14","author":"Suzuki","year":"2004","journal-title":"Curr. Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib80","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1002\/jcp.1041050220","article-title":"Growth of functional primary cultures of kidney epithelial cells in defined medium","volume":"105","author":"Taub","year":"1980","journal-title":"J.\u00a0Cell. Physiol."},{"key":"10.1016\/j.celrep.2020.02.088_bib81","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1056\/NEJMra052131","article-title":"Hereditary proteinuria syndromes and mechanisms of proteinuria","volume":"354","author":"Tryggvason","year":"2006","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.celrep.2020.02.088_bib82","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1083\/jcb.104.5.1249","article-title":"Modulation of the expression of an apical plasma membrane protein of Madin-Darby canine kidney epithelial cells: cell-cell interactions control the appearance of a novel intracellular storage compartment","volume":"104","author":"Vega-Salas","year":"1987","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib83","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1083\/jcb.107.5.1717","article-title":"Exocytosis of vacuolar apical compartment (VAC): a cell-cell contact controlled mechanism for the establishment of the apical plasma membrane domain in epithelial cells","volume":"107","author":"Vega-Salas","year":"1988","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib84","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.str.2012.02.022","article-title":"Substrate recognition mechanism of atypical protein kinase Cs revealed by the structure of PKC\u03b9 in complex with a substrate peptide from Par-3","volume":"20","author":"Wang","year":"2012","journal-title":"Structure"},{"key":"10.1016\/j.celrep.2020.02.088_bib85","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1083\/jcb.150.6.1361","article-title":"Drosophila atypical protein kinase C associates with Bazooka and controls polarity of epithelia and neuroblasts","volume":"150","author":"Wodarz","year":"2000","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib86","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/j.neuron.2005.09.027","article-title":"Activity-dependent dendritic spine structural plasticity is regulated by small GTPase Rap1 and its target AF-6","volume":"48","author":"Xie","year":"2005","journal-title":"Neuron"},{"key":"10.1016\/j.celrep.2020.02.088_bib87","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1083\/jcb.139.3.785","article-title":"The Ras target AF-6 interacts with ZO-1 and serves as a peripheral component of tight junctions in epithelial cells","volume":"139","author":"Yamamoto","year":"1997","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib88","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1046\/j.1365-2443.2001.00453.x","article-title":"PAR-6 regulates aPKC activity in a novel way and mediates cell-cell contact-induced formation of the epithelial junctional complex","volume":"6","author":"Yamanaka","year":"2001","journal-title":"Genes Cells"},{"key":"10.1016\/j.celrep.2020.02.088_bib89","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/S0960-9822(03)00244-6","article-title":"Mammalian Lgl forms a protein complex with PAR-6 and aPKC independently of PAR-3 to regulate epithelial cell polarity","volume":"13","author":"Yamanaka","year":"2003","journal-title":"Curr. Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib90","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.cub.2011.03.029","article-title":"KIBRA suppresses apical exocytosis through inhibition of aPKC kinase activity in epithelial cells","volume":"21","author":"Yoshihama","year":"2011","journal-title":"Curr. Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib91","doi-asserted-by":"crossref","first-page":"F31","DOI":"10.1083\/jcb.151.5.F31","article-title":"Tight junction, a platform for trafficking and signaling protein complexes","volume":"151","author":"Zahraoui","year":"2000","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2020.02.088_bib92","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.devcel.2007.11.020","article-title":"The PAR-6 polarity protein regulates dendritic spine morphogenesis through p190 RhoGAP and the Rho GTPase","volume":"14","author":"Zhang","year":"2008","journal-title":"Dev. Cell"},{"key":"10.1016\/j.celrep.2020.02.088_bib93","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1016\/S0896-6273(00)80409-2","article-title":"Enlightening the postsynaptic density","volume":"19","author":"Ziff","year":"1997","journal-title":"Neuron"}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S221112472030262X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S221112472030262X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T10:53:27Z","timestamp":1761908007000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S221112472030262X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4]]},"references-count":93,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["S221112472030262X"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2020.02.088","relation":{},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2020,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Shank2 Binds to aPKC and Controls Tight Junction Formation with Rap1 Signaling during Establishment of Epithelial Cell Polarity","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2020.02.088","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors.","name":"copyright","label":"Copyright"}],"article-number":"107407"}}