{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:19:46Z","timestamp":1792603186317,"version":"4.5.0"},"reference-count":75,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T00:00:00Z","timestamp":1593043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2020,7]]},"DOI":"10.1016\/j.celrep.2020.107909","type":"journal-article","created":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T10:38:33Z","timestamp":1595327913000},"page":"107909","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":68,"title":["Structural Insights into the SPRED1-Neurofibromin-KRAS Complex and Disruption of SPRED1-Neurofibromin Interaction by Oncogenic EGFR"],"prefix":"10.1016","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3892-7162","authenticated-orcid":false,"given":"Wupeng","family":"Yan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan","family":"Markegard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7630-3962","authenticated-orcid":false,"given":"Srisathiyanarayanan","family":"Dharmaiah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8364-5303","authenticated-orcid":false,"given":"Anatoly","family":"Urisman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3954-6671","authenticated-orcid":false,"given":"Matthew","family":"Drew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9987-1687","authenticated-orcid":false,"given":"Dominic","family":"Esposito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Scheffzek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7523-116X","authenticated-orcid":false,"given":"Dwight V.","family":"Nissley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"McCormick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9717-4618","authenticated-orcid":false,"given":"Dhirendra K.","family":"Simanshu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2020.107909_bib1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.celrep.2020.107909_bib2","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/j.bpj.2019.02.004","article-title":"KRAS Prenylation Is Required for Bivalent Binding with Calmodulin in a Nucleotide-Independent Manner","volume":"116","author":"Agamasu","year":"2019","journal-title":"Biophys. J."},{"key":"10.1016\/j.celrep.2020.107909_bib3","doi-asserted-by":"crossref","first-page":"16409","DOI":"10.1074\/jbc.271.27.16409","article-title":"Structural differences in the minimal catalytic domains of the GTPase-activating proteins p120GAP and neurofibromin","volume":"271","author":"Ahmadian","year":"1996","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.107909_bib4","doi-asserted-by":"crossref","first-page":"4535","DOI":"10.1021\/bi962556y","article-title":"Individual rate constants for the interaction of Ras proteins with GTPase-activating proteins determined by fluorescence spectroscopy","volume":"36","author":"Ahmadian","year":"1997","journal-title":"Biochemistry"},{"key":"10.1016\/j.celrep.2020.107909_bib5","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1038\/nsb0997-686","article-title":"Confirmation of the arginine-finger hypothesis for the GAP-stimulated GTP-hydrolysis reaction of Ras","volume":"4","author":"Ahmadian","year":"1997","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.celrep.2020.107909_bib6","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/jhg.2015.114","article-title":"Recent advances in RASopathies","volume":"61","author":"Aoki","year":"2016","journal-title":"J.\u00a0Hum. Genet."},{"key":"10.1016\/j.celrep.2020.107909_bib7","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1093\/nar\/28.1.45","article-title":"The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000","volume":"28","author":"Bairoch","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.celrep.2020.107909_bib8","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1074\/mcp.O113.037200","article-title":"MS-viewer: a web-based spectral viewer for proteomics results","volume":"13","author":"Baker","year":"2014","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.celrep.2020.107909_bib9","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0014-5793(01)03291-4","article-title":"EVH1 domains: structure, function and interactions","volume":"513","author":"Ball","year":"2002","journal-title":"FEBS Lett."},{"key":"10.1016\/j.celrep.2020.107909_bib10","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/0092-8674(90)90151-4","article-title":"The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins","volume":"63","author":"Ballester","year":"1990","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.107909_bib11","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1042\/BSR20110060","article-title":"Alternative splicing of the neurofibromatosis type I pre-mRNA","volume":"32","author":"Barron","year":"2012","journal-title":"Biosci. Rep."},{"key":"10.1016\/j.celrep.2020.107909_bib12","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0896-6273(00)81145-9","article-title":"Structure of the Homer EVH1 domain-peptide complex reveals a new twist in polyproline recognition","volume":"26","author":"Beneken","year":"2000","journal-title":"Neuron"},{"key":"10.1016\/j.celrep.2020.107909_bib13","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1002\/1097-0142(19860315)57:6<1225::AID-CNCR2820570627>3.0.CO;2-P","article-title":"Neurofibromatosis and childhood tumors","volume":"57","author":"Blatt","year":"1986","journal-title":"Cancer"},{"key":"10.1016\/j.celrep.2020.107909_bib14","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/nbt.4062","article-title":"Dual gene activation and knockout screen reveals directional dependencies in genetic networks","volume":"36","author":"Boettcher","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.celrep.2020.107909_bib15","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.cell.2007.05.018","article-title":"GEFs and GAPs: critical elements in the control of small G proteins","volume":"129","author":"Bos","year":"2007","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.107909_bib16","doi-asserted-by":"crossref","first-page":"107","DOI":"10.2302\/kjm.2013-0002-RE","article-title":"Legius syndrome, an Update. Molecular pathology of mutations in SPRED1","volume":"62","author":"Brems","year":"2013","journal-title":"Keio J. Med."},{"key":"10.1016\/j.celrep.2020.107909_bib17","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1002\/humu.22152","article-title":"Review and update of SPRED1 mutations causing Legius syndrome","volume":"33","author":"Brems","year":"2012","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.celrep.2020.107909_bib18","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1038\/nature07385","article-title":"Comprehensive genomic characterization defines human glioblastoma genes and core pathways","volume":"455","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2020.107909_bib19","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1074\/mcp.D500001-MCP200","volume":"4","author":"Chalkley","year":"2005","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.celrep.2020.107909_bib20","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1152\/physrev.00003.2012","article-title":"Regulation of small GTPases by GEFs, GAPs, and GDIs","volume":"93","author":"Cherfils","year":"2013","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.celrep.2020.107909_bib21","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nature13385","article-title":"Comprehensive molecular profiling of lung adenocarcinoma","volume":"511","author":"Collisson","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2020.107909_bib22","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/0092-8674(92)90407-4","article-title":"Abnormal regulation of mammalian p21ras contributes to malignant tumor growth in von Recklinghausen (type 1) neurofibromatosis","volume":"69","author":"DeClue","year":"1992","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.107909_bib23","doi-asserted-by":"crossref","first-page":"E1985","DOI":"10.1002\/humu.21404","article-title":"Legius syndrome in fourteen families","volume":"32","author":"Denayer","year":"2011","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.celrep.2020.107909_bib24","doi-asserted-by":"crossref","first-page":"10512","DOI":"10.1038\/s41598-019-46846-w","article-title":"Structures of N-terminally processed KRAS provide insight into the role of N-acetylation","volume":"9","author":"Dharmaiah","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.celrep.2020.107909_bib25","first-page":"23","article-title":"GTPase activating proteins: critical regulators of intracellular signaling","volume":"1602","author":"Donovan","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.celrep.2020.107909_bib26","doi-asserted-by":"crossref","first-page":"7497","DOI":"10.1073\/pnas.1607298113","article-title":"The neurofibromin recruitment factor Spred1 binds to the GAP related domain without affecting Ras inactivation","volume":"113","author":"Dunzendorfer-Matt","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2020.107909_bib27","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.celrep.2020.107909_bib28","doi-asserted-by":"crossref","first-page":"3889","DOI":"10.1016\/j.jmb.2019.07.038","article-title":"Pathogenic Mutations Associated with Legius Syndrome Modify the Spred1 Surface and Are Involved in Direct Binding to the Ras Inactivator Neurofibromin","volume":"431","author":"F\u00fchrer","year":"2019","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.celrep.2020.107909_bib29","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1186\/2047-783X-14-3-102","article-title":"Neurofibromatosis","volume":"14","author":"Gerber","year":"2009","journal-title":"Eur. J. Med. Res."},{"key":"10.1016\/j.celrep.2020.107909_bib30","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S0092-8674(00)81341-0","article-title":"Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics","volume":"87","author":"Gertler","year":"1996","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.107909_bib31","first-page":"2050","article-title":"Mutational and kinetic analyses of the GTPase-activating protein (GAP)-p21 interaction: the C-terminal domain of GAP is not sufficient for full activity","volume":"12","author":"Gideon","year":"1992","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.celrep.2020.107909_bib32","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.febslet.2004.11.114","article-title":"1.15 A crystal structure of the X. tropicalis Spred1 EVH1 domain suggests a fourth distinct peptide-binding mechanism within the EVH1 family","volume":"579","author":"Harmer","year":"2005","journal-title":"FEBS Lett."},{"key":"10.1016\/j.celrep.2020.107909_bib33","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1128\/MCB.00019-14","article-title":"Neurofibromatosis type 1 alternative splicing is a key regulator of Ras signaling in neurons","volume":"34","author":"Hinman","year":"2014","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.celrep.2020.107909_bib34","doi-asserted-by":"crossref","first-page":"3124","DOI":"10.1074\/jbc.M115.703710","article-title":"Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1","volume":"291","author":"Hirata","year":"2016","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.107909_bib35","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1242\/jcs.182873","article-title":"RAS isoforms and mutations in cancer at a glance","volume":"129","author":"Hobbs","year":"2016","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2020.107909_bib36","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/pro.3280","article-title":"Improvements to the APBS biomolecular solvation software suite","volume":"27","author":"Jurrus","year":"2018","journal-title":"Protein Sci."},{"key":"10.1016\/j.celrep.2020.107909_bib37","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1107\/S0907444909047374","article-title":"Integration, scaling, space-group assignment and post-refinement","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.celrep.2020.107909_bib38","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/0092-8674(90)90220-9","article-title":"PDGF beta-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex","volume":"61","author":"Kaplan","year":"1990","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.107909_bib39","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/S0006-291X(03)00259-6","article-title":"Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation","volume":"302","author":"Kato","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.celrep.2020.107909_bib40","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1634\/theoncologist.5-6-477","article-title":"Malignancy in neurofibromatosis type 1","volume":"5","author":"Korf","year":"2000","journal-title":"Oncologist"},{"key":"10.1016\/j.celrep.2020.107909_bib41","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1055\/s-0036-1579766","article-title":"Neurofibromatosis: A Review of NF1, NF2, and Schwannomatosis","volume":"5","author":"Kresak","year":"2016","journal-title":"J.\u00a0Pediatr. Genet."},{"key":"10.1016\/j.celrep.2020.107909_bib42","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0092-8674(92)90408-5","article-title":"Somatic mutations in the neurofibromatosis 1 gene in human tumors","volume":"69","author":"Li","year":"1992","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.107909_bib43","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1093\/bioinformatics\/btq054","article-title":"Skyline: an open source document editor for creating and analyzing targeted proteomics experiments","volume":"26","author":"MacLean","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.celrep.2020.107909_bib44","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/0092-8674(90)90150-D","article-title":"The GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21","volume":"63","author":"Martin","year":"1990","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.107909_bib45","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1101\/gad.197434.112","article-title":"SPRED proteins provide a NF-ty link to Ras suppression","volume":"26","author":"McClatchey","year":"2012","journal-title":"Genes Dev."},{"key":"10.1016\/j.celrep.2020.107909_bib46","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"J.\u00a0Appl. Cryst."},{"key":"10.1016\/j.celrep.2020.107909_bib47","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1001\/jama.2009.1663","article-title":"Clinical and mutational spectrum of neurofibromatosis type 1-like syndrome","volume":"302","author":"Messiaen","year":"2009","journal-title":"JAMA"},{"key":"10.1016\/j.celrep.2020.107909_bib48","doi-asserted-by":"crossref","first-page":"e01456","DOI":"10.7554\/eLife.01456","article-title":"Collaboration gets the most out of software","volume":"2","author":"Morin","year":"2013","journal-title":"eLife"},{"key":"10.1016\/j.celrep.2020.107909_bib49","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1111\/j.1365-2443.2005.00886.x","article-title":"The Sprouty-related protein, Spred-1, localizes in a lipid raft\/caveola and inhibits ERK activation in collaboration with caveolin-1","volume":"10","author":"Nonami","year":"2005","journal-title":"Genes Cells"},{"key":"10.1016\/j.celrep.2020.107909_bib50","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1038\/embor.2008.20","article-title":"The C2 domain of SynGAP is essential for stimulation of the Rap GTPase reaction","volume":"9","author":"Pena","year":"2008","journal-title":"EMBO Rep."},{"key":"10.1016\/j.celrep.2020.107909_bib51","doi-asserted-by":"crossref","first-page":"833","DOI":"10.2741\/3281","article-title":"Diversity of polyproline recognition by EVH1 domains","volume":"14","author":"Peterson","year":"2009","journal-title":"Front. Biosci."},{"key":"10.1016\/j.celrep.2020.107909_bib52","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s40246-017-0109-3","article-title":"The NF1 somatic mutational landscape in sporadic human cancers","volume":"11","author":"Philpott","year":"2017","journal-title":"Hum. Genomics"},{"key":"10.1016\/j.celrep.2020.107909_bib53","doi-asserted-by":"crossref","first-page":"22122","DOI":"10.1073\/pnas.1908353116","article-title":"KRAS G13D sensitivity to neurofibromin-mediated GTP hydrolysis","volume":"116","author":"Rabara","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2020.107909_bib54","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nrc3911","article-title":"A RASopathy gene commonly mutated in cancer: the neurofibromatosis type 1 tumour suppressor","volume":"15","author":"Ratner","year":"2015","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.celrep.2020.107909_bib55","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1002\/j.1460-2075.1995.tb07146.x","article-title":"The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins","volume":"14","author":"Reinhard","year":"1995","journal-title":"EMBO J."},{"key":"10.1016\/j.celrep.2020.107909_bib56","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/41805","article-title":"Crystal structure of a small G protein in complex with the GTPase-activating protein rhoGAP","volume":"388","author":"Rittinger","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2020.107909_bib57","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1038\/39651","article-title":"Structure at 1.65 A of RhoA and its GTPase-activating protein in complex with a transition-state analogue","volume":"389","author":"Rittinger","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2020.107909_bib58","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1126\/science.277.5324.333","article-title":"The Ras-RasGAP complex: structural basis for GTPase activation and its loss in oncogenic Ras mutants","volume":"277","author":"Scheffzek","year":"1997","journal-title":"Science"},{"key":"10.1016\/j.celrep.2020.107909_bib59","doi-asserted-by":"crossref","first-page":"4313","DOI":"10.1093\/emboj\/17.15.4313","article-title":"Structural analysis of the GAP-related domain from neurofibromin and its implications","volume":"17","author":"Scheffzek","year":"1998","journal-title":"EMBO J."},{"key":"10.1016\/j.celrep.2020.107909_bib60","doi-asserted-by":"crossref","first-page":"9480","DOI":"10.1074\/jbc.273.16.9480","article-title":"The importance of two conserved arginine residues for catalysis by the ras GTPase-activating protein, neurofibromin","volume":"273","author":"Sermon","year":"1998","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.107909_bib61","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1016\/j.celrep.2014.07.036","article-title":"Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser\/Thr-based signaling","volume":"8","author":"Sharma","year":"2014","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2020.107909_bib62","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/S0021-9258(17)49919-4","article-title":"Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer","volume":"295","author":"Sherekar","year":"2020","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2020.107909_bib63","doi-asserted-by":"crossref","first-page":"2612","DOI":"10.1128\/MCB.00191-16","article-title":"SPRED1 Interferes with K-ras but Not H-ras Membrane Anchorage and Signaling","volume":"36","author":"Siljam\u00e4ki","year":"2016","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.celrep.2020.107909_bib64","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.cell.2017.06.009","article-title":"RAS Proteins and Their Regulators in Human Disease","volume":"170","author":"Simanshu","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.107909_bib65","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1101\/gad.190876.112","article-title":"A shared molecular mechanism underlies the human rasopathies Legius syndrome and Neurofibromatosis-1","volume":"26","author":"Stowe","year":"2012","journal-title":"Genes Dev."},{"key":"10.1016\/j.celrep.2020.107909_bib66","series-title":"National Institutes of Health Consensus Development Conference Statement: neurofibromatosis. Bethesda, Md., USA","first-page":"172","volume":"1","author":"Stumpf","year":"1988"},{"key":"10.1016\/j.celrep.2020.107909_bib67","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1534\/g3.111.000687","article-title":"The SPRED1 Variants Repository for Legius Syndrome","volume":"1","author":"Sumner","year":"2011","journal-title":"G3 (Bethesda)"},{"key":"10.1016\/j.celrep.2020.107909_bib68","doi-asserted-by":"crossref","first-page":"79","DOI":"10.3390\/genes10020079","article-title":"Genome Assembly and Annotation of the Trichoplusia ni Tni-FNL Insect Cell Line Enabled by Long-Read Technologies","volume":"10","author":"Talsania","year":"2019","journal-title":"Genes (Basel)"},{"key":"10.1016\/j.celrep.2020.107909_bib69","doi-asserted-by":"crossref","first-page":"4541","DOI":"10.1128\/MCB.01600-06","article-title":"Spreds are essential for embryonic lymphangiogenesis by regulating vascular endothelial growth factor receptor 3 signaling","volume":"27","author":"Taniguchi","year":"2007","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.celrep.2020.107909_bib70","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/978-1-4939-6887-9_5","article-title":"Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters","volume":"1586","author":"Taylor","year":"2017","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.celrep.2020.107909_bib71","doi-asserted-by":"crossref","first-page":"937","DOI":"10.2741\/3727","article-title":"Genetic basis of tumorigenesis in NF1 malignant peripheral nerve sheath tumors","volume":"16","author":"Upadhyaya","year":"2011","journal-title":"Front. Biosci."},{"key":"10.1016\/j.celrep.2020.107909_bib72","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1038\/35088082","article-title":"Spred is a Sprouty-related suppressor of Ras signalling","volume":"412","author":"Wakioka","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2020.107909_bib73","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","article-title":"Overview of the CCP4 suite and current developments","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.celrep.2020.107909_bib74","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/0092-8674(90)90024-9","article-title":"The neurofibromatosis type 1 gene encodes a protein related to GAP","volume":"62","author":"Xu","year":"1990","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.107909_bib75","doi-asserted-by":"crossref","first-page":"5873","DOI":"10.18632\/oncotarget.2194","article-title":"The NF1 gene revisited - from bench to bedside","volume":"5","author":"Yap","year":"2014","journal-title":"Oncotarget"}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124720308901?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124720308901?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T11:03:22Z","timestamp":1761908602000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124720308901"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7]]},"references-count":75,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["S2211124720308901"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2020.107909","relation":{},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2020,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural Insights into the SPRED1-Neurofibromin-KRAS Complex and Disruption of SPRED1-Neurofibromin Interaction by Oncogenic EGFR","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2020.107909","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Author(s).","name":"copyright","label":"Copyright"}],"article-number":"107909"}}