{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:18:45Z","timestamp":1790849925898,"version":"4.1.0"},"reference-count":60,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:00:00Z","timestamp":1602028800000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006975","name":"Indiana Clinical and Translational Sciences Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006975","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["KL2TR001882"],"award-info":[{"award-number":["KL2TR001882"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["5P30AG028748 \/ UL1TR000124"],"award-info":[{"award-number":["5P30AG028748 \/ UL1TR000124"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI119927"],"award-info":[{"award-number":["R21AI119927"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1AG049753"],"award-info":[{"award-number":["RO1AG049753"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016206","name":"Clinical and Translational Science Institute, University of California, Los Angeles","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016206","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008623","name":"Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008623","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007185","name":"University of California, Los Angeles","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007185","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2020,10]]},"DOI":"10.1016\/j.celrep.2020.108320","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T10:37:25Z","timestamp":1603795045000},"page":"108320","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":44,"title":["In\u00a0Vitro Recapitulation of Murine Thymopoiesis from Single Hematopoietic Stem Cells"],"prefix":"10.1016","volume":"33","author":[{"given":"Am\u00e9lie","family":"Montel-Hagen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7347-3330","authenticated-orcid":false,"given":"David","family":"Casero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Zampieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4837-6624","authenticated-orcid":false,"given":"Nicholas","family":"Jackson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suwen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0193-1654","authenticated-orcid":false,"given":"Brent","family":"Chick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongbin","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie C.","family":"de Barros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher S.","family":"Seet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gay M.","family":"Crooks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2020.108320_bib1","doi-asserted-by":"crossref","first-page":"739","DOI":"10.4049\/jimmunol.147.3.739","article-title":"Characterization of a new minor lymphocyte stimulatory system. I. Cluster of self antigens recognized by \u201cI-E-reactive\u201d V beta s, V beta 5, V beta 11, and V beta 12 T\u00a0cell receptors for antigen","volume":"147","author":"Abe","year":"1991","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib2","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.4049\/jimmunol.1801373","article-title":"T cell acute lymphoblastic leukemia as a consequence of thymus autonomy","volume":"202","author":"Ballesteros-Arias","year":"2019","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib3","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1084\/jem.187.11.1839","article-title":"The role of the thymus and recent thymic migrants in the maintenance of the adult peripheral lymphocyte pool","volume":"187","author":"Berzins","year":"1998","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2020.108320_bib4","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1084\/jem.169.4.1405","article-title":"The MHC molecule I-E is necessary but not sufficient for the clonal deletion of V beta 11-bearing T\u00a0cells","volume":"169","author":"Bill","year":"1989","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2020.108320_bib5","first-page":"269","article-title":"Class I and class II MHC gene products differentially affect the fate of V beta 5 bearing thymocytes","volume":"4","author":"Bill","year":"1990","journal-title":"J.\u00a0Mol. Cell. Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib6","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1084\/jem.20121412","article-title":"Self-renewal of thymocytes in the absence of competitive precursor replenishment","volume":"209","author":"Boehm","year":"2012","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2020.108320_bib7","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.1084\/jem.180.6.2401","article-title":"Entry of naive CD4 T\u00a0cells into peripheral lymph nodes requires L-selectin","volume":"180","author":"Bradley","year":"1994","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2020.108320_bib8","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.autrev.2018.02.001","article-title":"TCR\u03b1\u03b2+CD3+CD4-CD8- (double negative) T\u00a0cells in autoimmunity","volume":"17","author":"Brandt","year":"2018","journal-title":"Autoimmun. Rev."},{"key":"10.1016\/j.celrep.2020.108320_bib9","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.semcdb.2017.07.045","article-title":"Directing T\u00a0cell fate: how thymic antigen presenting cells coordinate thymocyte selection","volume":"84","author":"Breed","year":"2018","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.celrep.2020.108320_bib10","doi-asserted-by":"crossref","first-page":"3120","DOI":"10.4049\/jimmunol.138.10.3120","article-title":"Distinction of virgin and memory T lymphocytes. Stable acquisition of the Pgp-1 glycoprotein concomitant with antigenic stimulation","volume":"138","author":"Budd","year":"1987","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib11","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1038\/ni.3299","article-title":"Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T\u00a0cell lineages","volume":"16","author":"Casero","year":"2015","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib12","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1002\/stem.1731","article-title":"Engineering the human thymic microenvironment to support thymopoiesis in\u00a0vivo","volume":"32","author":"Chung","year":"2014","journal-title":"Stem Cells"},{"key":"10.1016\/j.celrep.2020.108320_bib13","doi-asserted-by":"crossref","first-page":"2144","DOI":"10.1182\/blood-2012-08-447417","article-title":"Intrathymic progenitor cell transplantation across histocompatibility barriers results in the persistence of early thymic progenitors and T-cell differentiation","volume":"121","author":"de Barros","year":"2013","journal-title":"Blood"},{"key":"10.1016\/j.celrep.2020.108320_bib14","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.celrep.2020.108320_bib15","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1038\/mt.2015.77","article-title":"Bioengineering thymus organoids to restore thymic function and induce donor-specific immune tolerance to allografts","volume":"23","author":"Fan","year":"2015","journal-title":"Mol. Ther."},{"key":"10.1016\/j.celrep.2020.108320_bib16","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.immuni.2005.01.016","article-title":"Regulatory T\u00a0cell lineage specification by the forkhead transcription factor foxp3","volume":"22","author":"Fontenot","year":"2005","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2020.108320_bib17","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1084\/jem.170.6.1947","article-title":"Capacity of unprimed CD4+ and CD8+ T\u00a0cells expressing V beta 11 receptors to respond to I-E alloantigens in\u00a0vivo","volume":"170","author":"Gao","year":"1989","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2020.108320_bib18","doi-asserted-by":"crossref","first-page":"4244","DOI":"10.4049\/jimmunol.150.10.4244","article-title":"A developmental pathway involving four phenotypically and functionally distinct subsets of CD3-CD4-CD8- triple-negative adult mouse thymocytes defined by CD44 and CD25 expression","volume":"150","author":"Godfrey","year":"1993","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib19","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1006\/smim.1998.0151","article-title":"In\u00a0vitro models of T\u00a0cell development","volume":"11","author":"Hare","year":"1999","journal-title":"Semin. Immunol."},{"issue":"Appendix","key":"10.1016\/j.celrep.2020.108320_bib20","first-page":"1F","article-title":"Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses","volume":"1","author":"Hodes","year":"2001","journal-title":"Curr Protoc Immunol Appendix"},{"key":"10.1016\/j.celrep.2020.108320_bib21","doi-asserted-by":"crossref","first-page":"2507","DOI":"10.1084\/jem.20080134","article-title":"Delta-like 4 is indispensable in thymic environment specific for T\u00a0cell development","volume":"205","author":"Hozumi","year":"2008","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2020.108320_bib22","first-page":"145","article-title":"Reproducible establishment of hemopoietic supportive stromal cell lines from murine bone marrow","volume":"17","author":"Itoh","year":"1989","journal-title":"Exp. Hematol."},{"key":"10.1016\/j.celrep.2020.108320_bib23","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1084\/jem.20080829","article-title":"Delta-like 4 is the essential, nonredundant ligand for Notch1 during thymic T\u00a0cell lineage commitment","volume":"205","author":"Koch","year":"2008","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2020.108320_bib24","doi-asserted-by":"crossref","first-page":"525","DOI":"10.4049\/jimmunol.155.2.525","article-title":"Leukocyte interactions with vascular endothelium. New insights into selectin-mediated attachment and rolling","volume":"155","author":"Ley","year":"1995","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib25","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.immuni.2007.11.021","article-title":"A two-step process for thymic regulatory T\u00a0cell development","volume":"28","author":"Lio","year":"2008","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2020.108320_bib26","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1038\/nri2989","article-title":"Signal integration and crosstalk during thymocyte migration and emigration","volume":"11","author":"Love","year":"2011","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib27","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.celrep.2020.108320_bib28","doi-asserted-by":"crossref","first-page":"5525","DOI":"10.4049\/jimmunol.1402144","article-title":"Differential requirement for IL-2 and IL-15 during bifurcated development of thymic regulatory T\u00a0cells","volume":"193","author":"Marshall","year":"2014","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib29","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1084\/jem.20120846","article-title":"Thymus-autonomous T\u00a0cell development in the absence of progenitor import","volume":"209","author":"Martins","year":"2012","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2020.108320_bib30","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/nature13317","article-title":"Cell competition is a tumour suppressor mechanism in the thymus","volume":"509","author":"Martins","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2020.108320_bib31","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1038\/ni.2590","article-title":"The transcriptional landscape of \u03b1\u03b2 T\u00a0cell differentiation","volume":"14","author":"Mingueneau","year":"2013","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib32","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/0092-8674(92)90030-G","article-title":"RAG-1-deficient mice have no mature B and T lymphocytes","volume":"68","author":"Mombaerts","year":"1992","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2020.108320_bib33","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.stem.2018.12.011","article-title":"Organoid-induced differentiation of conventional T\u00a0cells from human pluripotent stem cells","volume":"24","author":"Montel-Hagen","year":"2019","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.celrep.2020.108320_bib34","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1471-213X-13-15","article-title":"A bright single-cell resolution live imaging reporter of Notch signaling in the mouse","volume":"13","author":"Nowotschin","year":"2013","journal-title":"BMC Dev. Biol."},{"key":"10.1016\/j.celrep.2020.108320_bib35","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1084\/jem.20120845","article-title":"Thymocytes may persist and differentiate without any input from bone marrow progenitors","volume":"209","author":"Peaudecerf","year":"2012","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2020.108320_bib36","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1152\/physiolgenomics.00185.2007","article-title":"Genetic influence on immune phenotype revealed strain-specific variations in peripheral blood lineages","volume":"34","author":"Petkova","year":"2008","journal-title":"Physiol. Genomics"},{"key":"10.1016\/j.celrep.2020.108320_bib37","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1182\/blood.V84.5.1587.1587","article-title":"Human CD34+ fetal liver stem cells differentiate to T\u00a0cells in a mouse thymic microenvironment","volume":"84","author":"Plum","year":"1994","journal-title":"Blood"},{"key":"10.1016\/j.celrep.2020.108320_bib38","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1038\/77288","article-title":"Efficient generation of human T\u00a0cells from a tissue-engineered thymic organoid","volume":"18","author":"Poznansky","year":"2000","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.celrep.2020.108320_bib39","first-page":"322","article-title":"Generation of T-cell function in organ culture of foetal mouse thymus. II. Mixed lymphocyte culture reactivity","volume":"27","author":"Robinson","year":"1977","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib40","doi-asserted-by":"crossref","first-page":"6649","DOI":"10.4049\/jimmunol.1003703","article-title":"T cell lineage commitment: identity and renunciation","volume":"186","author":"Rothenberg","year":"2011","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib41","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1101\/gad.327163.119","article-title":"Programming for T-lymphocyte fates: modularity and mechanisms","volume":"33","author":"Rothenberg","year":"2019","journal-title":"Genes Dev."},{"key":"10.1016\/j.celrep.2020.108320_bib42","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/nri2232","article-title":"Launching the T-cell-lineage developmental programme","volume":"8","author":"Rothenberg","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib43","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/S1074-7613(02)00474-0","article-title":"Induction of T\u00a0cell development from hematopoietic progenitor cells by delta-like-1 in\u00a0vitro","volume":"17","author":"Schmitt","year":"2002","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2020.108320_bib44","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/nmeth.4237","article-title":"Generation of mature T\u00a0cells from human hematopoietic stem and progenitor cells in artificial thymic organoids","volume":"14","author":"Seet","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.celrep.2020.108320_bib60","first-page":"2498","article-title":"Cytoscape: A software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome"},{"key":"10.1016\/j.celrep.2020.108320_bib45","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1093\/bioinformatics\/btt632","article-title":"Model-based clustering for RNA-seq data","volume":"30","author":"Si","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.celrep.2020.108320_bib46","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.celrep.2020.108320_bib47","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1093\/intimm\/2.4.343","article-title":"Autoimmune thyroiditis induced in mice depleted of particular T\u00a0cell subsets. III. Analysis of regulatory cells suppressing the induction of thyroiditis","volume":"2","author":"Sugihara","year":"1990","journal-title":"Int. Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib48","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.immuni.2005.11.012","article-title":"Developmental and molecular characterization of emerging beta- and gammadelta-selected pre-T cells in the adult mouse thymus","volume":"24","author":"Taghon","year":"2006","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2020.108320_bib49","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1016\/j.immuni.2013.02.022","article-title":"Foxp3 transcription factor is proapoptotic and lethal to developing regulatory T\u00a0cells unless counterbalanced by cytokine survival signals","volume":"38","author":"Tai","year":"2013","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2020.108320_bib50","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1038\/nri1781","article-title":"Journey through the thymus: stromal guides for T-cell development and selection","volume":"6","author":"Takahama","year":"2006","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib51","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1093\/intimm\/dxq020","article-title":"CD28 expression redefines thymocyte development during the pre-T to DP transition","volume":"22","author":"Teague","year":"2010","journal-title":"Int. Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib52","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1600-065X.1993.tb01534.x","article-title":"Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection","volume":"131","author":"Tomonari","year":"1993","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.celrep.2020.108320_bib53","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1084\/jem.170.4.1335","article-title":"Selective decreases in T\u00a0cell receptor V beta expression. Decreased expression of specific V beta families is associated with expression of multiple MHC and non-MHC gene products","volume":"170","author":"Vacchio","year":"1989","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2020.108320_bib54","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1093\/intimm\/11.10.1641","article-title":"Unexpectedly late expression of intracellular CD3epsilon and TCR gammadelta proteins during adult thymus development","volume":"11","author":"Wilson","year":"1999","journal-title":"Int. Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib55","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1126\/science.1968289","article-title":"Requirement for cotolerogenic gene products in the clonal deletion of I-E reactive T\u00a0cells","volume":"247","author":"Woodland","year":"1990","journal-title":"Science"},{"key":"10.1016\/j.celrep.2020.108320_bib56","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/349529a0","article-title":"An endogenous retrovirus mediating deletion of alpha beta T\u00a0cells?","volume":"349","author":"Woodland","year":"1991","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2020.108320_bib57","doi-asserted-by":"crossref","first-page":"167","DOI":"10.4049\/jimmunol.1900111","article-title":"Thymic development of a unique bone marrow\u2013resident innate-like T\u00a0cell subset with a potent innate immune function","volume":"203","author":"Yamamoto","year":"2019","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib58","doi-asserted-by":"crossref","first-page":"284","DOI":"10.4049\/jimmunol.1000679","article-title":"Fine-scale staging of T\u00a0cell lineage commitment in adult mouse thymus","volume":"185","author":"Yui","year":"2010","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.celrep.2020.108320_bib59","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1038\/s41467-019-09234-6","article-title":"Metascape provides a biologist-oriented resource for the analysis of systems-level datasets","volume":"10","author":"Zhou","year":"2019","journal-title":"Nat. Commun."}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124720313097?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124720313097?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T10:39:42Z","timestamp":1778841582000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124720313097"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":60,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["S2211124720313097"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2020.108320","relation":{},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2020,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In\u00a0Vitro Recapitulation of Murine Thymopoiesis from Single Hematopoietic Stem Cells","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2020.108320","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors.","name":"copyright","label":"Copyright"}],"article-number":"108320"}}