{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:32:51Z","timestamp":1790695971561,"version":"4.1.0"},"reference-count":66,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T00:00:00Z","timestamp":1633910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1DP5OD024583"],"award-info":[{"award-number":["1DP5OD024583"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DP2 AG058488-01"],"award-info":[{"award-number":["DP2 AG058488-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000051","name":"National Human Genome Research Institute","doi-asserted-by":"publisher","award":["R01HG010647"],"award-info":[{"award-number":["R01HG010647"]}],"id":[{"id":"10.13039\/100000051","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000051","name":"National Human Genome Research Institute","doi-asserted-by":"publisher","award":["R01"],"award-info":[{"award-number":["R01"]}],"id":[{"id":"10.13039\/100000051","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01 HD056034"],"award-info":[{"award-number":["R01 HD056034"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000070","name":"National Institute of Biomedical Imaging and Bioengineering","doi-asserted-by":"publisher","award":["2T32EB001680"],"award-info":[{"award-number":["2T32EB001680"]}],"id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013114","name":"Broad Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013114","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012756","name":"Lalor Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012756","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2021,11]]},"DOI":"10.1016\/j.celrep.2021.109915","type":"journal-article","created":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T01:43:55Z","timestamp":1635903835000},"page":"109915","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":135,"title":["Dissecting mammalian spermatogenesis using spatial transcriptomics"],"prefix":"10.1016","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7179-3229","authenticated-orcid":false,"given":"Haiqi","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan","family":"Murray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anubhav","family":"Sinha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anisha","family":"Laumas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jilong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9982-7344","authenticated-orcid":false,"given":"Daniel","family":"Lesman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xichen","family":"Nie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim","family":"Hotaling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingtao","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley R.","family":"Cairns","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan Z.","family":"Macosko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3117-3791","authenticated-orcid":false,"given":"C. Yan","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2021.109915_bib1","doi-asserted-by":"crossref","first-page":"548","DOI":"10.7150\/ijms.30097","article-title":"MALAT1 as a Diagnostic and Therapeutic Target in Diabetes-Related Complications: A Promising Long-Noncoding RNA","volume":"16","author":"Abdulle","year":"2019","journal-title":"Int. J. Med. Sci."},{"key":"10.1016\/j.celrep.2021.109915_bib2","doi-asserted-by":"crossref","first-page":"e1007233","DOI":"10.1371\/journal.pgen.1007233","article-title":"RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes","volume":"14","author":"Adams","year":"2018","journal-title":"PLoS Genet."},{"key":"10.1016\/j.celrep.2021.109915_bib68","doi-asserted-by":"crossref","first-page":"eaax2656","DOI":"10.1126\/science.aax2656","article-title":"Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems","volume":"371","author":"Alon","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.celrep.2021.109915_bib3","doi-asserted-by":"crossref","first-page":"e23992","DOI":"10.4161\/tisb.23992","article-title":"Diabetes, insulin-mediated glucose metabolism and Sertoli\/blood-testis barrier function","volume":"1","author":"Alves","year":"2013","journal-title":"Tissue Barriers"},{"key":"10.1016\/j.celrep.2021.109915_bib4","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1093\/humupd\/dmx036","article-title":"Testicular organoids: a new model to study the testicular microenvironment in\u00a0vitro?","volume":"24","author":"Alves-Lopes","year":"2018","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.celrep.2021.109915_bib5","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1172\/JCI117790","article-title":"Increased expression of mitochondrial-encoded genes in skeletal muscle of humans with diabetes mellitus","volume":"95","author":"Antonetti","year":"1995","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.celrep.2021.109915_bib6","doi-asserted-by":"crossref","first-page":"44","DOI":"10.3109\/19396360903490790","article-title":"Histone H4 acetylation is essential to proceed from a histone- to a protamine-based chromatin structure in spermatid nuclei of Drosophila melanogaster","volume":"56","author":"Awe","year":"2010","journal-title":"Syst Biol Reprod Med"},{"key":"10.1016\/j.celrep.2021.109915_bib7","doi-asserted-by":"crossref","first-page":"R55","DOI":"10.1530\/REP-15-0562","article-title":"Epigenetic regulation of the histone-to-protamine transition during spermiogenesis","volume":"151","author":"Bao","year":"2016","journal-title":"Reproduction"},{"key":"10.1016\/j.celrep.2021.109915_bib8","doi-asserted-by":"crossref","first-page":"302","DOI":"10.2164\/jandrol.05133","article-title":"Influence of a leptin deficiency on testicular morphology, germ cell apoptosis, and expression levels of apoptosis-related genes in the mouse","volume":"27","author":"Bhat","year":"2006","journal-title":"J.\u00a0Androl."},{"key":"10.1016\/j.celrep.2021.109915_bib9","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/nature10532","article-title":"The evolution of gene expression levels in mammalian organs","volume":"478","author":"Brawand","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2021.109915_bib64","article-title":"Robust decomposition of cell type mixtures in spatial transcriptomics","author":"Cable","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.celrep.2021.109915_bib10","series-title":"Male Hypogonadism","first-page":"49","article-title":"Human Spermatogenesis and Its Regulation","author":"Chen","year":"2017"},{"key":"10.1016\/j.celrep.2021.109915_bib11","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1242\/dev.165753","article-title":"Third-generation in situ hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust","volume":"145","author":"Choi","year":"2018","journal-title":"Development"},{"key":"10.1016\/j.celrep.2021.109915_bib12","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/S0015-0282(16)37153-9","article-title":"Duration of the cycle of the seminiferous epithelium in the mouse and hamster determined by means of 3H-thymidine and radioautography","volume":"20","author":"Clermont","year":"1969","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.celrep.2021.109915_bib13","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1095\/biolreprod67.1.220","article-title":"Altered meiotic regulation in oocytes from diabetic mice","volume":"67","author":"Colton","year":"2002","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.celrep.2021.109915_bib14","series-title":"Encyclopedia of Life Sciences","article-title":"Meiotic Sex Chromosome Inactivation","author":"Daish","year":"2010"},{"key":"10.1016\/j.celrep.2021.109915_bib15","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1242\/dev.146571","article-title":"The nature and dynamics of spermatogonial stem cells","volume":"144","author":"de Rooij","year":"2017","journal-title":"Development"},{"key":"10.1016\/j.celrep.2021.109915_bib16","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/j.celrep.2015.07.015","article-title":"Macrophages Contribute to the Spermatogonial Niche in the Adult Testis","volume":"12","author":"DeFalco","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2021.109915_bib17","doi-asserted-by":"crossref","first-page":"948","DOI":"10.4103\/1008-682X.150844","article-title":"The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis","volume":"17","author":"Ding","year":"2015","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.celrep.2021.109915_bib18","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1093\/molehr\/gau018","article-title":"The human testis-specific proteome defined by transcriptomics and antibody-based profiling","volume":"20","author":"Djureinovic","year":"2014","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.celrep.2021.109915_bib19","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.celrep.2021.109915_bib20","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/j.devcel.2018.07.025","article-title":"A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq","volume":"46","author":"Green","year":"2018","journal-title":"Dev. Cell"},{"key":"10.1016\/j.celrep.2021.109915_bib21","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1093\/biolre\/ioy027","article-title":"50 years of spermatogenesis: Sertoli cells and their interactions with germ cells","volume":"99","author":"Griswold","year":"2018","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.celrep.2021.109915_bib22","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1038\/s41422-018-0099-2","article-title":"The adult human testis transcriptional cell atlas","volume":"28","author":"Guo","year":"2018","journal-title":"Cell Res."},{"key":"10.1016\/j.celrep.2021.109915_bib23","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1186\/s13059-019-1874-1","article-title":"Normalization and variance stabilization of single-cell RNA-seq data using regularized negative binomial regression","volume":"20","author":"Hafemeister","year":"2019","journal-title":"Genome Biol."},{"key":"10.1016\/j.celrep.2021.109915_bib24","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1016\/j.celrep.2018.10.026","article-title":"The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids","volume":"25","author":"Hermann","year":"2018","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2021.109915_bib25","doi-asserted-by":"crossref","first-page":"3659","DOI":"10.1242\/dev.152413","article-title":"Unraveling transcriptome dynamics in human spermatogenesis","volume":"144","author":"Jan","year":"2017","journal-title":"Development"},{"key":"10.1016\/j.celrep.2021.109915_bib26","doi-asserted-by":"crossref","first-page":"147","DOI":"10.2174\/1573399810666140606111745","article-title":"Diabetes mellitus induced impairment of male reproductive functions: a review","volume":"10","author":"Jangir","year":"2014","journal-title":"Curr. Diabetes Rev."},{"key":"10.1016\/j.celrep.2021.109915_bib27","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1093\/biolre\/ioy031","article-title":"Leydig cell genes change their expression and association with polysomes in a stage-specific manner in the adult mouse testis","volume":"98","author":"Jauregui","year":"2018","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.celrep.2021.109915_bib28","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1242\/jcs.202721","article-title":"TSSK6 is required for \u03b3H2AX formation and the histone-to-protamine transition during spermiogenesis","volume":"130","author":"Jha","year":"2017","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2021.109915_bib29","doi-asserted-by":"crossref","first-page":"8315","DOI":"10.1073\/pnas.0709854105","article-title":"Stage-specific gene expression is a fundamental characteristic of rat spermatogenic cells and Sertoli cells","volume":"105","author":"Johnston","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2021.109915_bib30","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1177\/1933719107306228","article-title":"Disordered meiotic regulation of oocytes by duration of diabetes mellitus in BBdp rat","volume":"14","author":"Kim","year":"2007","journal-title":"Reprod. Sci."},{"key":"10.1016\/j.celrep.2021.109915_bib31","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1095\/biolreprod.115.137620","article-title":"Murine and Human Spermatids Are Characterized by Numerous, Newly Synthesized and Differentially Expressed Transcription Factors and Bromodomain-Containing Proteins","volume":"95","author":"Klaus","year":"2016","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.celrep.2021.109915_bib32","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1095\/biolreprod.107.064162","article-title":"DNA damage response during chromatin remodeling in elongating spermatids of mice","volume":"78","author":"Leduc","year":"2008","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.celrep.2021.109915_bib65","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1093\/bioinformatics\/btaa769","article-title":"Identifying signaling genes in spatial single-cell expression data","volume":"37","author":"Li","year":"2021","journal-title":"Bioinformatics"},{"key":"10.1016\/j.celrep.2021.109915_bib33","doi-asserted-by":"crossref","first-page":"2666","DOI":"10.1073\/pnas.1519395113","article-title":"Regulation of seminiferous tubule-associated stem Leydig cells in adult rat testes","volume":"113","author":"Li","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2021.109915_bib34","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1111\/j.1749-6632.1991.tb27318.x","article-title":"Expression of receptors during the cycle of the seminiferous epithelium","volume":"637","author":"Linder","year":"1991","journal-title":"Ann. N Y Acad. Sci."},{"key":"10.1016\/j.celrep.2021.109915_bib35","doi-asserted-by":"crossref","first-page":"180192","DOI":"10.1038\/sdata.2018.192","article-title":"Single-cell RNA sequencing of adult mouse testes","volume":"5","author":"Lukassen","year":"2018","journal-title":"Sci. Data"},{"key":"10.1016\/j.celrep.2021.109915_bib36","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1002\/stem.1238","article-title":"Concise review: Spermatogenesis in an artificial three-dimensional system","volume":"30","author":"Mahmoud","year":"2012","journal-title":"Stem Cells"},{"key":"10.1016\/j.celrep.2021.109915_bib37","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1095\/biolreprod.103.022541","article-title":"Transient DNA strand breaks during mouse and human spermiogenesis new insights in stage specificity and link to chromatin remodeling","volume":"70","author":"Marcon","year":"2004","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.celrep.2021.109915_bib38","doi-asserted-by":"crossref","first-page":"13074","DOI":"10.1038\/s41598-019-49600-4","article-title":"Hyperglycemia induces spermatogenic disruption via major pathways of diabetes pathogenesis","volume":"9","author":"Maresch","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.celrep.2021.109915_bib39","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1002\/mrd.1080310303","article-title":"Highly acetylated H4 is associated with histone displacement in rat spermatids","volume":"31","author":"Meistrich","year":"1992","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.celrep.2021.109915_bib40","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1126\/science.aaa0355","article-title":"Human genomics. The human transcriptome across tissues and individuals","volume":"348","author":"Mel\u00e9","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.celrep.2021.109915_bib41","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/gtc.12129","article-title":"Human cell growth regulator Ly-1 antibody reactive homologue accelerates processing of preribosomal RNA","volume":"19","author":"Miyazawa","year":"2014","journal-title":"Genes Cells"},{"key":"10.1016\/j.celrep.2021.109915_bib42","doi-asserted-by":"crossref","first-page":"2829","DOI":"10.1084\/jem.20170829","article-title":"Developmental origin and maintenance of distinct testicular macrophage populations","volume":"214","author":"Mossadegh-Keller","year":"2017","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.celrep.2021.109915_bib43","doi-asserted-by":"crossref","first-page":"e979623","DOI":"10.4161\/21565562.2014.979623","article-title":"Mechanisms of spermiogenesis and spermiation and how they are disturbed","volume":"4","author":"O\u2019Donnell","year":"2015","journal-title":"Spermatogenesis"},{"key":"10.1016\/j.celrep.2021.109915_bib67","doi-asserted-by":"crossref","first-page":"eaay3446","DOI":"10.1126\/science.aay3446","article-title":"In situ genome sequencing resolves DNA sequence and structure in intact biological samples","volume":"371","author":"Payne","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.celrep.2021.109915_bib44","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1098\/rstb.2010.0026","article-title":"Spermatogonial stem cell regulation and spermatogenesis","volume":"365","author":"Phillips","year":"2010","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"10.1016\/j.celrep.2021.109915_bib45","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1038\/nmeth.4402","article-title":"Reversed graph embedding resolves complex single-cell trajectories","volume":"14","author":"Qiu","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.celrep.2021.109915_bib46","first-page":"155","article-title":"Chromatin dynamics during spermiogenesis. Biochimica et Biophysica Acta (BBA) -","volume":"1839","author":"Rathke","year":"2014","journal-title":"Gene Regulatory Mechanisms"},{"key":"10.1016\/j.celrep.2021.109915_bib48","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.celrep.2021.109915_bib49","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1126\/science.aaw1219","article-title":"Slide-seq: A scalable technology for measuring genome-wide expression at high spatial resolution","volume":"363","author":"Rodriques","year":"2019","journal-title":"Science"},{"key":"10.1016\/j.celrep.2021.109915_bib50","doi-asserted-by":"crossref","first-page":"17312","DOI":"10.1002\/jcb.28995","article-title":"Impaired spermatogenesis associated with changes in spatial arrangement of Sertoli and spermatogonial cells following induced diabetes","volume":"120","author":"Sajadi","year":"2019","journal-title":"J.\u00a0Cell. Biochem."},{"key":"10.1016\/j.celrep.2021.109915_bib52","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.it.2015.05.002","article-title":"MERFISHing for spatial context","volume":"36","author":"Shalek","year":"2015","journal-title":"Trends Immunol."},{"key":"10.1016\/j.celrep.2021.109915_bib63","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.devcel.2020.05.010","article-title":"Single-Cell RNA Sequencing of Human, Macaque, and Mouse Testes Uncovers Conserved and Divergent Features of Mammalian Spermatogenesis","volume":"54","author":"Shami","year":"2020","journal-title":"Dev. Cell"},{"key":"10.1016\/j.celrep.2021.109915_bib53","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.semcdb.2014.02.012","article-title":"The regulation of spermatogenesis by androgens","volume":"30","author":"Smith","year":"2014","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.celrep.2021.109915_bib54","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/s41587-020-0739-1","article-title":"Highly sensitive spatial transcriptomics at near-cellular resolution with Slide-seqV2.","volume":"39","author":"Stickels","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.celrep.2021.109915_bib55","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1016\/j.cell.2019.05.031","article-title":"Comprehensive Integration of Single-Cell Data","volume":"177","author":"Stuart","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2021.109915_bib56","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.mod.2011.12.003","article-title":"Retinoic acid metabolism links the periodical differentiation of germ cells with the cycle of Sertoli cells in mouse seminiferous epithelium","volume":"128","author":"Sugimoto","year":"2012","journal-title":"Mech. Dev."},{"key":"10.1016\/j.celrep.2021.109915_bib57","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1038\/s41592-019-0701-7","article-title":"Statistical analysis of spatial expression patterns for spatially resolved transcriptomic studies","volume":"17","author":"Sun","year":"2020","journal-title":"Nat. Methods"},{"key":"10.1016\/j.celrep.2021.109915_bib58","doi-asserted-by":"crossref","first-page":"e1004964","DOI":"10.1371\/journal.pgen.1004964","article-title":"Contribution of the two genes encoding histone variant h3.3 to viability and fertility in mice","volume":"11","author":"Tang","year":"2015","journal-title":"PLoS Genet."},{"key":"10.1016\/j.celrep.2021.109915_bib66","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.semcdb.2016.01.004","article-title":"Regulation of microtubule (MT)-based cytoskeleton in the seminiferous epithelium during spermatogenesis","volume":"59","author":"Tang","year":"2016","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.celrep.2021.109915_bib59","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1242\/dev.000018","article-title":"Meiotic sex chromosome inactivation","volume":"134","author":"Turner","year":"2007","journal-title":"Development"},{"key":"10.1016\/j.celrep.2021.109915_bib60","doi-asserted-by":"crossref","first-page":"1260419","DOI":"10.1126\/science.1260419","article-title":"Proteomics. Tissue-based map of the human proteome","volume":"347","author":"Uhl\u00e9n","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.celrep.2021.109915_bib61","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.stem.2018.08.007","article-title":"Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis","volume":"23","author":"Wang","year":"2018","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.celrep.2021.109915_bib62","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"clusterProfiler: an R package for comparing biological themes among gene clusters","volume":"16","author":"Yu","year":"2012","journal-title":"OMICS"}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124721013887?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124721013887?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T10:45:20Z","timestamp":1778841920000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124721013887"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":66,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["S2211124721013887"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2021.109915","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.10.17.343335","asserted-by":"object"}]},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dissecting mammalian spermatogenesis using spatial transcriptomics","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2021.109915","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Author(s).","name":"copyright","label":"Copyright"}],"article-number":"109915"}}