{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T12:46:40Z","timestamp":1792414000468,"version":"4.4.0"},"reference-count":77,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T00:00:00Z","timestamp":1643932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["U19 AI109633"],"award-info":[{"award-number":["U19 AI109633"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01 AI140868"],"award-info":[{"award-number":["R01 AI140868"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012692","name":"Yerkes National Primate Research Center, Emory University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012692","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016958","name":"Office of Research Infrastructure Programs, National Institutes of Health","doi-asserted-by":"publisher","award":["P51 OD011132"],"award-info":[{"award-number":["P51 OD011132"]}],"id":[{"id":"10.13039\/100016958","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.1016\/j.celrep.2022.110436","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T12:02:14Z","timestamp":1646136134000},"page":"110436","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"title":["Structure-guided changes at the V2 apex of HIV-1 clade C trimer enhance elicitation of autologous neutralizing and broad V1V2-scaffold antibodies"],"prefix":"10.1016","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6903-969X","authenticated-orcid":false,"given":"Anusmita","family":"Sahoo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar A.","family":"Hodge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celia C.","family":"LaBranche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0102-6566","authenticated-orcid":false,"given":"Tiffany M.","family":"Styles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6072-4383","authenticated-orcid":false,"given":"Narayanaiah","family":"Cheedarla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayalnesh","family":"Shiferaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Ozorowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Hsin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7153-3769","authenticated-orcid":false,"given":"Andrew B.","family":"Ward","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia D.","family":"Tomaras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0856-6319","authenticated-orcid":false,"given":"David C.","family":"Montefiori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darrell J.","family":"Irvine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly K.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6309-6797","authenticated-orcid":false,"given":"Rama Rao","family":"Amara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2022.110436_bib1","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1038\/s41591-020-0858-8","article-title":"T cell-inducing vaccine durably prevents mucosal SHIV infection even with lower neutralizing antibody titers","volume":"26","author":"Arunachalam","year":"2020","journal-title":"Nat. Med."},{"key":"10.1016\/j.celrep.2022.110436_bib2","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.3389\/fimmu.2018.01116","article-title":"Cleavage-independent HIV-1 trimers from CHO cell lines elicit robust autologous tier 2 neutralizing antibodies","volume":"9","author":"Bale","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.celrep.2022.110436_bib3","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nature10766","article-title":"Vaccine protection against acquisition of neutralization-resistant SIV challenges in rhesus monkeys","volume":"482","author":"Barouch","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2022.110436_bib4","doi-asserted-by":"crossref","first-page":"e366","DOI":"10.1016\/S2352-3018(18)30071-7","article-title":"Subtype C ALVAC-HIV and bivalent subtype C gp120\/MF59 HIV-1 vaccine in low-risk, HIV-uninfected, South African adults: a phase 1\/2 trial","volume":"5","author":"Bekker","year":"2018","journal-title":"Lancet HIV"},{"key":"10.1016\/j.celrep.2022.110436_bib5","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1089\/aid.2019.0239","article-title":"A systematic approach to HIV-1 vaccine immunogen selection","volume":"36","author":"Bontempo","year":"2020","journal-title":"AIDS Res. Hum. Retrovir."},{"key":"10.1016\/j.celrep.2022.110436_bib6","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.chom.2018.12.001","article-title":"HIV-1 neutralizing antibody signatures and application to epitope-targeted vaccine design","volume":"25","author":"Bricault","year":"2019","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.celrep.2022.110436_bib7","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01846-18","article-title":"Clade C HIV-1 envelope vaccination regimens differ in their ability to elicit antibodies with moderate neutralization breadth against genetically diverse tier 2 HIV-1 envelope variants","volume":"93","author":"Burton","year":"2019","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2022.110436_bib8","doi-asserted-by":"crossref","first-page":"3152","DOI":"10.1073\/pnas.1314718111","article-title":"Tyrosine sulfation in the second variable loop (V2) of HIV-1 gp120 stabilizes V2-V3 interaction and modulates neutralization sensitivity","volume":"111","author":"Cimbro","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.celrep.2022.110436_bib9","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1016\/j.cell.2015.11.056","article-title":"Immunogenicity of stabilized HIV-1 envelope trimers with reduced exposure of non-neutralizing epitopes","volume":"163","author":"de Taeye","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2022.110436_bib10","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1128\/JVI.02853-13","article-title":"Global panel of HIV-1 Env reference strains for standardized assessments of vaccine-elicited neutralizing antibodies","volume":"88","author":"deCamp","year":"2014","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2022.110436_bib11","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nature13036","article-title":"Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies","volume":"509","author":"Doria-Rose","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2022.110436_bib12","doi-asserted-by":"crossref","first-page":"e1006614","DOI":"10.1371\/journal.ppat.1006614","article-title":"Targeted N-glycan deletion at the receptor-binding site retains HIV Env NFL trimer integrity and accelerates the elicited antibody response","volume":"13","author":"Dubrovskaya","year":"2017","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2022.110436_bib13","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/j.immuni.2019.10.008","article-title":"Vaccination with glycan-modified HIV NFL envelope trimer-liposomes elicits broadly neutralizing antibodies to multiple sites of vulnerability","volume":"51","author":"Dubrovskaya","year":"2019","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2022.110436_bib14","doi-asserted-by":"crossref","first-page":"eabk1533","DOI":"10.1126\/scitranslmed.abk1533","article-title":"Sequential immunization of macaques elicits heterologous neutralizing antibodies targeting the V3-glycan patch of HIV-1 Env","volume":"13","author":"Escolano","year":"2021","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.celrep.2022.110436_bib15","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1016\/j.cell.2016.07.030","article-title":"Sequential immunization elicits broadly neutralizing anti-HIV-1 antibodies in Ig knockin mice","volume":"166","author":"Escolano","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2022.110436_bib16","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1128\/CVI.00230-14","article-title":"Nonneutralizing functional antibodies: a new \"old\" paradigm for HIV vaccines","volume":"21","author":"Excler","year":"2014","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.celrep.2022.110436_bib17","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1016\/j.immuni.2014.04.009","article-title":"Broadly neutralizing HIV antibodies define a glycan-dependent epitope on the prefusion conformation of gp41 on cleaved envelope trimers","volume":"40","author":"Falkowska","year":"2014","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2022.110436_bib18","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1086\/598502","article-title":"Effect of HIV-1 subtype on virologic and immunologic response to starting highly active antiretroviral therapy","volume":"48","author":"Geretti","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.celrep.2022.110436_bib19","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.virol.2012.02.003","article-title":"Functional and immunochemical cross-reactivity of V2-specific monoclonal antibodies from HIV-1-infected individuals","volume":"427","author":"Gorny","year":"2012","journal-title":"Virology"},{"key":"10.1016\/j.celrep.2022.110436_bib20","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1056\/NEJMoa2031499","article-title":"Vaccine efficacy of ALVAC-HIV and bivalent subtype C gp120-MF59 in adults","volume":"384","author":"Gray","year":"2021","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.celrep.2022.110436_bib21","doi-asserted-by":"crossref","first-page":"eaax1880","DOI":"10.1126\/scitranslmed.aax1880","article-title":"Immune correlates of the Thai RV144 HIV vaccine regimen in South Africa","volume":"11","author":"Gray","year":"2019","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.celrep.2022.110436_bib22","doi-asserted-by":"crossref","first-page":"e1004570","DOI":"10.1371\/journal.ppat.1004570","article-title":"Well-ordered trimeric HIV-1 subtype B and C soluble spike mimetics generated by negative selection display native-like properties","volume":"11","author":"Guenaga","year":"2015","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2022.110436_bib23","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.immuni.2017.04.014","article-title":"Glycine substitution at helix-to-coil transitions facilitates the structural determination of a stabilized subtype C HIV envelope glycoprotein","volume":"46","author":"Guenaga","year":"2017","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2022.110436_bib24","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1016\/j.str.2014.05.001","article-title":"CD4-induced activation in a soluble HIV-1 Env trimer","volume":"22","author":"Guttman","year":"2014","journal-title":"Structure"},{"key":"10.1016\/j.celrep.2022.110436_bib25","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00955-18","article-title":"Structural constraints at the trimer apex stabilize the HIV-1 envelope in a closed, antibody-protected conformation","volume":"9","author":"Guzzo","year":"2018","journal-title":"mBio"},{"key":"10.1016\/j.celrep.2022.110436_bib26","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1056\/NEJMoa1113425","article-title":"Immune-correlates analysis of an HIV-1 vaccine efficacy trial","volume":"366","author":"Haynes","year":"2012","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.celrep.2022.110436_bib27","doi-asserted-by":"crossref","first-page":"eaau6769","DOI":"10.1126\/sciadv.aau6769","article-title":"HIV-1 vaccine design through minimizing envelope metastability","volume":"4","author":"He","year":"2018","journal-title":"Sci. Adv."},{"key":"10.1016\/j.celrep.2022.110436_bib28","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02198-17","article-title":"Reduced cell-associated DNA and improved viral control in macaques following passive transfer of a single anti-V2 monoclonal antibody and repeated simian\/human immunodeficiency virus challenges","volume":"92","author":"Hessell","year":"2018","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2022.110436_bib29","doi-asserted-by":"crossref","first-page":"5850","DOI":"10.1038\/s41467-020-19650-8","article-title":"Vaccine elicitation of HIV broadly neutralizing antibodies from engineered B cells","volume":"11","author":"Huang","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.celrep.2022.110436_bib30","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.ab.2013.12.002","article-title":"A rapid, efficient, and economical inverse polymerase chain reaction-based method for generating a site saturation mutant library","volume":"449","author":"Jain","year":"2014","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.celrep.2022.110436_bib31","doi-asserted-by":"crossref","first-page":"7285","DOI":"10.1128\/JVI.00481-16","article-title":"Vaccine-elicited mucosal and systemic antibody responses are associated with reduced simian immunodeficiency viremia in infant rhesus macaques","volume":"90","author":"Jensen","year":"2016","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2022.110436_bib32","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1038\/s41467-019-08739-4","article-title":"HIV-1 vaccination by needle-free oral injection induces strong mucosal immunity and protects against SHIV challenge","volume":"10","author":"Jones","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.celrep.2022.110436_bib33","doi-asserted-by":"crossref","first-page":"3586","DOI":"10.4049\/jimmunol.1600629","article-title":"High doses of GM-CSF inhibit antibody responses in rectal secretions and diminish modified vaccinia Ankara\/simian immunodeficiency virus vaccine protection in TRIM5alpha-restrictive macaques","volume":"197","author":"Kannanganat","year":"2016","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.celrep.2022.110436_bib34","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01844-16","article-title":"Adjuvanting a simian immunodeficiency virus vaccine with toll-like receptor ligands encapsulated in nanoparticles induces persistent antibody responses and enhanced protection in TRIM5alpha restrictive macaques","volume":"91","author":"Kasturi","year":"2017","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2022.110436_bib35","doi-asserted-by":"crossref","first-page":"e1006913","DOI":"10.1371\/journal.ppat.1006913","article-title":"Epitopes for neutralizing antibodies induced by HIV-1 envelope glycoprotein BG505 SOSIP trimers in rabbits and macaques","volume":"14","author":"Klasse","year":"2018","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2022.110436_bib36","doi-asserted-by":"crossref","first-page":"e1005864","DOI":"10.1371\/journal.ppat.1005864","article-title":"Sequential and simultaneous immunization of rabbits with HIV-1 envelope glycoprotein SOSIP.664 trimers from clades A, B and C","volume":"12","author":"Klasse","year":"2016","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2022.110436_bib37","doi-asserted-by":"crossref","first-page":"12040","DOI":"10.1038\/ncomms12040","article-title":"Uncleaved prefusion-optimized gp140 trimers derived from analysis of HIV-1 envelope metastability","volume":"7","author":"Kong","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.celrep.2022.110436_bib38","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1038\/nsmb.3051","article-title":"Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env","volume":"22","author":"Kwon","year":"2015","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.celrep.2022.110436_bib39","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jsb.2009.01.002","article-title":"Appion: an integrated, database-driven pipeline to facilitate EM image processing","volume":"166","author":"Lander","year":"2009","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.celrep.2022.110436_bib40","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/j.immuni.2017.03.017","article-title":"A broadly neutralizing antibody targets the dynamic HIV envelope trimer apex via a long, rigidified, and anionic beta-hairpin structure","volume":"46","author":"Lee","year":"2017","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2022.110436_bib41","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.immuni.2012.11.011","article-title":"Vaccine induction of antibodies against a structurally heterogeneous site of immune pressure within HIV-1 envelope protein variable regions 1 and 2","volume":"38","author":"Liao","year":"2013","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2022.110436_bib42","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1038\/nsmb.3382","article-title":"Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer","volume":"24","author":"Liu","year":"2017","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.celrep.2022.110436_bib43","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1128\/JVI.03608-14","article-title":"HIV-1 fitness cost associated with escape from the VRC01 class of CD4 binding site neutralizing antibodies","volume":"89","author":"Lynch","year":"2015","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2022.110436_bib44","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1097\/COH.0000000000000548","article-title":"Targeting broadly neutralizing antibody precursors: a naive approach to vaccine design","volume":"14","author":"McGuire","year":"2019","journal-title":"Curr. Opin. HIV AIDS"},{"key":"10.1016\/j.celrep.2022.110436_bib45","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/978-1-59745-170-3_26","article-title":"Measuring HIV neutralization in a luciferase reporter gene assay","volume":"485","author":"Montefiori","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.celrep.2022.110436_bib46","article-title":"Antibody pressure by a human monoclonal antibody targeting the 2009 pandemic H1N1 virus hemagglutinin drives the emergence of a virus with increased virulence in mice","volume":"3","author":"O'Donnell","year":"2012","journal-title":"mBio"},{"key":"10.1016\/j.celrep.2022.110436_bib47","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.jsb.2003.08.005","article-title":"Topology representing network enables highly accurate classification of protein images taken by cryo electron-microscope without masking","volume":"143","author":"Ogura","year":"2003","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.celrep.2022.110436_bib48","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1038\/nsmb.2600","article-title":"Structural basis for diverse N-glycan recognition by HIV-1-neutralizing V1-V2-directed antibody PG16","volume":"20","author":"Pancera","year":"2013","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.celrep.2022.110436_bib49","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/j.immuni.2017.05.007","article-title":"Elicitation of robust tier 2 neutralizing antibody responses in nonhuman primates by HIV envelope trimer immunization using optimized approaches","volume":"46","author":"Pauthner","year":"2017","journal-title":"Immunity"},{"key":"10.1016\/j.celrep.2022.110436_bib50","doi-asserted-by":"crossref","first-page":"11483","DOI":"10.1073\/pnas.1004600107","article-title":"Structure and function of broadly reactive antibody PG16 reveal an H3 subdomain that mediates potent neutralization of HIV-1","volume":"107","author":"Pejchal","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.celrep.2022.110436_bib51","doi-asserted-by":"crossref","first-page":"e0180720","DOI":"10.1371\/journal.pone.0180720","article-title":"V1V2-specific complement activating serum IgG as a correlate of reduced HIV-1 infection risk in RV144","volume":"12","author":"Perez","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.celrep.2022.110436_bib52","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera--a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.celrep.2022.110436_bib53","doi-asserted-by":"crossref","first-page":"e1005742","DOI":"10.1371\/journal.ppat.1005742","article-title":"Features of recently transmitted HIV-1 clade C viruses that impact antibody recognition: implications for active and passive immunization","volume":"12","author":"Rademeyer","year":"2016","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2022.110436_bib54","doi-asserted-by":"crossref","first-page":"2209","DOI":"10.1056\/NEJMoa0908492","article-title":"Vaccination with ALVAC and AIDSVAX to prevent HIV-1 infection in Thailand","volume":"361","author":"Rerks-Ngarm","year":"2009","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.celrep.2022.110436_bib55","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nature11519","article-title":"Increased HIV-1 vaccine efficacy against viruses with genetic signatures in Env V2","volume":"490","author":"Rolland","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2022.110436_bib56","doi-asserted-by":"crossref","first-page":"e1003618","DOI":"10.1371\/journal.ppat.1003618","article-title":"A next-generation cleaved, soluble HIV-1 Env trimer, BG505 SOSIP.664 gp140, expresses multiple epitopes for broadly neutralizing but not non-neutralizing antibodies","volume":"9","author":"Sanders","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2022.110436_bib57","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/imr.12481","article-title":"Native-like Env trimers as a platform for HIV-1 vaccine design","volume":"275","author":"Sanders","year":"2017","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.celrep.2022.110436_bib58","doi-asserted-by":"crossref","first-page":"aac4223","DOI":"10.1126\/science.aac4223","article-title":"HIV-1 VACCINES. HIV-1 neutralizing antibodies induced by native-like envelope trimers","volume":"349","author":"Sanders","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.celrep.2022.110436_bib59","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.celrep.2015.03.047","article-title":"Cleavage-independent HIV-1 Env trimers engineered as soluble native spike mimetics for vaccine design","volume":"11","author":"Sharma","year":"2015","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2022.110436_bib60","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.1038\/s41598-020-57491-z","article-title":"HIV-1 vaccine sequences impact V1V2 antibody responses: a comparison of two Poxvirus Prime gp120 boost vaccine regimens","volume":"10","author":"Shen","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.celrep.2022.110436_bib61","doi-asserted-by":"crossref","first-page":"17624","DOI":"10.1073\/pnas.1415789111","article-title":"Recombinant HIV envelope trimer selects for quaternary-dependent antibodies targeting the trimer apex","volume":"111","author":"Sok","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.1016\/j.celrep.2022.110436_bib62","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00934-19","article-title":"Human immunodeficiency virus C.1086 envelope gp140 protein boosts following DNA\/modified vaccinia virus Ankara vaccination fail to enhance heterologous anti-V1V2 antibody response and protection against clade C simian-human immunodeficiency virus challenge","volume":"93","author":"Styles","year":"2019","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2022.110436_bib63","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jsb.2005.03.010","article-title":"Automated molecular microscopy: the new Leginon system","volume":"151","author":"Suloway","year":"2005","journal-title":"J.\u00a0Struct. Biol."},{"key":"10.1016\/j.celrep.2022.110436_bib64","doi-asserted-by":"crossref","first-page":"e108446","DOI":"10.1371\/journal.pone.0108446","article-title":"Cryptic determinant of alpha4beta7 binding in the V2 loop of HIV-1 gp120","volume":"9","author":"Tassaneetrithep","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.celrep.2022.110436_bib65","doi-asserted-by":"crossref","first-page":"12449","DOI":"10.1128\/JVI.01708-08","article-title":"Initial B-cell responses to transmitted human immunodeficiency virus type 1: virion-binding immunoglobulin M (IgM) and IgG antibodies followed by plasma anti-gp41 antibodies with ineffective control of initial viremia","volume":"82","author":"Tomaras","year":"2008","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2022.110436_bib66","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1016\/j.celrep.2017.07.077","article-title":"Improving the immunogenicity of native-like HIV-1 envelope trimers by hyperstabilization","volume":"20","author":"Torrents de la Pena","year":"2017","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2022.110436_bib67","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1038\/nm.4105","article-title":"Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition","volume":"22","author":"Vaccari","year":"2016","journal-title":"Nat. Med."},{"key":"10.1016\/j.celrep.2022.110436_bib68","doi-asserted-by":"crossref","first-page":"3123","DOI":"10.1016\/j.celrep.2018.11.058","article-title":"V2-Directed vaccine-like antibodies from HIV-1 infection identify an additional K169-binding light chain motif with broad ADCC activity","volume":"25","author":"van Eeden","year":"2018","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2022.110436_bib69","doi-asserted-by":"crossref","first-page":"9471","DOI":"10.1128\/JVI.01351-16","article-title":"Epitope-independent purification of native-like envelope trimers from diverse HIV-1 isolates","volume":"90","author":"Verkerke","year":"2016","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.celrep.2022.110436_bib70","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.celrep.2017.09.024","article-title":"Elicitation of neutralizing antibodies targeting the V2 apex of the HIV envelope trimer in a wild-type Animal model","volume":"21","author":"Voss","year":"2017","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2022.110436_bib71","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1016\/j.jasms.2006.05.014","article-title":"Identification and characterization of EX1 kinetics in H\/D exchange mass spectrometry by peak width analysis","volume":"17","author":"Weis","year":"2006","journal-title":"J.\u00a0Am. Soc. Mass Spectrom."},{"key":"10.1016\/j.celrep.2022.110436_bib72","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/s41591-018-0042-6","article-title":"Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1","volume":"24","author":"Xu","year":"2018","journal-title":"Nat. Med."},{"key":"10.1016\/j.celrep.2022.110436_bib73","doi-asserted-by":"crossref","first-page":"228ra239","DOI":"10.1126\/scitranslmed.3007730","article-title":"Vaccine-induced Env V1-V2 IgG3 correlates with lower HIV-1 infection risk and declines soon after vaccination","volume":"6","author":"Yates","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.celrep.2022.110436_bib74","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1007\/s13361-017-1701-4","article-title":"Electron-transfer\/higher-energy collision dissociation (EThcD)-Enabled intact glycopeptide\/glycoproteome characterization","volume":"28","author":"Yu","year":"2017","journal-title":"J.\u00a0Am. Soc. Mass Spectrom."},{"key":"10.1016\/j.celrep.2022.110436_bib75","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1097\/COH.0000000000000551","article-title":"Vaccine-induced V1V2-specific antibodies control and or protect against infection with HIV, SIV and SHIV","volume":"14","author":"Zolla-Pazner","year":"2019","journal-title":"Curr. Opin. HIV AIDS"},{"key":"10.1016\/j.celrep.2022.110436_bib76","doi-asserted-by":"crossref","first-page":"e87572","DOI":"10.1371\/journal.pone.0087572","article-title":"Vaccine-induced IgG antibodies to V1V2 regions of multiple HIV-1 subtypes correlate with decreased risk of HIV-1 infection","volume":"9","author":"Zolla-Pazner","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.celrep.2022.110436_bib77","doi-asserted-by":"crossref","first-page":"e53629","DOI":"10.1371\/journal.pone.0053629","article-title":"Analysis of V2 antibody responses induced in vaccinees in the ALVAC\/AIDSVAX HIV-1 vaccine efficacy trial","volume":"8","author":"Zolla-Pazner","year":"2013","journal-title":"PLoS One"}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124722001632?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124722001632?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T08:30:04Z","timestamp":1777624204000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124722001632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":77,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["S2211124722001632"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2022.110436","relation":{},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structure-guided changes at the V2 apex of HIV-1 clade C trimer enhance elicitation of autologous neutralizing and broad V1V2-scaffold antibodies","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2022.110436","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors.","name":"copyright","label":"Copyright"}],"article-number":"110436"}}