{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:49:00Z","timestamp":1790768940982,"version":"4.1.0"},"reference-count":78,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T00:00:00Z","timestamp":1666915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20190081"],"award-info":[{"award-number":["BK20190081"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2702702"],"award-info":[{"award-number":["2022YFC2702702"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070842"],"award-info":[{"award-number":["32070842"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270899"],"award-info":[{"award-number":["32270899"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2022,11]]},"DOI":"10.1016\/j.celrep.2022.111683","type":"journal-article","created":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T10:37:27Z","timestamp":1669113447000},"page":"111683","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":44,"title":["Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella"],"prefix":"10.1016","volume":"41","author":[{"given":"Xin","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuang","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jintao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changtong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shushu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0201-0117","authenticated-orcid":false,"given":"Mingxi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2022.111683_bib1","doi-asserted-by":"crossref","first-page":"107s","DOI":"10.1083\/jcb.91.3.107s","article-title":"Cilia and flagella of eukaryotes","volume":"91","author":"Gibbons","year":"1981","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib2","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1146\/annurev.physiol.69.040705.141236","article-title":"Overview of structure and function of mammalian cilia","volume":"69","author":"Satir","year":"2007","journal-title":"Annu. Rev. Physiol."},{"key":"10.1016\/j.celrep.2022.111683_bib3","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1083\/jcb.201011152","article-title":"Evolution: tracing the origins of centrioles, cilia, and flagella","volume":"194","author":"Carvalho-Santos","year":"2011","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib4","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1093\/molehr\/gar034","article-title":"Sperm flagella: comparative and phylogenetic perspectives of protein components","volume":"17","author":"Inaba","year":"2011","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.celrep.2022.111683_bib5","doi-asserted-by":"crossref","first-page":"F37","DOI":"10.1083\/jcb.151.5.F37","article-title":"The 9\u00a0+ 2 axoneme anchors multiple inner arm dyneins and a network of kinases and phosphatases that control motility","volume":"151","author":"Porter","year":"2000","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib6","doi-asserted-by":"crossref","first-page":"50","DOI":"10.4161\/bioa.20394","article-title":"Axonemal radial spokes: 3D structure, function and assembly","volume":"2","author":"Pigino","year":"2012","journal-title":"BioArchitecture"},{"key":"10.1016\/j.celrep.2022.111683_bib7","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1083\/jcb.200908067","article-title":"The dynein regulatory complex is the nexin link and a major regulatory node in cilia and flagella","volume":"187","author":"Heuser","year":"2009","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib8","doi-asserted-by":"crossref","first-page":"eaar1968","DOI":"10.1126\/science.aar1968","article-title":"Asymmetric distribution and spatial switching of dynein activity generates ciliary motility","volume":"360","author":"Lin","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.celrep.2022.111683_bib9","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1002\/cm.970240403","article-title":"Flagellar radial spoke: a model molecular genetic system for studying organelle assembly","volume":"24","author":"Curry","year":"1993","journal-title":"Cell Motil Cytoskeleton"},{"key":"10.1016\/j.celrep.2022.111683_bib10","doi-asserted-by":"crossref","first-page":"2008","DOI":"10.1083\/jcb.100.6.2008","article-title":"Substructure of inner dynein arms, radial spokes, and the central pair\/projection complex of cilia and flagella","volume":"100","author":"Goodenough","year":"1985","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib11","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1083\/jcb.201106125","article-title":"Cryoelectron tomography of radial spokes in cilia and flagella","volume":"195","author":"Pigino","year":"2011","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib12","doi-asserted-by":"crossref","first-page":"15889","DOI":"10.1073\/pnas.0508274102","article-title":"3D structure of eukaryotic flagella in a quiescent state revealed by cryo-electron tomography","volume":"102","author":"Nicastro","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2022.111683_bib13","doi-asserted-by":"crossref","first-page":"5727","DOI":"10.1038\/ncomms6727","article-title":"Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia","volume":"5","author":"Lin","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.celrep.2022.111683_bib14","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1008664","article-title":"Rsph4a is essential for the triplet radial spoke head assembly of the mouse motile cilia","volume":"16","author":"Yoke","year":"2020","journal-title":"PLoS Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib15","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1091\/mbc.e11-08-0692","article-title":"Three-dimensional structure of the radial spokes reveals heterogeneity and interactions with dyneins in Chlamydomonas flagella","volume":"23","author":"Barber","year":"2012","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.celrep.2022.111683_bib16","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1083\/jcb.88.1.73","article-title":"Radial spokes of Chlamydomonas flagella: polypeptide composition and phosphorylation of stalk components","volume":"88","author":"Piperno","year":"1981","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib18","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1242\/jcs.02811","article-title":"Radial spoke proteins of Chlamydomonas flagella","volume":"119","author":"Yang","year":"2006","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2022.111683_bib17","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2021180118","article-title":"Distinct architecture and composition of mouse axonemal radial spoke head revealed by cryo-EM","volume":"118","author":"Zheng","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2022.111683_bib19","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/(SICI)1097-0169(1996)33:2<88::AID-CM2>3.0.CO;2-E","article-title":"Ability of paralyzed flagella mutants of Chlamydomonas to move","volume":"33","author":"Omoto","year":"1996","journal-title":"Cell Motil Cytoskeleton"},{"key":"10.1016\/j.celrep.2022.111683_bib20","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1002\/(SICI)1097-0169(1997)38:1<91::AID-CM8>3.0.CO;2-K","article-title":"Reactivation at low ATP distinguishes among classes of paralyzed flagella mutants","volume":"38","author":"Frey","year":"1997","journal-title":"Cell Motil Cytoskeleton"},{"key":"10.1016\/j.celrep.2022.111683_bib21","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1002\/1097-0169(200007)46:3<190::AID-CM4>3.0.CO;2-#","article-title":"Vigorous beating of Chlamydomonas axonemes lacking central pair\/radial spoke structures in the presence of salts and organic compounds","volume":"46","author":"Yagi","year":"2000","journal-title":"Cell Motil Cytoskeleton"},{"key":"10.1016\/j.celrep.2022.111683_bib22","doi-asserted-by":"crossref","first-page":"a018325","DOI":"10.1101\/cshperspect.a018325","article-title":"Ciliary motility: regulation of axonemal dynein motors","volume":"9","author":"Viswanadha","year":"2017","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib23","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1083\/jcb.88.1.80","article-title":"Radial spokes of Chlamydomonas flagella: genetic analysis of assembly and function","volume":"88","author":"Huang","year":"1981","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib24","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1083\/jcb.76.3.729","article-title":"Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components","volume":"76","author":"Witman","year":"1978","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib25","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1002\/cm.21171","article-title":"Characterizing the ultrastructure of primary ciliary dyskinesia transposition defect using electron tomography","volume":"71","author":"Burgoyne","year":"2014","journal-title":"Cytoskeleton"},{"key":"10.1016\/j.celrep.2022.111683_bib26","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.ajhg.2009.01.011","article-title":"Mutations in radial spoke head protein genes RSPH9 and RSPH4A cause primary ciliary dyskinesia with central-microtubular-pair abnormalities","volume":"84","author":"Castleman","year":"2009","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib27","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1164\/rccm.201311-2047OC","article-title":"Mutations in RSPH1 cause primary ciliary dyskinesia with a unique clinical and ciliary phenotype","volume":"189","author":"Knowles","year":"2014","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.celrep.2022.111683_bib28","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1165\/rcmb.2014-0483OC","article-title":"Immunofluorescence analysis and diagnosis of primary ciliary dyskinesia with radial spoke defects","volume":"53","author":"Frommer","year":"2015","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib29","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/S2213-2600(19)30374-1","article-title":"Primary ciliary dyskinesia in the genomics age","volume":"8","author":"Lucas","year":"2020","journal-title":"Lancet Respir. Med."},{"key":"10.1016\/j.celrep.2022.111683_bib30","series-title":"GeneReviews(\u00ae)","article-title":"Primary ciliary dyskinesia","author":"Zariwala","year":"1993"},{"key":"10.1016\/j.celrep.2022.111683_bib31","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1111\/cge.13742","article-title":"A nonsense variant in NME5 causes human primary ciliary dyskinesia with radial spoke defects","volume":"98","author":"Cho","year":"2020","journal-title":"Clin. Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib32","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1002\/humu.22371","article-title":"Founder mutation in RSPH4A identified in patients of Hispanic descent with primary ciliary dyskinesia","volume":"34","author":"Daniels","year":"2013","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.celrep.2022.111683_bib33","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.ajhg.2015.05.004","article-title":"RSPH3 mutations cause primary ciliary dyskinesia with central-complex defects and a near absence of radial spokes","volume":"97","author":"Jeanson","year":"2015","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib34","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.ajhg.2013.07.013","article-title":"Loss-of-function mutations in RSPH1 cause primary ciliary dyskinesia with central-complex and radial-spoke defects","volume":"93","author":"Kott","year":"2013","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib35","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.ajhg.2016.06.022","article-title":"Mutations in DNAJB13, encoding an HSP40 family member, cause primary ciliary dyskinesia and male infertility","volume":"99","author":"El Khouri","year":"2016","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib36","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1136\/jmedgenet-2019-106775","article-title":"Biallelic variants in MAATS1 encoding CFAP91, a calmodulin-associated and spoke-associated complex protein, cause severe astheno-teratozoospermia and male infertility","volume":"57","author":"Martinez","year":"2020","journal-title":"J.\u00a0Med. Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib37","article-title":"The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development","volume":"147","author":"Beckers","year":"2020","journal-title":"Development"},{"key":"10.1016\/j.celrep.2022.111683_bib38","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1016\/j.febslet.2009.06.016","article-title":"Proteomic characterization of sperm radial spokes identifies a novel spoke protein with an ubiquitin domain","volume":"583","author":"Satouh","year":"2009","journal-title":"FEBS Lett."},{"key":"10.1016\/j.celrep.2022.111683_bib39","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1038\/s41594-020-00530-0","article-title":"Structures of radial spokes and associated complexes important for ciliary motility","volume":"28","author":"Gui","year":"2021","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib40","doi-asserted-by":"crossref","first-page":"a028126","DOI":"10.1101\/cshperspect.a028126","article-title":"Radial spokes-A snapshot of the motility regulation, assembly, and evolution of cilia and flagella","volume":"9","author":"Zhu","year":"2017","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib41","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1479-5876-7-61","article-title":"Sperm protein 17 is expressed in the sperm fibrous sheath","volume":"7","author":"Chiriva-Internati","year":"2009","journal-title":"J.\u00a0Transl. Med."},{"key":"10.1016\/j.celrep.2022.111683_bib42","doi-asserted-by":"crossref","first-page":"e58","DOI":"10.1002\/cpim.58","article-title":"Proximity ligation assay (PLA)","volume":"123","author":"Alam","year":"2018","journal-title":"Curr. Protoc. Immunol."},{"key":"10.1016\/j.celrep.2022.111683_bib43","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.semcdb.2003.12.006","article-title":"Integral UBL domain proteins: a family of proteasome interacting proteins","volume":"15","author":"Hartmann-Petersen","year":"2004","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib44","doi-asserted-by":"crossref","first-page":"10644","DOI":"10.1073\/pnas.92.23.10644","article-title":"Target sequence recognition by the calmodulin superfamily: implications from light chain binding to the regulatory domain of scallop myosin","volume":"92","author":"Houdusse","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2022.111683_bib45","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0955-0674(92)90055-H","article-title":"Unconventional myosins","volume":"4","author":"Cheney","year":"1992","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib46","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/cm.10155","article-title":"The radial spokes and central apparatus: mechano-chemical transducers that regulate flagellar motility","volume":"57","author":"Smith","year":"2004","journal-title":"Cell Motil Cytoskeleton"},{"key":"10.1016\/j.celrep.2022.111683_bib47","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1083\/jcb.63.1.35","article-title":"The structural basis of ciliary bend formation. Radial spoke positional changes accompanying microtubule sliding","volume":"63","author":"Warner","year":"1974","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib48","doi-asserted-by":"crossref","first-page":"2626","DOI":"10.1091\/mbc.e06-02-0095","article-title":"Disruption of the A-kinase anchoring domain in flagellar radial spoke protein 3 results in unregulated axonemal cAMP-dependent protein kinase activity and abnormal flagellar motility","volume":"17","author":"Gaillard","year":"2006","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.celrep.2022.111683_bib49","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1083\/jcb.153.6.1315","article-title":"Localization of calmodulin and dynein light chain LC8 in flagellar radial spokes","volume":"153","author":"Yang","year":"2001","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib50","doi-asserted-by":"crossref","first-page":"18905","DOI":"10.1074\/jbc.M002134200","article-title":"Casein kinase I is anchored on axonemal doublet microtubules and regulates flagellar dynein phosphorylation and activity","volume":"275","author":"Yang","year":"2000","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2022.111683_bib51","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1128\/EC.3.1.72-81.2004","article-title":"Flagellar radial spoke protein 2 is a calmodulin binding protein required for motility in Chlamydomonas reinhardtii","volume":"3","author":"Yang","year":"2004","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.celrep.2022.111683_bib52","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1091\/mbc.e04-04-0352","article-title":"Flagellar radial spokes contain a Ca2+-stimulated nucleoside diphosphate kinase","volume":"15","author":"Patel-King","year":"2004","journal-title":"Mol. Biol. Cell"},{"issue":"Pt 1","key":"10.1016\/j.celrep.2022.111683_bib53","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1242\/jcs.113.1.91","article-title":"Protein phosphatases PP1 and PP2A are located in distinct positions in the Chlamydomonas flagellar axoneme","volume":"113","author":"Yang","year":"2000","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2022.111683_bib54","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1083\/jcb.153.2.443","article-title":"Flagellar radial spoke protein 3 is an A-kinase anchoring protein (AKAP)","volume":"153","author":"Gaillard","year":"2001","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib55","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1002\/cm.20520","article-title":"Sequential assembly of flagellar radial spokes","volume":"68","author":"Diener","year":"2011","journal-title":"Cytoskeleton (Hoboken)"},{"key":"10.1016\/j.celrep.2022.111683_bib56","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1083\/jcb.200308132","article-title":"Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body","volume":"164","author":"Qin","year":"2004","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.celrep.2022.111683_bib57","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1008378","article-title":"NME5 frameshift variant in Alaskan Malamutes with primary ciliary dyskinesia","volume":"15","author":"Anderegg","year":"2019","journal-title":"PLoS Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib58","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.ajhg.2019.12.010","article-title":"TTC12 loss-of-function mutations cause primary ciliary dyskinesia and unveil distinct dynein assembly mechanisms in motile cilia versus flagella","volume":"106","author":"Thomas","year":"2020","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib59","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s00439-020-02110-0","article-title":"Novel mutations in SPEF2 causing different defects between flagella and cilia bridge: the phenotypic link between MMAF and PCD","volume":"139","author":"Tu","year":"2020","journal-title":"Hum. Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib60","doi-asserted-by":"crossref","first-page":"1996","DOI":"10.1093\/hmg\/ddab171","article-title":"Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse","volume":"30","author":"Zhang","year":"2021","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.celrep.2022.111683_bib61","doi-asserted-by":"crossref","first-page":"jcs259381","DOI":"10.1242\/jcs.259381","article-title":"LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice","volume":"134","author":"Zhang","year":"2021","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2022.111683_bib62","doi-asserted-by":"crossref","first-page":"jcs221648","DOI":"10.1242\/jcs.221648","article-title":"RSPH6A is required for sperm flagellum formation and male fertility in mice","volume":"131","author":"Abbasi","year":"2018","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.celrep.2022.111683_bib63","doi-asserted-by":"crossref","DOI":"10.1098\/rstb.2019.0153","article-title":"Asymmetries in the cilia of Chlamydomonas","volume":"375","author":"Dutcher","year":"2020","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"10.1016\/j.celrep.2022.111683_bib64","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.celrep.2022.111683_bib65","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.celrep.2022.111683_bib66","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2022.111683_bib67","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gky427","article-title":"SWISS-MODEL: homology modelling of protein structures and complexes","volume":"46","author":"Waterhouse","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.celrep.2022.111683_bib68","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera--a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J.\u00a0Comput. Chem."},{"key":"10.1016\/j.celrep.2022.111683_bib69","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification","volume":"26","author":"Cox","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.celrep.2022.111683_bib70","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1093\/molehr\/gau014","article-title":"SHCBP1L, a conserved protein in mammals, is predominantly expressed in male germ cells and maintains spindle stability during meiosis in testis","volume":"20","author":"Liu","year":"2014","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.celrep.2022.111683_bib71","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1262\/jrd.2018-136","article-title":"Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis","volume":"65","author":"Shimada","year":"2019","journal-title":"J.\u00a0Reprod. Dev."},{"key":"10.1016\/j.celrep.2022.111683_bib72","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.15252\/embj.201386855","article-title":"Reduced pachytene piRNAs and translation underlie spermiogenic arrest in Maelstrom mutant mice","volume":"33","author":"Casta\u00f1eda","year":"2014","journal-title":"EMBO J."},{"key":"10.1016\/j.celrep.2022.111683_bib73","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1021\/acs.jproteome.9b00496","article-title":"LFQ-analyst: an easy-to-use interactive web platform to analyze and visualize label-free proteomics data preprocessed with MaxQuant","volume":"19","author":"Shah","year":"2020","journal-title":"J.\u00a0Proteome Res."},{"key":"10.1016\/j.celrep.2022.111683_bib74","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gky1106","article-title":"The PRIDE database and related tools and resources in 2019: improving support for quantification data","volume":"47","author":"Perez-Riverol","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.celrep.2022.111683_bib75","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1038\/nprot.2008.150","article-title":"The SCX\/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry","volume":"3","author":"Vill\u00e9n","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.celrep.2022.111683_bib76","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1074\/mcp.M114.045468","article-title":"Quantitative phosphoproteomics analysis reveals a key role of insulin growth factor 1 receptor (IGF1R) tyrosine kinase in human sperm capacitation","volume":"14","author":"Wang","year":"2015","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.celrep.2022.111683_bib77","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.jprot.2013.02.008","article-title":"Comparative evaluation of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and high-pH reversed phase (Hp-RP) chromatography in profiling of rat kidney proteome","volume":"82","author":"Hao","year":"2013","journal-title":"J.\u00a0Proteomics"},{"key":"10.1016\/j.celrep.2022.111683_bib78","doi-asserted-by":"crossref","first-page":"D447","DOI":"10.1093\/nar\/gkv1145","article-title":"2016 update of the PRIDE database and its related tools","volume":"44","author":"Vizca\u00edno","year":"2016","journal-title":"Nucleic Acids Res."}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124722015571?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124722015571?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T11:52:03Z","timestamp":1761911523000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124722015571"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11]]},"references-count":78,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["S2211124722015571"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2022.111683","relation":{},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2022,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2022.111683","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Author(s).","name":"copyright","label":"Copyright"}],"article-number":"111683"}}