{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T15:55:23Z","timestamp":1783785323615,"version":"3.55.0"},"reference-count":53,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T00:00:00Z","timestamp":1715040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI150961"],"award-info":[{"award-number":["R01AI150961"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005302","name":"University of Illinois Urbana-Champaign","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005302","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1016\/j.celrep.2024.114263","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T08:02:14Z","timestamp":1716969734000},"page":"114263","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"title":["The Cryptosporidium signaling kinase CDPK5 plays an important role in male gametogenesis and parasite virulence"],"prefix":"10.1016","volume":"43","author":[{"given":"Maria G.","family":"Nava","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Szewczyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justine V.","family":"Arrington","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tauqeer","family":"Alam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4549-6268","authenticated-orcid":false,"given":"Sumiti","family":"Vinayak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2024.114263_bib1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0140-6736(13)60844-2","article-title":"Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study","volume":"382","author":"Kotloff","year":"2013","journal-title":"Lancet"},{"key":"10.1016\/j.celrep.2024.114263_bib2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004729","article-title":"The Burden of Cryptosporidium Diarrheal Disease among Children\u00a0<\u00a024 Months of Age in Moderate\/High Mortality Regions of Sub-Saharan Africa and South Asia, Utilizing Data from the Global Enteric Multicenter Study (GEMS)","volume":"10","author":"Sow","year":"2016","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.celrep.2024.114263_bib3","doi-asserted-by":"crossref","first-page":"e568","DOI":"10.1016\/S2214-109X(19)30076-2","volume":"7","author":"Kotloff","year":"2019","journal-title":"Lancet Global Health"},{"key":"10.1016\/j.celrep.2024.114263_bib4","doi-asserted-by":"crossref","DOI":"10.1136\/bmjgh-2023-012540","article-title":"Safe and effective treatments are needed for cryptosporidiosis, a truly neglected tropical disease","volume":"8","author":"Gilbert","year":"2023","journal-title":"BMJ Glob. Health"},{"key":"10.1016\/j.celrep.2024.114263_bib5","doi-asserted-by":"crossref","first-page":"e758","DOI":"10.1016\/S2214-109X(18)30283-3","article-title":"Morbidity, mortality, and long-term consequences associated with diarrhoea from Cryptosporidium infection in children younger than 5 years: a meta-analyses study","volume":"6","author":"Khalil","year":"2018","journal-title":"Lancet Global Health"},{"key":"10.1016\/j.celrep.2024.114263_bib6","doi-asserted-by":"crossref","first-page":"e564","DOI":"10.1016\/S2214-109X(15)00151-5","article-title":"Pathogen-specific burdens of community diarrhoea in developing countries: a multisite birth cohort study (MAL-ED)","volume":"3","author":"Platts-Mills","year":"2015","journal-title":"Lancet Global Health"},{"key":"10.1016\/j.celrep.2024.114263_bib7","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.cvfa.2019.11.005","article-title":"Cryptosporidium and Giardia in Ruminants","volume":"36","author":"Santin","year":"2020","journal-title":"Vet. Clin. North Am. Food Anim. Pract."},{"key":"10.1016\/j.celrep.2024.114263_bib8","doi-asserted-by":"crossref","first-page":"568","DOI":"10.15585\/mmwr.mm6825a3","article-title":"Cryptosporidiosis Outbreaks - United States, 2009-2017","volume":"68","author":"Gharpure","year":"2019","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"10.1016\/j.celrep.2024.114263_bib9","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1186\/1471-2334-9-195","article-title":"High dose prolonged treatment with nitazoxanide is not effective for cryptosporidiosis in HIV positive Zambian children: a randomised controlled trial","volume":"9","author":"Amadi","year":"2009","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.celrep.2024.114263_bib10","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1016\/S0140-6736(02)11401-2","article-title":"Effect of nitazoxanide on morbidity and mortality in Zambian children with cryptosporidiosis: a randomised controlled trial","volume":"360","author":"Amadi","year":"2002","journal-title":"Lancet"},{"key":"10.1016\/j.celrep.2024.114263_bib11","doi-asserted-by":"crossref","first-page":"2226","DOI":"10.1038\/s41564-019-0539-x","article-title":"Life cycle progression and sexual development of the apicomplexan parasite Cryptosporidium parvum","volume":"4","author":"Tandel","year":"2019","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.celrep.2024.114263_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3001604","article-title":"Live imaging of the Cryptosporidium parvum life cycle reveals direct development of\u00a0male and female gametes from type I meronts","volume":"20","author":"English","year":"2022","journal-title":"PLoS Biol."},{"key":"10.1016\/j.celrep.2024.114263_bib13","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.pt.2019.11.003","article-title":"Cryptosporidium parvum","volume":"36","author":"Dumaine","year":"2020","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.celrep.2024.114263_bib14","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.03261-22","article-title":"Genetic Ablation of a Female-Specific Apetala 2 Transcription Factor Blocks Oocyst Shedding in Cryptosporidium parvum","volume":"14","author":"Tandel","year":"2023","journal-title":"mBio"},{"key":"10.1016\/j.celrep.2024.114263_bib15","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1007\/BF00931092","article-title":"Cryptosporidium sp. of the starred lizard Agama stellio: Ultrastructure and life cycle","volume":"76","author":"Ostrovska","year":"1990","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.celrep.2024.114263_bib16","first-page":"331","article-title":"Ultrastructure of microgametogenesis, microgametes and gametogony of Cryptosporidium sp. in the small intestine of mice","volume":"18","author":"G\u00f6ebel","year":"1982","journal-title":"PROTISTOLOGICA"},{"key":"10.1016\/j.celrep.2024.114263_bib17","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1098\/rspb.1976.0031","article-title":"Gametogenesis and fertilization in Plasmodium yoelii nigeriensis: a transmission electron microscope study","volume":"193","author":"Sinden","year":"1976","journal-title":"Proc. R. Soc. Lond. B Biol. Sci."},{"key":"10.1016\/j.celrep.2024.114263_bib18","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1010276","article-title":"Live-cell fluorescence imaging of microgametogenesis in the human malaria parasite Plasmodium falciparum","volume":"18","author":"Yahiya","year":"2022","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2024.114263_bib19","doi-asserted-by":"crossref","first-page":"016566-17","DOI":"10.1128\/mBio.01656-17","article-title":"Plasmodium falciparum calcium-dependent protein kinase 2 is critical for male gametocyte exflagellation but not essential for asexual proliferation","volume":"8","author":"Bansal","year":"2017","journal-title":"mBio"},{"key":"10.1016\/j.celrep.2024.114263_bib20","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0092-8674(04)00449-0","article-title":"Calcium and a calcium-dependent protein kinase regulate gamete formation and mosquito transmission in a malaria parasite","volume":"117","author":"Billker","year":"2004","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2024.114263_bib21","doi-asserted-by":"crossref","first-page":"001300","DOI":"10.1128\/mBio.00130-18","article-title":"Calcium-Dependent Protein Kinase 5 Is Required for Release of Egress-Specific Organelles in Plasmodium falciparum","volume":"9","author":"Absalon","year":"2018","journal-title":"mBio"},{"key":"10.1016\/j.celrep.2024.114263_bib22","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nature09022","article-title":"Calcium-dependent protein kinase 1 is an essential regulator of\u00a0exocytosis in Toxoplasma","volume":"465","author":"Lourido","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2024.114263_bib23","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1172\/JCI61822","article-title":"Transmission of malaria to mosquitoes blocked by bumped kinase inhibitors","volume":"122","author":"Ojo","year":"2012","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.celrep.2024.114263_bib24","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1126\/science.1188191","article-title":"A plant-like kinase in Plasmodium falciparum regulates parasite egress from erythrocytes","volume":"328","author":"Dvorin","year":"2010","journal-title":"Science"},{"key":"10.1016\/j.celrep.2024.114263_bib25","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.pt.2019.06.013","article-title":"The Symbiotic Spectrum: Where Do the Gregarines Fit?","volume":"35","author":"Rueckert","year":"2019","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.celrep.2024.114263_bib26","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1186\/s12864-022-08700-8","article-title":"Marine gregarine genomes reveal the breadth of apicomplexan diversity with a partially conserved glideosome machinery","volume":"23","author":"Boisard","year":"2022","journal-title":"BMC Genom."},{"key":"10.1016\/j.celrep.2024.114263_bib27","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01231-20","article-title":"A conditional protein degradation system to study essential gene function in Cryptosporidium parvum","volume":"11","author":"Choudhary","year":"2020","journal-title":"mBio"},{"key":"10.1016\/j.celrep.2024.114263_bib28","doi-asserted-by":"crossref","first-page":"D808","DOI":"10.1093\/nar\/gkad1003","article-title":"VEuPathDB: the eukaryotic pathogen, vector and host bioinformatics resource center in 2023","volume":"52","author":"Alvarez-Jarreta","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.celrep.2024.114263_bib29","doi-asserted-by":"crossref","first-page":"317155","DOI":"10.1371\/journal.pone.0031715","article-title":"The Cryptosporidium parvum transcriptome during in\u00a0vitro development","volume":"7","author":"Mauzy","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.celrep.2024.114263_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1008131","article-title":"Overlapping and distinct roles of CDPK family members in the pre-erythrocytic stages of the rodent malaria parasite, Plasmodium berghei","volume":"16","author":"Govindasamy","year":"2020","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2024.114263_bib31","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1073\/pnas.1715443115","article-title":"PfCDPK1 is critical for malaria parasite gametogenesis and mosquito infection","volume":"115","author":"Bansal","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.celrep.2024.114263_bib32","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1038\/nature14651","article-title":"Genetic modification of the diarrhoeal pathogen Cryptosporidium parvum","volume":"523","author":"Vinayak","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2024.114263_bib33","doi-asserted-by":"crossref","first-page":"001244","DOI":"10.1128\/mSphere.00124-18","article-title":"Monoclonal antibodies to intracellular stages of Cryptosporidium parvum define life cycle progression in\u00a0vitro","volume":"3","author":"Wilke","year":"2018","journal-title":"mSphere"},{"key":"10.1016\/j.celrep.2024.114263_bib34","first-page":"1","article-title":"Cryptosporidium rhoptry effector protein ROP1 injected during invasion targets the host cytoskeletal modulator LMO7","author":"Gu\u00e9rin","year":"2021","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.celrep.2024.114263_bib35","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1371\/journal.pbio.0060139","article-title":"Gametogenesis in malaria parasites is mediated by the cGMP-dependent protein kinase","volume":"6","author":"McRobert","year":"2008","journal-title":"PLoS Biol."},{"key":"10.1016\/j.celrep.2024.114263_bib36","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1021\/cb501004q","article-title":"Identification of Potent Phosphodiesterase Inhibitors that Demonstrate Cyclic Nucleotide-Dependent Functions in Apicomplexan Parasites","volume":"10","author":"Howard","year":"2015","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.celrep.2024.114263_bib37","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003344","article-title":"Malaria parasite cGMP-dependent protein kinase regulates blood stage merozoite secretory organelle discharge and egress","volume":"9","author":"Collins","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2024.114263_bib38","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abe5396","article-title":"Ca2+ signals critical for egress and gametogenesis in malaria parasites depend on a multipass membrane protein that interacts with PKG","volume":"7","author":"Balestra","year":"2021","journal-title":"Sci. Adv."},{"key":"10.1016\/j.celrep.2024.114263_bib39","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1010901","article-title":"A positive feedback loop mediates crosstalk between calcium, cyclic nucleotide and lipid signalling in calcium-induced Toxoplasma gondii egress","volume":"18","author":"Nofal","year":"2022","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.celrep.2024.114263_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.molbiopara.2020.111277","article-title":"Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An\u00a0essential mediator of merozoite egress","volume":"237","author":"Nava","year":"2020","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.celrep.2024.114263_bib41","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1038\/s41564-018-0177-8","article-title":"Modelling Cryptosporidium infection in human small intestinal and lung organoids","volume":"3","author":"Heo","year":"2018","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.celrep.2024.114263_bib42","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.chom.2019.05.007","article-title":"A stem-cell-derived platform enables complete Cryptosporidium development in-vitro and genetic tractability","volume":"26","author":"Wilke","year":"2019","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.celrep.2024.114263_bib43","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1046\/j.1365-2958.1999.01174.x","article-title":"Mobilization of intracellular calcium stimulates microneme discharge in Toxoplasma gondii","volume":"31","author":"Carruthers","year":"1999","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.celrep.2024.114263_bib44","doi-asserted-by":"crossref","first-page":"26914","DOI":"10.1074\/jbc.M115.652511","article-title":"Calcium Signaling throughout the Toxoplasma gondii lytic\u00a0cycle: A STUDY USING GENETICALLY ENCODED CALCIUM INDICATORS","volume":"290","author":"Borges-Pereira","year":"2015","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.celrep.2024.114263_bib45","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.celrep.2024.114263_bib46","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.celrep.2024.114263_bib47","doi-asserted-by":"crossref","DOI":"10.1002\/cpbi.102","article-title":"Using SPAdes De Novo Assembler","volume":"70","author":"Prjibelski","year":"2020","journal-title":"Curr Protoc Bioinform"},{"key":"10.1016\/j.celrep.2024.114263_bib48","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/978-1-4939-9748-0_13","article-title":"Genetic manipulation of Cryptosporidium parvum with CRISPR\/Cas9","volume":"2052","author":"Sateriale","year":"2020","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.celrep.2024.114263_bib49","doi-asserted-by":"crossref","first-page":"20B.2.1","DOI":"10.1002\/cpmc.33","article-title":"Generating and maintaining transgenic Cryptosporidium parvum parasites","volume":"46","author":"Pawlowic","year":"2017","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.celrep.2024.114263_bib50","series-title":"Cryptosporidium and Cryptosporidiosis","first-page":"181","author":"Upton","year":"1997"},{"key":"10.1016\/j.celrep.2024.114263_bib51","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1038\/nature22337","article-title":"A Cryptosporidium PI(4)K inhibitor is a drug candidate for cryptosporidiosis","volume":"546","author":"Manjunatha","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2024.114263_bib52","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1021\/ac4031175","article-title":"Sequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography","volume":"86","author":"Tsai","year":"2014","journal-title":"Anal. Chem."},{"key":"10.1016\/j.celrep.2024.114263_bib53","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1038\/nprot.2007.261","article-title":"Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips","volume":"2","author":"Rappsilber","year":"2007","journal-title":"Nat. Protoc."}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124724005916?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124724005916?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T12:36:29Z","timestamp":1779453389000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124724005916"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":53,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["S2211124724005916"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2024.114263","relation":{},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The Cryptosporidium signaling kinase CDPK5 plays an important role in male gametogenesis and parasite virulence","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2024.114263","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"114263"}}