{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:18:29Z","timestamp":1792289909012,"version":"4.4.0"},"reference-count":53,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,13]],"date-time":"2024-10-13T00:00:00Z","timestamp":1728777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01DK119210"],"award-info":[{"award-number":["R01DK119210"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["1F32DK131792"],"award-info":[{"award-number":["1F32DK131792"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R21HD106036"],"award-info":[{"award-number":["R21HD106036"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2024,11]]},"DOI":"10.1016\/j.celrep.2024.114919","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T16:40:45Z","timestamp":1730306445000},"page":"114919","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":18,"title":["Highly neurogenic glia from human and mouse myenteric ganglia generate functional neurons following culture and transplantation into the gut"],"prefix":"10.1016","volume":"43","author":[{"given":"Jessica L.","family":"Mueller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail R.","family":"Leavitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher Y.","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah C.","family":"Ott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narges S.","family":"Mahdavian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona E.","family":"Carbone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian K.","family":"King","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan J.","family":"Burns","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Poole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Hotta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan M.","family":"Goldstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhian","family":"Stavely","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2024.114919_bib1","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1038\/nrgastro.2012.32","article-title":"The enteric nervous system and neurogastroenterology","volume":"9","author":"Furness","year":"2012","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.celrep.2024.114919_bib2","series-title":"The Enteric Nervous System","author":"Furness","year":"2006"},{"key":"10.1016\/j.celrep.2024.114919_bib3","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.ydbio.2016.03.032","article-title":"Clinical aspects of neurointestinal disease: pathophysiology, diagnosis, and treatment","volume":"417","author":"Goldstein","year":"2016","journal-title":"Dev. Biol."},{"key":"10.1016\/j.celrep.2024.114919_bib4","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.semcdb.2017.01.006","article-title":"Enteric nervous system development: a crest cell\u2019s journey from neural tube to colon","volume":"66","author":"Nagy","year":"2017","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.celrep.2024.114919_bib5","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/cge.12054","article-title":"Building a brain in the gut: development of the enteric nervous system","volume":"83","author":"Goldstein","year":"2013","journal-title":"Clin. Genet."},{"key":"10.1016\/j.celrep.2024.114919_bib6","doi-asserted-by":"crossref","first-page":"3398","DOI":"10.1172\/JCI58186","article-title":"Enteric glia are multipotent in culture but primarily form glia in the adult rodent gut","volume":"121","author":"Joseph","year":"2011","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.celrep.2024.114919_bib7","doi-asserted-by":"crossref","first-page":"G274","DOI":"10.1152\/ajpgi.00371.2016","article-title":"Enteric glia cells are critical to limiting the intestinal inflammatory response after injury","volume":"312","author":"Langness","year":"2017","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"10.1016\/j.celrep.2024.114919_bib8","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1136\/gut.2006.094565","article-title":"Characterisation and transplantation of enteric nervous system progenitor cells","volume":"56","author":"Almond","year":"2007","journal-title":"Gut"},{"key":"10.1016\/j.celrep.2024.114919_bib9","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1007\/s00384-005-0051-z","article-title":"Isolation and cultivation of neuronal precursor cells from the developing human enteric nervous system as a tool for cell therapy in dysganglionosis","volume":"21","author":"Rauch","year":"2006","journal-title":"Int. J. Colorectal Dis."},{"key":"10.1016\/j.celrep.2024.114919_bib10","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1053\/j.gastro.2008.03.035","article-title":"Human and mouse enteric nervous system neurosphere transplants regulate the function of aganglionic embryonic distal colon","volume":"135","author":"Lindley","year":"2008","journal-title":"Gastroenterology"},{"key":"10.1016\/j.celrep.2024.114919_bib11","doi-asserted-by":"crossref","first-page":"2063","DOI":"10.1053\/j.gastro.2009.06.038","article-title":"Expansion and differentiation of neural progenitors derived from the human adult enteric nervous system","volume":"137","author":"Metzger","year":"2009","journal-title":"Gastroenterology"},{"key":"10.1016\/j.celrep.2024.114919_bib12","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.jss.2016.08.035","article-title":"Optimizing neurogenic potential of enteric neurospheres for treatment of neurointestinal diseases","volume":"206","author":"Cheng","year":"2016","journal-title":"J.\u00a0Surg. Res."},{"key":"10.1016\/j.celrep.2024.114919_bib13","doi-asserted-by":"crossref","first-page":"2214","DOI":"10.1053\/j.gastro.2009.02.048","article-title":"Enteric nervous system stem cells derived from human gut mucosa for the treatment of aganglionic gut disorders","volume":"136","author":"Metzger","year":"2009","journal-title":"Gastroenterology"},{"key":"10.1016\/j.celrep.2024.114919_bib14","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1038\/pr.2017.4","article-title":"Postnatal human enteric neuronal progenitors can migrate, differentiate, and proliferate in embryonic and postnatal aganglionic gut environments","volume":"81","author":"Cheng","year":"2017","journal-title":"Pediatr. Res."},{"key":"10.1016\/j.celrep.2024.114919_bib15","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1002\/stem.3388","article-title":"Enteric mesenchymal cells support the growth of postnatal enteric neural stem cells","volume":"39","author":"Stavely","year":"2021","journal-title":"Stem Cell."},{"key":"10.1016\/j.celrep.2024.114919_bib16","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.jpedsurg.2009.02.048","article-title":"Properties of secondary and tertiary human enteric nervous system neurospheres","volume":"44","author":"Lindley","year":"2009","journal-title":"J.\u00a0Pediatr. Surg."},{"key":"10.1016\/j.celrep.2024.114919_bib17","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1172\/JCI65963","article-title":"Transplanted progenitors generate functional enteric neurons in the postnatal colon","volume":"123","author":"Hotta","year":"2013","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.celrep.2024.114919_bib18","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0093605","article-title":"In\u00a0vivo transplantation of neurosphere-like bodies derived from the human postnatal and adult enteric nervous system: a pilot study","volume":"9","author":"Hetz","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.celrep.2024.114919_bib19","doi-asserted-by":"crossref","first-page":"6387","DOI":"10.1242\/dev.00857","article-title":"Neuron and glia generating progenitors of the mammalian enteric nervous system isolated from foetal and postnatal gut cultures","volume":"130","author":"Bondurand","year":"2003","journal-title":"Development"},{"key":"10.1016\/j.celrep.2024.114919_bib20","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.ydbio.2016.04.001","article-title":"White paper on guidelines concerning enteric nervous system stem cell therapy for enteric neuropathies","volume":"417","author":"Burns","year":"2016","journal-title":"Dev. Biol."},{"key":"10.1016\/j.celrep.2024.114919_bib21","doi-asserted-by":"crossref","first-page":"3412","DOI":"10.1172\/JCI58200","article-title":"Glial cells in the mouse enteric nervous system can undergo neurogenesis in response to injury","volume":"121","author":"Laranjeira","year":"2011","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.celrep.2024.114919_bib22","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1038\/s41598-017-02890-y","article-title":"Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells","volume":"7","author":"Belkind-Gerson","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.celrep.2024.114919_bib23","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1097\/MIB.0000000000000326","article-title":"Colitis induces enteric neurogenesis through a 5-HT4-dependent mechanism","volume":"21","author":"Belkind-Gerson","year":"2015","journal-title":"Inflamm. Bowel Dis."},{"key":"10.1016\/j.celrep.2024.114919_bib24","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1002\/glia.22746","article-title":"Heterogeneity and phenotypic plasticity of glial cells in the mammalian enteric nervous system","volume":"63","author":"Boesmans","year":"2015","journal-title":"Glia"},{"key":"10.1016\/j.celrep.2024.114919_bib25","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.775102","article-title":"Development, diversity, and neurogenic capacity of enteric glia","volume":"9","author":"Boesmans","year":"2022","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.celrep.2024.114919_bib26","doi-asserted-by":"crossref","first-page":"2040","DOI":"10.1002\/glia.22876","article-title":"Enteric glia express proteolipid protein 1 and are a transcriptionally unique population of glia in the mammalian nervous system","volume":"63","author":"Rao","year":"2015","journal-title":"Glia"},{"key":"10.1016\/j.celrep.2024.114919_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.112194","article-title":"Single-cell multiome sequencing clarifies enteric glial diversity and identifies an intraganglionic population poised for neurogenesis","volume":"42","author":"Guyer","year":"2023","journal-title":"Cell Rep."},{"key":"10.1016\/j.celrep.2024.114919_bib28","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.neuron.2011.05.001","article-title":"Adult neurogenesis in the mammalian brain: significant answers and significant questions","volume":"70","author":"Ming","year":"2011","journal-title":"Neuron"},{"key":"10.1016\/j.celrep.2024.114919_bib29","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1523\/JNEUROSCI.22-03-00876.2002","article-title":"Proteolipid promoter activity distinguishes two populations of NG2-positive cells throughout neonatal cortical development","volume":"22","author":"Mallon","year":"2002","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.celrep.2024.114919_bib30","article-title":"The-Mouse-in-Biomedical-Research, 2nd Ed","volume":"Volume 3","author":"Flurkey","year":"2007"},{"key":"10.1016\/j.celrep.2024.114919_bib31","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1002\/dvg.22866","article-title":"Generation of BAF53b-Cre transgenic mice with pan-neuronal Cre activities","volume":"53","author":"Zhan","year":"2015","journal-title":"Genesis"},{"key":"10.1016\/j.celrep.2024.114919_bib32","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/nn.3078","article-title":"A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing","volume":"15","author":"Madisen","year":"2012","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.celrep.2024.114919_bib33","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1038\/s41586-021-03852-1","article-title":"Cells of the human intestinal tract mapped across space and time","volume":"597","author":"Elmentaite","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2024.114919_bib34","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1016\/j.cell.2020.08.003","article-title":"The Human and Mouse Enteric Nervous System at Single-Cell Resolution","volume":"182","author":"Drokhlyansky","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2024.114919_bib35","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1126\/science.aam7511","article-title":"Lineage-dependent spatial and functional organization of the mammalian enteric nervous system","volume":"356","author":"Lasrado","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.celrep.2024.114919_bib36","doi-asserted-by":"crossref","first-page":"5904","DOI":"10.1038\/s41467-023-41492-3","article-title":"A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia","volume":"14","author":"Laddach","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.celrep.2024.114919_bib37","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1053\/j.gastro.2005.08.055","article-title":"Neural stem cell transplantation in the stomach rescues gastric function in neuronal nitric oxide synthase-deficient mice","volume":"129","author":"Micci","year":"2005","journal-title":"Gastroenterology"},{"key":"10.1016\/j.celrep.2024.114919_bib38","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1111\/nmo.12744","article-title":"Isogenic enteric neural progenitor cells can replace missing neurons and glia in mice with Hirschsprung disease","volume":"28","author":"Hotta","year":"2016","journal-title":"Neuro Gastroenterol. Motil."},{"key":"10.1016\/j.celrep.2024.114919_bib39","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/nature16951","article-title":"Deriving human ENS lineages for cell therapy and drug discovery in Hirschsprung disease","volume":"531","author":"Fattahi","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2024.114919_bib40","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.1016\/j.neuron.2024.06.018","article-title":"Mature enteric neurons have the capacity to reinnervate the intestine with glial cells as their guide","volume":"112","author":"Stavely","year":"2024","journal-title":"Neuron"},{"key":"10.1016\/j.celrep.2024.114919_bib41","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/j.jcmgh.2024.01.012","article-title":"Optogenetic activation of cholinergic enteric neurons reduces inflammation in experimental colitis","volume":"17","author":"Rahman","year":"2024","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"10.1016\/j.celrep.2024.114919_bib42","doi-asserted-by":"crossref","first-page":"eabl8753","DOI":"10.1126\/scitranslmed.abl8753","article-title":"Schwann cells in the subcutaneous adipose tissue have neurogenic potential and can be used for regenerative therapies","volume":"14","author":"Stavely","year":"2022","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.celrep.2024.114919_bib43","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.celrep.2024.114919_bib44","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1093\/stcltm\/szae013","article-title":"Agrin inhibition in enteric neural stem cells enhances their migration following colonic transplantation","volume":"13","author":"Mueller","year":"2024","journal-title":"Stem Cells Transl. Med."},{"key":"10.1016\/j.celrep.2024.114919_bib45","doi-asserted-by":"crossref","DOI":"10.1177\/09636897231215233","article-title":"Isolation, expansion, and endoscopic delivery of autologous enteric neuronal stem cells in swine","volume":"32","author":"Hotta","year":"2023","journal-title":"Cell Transplant."},{"key":"10.1016\/j.celrep.2024.114919_bib46","doi-asserted-by":"crossref","first-page":"5211","DOI":"10.3390\/ijms24065211","article-title":"Bone marrow stem cells derived from nerves have neurogenic properties and potential utility for regenerative therapy","volume":"24","author":"Ott","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.celrep.2024.114919_bib47","article-title":"In situ Ca2+ imaging of the enteric nervous system","volume":"95","author":"Fried","year":"2015","journal-title":"JoVE"},{"key":"10.1016\/j.celrep.2024.114919_bib48","doi-asserted-by":"crossref","DOI":"10.1002\/imt2.43","article-title":"Complex heatmap visualization","volume":"1","author":"Gu","year":"2022","journal-title":"iMeta"},{"key":"10.1016\/j.celrep.2024.114919_bib49","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1038\/nn1525","article-title":"Millisecond-timescale, genetically targeted optical control of neural activity","volume":"8","author":"Boyden","year":"2005","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.celrep.2024.114919_bib50","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms14049","article-title":"Massively parallel digital transcriptional profiling of single cells","volume":"8","author":"Zheng","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.celrep.2024.114919_bib51","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1016\/j.cell.2019.05.031","article-title":"Comprehensive integration of single-cell data","volume":"177","author":"Stuart","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2024.114919_bib52","doi-asserted-by":"crossref","DOI":"10.1093\/gigascience\/giaa151","article-title":"SoupX removes ambient RNA contamination from droplet-based single-cell RNA sequencing data","volume":"9","author":"Young","year":"2020","journal-title":"GigaScience"},{"key":"10.1016\/j.celrep.2024.114919_bib53","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cels.2019.03.003","article-title":"DoubletFinder: Doublet Detection in Single-Cell RNA Sequencing Data Using Artificial Nearest Neighbors","volume":"8","author":"McGinnis","year":"2019","journal-title":"Cell Syst."}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124724012701?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124724012701?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T13:44:24Z","timestamp":1773236664000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124724012701"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":53,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["S2211124724012701"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2024.114919","relation":{},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Highly neurogenic glia from human and mouse myenteric ganglia generate functional neurons following culture and transplantation into the gut","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2024.114919","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"114919"}}