{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:55:16Z","timestamp":1791230116899,"version":"4.1.0"},"reference-count":47,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["202269\/A\/16\/Z"],"award-info":[{"award-number":["202269\/A\/16\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["803852"],"award-info":[{"award-number":["803852"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017540","name":"National Heart Lung and Blood Institute Division of Intramural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017540","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.celrep.2025.116221","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T16:12:59Z","timestamp":1757002379000},"page":"116221","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"title":["Replication competition drives the selective mtDNA inheritance in Drosophila ovary"],"prefix":"10.1016","volume":"44","author":[{"given":"Cheng","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hansong","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1423-1809","authenticated-orcid":false,"given":"Hong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.celrep.2025.116221_bib1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/nrg1606","article-title":"Mitochondrial DNA mutations in human disease","volume":"6","author":"Taylor","year":"2005","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.celrep.2025.116221_bib2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.0060035","article-title":"Mitigating mutational meltdown in mammalian mitochondria","volume":"6","author":"Rand","year":"2008","journal-title":"PLoS Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib3","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1534\/genetics.104.91769","article-title":"Mitochondria as Chi","volume":"179","author":"Wallace","year":"2008","journal-title":"Genetics"},{"key":"10.1016\/j.celrep.2025.116221_bib4","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1038\/ng1096-146","article-title":"Random genetic drift in the female germline explains the rapid segregation of mammalian mitochondrial DNA","volume":"14","author":"Jenuth","year":"1996","journal-title":"Nat. Genet."},{"key":"10.1016\/j.celrep.2025.116221_bib5","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/ng.2007.63","article-title":"A reduction of mitochondrial DNA molecules during embryogenesis explains the rapid segregation of genotypes","volume":"40","author":"Cree","year":"2008","journal-title":"Nat. Genet."},{"key":"10.1016\/j.celrep.2025.116221_bib6","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/PL00006487","article-title":"Nucleotide substitution rate of mammalian mitochondrial genomes","volume":"48","author":"Pesole","year":"1999","journal-title":"J. Mol. Evol."},{"key":"10.1016\/j.celrep.2025.116221_bib7","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1126\/science.1147786","article-title":"A mouse model of mitochondrial disease reveals germline selection against severe mtDNA mutations","volume":"319","author":"Fan","year":"2008","journal-title":"Science"},{"key":"10.1016\/j.celrep.2025.116221_bib8","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/ng.2920","article-title":"Selective propagation of functional mitochondrial DNA during oogenesis restricts the transmission of a deleterious mitochondrial variant","volume":"46","author":"Hill","year":"2014","journal-title":"Nat. Genet."},{"key":"10.1016\/j.celrep.2025.116221_bib9","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1038\/ng.2919","article-title":"Transmission of mitochondrial mutations and action of purifying selection in","volume":"46","author":"Ma","year":"2014","journal-title":"Nat. Genet."},{"key":"10.1016\/j.celrep.2025.116221_bib10","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1038\/s41586-019-1213-4","article-title":"Mitochondrial fragmentation drives selective removal of deleterious mtDNA in the germline","volume":"570","author":"Lieber","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.celrep.2025.116221_bib11","doi-asserted-by":"crossref","DOI":"10.1126\/science.aau6520","article-title":"Germline selection shapes human mitochondrial DNA diversity","volume":"364","author":"Wei","year":"2019","journal-title":"Science"},{"key":"10.1016\/j.celrep.2025.116221_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.0060010","article-title":"Strong purifying selection in transmission of mammalian mitochondrial DNA","volume":"6","author":"Stewart","year":"2008","journal-title":"PLoS Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib13","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1038\/s41556-017-0017-8","article-title":"Segregation of mitochondrial DNA heteroplasmy through a developmental genetic bottleneck in human embryos","volume":"20","author":"Floros","year":"2018","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib14","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1038\/s43587-024-00672-6","article-title":"Maternal age enhances purifying selection on pathogenic mutations in complex I genes of mammalian mtDNA","volume":"4","author":"Ru","year":"2024","journal-title":"Nat. Aging"},{"key":"10.1016\/j.celrep.2025.116221_bib15","doi-asserted-by":"crossref","first-page":"4330","DOI":"10.1016\/j.cub.2019.10.060","article-title":"A Genome-wide Screen Reveals that Reducing Mitochondrial DNA Polymerase Can Promote Elimination of Deleterious Mitochondrial Mutations","volume":"29","author":"Chiang","year":"2019","journal-title":"Curr. Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib16","doi-asserted-by":"crossref","DOI":"10.1083\/jcb.201905160","article-title":"Mitochondrial DNA segregation and replication restrict the transmission of detrimental mutation","volume":"219","author":"Chen","year":"2020","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib17","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1016\/j.cmet.2022.10.005","article-title":"Mitochondrial DNA quality control in the female germline requires a unique programmed mitophagy","volume":"34","author":"Palozzi","year":"2022","journal-title":"Cell Metab."},{"key":"10.1016\/j.celrep.2025.116221_bib18","doi-asserted-by":"crossref","first-page":"R1082","DOI":"10.1016\/j.cub.2008.09.011","article-title":"Drosophila oogenesis","volume":"18","author":"Bastock","year":"2008","journal-title":"Curr. Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib19","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1146\/annurev.genet.31.1.405","article-title":"Germline cyst formation in Drosophila","volume":"31","author":"de Cuevas","year":"1997","journal-title":"Annu. Rev. Genet."},{"key":"10.1016\/j.celrep.2025.116221_bib20","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3389\/fcell.2020.00019","article-title":"Coordinating Proliferation, Polarity, and Cell Fate in the Female Germline","volume":"8","author":"Hinnant","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib21","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/0092-8674(93)90393-5","article-title":"Germline Cysts - Communes That Work","volume":"72","author":"Spradling","year":"1993","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2025.116221_bib22","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.49309","article-title":"Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila","volume":"8","author":"Wang","year":"2019","journal-title":"eLife"},{"key":"10.1016\/j.celrep.2025.116221_bib23","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1038\/s41556-022-00887-y","article-title":"The PPR domain of mitochondrial RNA polymerase is an exoribonuclease required for mtDNA replication in","volume":"24","author":"Liu","year":"2022","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib24","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1016\/j.devcel.2023.08.007","article-title":"Mechanical stimulation from the surrounding tissue activates mitochondrial energy metabolism in differentiating germ cells","volume":"58","author":"Wang","year":"2023","journal-title":"Dev. Cell"},{"key":"10.1016\/j.celrep.2025.116221_bib25","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/j.molcel.2019.01.013","article-title":"PINK1 Inhibits Local Protein Synthesis to Limit Transmission of Deleterious Mitochondrial DNA Mutations","volume":"73","author":"Zhang","year":"2019","journal-title":"Mol. Cell"},{"key":"10.1016\/j.celrep.2025.116221_bib26","first-page":"1466","article-title":"Manipulating the metazoan mitochondrial genome with targeted restriction enzymes (vol 321, pg 575, 2008)","volume":"322","author":"Xu","year":"2008","journal-title":"Science"},{"key":"10.1016\/j.celrep.2025.116221_bib27","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.07247","article-title":"Selections that isolate recombinant mitochondrial genomes in animals","volume":"4","author":"Ma","year":"2015","journal-title":"eLife"},{"key":"10.1016\/j.celrep.2025.116221_bib28","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1007\/BF02099755","article-title":"The mitochondrial DNA molecular of Drosophila yakuba: nucleotide sequence, gene organization, and genetic code","volume":"22","author":"Clary","year":"1985","journal-title":"J. Mol. Evol."},{"key":"10.1016\/j.celrep.2025.116221_bib29","article-title":"Single-molecule mitochondrial DNA imaging reveals heteroplasmy dynamics shaped by developmental bottlenecks and selection in different organs in vivo","author":"Chandrasegaram","year":"2025","journal-title":"bioRxiv"},{"key":"10.1016\/j.celrep.2025.116221_bib30","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1038\/ng.3587","article-title":"Selfish drive can trump function when animal mitochondrial genomes compete","volume":"48","author":"Ma","year":"2016","journal-title":"Nat. Genet."},{"key":"10.1016\/j.celrep.2025.116221_bib31","doi-asserted-by":"crossref","first-page":"11293","DOI":"10.1021\/jacs.8b05309","article-title":"Direct Visualization of Single-Nucleotide Variation in mtDNA Using a CRISPR\/Cas9-Mediated Proximity Ligation Assay","volume":"140","author":"Zhang","year":"2018","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.celrep.2025.116221_bib32","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nmeth723","article-title":"genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes","volume":"1","author":"Larsson","year":"2004","journal-title":"Nat. Methods"},{"key":"10.1016\/j.celrep.2025.116221_bib33","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.96536.3","article-title":"A transcription network underlies the dual genomic coordination of mitochondrial biogenesis","volume":"13","author":"Zhang","year":"2024","journal-title":"eLife"},{"key":"10.1016\/j.celrep.2025.116221_bib34","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1038\/s41594-024-01225-6","article-title":"Single-nucleoid architecture reveals heterogeneous packaging of mitochondrial DNA","volume":"31","author":"Isaac","year":"2024","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib35","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1083\/jcb.200712101","article-title":"Mitochondrial nucleoids maintain genetic autonomy but allow for functional complementation","volume":"181","author":"Gilkerson","year":"2008","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib36","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1091\/mbc.E14-11-1513","article-title":"Genetic mosaic analysis of a deleterious mitochondrial DNA mutation in Drosophila reveals novel aspects of mitochondrial regulation and function","volume":"26","author":"Chen","year":"2015","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.celrep.2025.116221_bib37","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/978-1-0716-2970-3_3","article-title":"Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries","volume":"2626","author":"Merkle","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib38","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0022-2836(78)90269-3","article-title":"Mechanism of mitochondrial DNA replication in mouse L-cells: kinetics of synthesis and turnover of the initiation sequence","volume":"119","author":"Bogenhagen","year":"1978","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.celrep.2025.116221_bib39","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1093\/oxfordjournals.jhered.a111480","article-title":"Relaxed and Stringent Genomes - Why Cytoplasmic Genes Dont Obey Mendels Laws","volume":"85","author":"Birky","year":"1994","journal-title":"J. Hered."},{"key":"10.1016\/j.celrep.2025.116221_bib40","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s00441-013-1628-4","article-title":"Mitochondrial DNA content of mature spermatozoa and oocytes in the genetic model Drosophila","volume":"353","author":"Wolff","year":"2013","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.celrep.2025.116221_bib41","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1038\/s44318-025-00377-5","article-title":"Mitochondrial DNA removal is essential for sperm development and activity","volume":"44","author":"Chen","year":"2025","journal-title":"EMBO J."},{"key":"10.1016\/j.celrep.2025.116221_bib42","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.15252\/embj.201592994","article-title":"The mitochondrial outer membrane protein MDI promotes local protein synthesis and mtDNA replication","volume":"35","author":"Zhang","year":"2016","journal-title":"EMBO J."},{"key":"10.1016\/j.celrep.2025.116221_bib43","doi-asserted-by":"crossref","first-page":"3904","DOI":"10.1016\/j.cell.2024.05.022","article-title":"The rate and nature of mitochondrial DNA mutations in human pedigrees","volume":"187","author":"Arnadottir","year":"2024","journal-title":"Cell"},{"key":"10.1016\/j.celrep.2025.116221_bib44","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/j.bbabio.2007.05.005","article-title":"Biochemical phenotypes associated with the mitochondrial ATP6 gene mutations at nt8993","volume":"1767","author":"Baracca","year":"2007","journal-title":"Bba-Bioenergetics"},{"key":"10.1016\/j.celrep.2025.116221_bib45","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1042\/BJ20051748","article-title":"Inefficient coupling between proton transport and ATP synthesis may be the pathogenic mechanism for NARP and Leigh syndrome resulting from the T8993G mutation in mtDNA","volume":"395","author":"Sgarbi","year":"2006","journal-title":"Biochem. J."},{"key":"10.1016\/j.celrep.2025.116221_bib46","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.cmet.2016.06.008","article-title":"Homeostatic Responses Regulate Selfish Mitochondrial Genome Dynamics in Caenorhabditis elegans","volume":"24","author":"Gitschlag","year":"2016","journal-title":"Cell Metab."},{"key":"10.1016\/j.celrep.2025.116221_bib47","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/s41556-021-00840-5","article-title":"LONP-1 and ATFS-1 sustain deleterious heteroplasmy by promoting mtDNA replication in dysfunctional mitochondria","volume":"24","author":"Yang","year":"2022","journal-title":"Nat. Cell Biol."}],"container-title":["Cell Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124725009921?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211124725009921?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:44:14Z","timestamp":1790138654000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211124725009921"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":47,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["S2211124725009921"],"URL":"https:\/\/doi.org\/10.1016\/j.celrep.2025.116221","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.04.29.650984","asserted-by":"object"}]},"ISSN":["2211-1247"],"issn-type":[{"value":"2211-1247","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Replication competition drives the selective mtDNA inheritance in Drosophila ovary","name":"articletitle","label":"Article Title"},{"value":"Cell Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.celrep.2025.116221","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"116221"}}