{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:19:20Z","timestamp":1791314360375,"version":"4.3.1"},"reference-count":39,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2013,7,17]],"date-time":"2013-07-17T00:00:00Z","timestamp":1374019200000},"content-version":"vor","delay-in-days":532,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["215618"],"award-info":[{"award-number":["215618"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005725","name":"CHDI Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biology"],"published-print":{"date-parts":[[2012,2]]},"DOI":"10.1016\/j.chembiol.2011.12.020","type":"journal-article","created":{"date-parts":[[2012,2,23]],"date-time":"2012-02-23T14:37:51Z","timestamp":1330007871000},"page":"264-275","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":77,"title":["TR-FRET-Based Duplex Immunoassay Reveals an Inverse Correlation of Soluble and Aggregated Mutant huntingtin in Huntington's Disease"],"prefix":"10.1016","volume":"19","author":[{"given":"Barbara","family":"Baldo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Paganetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Grueninger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Marcellin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda\u00a0S.","family":"Kaltenbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald\u00a0C.","family":"Lo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Semmelroth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andjelija","family":"Zivanovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doroth\u00e9e","family":"Abramowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregor\u00a0P.","family":"Lotz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian\u00a0P.","family":"Bates","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chembiol.2011.12.020_bib1","doi-asserted-by":"crossref","first-page":"7391","DOI":"10.1021\/bi011772q","article-title":"Amyloid-like features of polyglutamine aggregates and their assembly kinetics","volume":"41","author":"Chen","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.chembiol.2011.12.020_bib2","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.1126\/science.277.5334.1990","article-title":"Aggregation of huntingtin in neuronal intranuclear inclusions and dystrophic neurites in brain","volume":"277","author":"DiFiglia","year":"1997","journal-title":"Science"},{"key":"10.1016\/j.chembiol.2011.12.020_bib3","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1096\/fj.03-1449com","article-title":"Detection of oligomeric forms of alpha-synuclein protein in human plasma as a potential biomarker for Parkinson's disease","volume":"20","author":"El-Agnaf","year":"2006","journal-title":"FASEB J."},{"key":"10.1016\/j.chembiol.2011.12.020_bib4","doi-asserted-by":"crossref","first-page":"2716","DOI":"10.1096\/fj.09-150359","article-title":"High-molecular-weight beta-amyloid oligomers are elevated in cerebrospinal fluid of Alzheimer patients","volume":"24","author":"Fukumoto","year":"2010","journal-title":"FASEB J."},{"key":"10.1016\/j.chembiol.2011.12.020_bib5","doi-asserted-by":"crossref","first-page":"2522","DOI":"10.1523\/JNEUROSCI.19-07-02522.1999","article-title":"Nuclear and neuropil aggregates in Huntington's disease: relationship to neuropathology","volume":"19","author":"Gutekunst","year":"1999","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.chembiol.2011.12.020_bib6","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1006\/bbrc.1999.0337","article-title":"Rapid aggregate formation of the huntingtin N-terminal fragment carrying an expanded polyglutamine tract","volume":"256","author":"Hazeki","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.chembiol.2011.12.020_bib7","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1006\/bbrc.2000.3682","article-title":"Formic acid dissolves aggregates of an N-terminal huntingtin fragment containing an expanded polyglutamine tract: applying to quantification of protein components of the aggregates","volume":"277","author":"Hazeki","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.chembiol.2011.12.020_bib8","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/S0006-291X(02)00498-9","article-title":"Ultrastructure of nuclear aggregates formed by expressing an expanded polyglutamine","volume":"294","author":"Hazeki","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.chembiol.2011.12.020_bib9","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/j.1471-4159.2005.03348.x","article-title":"Purification of neuronal inclusions of patients with Huntington's disease reveals a broad range of N-terminal fragments of expanded huntingtin and insoluble polymers","volume":"95","author":"Hoffner","year":"2005","journal-title":"J.\u00a0Neurochem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib10","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1177\/1087057110373392","article-title":"Composite primary neuronal high-content screening assay for Huntington's disease incorporating non-cell-autonomous interactions","volume":"15","author":"Kaltenbach","year":"2010","journal-title":"J.\u00a0Biomol. Screen."},{"key":"10.1016\/j.chembiol.2011.12.020_bib11","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S0361-9230(01)00599-8","article-title":"New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins","volume":"56","author":"Ko","year":"2001","journal-title":"Brain Res. Bull."},{"key":"10.1016\/j.chembiol.2011.12.020_bib12","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1002\/1531-8249(199912)46:6<842::AID-ANA6>3.0.CO;2-O","article-title":"Huntington aggregates may not predict neuronal death in Huntington's disease","volume":"46","author":"Kuemmerle","year":"1999","journal-title":"Ann. Neurol."},{"key":"10.1016\/j.chembiol.2011.12.020_bib13","doi-asserted-by":"crossref","first-page":"8808","DOI":"10.1074\/jbc.M109.075028","article-title":"Proteolysis of mutant huntingtin produces an exon 1 fragment that accumulates as an aggregated protein in neuronal nuclei in Huntington disease","volume":"285","author":"Landles","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib14","doi-asserted-by":"crossref","first-page":"14777","DOI":"10.1074\/jbc.M109.093708","article-title":"Mutant huntingtin fragments form oligomers in a polyglutamine length-dependent manner in\u00a0vitro and in\u00a0vivo","volume":"285","author":"Legleiter","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib15","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1093\/hmg\/10.2.137","article-title":"Neurological abnormalities in a knock-in mouse model of Huntington's disease","volume":"10","author":"Lin","year":"2001","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.chembiol.2011.12.020_bib16","doi-asserted-by":"crossref","first-page":"38183","DOI":"10.1074\/jbc.M110.160218","article-title":"Hsp70 and Hsp40 functionally interact with soluble mutant huntingtin oligomers in a classic ATP-dependent reaction cycle","volume":"285","author":"Lotz","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib17","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S1097-2765(02)00602-0","article-title":"Proteases acting on mutant huntingtin generate cleaved products that differentially build up cytoplasmic and nuclear inclusions","volume":"10","author":"Lunkes","year":"2002","journal-title":"Mol. Cell"},{"key":"10.1016\/j.chembiol.2011.12.020_bib18","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1097\/00005072-199902000-00003","article-title":"Distribution of inclusions in neuronal nuclei and dystrophic neurites in Huntington disease brain","volume":"58","author":"Maat-Schieman","year":"1999","journal-title":"J.\u00a0Neuropathol. Exp. Neurol."},{"key":"10.1016\/j.chembiol.2011.12.020_bib19","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/S0092-8674(00)81369-0","article-title":"Exon 1 of the HD gene with an expanded CAG repeat is sufficient to cause a progressive neurological phenotype in transgenic mice","volume":"87","author":"Mangiarini","year":"1996","journal-title":"Cell"},{"key":"10.1016\/j.chembiol.2011.12.020_bib20","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1093\/clinchem\/39.9.1953","article-title":"Rare earth cryptates and homogeneous fluoroimmunoassays with human sera","volume":"39","author":"Mathis","year":"1993","journal-title":"Clin. Chem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib21","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0076-6879(06)12005-4","article-title":"Proteomics of polyglutamine aggregates","volume":"412","author":"Mitsui","year":"2006","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.chembiol.2011.12.020_bib22","doi-asserted-by":"crossref","first-page":"e8025","DOI":"10.1371\/journal.pone.0008025","article-title":"Formation of polyglutamine inclusions in a wide range of non-CNS tissues in the HdhQ150 knock-in mouse model of Huntington's disease","volume":"4","author":"Moffitt","year":"2009","journal-title":"PLoS ONE"},{"key":"10.1016\/j.chembiol.2011.12.020_bib23","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1602\/neurorx.1.2.255","article-title":"Huntington's disease genetics","volume":"1","author":"Myers","year":"2004","journal-title":"NeuroRx"},{"key":"10.1016\/j.chembiol.2011.12.020_bib24","doi-asserted-by":"crossref","first-page":"21807","DOI":"10.1074\/jbc.M109.084434","article-title":"Tracking mutant huntingtin aggregation kinetics in cells reveals three major populations that include an invariant oligomer pool","volume":"285","author":"Olshina","year":"2010","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib25","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1002\/(SICI)1097-4547(19961101)46:3<283::AID-JNR1>3.0.CO;2-G","article-title":"Amyloid precursor protein truncated at any of the gamma-secretase sites is not cleaved to beta-amyloid","volume":"46","author":"Paganetti","year":"1996","journal-title":"J.\u00a0Neurosci. Res."},{"key":"10.1016\/j.chembiol.2011.12.020_bib26","doi-asserted-by":"crossref","first-page":"10855","DOI":"10.1074\/jbc.M804813200","article-title":"Mutant huntingtin N-terminal fragments of specific size mediate aggregation and toxicity in neuronal cells","volume":"284","author":"Ratovitski","year":"2009","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib27","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1093\/hmg\/8.5.813","article-title":"Formation of polyglutamine inclusions in non-CNS tissue","volume":"8","author":"Sathasivam","year":"1999","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.chembiol.2011.12.020_bib28","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1093\/hmg\/ddp467","article-title":"Identical oligomeric and fibrillar structures captured from the brains of R6\/2 and knock-in mouse models of Huntington's disease","volume":"19","author":"Sathasivam","year":"2010","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.chembiol.2011.12.020_bib29","doi-asserted-by":"crossref","first-page":"4604","DOI":"10.1073\/pnas.96.8.4604","article-title":"Self-assembly of polyglutamine-containing huntingtin fragments into amyloid-like fibrils: implications for Huntington's disease pathology","volume":"96","author":"Scherzinger","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chembiol.2011.12.020_bib30","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1002\/cne.20680","article-title":"Chronology of behavioral symptoms and neuropathological sequela in R6\/2 Huntington's disease transgenic mice","volume":"490","author":"Stack","year":"2005","journal-title":"J.\u00a0Comp. Neurol."},{"key":"10.1016\/j.chembiol.2011.12.020_bib31","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1016\/0092-8674(93)90585-E","article-title":"A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes","volume":"72","year":"1993","journal-title":"Cell"},{"key":"10.1016\/j.chembiol.2011.12.020_bib32","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.nbd.2007.01.006","article-title":"Testicular degeneration in Huntington disease","volume":"26","author":"Van Raamsdonk","year":"2007","journal-title":"Neurobiol. Dis."},{"key":"10.1016\/j.chembiol.2011.12.020_bib33","doi-asserted-by":"crossref","first-page":"2738","DOI":"10.1093\/hmg\/ddn175","article-title":"Accumulation of N-terminal mutant huntingtin in mouse and monkey models implicated as a pathogenic mechanism in Huntington's disease","volume":"17","author":"Wang","year":"2008","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.chembiol.2011.12.020_bib34","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1515\/BC.2000.114","article-title":"Protein aggregation and pathogenesis of Huntington's disease: mechanisms and correlations","volume":"381","author":"Wanker","year":"2000","journal-title":"Biol. Chem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib35","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0076-6879(99)09026-6","article-title":"Membrane filter assay for detection of amyloid-like polyglutamine-containing protein aggregates","volume":"309","author":"Wanker","year":"1999","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.chembiol.2011.12.020_bib36","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1111\/j.1471-4159.2007.05032.x","article-title":"Sensitive biochemical aggregate detection reveals aggregation onset before symptom development in cellular and murine models of Huntington's disease","volume":"104","author":"Weiss","year":"2008","journal-title":"J.\u00a0Neurochem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib37","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ab.2009.08.001","article-title":"Single-step detection of mutant huntingtin in animal and human tissues: a bioassay for Huntington's disease","volume":"395","author":"Weiss","year":"2009","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib38","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.ab.2010.11.044","article-title":"Microtiter plate quantification of mutant and wild-type huntingtin normalized to cell count","volume":"410","author":"Weiss","year":"2011","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.chembiol.2011.12.020_bib39","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.brainresbull.2006.11.004","article-title":"The Hdh(Q150\/Q150) knock-in mouse model of HD and the R6\/2 exon 1 model develop comparable and widespread molecular phenotypes","volume":"72","author":"Woodman","year":"2007","journal-title":"Brain Res. Bull."}],"container-title":["Chemistry &amp; Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1074552112000166?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1074552112000166?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T07:17:48Z","timestamp":1761031068000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1074552112000166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["S1074552112000166"],"URL":"https:\/\/doi.org\/10.1016\/j.chembiol.2011.12.020","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.717951315.793456621","asserted-by":"object"}]},"ISSN":["1074-5521"],"issn-type":[{"value":"1074-5521","type":"print"}],"subject":[],"published":{"date-parts":[[2012,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"TR-FRET-Based Duplex Immunoassay Reveals an Inverse Correlation of Soluble and Aggregated Mutant huntingtin in Huntington's Disease","name":"articletitle","label":"Article Title"},{"value":"Chemistry & Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chembiol.2011.12.020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2012 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}