{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T18:07:53Z","timestamp":1770487673285,"version":"3.49.0"},"reference-count":39,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,5,19]],"date-time":"2017-05-19T00:00:00Z","timestamp":1495152000000},"content-version":"vor","delay-in-days":383,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01AI078962"],"award-info":[{"award-number":["R01AI078962"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01AI014102-37S1"],"award-info":[{"award-number":["R01AI014102-37S1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"AHA fellowship","award":["14POST18970046"],"award-info":[{"award-number":["14POST18970046"]}]},{"name":"Fundac\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["2011\/51973-3"],"award-info":[{"award-number":["2011\/51973-3"]}]},{"name":"Fundac\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["2012\/09403-8"],"award-info":[{"award-number":["2012\/09403-8"]}]},{"name":"Fundac\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["2013\/20074-9"],"award-info":[{"award-number":["2013\/20074-9"]}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["477143\/2011-3"],"award-info":[{"award-number":["477143\/2011-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Chemical Biology"],"published-print":{"date-parts":[[2016,5]]},"DOI":"10.1016\/j.chembiol.2016.03.015","type":"journal-article","created":{"date-parts":[[2016,4,30]],"date-time":"2016-04-30T17:32:44Z","timestamp":1462037564000},"page":"608-617","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"title":["Chemogenetic Characterization of Inositol Phosphate Metabolic Pathway Reveals Druggable Enzymes for Targeting Kinetoplastid Parasites"],"prefix":"10.1016","volume":"23","author":[{"given":"Igor","family":"Cestari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paige","family":"Haas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nilmar\u00a0Silvio","family":"Moretti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Schenkman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Stuart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.chembiol.2016.03.015_bib1","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1101\/gr.115089.110","article-title":"High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome","volume":"21","author":"Alsford","year":"2011","journal-title":"Genome Res."},{"key":"10.1016\/j.chembiol.2016.03.015_bib2","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkp851","article-title":"TriTrypDB: a functional genomic resource for the Trypanosomatidae","volume":"38","author":"Aslett","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.chembiol.2016.03.015_bib3","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.1056\/NEJMra1410150","article-title":"Chagas' disease","volume":"373","author":"Bern","year":"2015","journal-title":"N.\u00a0Engl. J. Med."},{"key":"10.1016\/j.chembiol.2016.03.015_bib4","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1093\/bioinformatics\/btn307","article-title":"ChemmineR: a compound mining framework for R","volume":"24","author":"Cao","year":"2008","journal-title":"Bioinformatics"},{"key":"10.1016\/j.chembiol.2016.03.015_bib5","doi-asserted-by":"crossref","first-page":"e9721","DOI":"10.1371\/journal.pone.0009721","article-title":"Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells","volume":"5","author":"Cestari","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.chembiol.2016.03.015_bib6","doi-asserted-by":"crossref","first-page":"14256","DOI":"10.1074\/jbc.M112.447441","article-title":"Inhibition of isoleucyl-tRNA synthetase as a potential treatment for human African trypanosomiasis","volume":"288","author":"Cestari","year":"2013","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chembiol.2016.03.015_bib7","doi-asserted-by":"crossref","first-page":"E2803","DOI":"10.1073\/pnas.1501206112","article-title":"Inositol phosphate pathway controls transcription of telomeric expression sites in trypanosomes","volume":"112","author":"Cestari","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chembiol.2016.03.015_bib8","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.devcel.2015.06.016","article-title":"Modulation of ciliary phosphoinositide content regulates trafficking and sonic hedgehog signaling output","volume":"34","author":"Chavez","year":"2015","journal-title":"Dev. Cell"},{"key":"10.1016\/j.chembiol.2016.03.015_bib9","doi-asserted-by":"crossref","first-page":"2480","DOI":"10.1128\/AAC.00281-10","article-title":"A nonazole CYP51\u00a0inhibitor cures Chagas' disease in a mouse model of acute infection","volume":"54","author":"Doyle","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.chembiol.2016.03.015_bib10","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1126\/science.1112181","article-title":"Comparative genomics of trypanosomatid parasitic protozoa","volume":"309","author":"El-Sayed","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.chembiol.2016.03.015_bib11","doi-asserted-by":"crossref","first-page":"35360","DOI":"10.1074\/jbc.M112.365031","article-title":"Structural studies and protein engineering of inositol phosphate multikinase","volume":"287","author":"Endo-Streeter","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chembiol.2016.03.015_bib12","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/nature09107","article-title":"Thousands of chemical starting points for antimalarial lead identification","volume":"465","author":"Gamo","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.chembiol.2016.03.015_bib13","doi-asserted-by":"crossref","first-page":"27600","DOI":"10.1074\/jbc.M602183200","article-title":"TbVps34, the trypanosome orthologue of Vps34, is required for Golgi complex segregation","volume":"281","author":"Hall","year":"2006","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chembiol.2016.03.015_bib14","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1111\/mmi.12155","article-title":"Inositol 1,4,5-trisphosphate receptor regulates replication, differentiation, infectivity and virulence of the parasitic protist Trypanosoma cruzi","volume":"87","author":"Hashimoto","year":"2013","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.chembiol.2016.03.015_bib15","doi-asserted-by":"crossref","first-page":"38109","DOI":"10.1074\/jbc.M606883200","article-title":"Crystal structure of inositol phosphate multikinase 2 and implications for substrate specificity","volume":"281","author":"Holmes","year":"2006","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chembiol.2016.03.015_bib16","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1073\/pnas.1216955110","article-title":"Acidocalcisomes of Trypanosoma brucei have an inositol 1,4,5-trisphosphate receptor that is required for growth and infectivity","volume":"110","author":"Huang","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chembiol.2016.03.015_bib17","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1128\/EC.00017-14","article-title":"Genetic validation of aminoacyl-tRNA synthetases as drug targets in Trypanosoma brucei","volume":"13","author":"Kalidas","year":"2014","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.chembiol.2016.03.015_bib18","doi-asserted-by":"crossref","first-page":"e1005058","DOI":"10.1371\/journal.ppat.1005058","article-title":"Utilizing chemical genomics to identify cytochrome b as a novel drug target for Chagas disease","volume":"11","author":"Khare","year":"2015","journal-title":"Plos Pathog."},{"key":"10.1016\/j.chembiol.2016.03.015_bib19","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1073\/pnas.1017831108","article-title":"Inositol polyphosphate multikinase is a physiologic PI3-kinase that activates Akt\/PKB","volume":"108","author":"Maag","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chembiol.2016.03.015_bib20","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1042\/BST0330983","article-title":"The myo-inositol-1-phosphate synthase gene is essential in Trypanosoma brucei","volume":"33","author":"Martin","year":"2005","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.chembiol.2016.03.015_bib21","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1365-2958.2006.05216.x","article-title":"The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream-form Trypanosoma brucei is dependent on the de novo synthesis of inositol","volume":"61","author":"Martin","year":"2006","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.chembiol.2016.03.015_bib22","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1042\/BJ20051825","article-title":"Phosphatidylinositol synthesis is essential in bloodstream form Trypanosoma brucei","volume":"396","author":"Martin","year":"2006","journal-title":"Biochem. J."},{"key":"10.1016\/j.chembiol.2016.03.015_bib23","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1038\/nature14412","article-title":"A molecular mechanism of artemisinin resistance in Plasmodium falciparum malaria","volume":"520","author":"Mbengue","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.chembiol.2016.03.015_bib24","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1006\/jmbi.1994.0137","article-title":"The crystal structure of cruzain: a therapeutic target for Chagas' disease","volume":"247","author":"McGrath","year":"1995","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.chembiol.2016.03.015_bib25","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.molcel.2013.05.020","article-title":"Class I HDACs share a common mechanism of regulation by inositol phosphates","volume":"51","author":"Millard","year":"2013","journal-title":"Mol. Cell"},{"key":"10.1016\/j.chembiol.2016.03.015_bib26","doi-asserted-by":"crossref","first-page":"4669","DOI":"10.1128\/AAC.04694-14","article-title":"Characterization of Trypanosoma cruzi sirtuins as possible drug targets for Chagas disease","volume":"59","author":"Moretti","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.chembiol.2016.03.015_bib27","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1177\/1087057114548832","article-title":"Identification of potent inhibitors of the Trypanosoma brucei methionyl-tRNA synthetase via high-throughput orthogonal screening","volume":"20","author":"Pedro-Rosa","year":"2015","journal-title":"J.\u00a0Biomol. Screen."},{"key":"10.1016\/j.chembiol.2016.03.015_bib28","doi-asserted-by":"crossref","first-page":"8771","DOI":"10.1038\/srep08771","article-title":"New compound sets identified from high throughput phenotypic screening against three kinetoplastid parasites: an open resource","volume":"5","author":"Pena","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.chembiol.2016.03.015_bib29","doi-asserted-by":"crossref","first-page":"e2977","DOI":"10.1371\/journal.pntd.0002977","article-title":"Synergy testing of FDA-approved drugs identifies potent drug combinations against Trypanosoma cruzi","volume":"8","author":"Planer","year":"2014","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.chembiol.2016.03.015_bib30","series-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2015"},{"key":"10.1016\/j.chembiol.2016.03.015_bib31","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/nchembio837","article-title":"Drug discovery and development for neglected parasitic diseases","volume":"2","author":"Renslo","year":"2006","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.chembiol.2016.03.015_bib32","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1128\/EC.00107-07","article-title":"Phosphatidylinositol 4-kinase III-beta is required for Golgi maintenance and cytokinesis in Trypanosoma brucei","volume":"6","author":"Rodgers","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.chembiol.2016.03.015_bib33","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.jmb.2008.10.071","article-title":"Structure and function of HIV-1 reverse transcriptase: molecular mechanisms of polymerization and inhibition","volume":"385","author":"Sarafianos","year":"2009","journal-title":"J.\u00a0Mol. Biol."},{"key":"10.1016\/j.chembiol.2016.03.015_bib34","doi-asserted-by":"crossref","first-page":"15660","DOI":"10.1073\/pnas.1514684112","article-title":"Inositol phosphate kinase 2 is required for imaginal disc development in Drosophila","volume":"112","author":"Seeds","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chembiol.2016.03.015_bib35","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1126\/science.1078062","article-title":"Regulation of chromatin remodeling by inositol polyphosphates","volume":"299","author":"Steger","year":"2003","journal-title":"Science"},{"key":"10.1016\/j.chembiol.2016.03.015_bib36","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1172\/JCI33945","article-title":"Kinetoplastids: related protozoan pathogens, different diseases","volume":"118","author":"Stuart","year":"2008","journal-title":"J.\u00a0Clin. Invest."},{"key":"10.1016\/j.chembiol.2016.03.015_bib37","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nature10728","article-title":"Structure of\u00a0HDAC3 bound to co-repressor and inositol tetraphosphate","volume":"481","author":"Watson","year":"2012","journal-title":"Nature"},{"key":"10.1016\/j.chembiol.2016.03.015_bib38","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.molcel.2013.08.031","article-title":"Human inositol polyphosphate multikinase regulates transcript-selective nuclear mRNA export to preserve genome integrity","volume":"51","author":"Wickramasinghe","year":"2013","journal-title":"Mol. Cell"},{"key":"10.1016\/j.chembiol.2016.03.015_bib39","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1242\/jcs.180141","article-title":"PI3K regulates endocytosis after insulin secretion via signaling crosstalk between Arf6 and Rab27a","volume":"129","author":"Yamaoka","year":"2015","journal-title":"J.\u00a0Cell Sci."}],"container-title":["Cell Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2451945616300939?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2451945616300939?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,19]],"date-time":"2020-09-19T08:48:05Z","timestamp":1600505285000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2451945616300939"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":39,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["S2451945616300939"],"URL":"https:\/\/doi.org\/10.1016\/j.chembiol.2016.03.015","relation":{},"ISSN":["2451-9456"],"issn-type":[{"value":"2451-9456","type":"print"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Chemogenetic Characterization of Inositol Phosphate Metabolic Pathway Reveals Druggable Enzymes for Targeting Kinetoplastid Parasites","name":"articletitle","label":"Article Title"},{"value":"Cell Chemical Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chembiol.2016.03.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}