{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:38:11Z","timestamp":1787236691269,"version":"build-2736575974"},"reference-count":41,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemosphere"],"published-print":{"date-parts":[[2007,4]]},"DOI":"10.1016\/j.chemosphere.2006.11.032","type":"journal-article","created":{"date-parts":[[2007,1,11]],"date-time":"2007-01-11T07:58:14Z","timestamp":1168502294000},"page":"1144-1154","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":57,"title":["Environmental monitoring by gene expression biomarkers in Barbus graellsii: Laboratory and field studies"],"prefix":"10.1016","volume":"67","author":[{"given":"Laia","family":"Quir\u00f3s","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Pi\u00f1a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montse","family":"Sol\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juli\u00e1n","family":"Blasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel \u00c1ngel","family":"L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Carmen","family":"Riva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dami\u00e0","family":"Barcel\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demetrio","family":"Rald\u00faa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chemosphere.2006.11.032_bib1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.envres.2005.07.002","article-title":"Analysis of vitellogenin gene induction as a valuable biomarker of estrogenic exposure in various Mediterranean fish species","volume":"101","author":"Barucca","year":"2006","journal-title":"Environ. Res."},{"issue":"Supplement A","key":"10.1016\/j.chemosphere.2006.11.032_bib2","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/j.1095-8649.2001.tb01386.x","article-title":"Anthropogenic changes in the freshwater fish fauna of Italy, with reference to the central region and Barbus graellsii, a newly established alien species of Iberian origin","volume":"59","author":"Bianco","year":"2001","journal-title":"J. Fish Biol."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib3","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/S0090-9556(25)05659-4","article-title":"Ethoxyresorufin \u2013 direct fluorimetric assay of a microsomal o-dealkylation which is preferentially inducible by 3-methylcholanthrene","volume":"2","author":"Burke","year":"1974","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib4","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.bbaexp.2003.11.009","article-title":"Common carp metallothionein gene: cDNA cloning, gene structure and expression studies","volume":"1676","author":"Chan","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib5","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.bbaexp.2005.09.006","article-title":"Tilapia metallothionein genes: PCR-cloning and gene expression studies","volume":"1731","author":"Cheung","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib6","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1111\/j.1095-8649.2002.tb01853.x","article-title":"Phylogenetic relationships of Euroasian and American cyprinids using cytochrome b sequences","volume":"61","author":"Cunha","year":"2002","journal-title":"J. Fish Biol."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib7","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0141-1136(02)00189-7","article-title":"Application of real time PCR determination to assess interanimal variability in CYP1A induction in the European flounder (Platicthys flesus)","volume":"54","author":"Dixon","year":"2002","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib8","series-title":"Peces Continentales Espa\u00f1oles. Coleccion T\u00e9cnica","author":"Doadrio","year":"1991"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib9","doi-asserted-by":"crossref","first-page":"321","DOI":"10.23818\/limn.20.29","article-title":"Peces y sedimentos: dos matrices para la vigilancia de la contaminaci\u00f3n por sustancias peligrosas en la cuenca del r\u00edo Ebro","volume":"20","author":"Dur\u00e1n","year":"2001","journal-title":"Limnetica"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib10","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1021\/es0301424","article-title":"Occurrence of polybrominated diphenylethers and hexabromocyclododecane in sediments and fish from Cinca River (Spain)","volume":"38","author":"Eljarrat","year":"2004","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib11","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0045-6535(98)00167-2","article-title":"Occurrence of organochlorine insecticides, PCBs and PCB congeners in waters and sediments of the Ebro river (Spain)","volume":"38","author":"Fernandez","year":"1999","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib12","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/0305-0491(85)90292-5","article-title":"Storage conditions of rainbow trout liver cytochrome P-450 and conjugating enzymes","volume":"80","author":"F\u00f6rlin","year":"1985","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib13","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1007\/s00216-003-2295-1","article-title":"Use of vitellogenin mRNA as a biomarker for endocrine disruption in feral and cultured fish","volume":"378","author":"Garc\u00eda-Reyero","year":"2004","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib14","doi-asserted-by":"crossref","first-page":"3972","DOI":"10.1021\/es0483490","article-title":"Comparative effects of dietary methylmercury on gene expression in liver, skeletal muscle, and brain of the zebrafish (Daniorerio)","volume":"39","author":"Gonzalez","year":"2005","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib15","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.marenvres.2004.03.059","article-title":"Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of metal exposure","volume":"58","author":"Hayes","year":"2004","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib16","first-page":"457","article-title":"Tissue-specific expression of two metallothionein genes in common carp during cadmium exposure and temperature shock","volume":"128","author":"Hermesz","year":"2001","journal-title":"Biochem. Physiol. Part C"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib17","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.envpol.2005.08.008","article-title":"Pilot survey of a broad range of priority pollutants in sediment and fish from the Ebro river basin (NE Spain)","volume":"140","author":"Lacorte","year":"2006","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib18","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.envpol.2005.05.003","article-title":"The combined use of chemical and biochemical markers to assess water quality along the Ebro River","volume":"139","author":"Lavado","year":"2006","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib19","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1098\/rspb.2001.1654","article-title":"Evolutionary history and speciation modes in the cyprinid genus Barbus","volume":"268","author":"Machordom","year":"2001","journal-title":"Proc. R. Soc. Lond. B"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib20","doi-asserted-by":"crossref","first-page":"2337","DOI":"10.1897\/02-495","article-title":"Expression and inducibility of aryl hydrocarbon receptor pathway genes in wild-caught killifish (Fundulus heteroclitus) with different contaminant-exposure histories","volume":"22","author":"Meyer","year":"2003","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib21","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0166-445X(98)00075-7","article-title":"Induction of cytochrome P4501A (CYP1A) in Trematomus bernacchii as an indicator of environmental pollution in Antarctica: assessment by quantitative RT-PCR","volume":"44","author":"Miller","year":"1999","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib22","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0076-6879(91)05100-A","article-title":"Electrochemical detection of metallothionein","volume":"205","author":"Olafson","year":"1991","journal-title":"Method. Enzymol."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib23","doi-asserted-by":"crossref","first-page":"2146","DOI":"10.1002\/etc.5620211018","article-title":"Endocrine disruptors in sewage treatment plants, receiving river water, and sediments: integration of chemical analysis and biological effects on feral carp","volume":"21","author":"Petrovic","year":"2002","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib24","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib25","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1007\/s001289900232","article-title":"Mercury concentration in three different species of freshwater fish from Gallego and Cinca rivers","volume":"57","author":"Rald\u00faa","year":"1996","journal-title":"Spain. Bull. Environ. Contam. Toxicol."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib26","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1016\/S0045-6535(97)00303-2","article-title":"The influence of place of capture, sex, and season on the organochlorine pesticide content in barbel (Barbus graellsi) from northeastern Spain","volume":"35","author":"Rald\u00faa","year":"1997","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib27","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/j.chemosphere.2006.07.053","article-title":"Mercury levels and liver pathology in feral fish living in the vicinity of a mercury cell chlor-alkali factory","volume":"66","author":"Rald\u00faa","year":"2006","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib28","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.aquatox.2003.10.004","article-title":"Development and application of a real-time quantitative PCR assay for determining CYP1A transcripts in three genera of salmonids","volume":"66","author":"Rees","year":"2004","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib29","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1006\/eesa.1995.1075","article-title":"Expression of hepatic metallothionein messenger RNA in feral and caged fish species correlates with muscle mercury levels","volume":"31","author":"Schlenk","year":"1995","journal-title":"Ecotox. Environ. Saf."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib30","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1080\/135475097231698","article-title":"Characterization of hepatic metallothionein expression in channel catfish (Ictalurus punctatus) by reverse-transcriptase polymerase chain reaction","volume":"2","author":"Schlenk","year":"1997","journal-title":"Biomarkers"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib31","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1023\/B:EMAS.0000003594.64248.0a","article-title":"Concentrations of arsenic, cadmium, copper, lead, selenium, and zinc in fish from the Mississippi River basin, 1995","volume":"90","author":"Schmitt","year":"2004","journal-title":"Environ. Monit. Assess."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib32","first-page":"145","article-title":"Feminization of wild carp, Cyprinus carpio, in a polluted environment: plasma steroid hormones, gonadal morphology and xenobiotic metabolizing system","volume":"136","author":"Sol\u00e9","year":"2003","journal-title":"Comp. Biochem. Physiol. Part C"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib33","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/0076-6879(90)82008-P","article-title":"Quantitation of protein","volume":"182","author":"Stoscheck","year":"1990","journal-title":"Method Enzymol."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1476-069X-1-1","article-title":"Serum organochlorines and urinary porphyrin pattern in a population highly exposed to hexachlorobenzene","volume":"1","author":"Sunyer","year":"2002","journal-title":"Environ. Health"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib35","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1007\/s10646-005-0020-x","article-title":"Metallothionein mRNA expression and cadmium tolerance in metal-stressed and reference populations of the springtail Orchesella cincta","volume":"14","author":"Timmermans","year":"2005","journal-title":"Ecotoxicology"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib36","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.chemosphere.2004.10.063","article-title":"Transcript and protein environmental biomarkers in fish \u2013 a review","volume":"59","author":"Tom","year":"2005","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2006.11.032_bib37","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0025-326X(98)90188-6","article-title":"Monitoring of hepatic metallothionein mRNA levels in the fish Lithognathus mormyrus \u2013 evaluation of transition metal pollution in a mediterranean coast","volume":"38","author":"Tom","year":"1999","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib38","doi-asserted-by":"crossref","first-page":"2088","DOI":"10.1897\/02-275","article-title":"Quantitative evaluation of hepatic cytochrome P4501A transcript, protein, and catalytic activity in the striped sea bream (Lithognathus mormyrus)","volume":"22","author":"Tom","year":"2003","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib39","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.marpolbul.2003.10.013","article-title":"Quantifying fish metallothionein transcript by real time PCR for its utilization as an environmental biomarker","volume":"48","author":"Tom","year":"2004","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib40","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S1382-6689(02)00126-6","article-title":"Fish bioaccumulation and biomarkers in environmental risk assessment: a review","volume":"13","author":"van der Oost","year":"2003","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"10.1016\/j.chemosphere.2006.11.032_bib41","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1098\/rspb.1998.0443","article-title":"Phylogenetic relationships of Iberian cyprinids: systematic and biogeographical implications","volume":"265","author":"Zardoya","year":"1998","journal-title":"Proc. R. Soc. Lond. B"}],"container-title":["Chemosphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653506015566?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653506015566?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T09:00:10Z","timestamp":1758618010000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0045653506015566"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":41,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["S0045653506015566"],"URL":"https:\/\/doi.org\/10.1016\/j.chemosphere.2006.11.032","relation":{},"ISSN":["0045-6535"],"issn-type":[{"value":"0045-6535","type":"print"}],"subject":[],"published":{"date-parts":[[2007,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Environmental monitoring by gene expression biomarkers in Barbus graellsii: Laboratory and field studies","name":"articletitle","label":"Article Title"},{"value":"Chemosphere","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chemosphere.2006.11.032","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2006 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}