{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T17:15:18Z","timestamp":1768929318921,"version":"3.49.0"},"reference-count":61,"publisher":"Elsevier BV","issue":"9","content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemosphere"],"published-print":{"date-parts":[[2013,8]]},"DOI":"10.1016\/j.chemosphere.2013.01.102","type":"journal-article","created":{"date-parts":[[2013,3,6]],"date-time":"2013-03-06T00:49:34Z","timestamp":1362530974000},"page":"1067-1076","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":48,"title":["Test concentration setting for fish in vivo endocrine screening assays"],"prefix":"10.1016","volume":"92","author":[{"given":"James R.","family":"Wheeler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grace H.","family":"Panter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lennart","family":"Weltje","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen L.","family":"Thorpe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.chemosphere.2013.01.102_b0005","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1897\/1551-5028(2001)020<1276:DAEOAS>2.0.CO;2","article-title":"Description and evaluation of a short-term reproduction test with the fathead minnow (Pimephales promelas)","volume":"20","author":"Ankley","year":"2001","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0010","first-page":"1791","article-title":"Survey of estrogenic activity in United Kingdom estuarine and coastal waters and its effect on gonadal development of the flounder Platichthys flesus","volume":"18","author":"Arme","year":"1997","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0015","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1023\/B:ECTX.0000037188.09072.de","article-title":"Experimental designs to assess endocrine disrupting effects in invertebrates: a review","volume":"13","author":"Barata","year":"2004","journal-title":"Ecotoxicology"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0020","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.yrtph.2010.09.003","article-title":"Science based guidance for the assessment of endocrine disrupting properties of chemicals","volume":"59","author":"Bars","year":"2011","journal-title":"Regul. Toxicol. Pharm."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0025","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/BF00203763","article-title":"Responses of invertebrates and fish to alkyl sulfate and alkyl ethoxylate sulfate anionic surfactants during chronic exposure","volume":"55","author":"Belanger","year":"1995","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0030","unstructured":"Benoit, D., 1981. User\u2019s guide for conducting life-cycle chronic toxicity testing with fathead minnows (Pimephales promelas). United States Environmental Protection Agency, Washington, DC. EPA-600\/8-81-001."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0035","unstructured":"Braunbeck, T., Schaefers, C., Teigler, M., Knacker, T., 2009. Studies to Support the Validation of a Definitive Fish Tests, Full Life-Cycle\/Two-Generation, pp. 1\u201351."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0040","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/0166-445X(85)90026-8","article-title":"Acute toxicity of organic chemical mixtures to the fathead minnow","volume":"6","author":"Broderius","year":"1985","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0045","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1002\/etc.5620140920","article-title":"Use of joint toxic response to define the primary mode of toxic action for diverse industrial organic chemicals","volume":"14","author":"Broderius","year":"1995","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0050","series-title":"Effects of Ammonium Perchlorate, 4(Tert-Octyl)Phenol and Their Mixture on Zebrafish (Danio rerio)","author":"Cruz-Li","year":"2004"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0055","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0044-8486(02)00057-1","article-title":"Sex determination and sex differentiation in fish: an overview of genetic, physiological and environmental influences","volume":"208","author":"Devlin","year":"2002","journal-title":"Aquaculture"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0060","unstructured":"ECETOC, 2007. Intelligent Testing Strategies in Ecotoxicology: Mode of Action Approach for Specifically Acting Chemicals (Technical Report No. 102). European Centre for Ecotoxicology and Toxicology of Chemicals, Brussels."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0065","unstructured":"ECETOC, 2009. Guidance on Identifying Endocrine Disrupting Effects European Centre for Ecotoxicology and Toxicology of Chemicals, Brussels."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0070","unstructured":"EMEA, 2006. Guideline on the environmental risk assessment of medicinal products for human use. Committee for Medicinal Products for Human Use (CHMP) Document Reference EMEA\/CHMP\/SWP\/4447\/00 Issued London. London, UK (01.06.06)."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0075","doi-asserted-by":"crossref","first-page":"18","DOI":"10.2307\/3433048","article-title":"Principles for the selection of doses in chronic rodent bioassays. ILSI Risk Science Working Group on Dose Selection","volume":"105","author":"Foran","year":"1997","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0080","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1093\/icb\/45.1.19","article-title":"Is nitrate an ecologically relevant endocrine disruptor in vertebrates?","volume":"45","author":"Guillette","year":"2005","journal-title":"Integr. Comp. Biol."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0085","first-page":"57","article-title":"Detection of endocrine disrupters: evaluation of a Fish Sexual Development Test (FSDT)","volume":"144","author":"Holbech","year":"2006","journal-title":"Comp. Biochem. Physiol. C"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0090","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/0143-1471(84)90081-3","article-title":"The acute toxicity of selected substituted phenols, benzenes and benzoic acid esters to fathead minnows Pimephales promelas","volume":"35","author":"Holcombe","year":"1984","journal-title":"Environ. Pollut. Ser. A"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0095","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.yrtph.2009.05.013","article-title":"Variability of in vivo fish acute toxicity data","volume":"54","author":"Hrovat","year":"2009","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0100","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1007\/BF01688651","article-title":"Treatment of fish with hormones: solubilization and direct administration of steroids into aquaria water using acetone as a carrier solvent","volume":"47","author":"Hunsinger","year":"1991","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0105","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1289\/ehp.8062","article-title":"Screening and testing for endocrine disruption in fish \u2013 biomarkers as signposts not traffic lights in risk assessment","volume":"114","author":"Hutchinson","year":"2006","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0110","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.aquatox.2008.11.009","article-title":"Benefits of the maximum tolerated dose (MTD) and maximum tolerated concentration (MTC) concept in aquatic toxicology","volume":"91","author":"Hutchinson","year":"2009","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0115","first-page":"165","article-title":"Effects of the fungicide prochloraz on the sexual development of zebrafish (Danio rerio)","volume":"145","author":"Kinnberg","year":"2007","journal-title":"Comp. Biochem. Physiol. C"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0120","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1002\/ieam.92","article-title":"Environmental effect assessment for sexual endocrine-disrupting chemicals: fish testing strategy","volume":"6","author":"Knacker","year":"2010","journal-title":"IEAM"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0125","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.cbpa.2007.04.023","article-title":"Long term hypoxia suppresses reproductive capacity in the estuarine fish, Fundulus grandis","volume":"148","author":"Landry","year":"2007","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0130","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S0166-445X(99)00059-4","article-title":"Effects of the mammalian antiandrogen vinclozolin on development and reproduction of the fathead minnow (Pimephales promelas)","volume":"48","author":"Makynen","year":"2000","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0135","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/etc.5620060407","article-title":"Use of respiratory-cardiovascular responses of rainbow trout (Salmo gairdneri) in identifying acute toxicity syndromes in fish: Part 1 \u2013 Pentachlorophenol, 2,4-dinitrophenol, tricaine methanesulfonate and 1-octanol","volume":"6","author":"McKim","year":"1987","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0140","unstructured":"OECD, 1984. OECD 204 Fish, Prolonged Toxicity Test: 14-day Study. Organisation for Economic Cooperation and Development, Paris, pp. 1\u20139."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0145","unstructured":"OECD, 1992a. OECD 203 Fish Acute Toxicity Test. Organisation for Economic Cooperation and Development, Paris, pp. 1\u20139."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0150","unstructured":"OECD, 1992b. OECD 210 Fish, Early-life Stage Toxicity Test. Organisation for Economic Cooperation and Development, Paris, pp. 1\u201318."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0155","unstructured":"OECD, 1995. SIDS Initial Assessment Report: PHENOL, 4-(1,1,3,3-TETRAMETHYLBUTYL), pp. 1\u201354."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0160","unstructured":"OECD, 2000. OECD 215 Fish, Juvenile Growth Test. Organisation for Economic Cooperation and Development, Paris, pp. 1\u201317."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0165","unstructured":"OECD, 2006a. Final Draft Report: Phase 1B of the Validation of the 21-day Fish Assay for the Detection of Endocrine Active Substances, pp. 1\u2013129."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0170","unstructured":"OECD, 2006b. Final Draft Report for Phase 1A Validation of the Fish Screening Assay. Organisation for Economic Cooperation and Development, Paris."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0175","unstructured":"OECD, 2008. Detailed review paper on fish life-cycle tests. Organisation for Economic Cooperation and Development, Series on Testing and Assessment No. 95, Paris, p. 154."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0180","unstructured":"OECD, 2009a. OECD 229: Fish Short Term Reproduction Assay. Organisation for Economic Co-operation and Development, Paris, pp. 1\u201340."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0185","unstructured":"OECD, 2009b. OECD 230: 21-day Fish Assay: A short-term screening for oestrogenic and androgenic activity, and aromatase inhibition. Organisation for Economic Co-operation and Development, Paris, pp. 1\u201338."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0190","unstructured":"OECD, 2010. Guidance Document 123: Guidance Document on the Diagnosis of Endocrine-Related Histopathology in Fish Gonads. Organisation for Economic Cooperation and Development, Paris, pp. 1\u2013114."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0195","unstructured":"OECD, 2011. OECD 234: Fish Sexual Development Test. Organisation for Economic Co-operation and Development, Paris, pp. 1\u201344."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0200","unstructured":"OECD, 2012. Draft Guidance Document on Standardised Test Guidelines for Evaluating Chemicals for Endocrine Disruption. Organisation for Economic Cooperation and Development, Paris (April, 2012)."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0205","unstructured":"OPPTS, 2009. Endocrine Disruptor Screening Program Test Guidelines OPPTS 890.1350: Fish Short-Term Reproduction Assay. United States Environmental Protection Agency, pp. 1\u201323."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0210","unstructured":"Owens, J., 2007a. Report of Three 21-day Fish Endocrine Screening Assays To Complete CEFIC\u2019s Contribution to Phase-2 of the OECD Validation Program: Studies with Potassium Permanganate, n-Octanol, and 2-Methoxyethanol in the Fathead Minnow (Pimephales promelas), pp. 1\u201357."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0215","unstructured":"Owens, J., 2007b. Phase 3 Report of the Validation of the OECD Fish Screening Assay. CEFIC LRI."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0220","unstructured":"Panter, G., Tyler, C., Maddix, S., Campbell, P., Hutchinson, T., L\u00e4nge, R., Lye, C., Sumpter, J., 1999. Applications of an enzyme-linked immunosorbent assay (ELISA) to quantify vitellogenin concentrations in fathead minnows (Pimephales promelas) exposed to endocrine disrupting chemicals. CEFIC-EMSG AQ001."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0225","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.aquatox.2006.01.004","article-title":"Development of chronic tests for endocrine active chemicals: Part 1. An extended fish early-life stage test for oestrogenic active chemicals in the fathead minnow (Pimephales promelas)","volume":"77","author":"Panter","year":"2006","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0230","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.1897\/07-069R.1","article-title":"Determinants of variability in acute to chronic toxicity ratios for aquatic invertebrates and fish","volume":"26","author":"Raimondo","year":"2007","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0235","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s12302-008-0014-4","article-title":"Zur Entwicklung einer Pr\u00fcfstrategie auf sexual-endokrine Wirksamkeit einer chemischen Substanz bei Fischen","volume":"20","author":"Schaefers","year":"2008","journal-title":"Umweltwiss Schadst Forsch (UWSF)"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0240","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1002\/etc.5620220712","article-title":"Effects of 4-nonylphenol and 4-tert-octylphenol on sex differentiation and vitellogenin induction in medaka (Oryzias latipes)","volume":"22","author":"Seki","year":"2003","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0245","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1002\/etc.5620220710","article-title":"Fish full life-cycle testing for a weak estrogen 4-tert-pentylphenol on medaka (Oryzias latipes)","volume":"22","author":"Seki","year":"2003","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0250","series-title":"Physiological Ecology of Animals: An Evolutionary Approach","author":"Sibly","year":"1986"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0255","doi-asserted-by":"crossref","first-page":"2693","DOI":"10.1098\/rspb.2007.0921","article-title":"Widespread endocrine disruption and reproductive impairment in an estuarine fish population exposed to seasonal hypoxia","volume":"274","author":"Thomas","year":"2007","journal-title":"Proc. R Soc. B"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0260","series-title":"The Pesticide Manual","author":"Tomlin","year":"2003"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0265","unstructured":"US-EPA, 2002. A short-term test method for assessing the reproductive toxicity of endocrine-disrupting chemicals using the fathead minnow (Pimephales promelas). U.S. Environmental Protection Agency, Office of Research and Development National Health and Environmental Effects Research Laboratory, Mid-Continent Ecology Division, Duluth, MN 55804, Duluth, pp. 1\u2013154."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0270","unstructured":"US-EPA, 2006. Fathead Minnow (Pimephales promelas) Fish Screening Assay OECD Phase 1B Follow-up. Battelle, pp. 1\u2013656."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0275","unstructured":"US-EPA, 2007. Validation of the Fish Short-Term Reproduction Assay: Integrated Summary Report. U.S. Environmental Protection Agency Endocrine Disruptor Screening Program, Washington, D.C., pp. 1\u2013102."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0280","unstructured":"US-EPA, 2010. The ECOTOX (ECOTOXicology) Database Provides Single Chemical Toxicity Information for Aquatic and Terrestrial Life."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0285","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.cbpc.2008.03.014","article-title":"Hypoxia inhibits fish spawning via LH-dependent final oocyte maturation","volume":"148","author":"Wang","year":"2008","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0290","unstructured":"Wenzel, A., Schaefers, C., Vollmer, G., Michna, H., Diel, P., 2001. Research efforts towards the development and validation of a test method for the identification of endocrine disrupting chemicals. Fraunhofer-Institut f\u00fcr Umweltchemie und \u00d6kotoxikologie, pp. 1\u201382."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0295","doi-asserted-by":"crossref","first-page":"163","DOI":"10.3354\/dao026163","article-title":"Gonadal impairment in roach Rutilus rutilus from Finnish coastal areas of the northern Baltic sea","volume":"26","author":"Wiklund","year":"1996","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.chemosphere.2013.01.102_b0300","first-page":"232","article-title":"Ligula intestinalis (Cestoda) infections of roach (Rutilus rutilus) (Cyprinidae): immunocytochemical investigations into the salmon- and chicken-II type gonadotrophin-releasing hormone (GnRH) systems in host brains","volume":"43","author":"Williams","year":"1998","journal-title":"Acta Parasitologica"},{"key":"10.1016\/j.chemosphere.2013.01.102_b0305","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1021\/es0258327","article-title":"Aquatic hypoxia is an endocrine disruptor and impairs fish reproduction","volume":"37","author":"Wu","year":"2003","journal-title":"Environ. Sci. Technol."}],"container-title":["Chemosphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653513002579?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653513002579?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T07:55:44Z","timestamp":1539849344000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0045653513002579"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":61,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["S0045653513002579"],"URL":"https:\/\/doi.org\/10.1016\/j.chemosphere.2013.01.102","relation":{},"ISSN":["0045-6535"],"issn-type":[{"value":"0045-6535","type":"print"}],"subject":[],"published":{"date-parts":[[2013,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Test concentration setting for fish in vivo endocrine screening assays","name":"articletitle","label":"Article Title"},{"value":"Chemosphere","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chemosphere.2013.01.102","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}