{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T04:11:06Z","timestamp":1772683866546,"version":"3.50.1"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100014809","name":"Technology Agency of the Czech Republic","doi-asserted-by":"publisher","award":["LM2015073"],"award-info":[{"award-number":["LM2015073"]}],"id":[{"id":"10.13039\/100014809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","award":["CZ.02.1.01\/0.0\/0.0\/16_013\/0001821"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_013\/0001821"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemosphere"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1016\/j.chemosphere.2019.124460","type":"journal-article","created":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T17:34:59Z","timestamp":1564076099000},"page":"124460","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Engineered in situ biogeochemical transformation as a secondary treatment following ISCO \u2013 A field test"],"prefix":"10.1016","volume":"237","author":[{"given":"Jan","family":"N\u011bme\u010dek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magda","family":"Nechanick\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4854-3720","authenticated-orcid":false,"given":"Roman","family":"\u0160p\u00e1nek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franti\u0161ek","family":"Eichler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josef","family":"Zeman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miroslav","family":"\u010cern\u00edk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.chemosphere.2019.124460_bib1","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1021\/es990884q","article-title":"Pathway and kinetics of chlorinated ethylene and chlorinated acetylene reaction with Fe(0) particles","volume":"34","author":"Arnold","year":"2000","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.chemosphere.2019.124460_bib2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0009-2541(99)00198-9","article-title":"Reaction pathways in the Fe-S system below 100\u00b0C","volume":"167","author":"Benning","year":"2000","journal-title":"Chem. Geol."},{"key":"10.1016\/j.chemosphere.2019.124460_bib3","series-title":"Proceeding of the 13th International UFZ-Deltarez Conference on Sustainable Use and Management of Soil, Sediment and Water Resources","first-page":"9","article-title":"Meeting the challenges for bioremediation of chlorinated solvents at operational sites: a comparison of case studies","author":"Bewley","year":"2015"},{"key":"10.1016\/j.chemosphere.2019.124460_bib4","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0135868","article-title":"Microbial communities can Be described by metabolic structure: a general framework and application to a seasonally variable, depth-stratified microbial community from the coastal west antarctic peninsula","volume":"10","author":"Bowman","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.chemosphere.2019.124460_bib5","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: high-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"10.1016\/j.chemosphere.2019.124460_bib6","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1002\/etc.5620160404","article-title":"Trichloroethylene and tetrachloroethylene reduction in a metallic iron-water-vapor batch system","volume":"16","author":"Campbell","year":"1997","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2019.124460_bib7","article-title":"Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions","volume":"gkq873","author":"Claesson","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.chemosphere.2019.124460_bib8","series-title":"Water Quality. Determination of Ammonium. Part 1: Manual Spectrometric Method","author":"\u010cSN EN ISO 7150-1","year":"1994"},{"key":"10.1016\/j.chemosphere.2019.124460_bib9","series-title":"Water Quality \u2013 Determination of Dissolved Anions by Liquid Chromatography of Ions \u2013 Part 1: Determination of Bromide, Chloride, Fluoride, Nitrate, Nitrite, Phosphate and Sulfate","author":"\u010cSN EN ISO 10304-1","year":"2007"},{"key":"10.1016\/j.chemosphere.2019.124460_bib10","series-title":"Water Quality \u2013 Determination of Selected Elements by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES)","author":"\u010cSN EN ISO 11885","year":"2007"},{"key":"10.1016\/j.chemosphere.2019.124460_bib11","series-title":"Chemical and Physical Analysis of Surface Water. Determination of Sulphide and Hydrogen Sulphide","year":"1980"},{"key":"10.1016\/j.chemosphere.2019.124460_bib12","series-title":"Water Quality \u2013 Determination of Alkalinity \u2013 Part 1: Determination of Total and Composite Alkalinity","year":"1994"},{"key":"10.1016\/j.chemosphere.2019.124460_bib13","series-title":"Water Analysis - Guidelines for the Determination of Total Organic Carbon (TOC) and Dissolved Organic Carbon","year":"1998"},{"key":"10.1016\/j.chemosphere.2019.124460_bib14","series-title":"Water Quality. Sampling. Part 11: Guidance on Sampling of Groundwaters","year":"1993"},{"key":"10.1016\/j.chemosphere.2019.124460_bib15","doi-asserted-by":"crossref","first-page":"13262","DOI":"10.1007\/s11356-017-8867-y","article-title":"Microbial degradation of chloroethenes: a review","volume":"24","author":"Dolinov\u00e1","year":"2017","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.chemosphere.2019.124460_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2180-8-125","article-title":"Evaluation of the bacterial diversity in the faeces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP)","volume":"8","author":"Dowd","year":"2008","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.chemosphere.2019.124460_bib17","series-title":"Scale-up and Characterization of an Enrichment Culture for Bioaugumentation of the P-Area Chlorinated Ethene Plume at the Savannah River Site","author":"Eaddy","year":"2008"},{"key":"10.1016\/j.chemosphere.2019.124460_bib18","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/j.1574-6968.1987.tb02154.x","article-title":"Anaerobic dechlorination of tetrachloromethane and1,2-dichloroethanetodegradableproductsbypure cultures of Desulfobacterium sp. and Methanobacterium sp","volume":"43","author":"Egli","year":"1987","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.chemosphere.2019.124460_bib19","series-title":"Geomicrobiology","first-page":"606","author":"Ehrlich","year":"2009"},{"key":"10.1016\/j.chemosphere.2019.124460_bib20","series-title":"Identification and Characterization Methods for Reactive Minerals Responsible for Natural Attenuation of Chlorinated Organic Compounds in Ground Water","year":"2009"},{"issue":"11","key":"10.1016\/j.chemosphere.2019.124460_bib21","doi-asserted-by":"crossref","first-page":"2671","DOI":"10.1128\/aem.53.11.2671-2674.1987","article-title":"Anaerobic bacteria that dechlorinate perchloroethene","volume":"53","author":"Fathepure","year":"1987","journal-title":"Appl. Environ. Microbiol."},{"issue":"2","key":"10.1016\/j.chemosphere.2019.124460_bib22","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1128\/AEM.70.2.1234-1237.2004","article-title":"Electron transfer by Desulfobulbus propionicus to Fe (III) and graphite electrodes","volume":"70","author":"Holmes","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.chemosphere.2019.124460_bib23","doi-asserted-by":"crossref","first-page":"5","DOI":"10.2307\/3545743","article-title":"Statistics and partitioning of species diversity, and similarity among multiple communities","volume":"76","author":"Lande","year":"1996","journal-title":"Oikos"},{"issue":"1","key":"10.1016\/j.chemosphere.2019.124460_bib24","first-page":"1","article-title":"FactoMineR: an R package for multivariate analysis","volume":"25","author":"Le","year":"2008","journal-title":"J.\u00a0Stat. Softw."},{"key":"10.1016\/j.chemosphere.2019.124460_bib25","article-title":"Analyses of the microbial diversity across the human microbiome","volume":"7","author":"Li","year":"2012","journal-title":"PLoS One"},{"issue":"4","key":"10.1016\/j.chemosphere.2019.124460_bib26","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1016\/j.gca.2010.11.009","article-title":"Reduction of structural Fe(III) in nontronite by methanogen Methanosarcina barkeri","volume":"75","author":"Liu","year":"2011","journal-title":"Geochem. Cosmochim. Acta"},{"issue":"8","key":"10.1016\/j.chemosphere.2019.124460_bib27","doi-asserted-by":"crossref","first-page":"2402","DOI":"10.1128\/jb.178.8.2402-2408.1996","article-title":"Phylogenetic analysis of dissimilatory Fe (III)-reducing bacteria","volume":"178","author":"Lonergan","year":"1996","journal-title":"J.\u00a0Bacteriol."},{"issue":"1","key":"10.1016\/j.chemosphere.2019.124460_bib28","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1146\/annurev.mi.47.100193.001403","article-title":"Dissimilatory metal reduction","volume":"47","author":"Lovley","year":"1993","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.chemosphere.2019.124460_bib29","series-title":"Efficient Metabolic Exchange and Electron Transfer within a Syntrophic TCE Degrading Co-culture of Dehalococcoides Mccartyi 195 and Syntrophomonas Wolfei. Applied and Environmental Microbiology","author":"Mao","year":"2015"},{"key":"10.1016\/j.chemosphere.2019.124460_bib30","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1186\/1471-2105-11-538","article-title":"pplacer: linear time maximum-likelihood and Bayesian phylogenetic placement of sequences onto a fixed reference tree","volume":"11","author":"Matsen","year":"2010","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.chemosphere.2019.124460_bib31","series-title":"Biogeochemical Transformation Handbook","year":"2015"},{"key":"10.1016\/j.chemosphere.2019.124460_bib32","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1016\/j.chemosphere.2017.06.100","article-title":"Stratification of chlorinated ethenes natural attenuation in an alluvial aquifer assessed by hydrochemical and biomolecular tools","volume":"184","author":"N\u011bme\u010dek","year":"2017","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2019.124460_bib33","article-title":"Vegan: community ecology package","author":"Oksanen","year":"2012","journal-title":"R\u00a0package.\u2019ver."},{"key":"10.1016\/j.chemosphere.2019.124460_bib34","series-title":"Dense Chlorinated Solvents and Other DNAPLs in Groundwater: History, Behaviour, and Remediation","first-page":"525","author":"Pankow","year":"1996"},{"key":"10.1016\/j.chemosphere.2019.124460_bib35","doi-asserted-by":"crossref","first-page":"5779","DOI":"10.1016\/j.gca.2006.02.029","article-title":"The solubility of FeS","volume":"70","author":"Rickard","year":"2006","journal-title":"Geochem. Cosmochim. Acta"},{"key":"10.1016\/j.chemosphere.2019.124460_bib36","series-title":"Development of Optimal pH for Degradation of Chlorinated Solvents by KB-1TM Anaerobic Bacterial Culture","author":"Rowlands","year":"2004"},{"key":"10.1016\/j.chemosphere.2019.124460_bib37","series-title":"Chlorinated Solvent Source Zone Remediation","first-page":"253","article-title":"In situ chemical oxidation","author":"Siegrist","year":"2014"},{"key":"10.1016\/j.chemosphere.2019.124460_bib38","series-title":"In Situ Bioremediation of Chlorinated Ethene Source Zones. Chlorinated Solvent Source Zone Remediation","first-page":"395","author":"Stroo","year":"2014"},{"key":"10.1016\/j.chemosphere.2019.124460_bib39","series-title":"In Situ Biogeochemical Treatment Demonstration: Lessons Learned from ESTCP Project ER-201124","first-page":"80","author":"Stroo","year":"2015"},{"issue":"3","key":"10.1016\/j.chemosphere.2019.124460_bib42","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/gwmr.12068","article-title":"Factors controlling in situ biogeochemical transformation of trichloroethene: field survey","volume":"34","author":"Whiting","year":"2014","journal-title":"Groundwater Monitoring & Remediation"},{"key":"10.1016\/j.chemosphere.2019.124460_bib43","doi-asserted-by":"crossref","first-page":"5634","DOI":"10.1021\/es0487776","article-title":"Carbon isotope fractionation of carbon tetrachloride at iron (hydr)oxide and iron sulfide minerals","volume":"39","author":"Zwank","year":"2005","journal-title":"Environ. Sci. Technol."}],"container-title":["Chemosphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653519316844?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653519316844?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T10:32:51Z","timestamp":1758623571000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0045653519316844"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12]]},"references-count":41,"alternative-id":["S0045653519316844"],"URL":"https:\/\/doi.org\/10.1016\/j.chemosphere.2019.124460","relation":{},"ISSN":["0045-6535"],"issn-type":[{"value":"0045-6535","type":"print"}],"subject":[],"published":{"date-parts":[[2019,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Engineered in situ biogeochemical transformation as a secondary treatment following ISCO \u2013 A field test","name":"articletitle","label":"Article Title"},{"value":"Chemosphere","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chemosphere.2019.124460","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"124460"}}