{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T14:21:19Z","timestamp":1776694879890,"version":"3.51.2"},"reference-count":102,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BIA-BMA\/30922\/2017"],"award-info":[{"award-number":["PTDC\/BIA-BMA\/30922\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/\/00350\/2020"],"award-info":[{"award-number":["UID\/\/00350\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemosphere"],"published-print":{"date-parts":[[2020,12]]},"DOI":"10.1016\/j.chemosphere.2020.127678","type":"journal-article","created":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T16:33:05Z","timestamp":1594830785000},"page":"127678","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"special_numbering":"C","title":["Cytotoxic responses of the anticancer drug cyclophosphamide in the mussel Mytilus galloprovincialis and comparative sensitivity with human cells lines"],"prefix":"10.1016","volume":"261","author":[{"given":"Elna","family":"Fernandes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tain\u00e1 Garcia","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e2nia","family":"Carri\u00e7o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00e9lia","family":"Mestre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1lvaro","family":"Tavares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Jo\u00e3o","family":"Bebianno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.chemosphere.2020.127678_bib1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.envres.2015.10.028","article-title":"Is the step-wise tiered approach for ERA of pharmaceuticals useful for the assessment of cancer therapeutic drugs present in marine environment?","volume":"144","author":"Aguirre-Mart\u00ednez","year":"2016","journal-title":"Environ. Res."},{"key":"10.1016\/j.chemosphere.2020.127678_bib2","first-page":"661","article-title":"The effect of cyclophosphamide on the immune system: implications for clinical cancer therapy. Cancer Chemother","volume":"78","author":"Ahlmann","year":"2016","journal-title":"Pharmacology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib3","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/0378-4274(93)90087-E","article-title":"The toxicological effect of cyclophosphamide on acetylcholinesterase activity","volume":"66","author":"Al-Jafari","year":"1993","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.chemosphere.2020.127678_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0300-483X(94)02848-O","article-title":"Inhibition of human acetylcholinesterase by cyclophosphamide","volume":"96","author":"Al-Jafari","year":"1995","journal-title":"Toxicology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib5","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.talanta.2014.12.035","article-title":"Multi-residue method for the analysis of pharmaceuticals and some of their metabolites in bivalves","volume":"136","author":"Alvarez-Mu\u00f1oz","year":"2015","journal-title":"Talanta"},{"key":"10.1016\/j.chemosphere.2020.127678_bib6","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.envint.2018.07.006","article-title":"Pharmaceuticals and endocrine disruptors in raw and cooked seafood from European market: concentrations and human exposure levels","volume":"119","author":"Alvarez-Mu\u00f1oz","year":"2018","journal-title":"Environ. Int."},{"key":"10.1016\/j.chemosphere.2020.127678_bib7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0027-5107(95)00039-L","article-title":"Cyclophosphamide: review of its mutagenicity for an assessment of potential germ cell risks. Mutat. Res","volume":"330","author":"Anderson","year":"1995","journal-title":"Fundamental and Molecular Mechanisms of Mutagenesis"},{"key":"10.1016\/j.chemosphere.2020.127678_bib8","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.scitotenv.2019.03.133","article-title":"Exposure to TiO 2 nanoparticles induces shifts in the microbiota composition of Mytilus galloprovincialis hemolymph","volume":"670","author":"Auguste","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2020.127678_bib9","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.envint.2011.10.002","article-title":"Anticancer drugs in surface waters: what can we say about the occurrence and environmental significance of cytotoxic, cytostatic and endocrine therapy drugs?","volume":"39","author":"Besse","year":"2012","journal-title":"Environ. Int."},{"key":"10.1016\/j.chemosphere.2020.127678_bib10","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.foodchem.2013.09.045","article-title":"As, Cd, Hg and Pb in four edible shellfish species from breeding and harvesting areas along the eastern Adriatic Coast, Croatia","volume":"146","author":"Bogdanovic","year":"2014","journal-title":"Food Chem."},{"key":"10.1016\/j.chemosphere.2020.127678_bib11","doi-asserted-by":"crossref","first-page":"7242","DOI":"10.1021\/es0609405","article-title":"Occurrence and fate of the cytostatic drugs cyclophosphamide and ifosfamide in wastewater and surface waters","volume":"40","author":"Buerge","year":"2006","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib12","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A\u00a0rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal","volume":"72","author":"Bradford","year":"1976","journal-title":"Biochemist"},{"key":"10.1016\/j.chemosphere.2020.127678_bib13","first-page":"527","article-title":"Cellular glutathione (GSH) and glutathione S-transferase (GST) activity in human ovarian tumor biopsies following exposure to alkylating agents","volume":"24","author":"Britten","year":"1992","journal-title":"Radiat. Oncol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib14","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.ecoenv.2012.07.010","article-title":"Fate of isotopically labeled zinc oxide nanoparticles in sediment and effects on two endobenthic species, the clam Scrobicularia plana and the ragworm Hediste diversicolor","volume":"84","author":"Buffet","year":"2012","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.chemosphere.2020.127678_bib15","doi-asserted-by":"crossref","first-page":"5807","DOI":"10.1016\/j.chroma.2009.06.001","article-title":"Analysis of pharmaceuticals in indirect potable reuse systems using solid-phase extraction and liquid chromatography-tandem mass spectrometry","volume":"1216","author":"Busetti","year":"2009","journal-title":"J.\u00a0Chromatogr. A"},{"key":"10.1016\/j.chemosphere.2020.127678_bib16","first-page":"186","article-title":"The principles of cancer treatment by chemotherapy","volume":"30","author":"Caley","year":"2012","journal-title":"Surgery"},{"key":"10.1016\/j.chemosphere.2020.127678_bib19","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.marenvres.2011.06.005","article-title":"Bivalve molluscs as a unique target group for nanoparticle toxicity","volume":"76","author":"Canesi","year":"2012","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.chemosphere.2020.127678_bib18","first-page":"464","article-title":"Effects of Triclosan on Mytilus galloprovincialis hemocyte function and digestive gland enzyme activities: possible modes of action on non-target organisms. Comp. Biochem. Physiol. - C Toxicol","volume":"145","author":"Canesi","year":"2007","journal-title":"Pharmacology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib20","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.chemosphere.2011.10.040","article-title":"Can Hediste diversicolor (Nereidae, Polychaete) be considered a good candidate in evaluating PAH contamination? A multimarker approach","volume":"86","author":"Catalano","year":"2012","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2020.127678_bib21","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.envpol.2015.12.017","article-title":"Ecotoxicity and genotoxicity of cyclophosphamide, ifosfamide, their metabolites\/transformation products and their mixtures","volume":"210","author":"\u010cesen","year":"2016","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.chemosphere.2020.127678_bib22","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.foodchem.2017.07.072","article-title":"Occurrence of antiobiotics in mussels and clams from various FAO areas","volume":"240","author":"Chiesa","year":"2018","journal-title":"Food Chem."},{"key":"10.1016\/j.chemosphere.2020.127678_bib23","first-page":"167","article-title":"Highly active human pharmaceuticals in aquatic systems: a concept for their identification based on their mode of action. Aquat","volume":"96","author":"Christen","year":"2010","journal-title":"Toxicology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib25","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1002\/biof.5520170112","article-title":"Detoxification of electrophilic compounds by glutathione S -transferase catalysis: determinants of individual response to chemical carcinogens and chemotherapeutic drugs","volume":"17","author":"Coles","year":"2003","journal-title":"Biofactors"},{"key":"10.1016\/j.chemosphere.2020.127678_bib26","first-page":"294","article-title":"Chemotherapy-associated oxidative stress: impact on chemotherapeutic effectiveness. Integr","volume":"3","author":"Conklin","year":"2004","journal-title":"Canc. Ther."},{"key":"10.1016\/j.chemosphere.2020.127678_bib27","doi-asserted-by":"crossref","first-page":"315","DOI":"10.2174\/1570159X11311030006","article-title":"Acetylcholinesterase inhibitors: pharmacology and toxicology","volume":"11","author":"Colovic","year":"2013","journal-title":"Curr. Neuropharmacol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib28","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1006\/eesa.1999.1842","article-title":"Antioxidant biomarkers in freshwater bivalves, Unio tumidus, in response to different contamination profiles of aquatic sediments","volume":"45","author":"Cossu","year":"2000","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.chemosphere.2020.127678_bib29","first-page":"134","article-title":"Principles of cancer treatment by chemotherapy","volume":"36","author":"Dickens","year":"2017","journal-title":"Surgery"},{"key":"10.1016\/j.chemosphere.2020.127678_bib30","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/0378-4274(95)03570-2","article-title":"Glutathione peroxidase and oxidative stress","volume":"82\u201383","author":"Doroshow","year":"1995","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.chemosphere.2020.127678_bib31","first-page":"19","article-title":"Season influences the transcriptomic effects of dietary exposure to PVP\/PEI coated Ag nanoparticles on mussels Mytilus galloprovincialis. Comp. Biochem. Physiol. Part - C Toxicol","volume":"222","author":"Duroudier","year":"2019","journal-title":"Pharmacology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib118","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A\u00a0new and rapid colorimetric determination of acetylcholinesterase activity","volume":"7","author":"Ellman","year":"1961","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib32","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1038\/nrclinonc.2009.146","article-title":"Cyclophosphamide and cancer: golden anniversary","volume":"6","author":"Emadi","year":"2009","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib33","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1021\/tx970180z","article-title":"Reactions of N-Methyl-2-phenylindole with malondialdehyde and 4-hydroxyalkenals. Mechanistic aspects of the colorimetric assay of lipid peroxidation","volume":"11","author":"Erdelmeier","year":"1998","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib34","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.scitotenv.2018.04.264","article-title":"Mussel digestive gland as a model tissue for assessing xenobiotics: an overview","volume":"636","author":"Faggio","year":"2018","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2020.127678_bib35","first-page":"122","article-title":"Ecotoxicology of human pharmaceuticals. Aquat","volume":"76","author":"Fent","year":"2006","journal-title":"Toxicology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib36","doi-asserted-by":"crossref","first-page":"E359","DOI":"10.1002\/ijc.29210","article-title":"Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012","volume":"136","author":"Ferlay","year":"2015","journal-title":"Int. J. Canc."},{"key":"10.1016\/j.chemosphere.2020.127678_bib38","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.envpol.2014.07.002","article-title":"Incidence of anticancer drugs in an aquatic urban system: from hospital effluents through urban wastewater to natural environment. Environ","volume":"193","author":"Ferrando-Climent","year":"2014","journal-title":"Pollut"},{"key":"10.1016\/j.chemosphere.2020.127678_bib39","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.scitotenv.2016.09.185","article-title":"Ecotoxicological assessment of the anticancer drug cisplatin in the polychaete Nereis diversicolor","volume":"575","author":"Fonseca","year":"2017","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2020.127678_bib40","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.envint.2019.05.014","article-title":"Impacts of in\u00a0vivo and in\u00a0vitro exposures to tamoxifen: comparative effects on human cells and marine organisms","volume":"129","author":"Fonseca","year":"2019","journal-title":"Environ. Int."},{"key":"10.1016\/j.chemosphere.2020.127678_bib41","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1016\/j.scitotenv.2018.04.318","article-title":"Environmental relevant levels of the cytotoxic drug cyclophosphamide produce harmful effects in the polychaete Nereis diversicolor","volume":"636","author":"Fonseca","year":"2018","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2020.127678_bib42","doi-asserted-by":"crossref","first-page":"e105028","DOI":"10.1371\/journal.pone.0105028","article-title":"Are phar- maceuticals with evolutionary conserved molecular drug targets more potent to cause toxic effects in non-target organisms?","volume":"9","author":"Furuhagen","year":"2014","journal-title":"PloS One"},{"key":"10.1016\/j.chemosphere.2020.127678_bib44","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.freeradbiomed.2017.01.004","article-title":"Reactive oxygen species and cancer paradox: to promote or to suppress?","volume":"104","author":"Galadari","year":"2017","journal-title":"Free Radic. Biol. Med. J"},{"key":"10.1016\/j.chemosphere.2020.127678_bib45","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.bbrc.2016.10.086","article-title":"Lipid peroxidation in cell death","volume":"482","author":"Gaschler","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.chemosphere.2020.127678_bib119","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1023\/A:1026108306755","article-title":"Antioxidant enzyme activities, metallothioneins and lipid peroxidation as biomarkers in Ruditapes decussatus?","volume":"12","author":"Geret","year":"2003","journal-title":"Ecotoxicology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib46","doi-asserted-by":"crossref","first-page":"3986","DOI":"10.1021\/jm00049a018","article-title":"Chemical and biological evaluation of hydrolysis products of cyclophosphamide","volume":"37","author":"Gilard","year":"1994","journal-title":"J.\u00a0Med. Chem."},{"key":"10.1016\/j.chemosphere.2020.127678_bib47","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.marenvres.2012.11.009","article-title":"Genotoxicity of copper oxide and silver nanoparticles in the mussel Mytilus galloprovincialis","volume":"84","author":"Gomes","year":"2013","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.chemosphere.2020.127678_bib48","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/S0887-2333(03)00093-6","article-title":"Comparative effects of cadmium, copper, paraquat and benzo [a] pyrene on the actin cytoskeleton and production of reactive oxygen species (ROS) in mussel hemocytes","volume":"17","author":"G\u00f3mez-Mendikute","year":"2003","journal-title":"Toxicol. Vitro"},{"key":"10.1016\/j.chemosphere.2020.127678_bib49","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.etap.2011.12.017","article-title":"Does non-steroidal anti-inflammatory (NSAID) ibuprofen induce antioxidant stress and endocrine disruption in mussel Mytilus galloprovincialis?","volume":"33","author":"Gonzalez-Rey","year":"2012","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib50","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.envpol.2012.10.018","article-title":"Does selective serotonin reuptake inhibitor (SSRI) fluoxetine affects mussel Mytilus galloprovincialis?","volume":"173","author":"Gonzalez-Rey","year":"2013","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.chemosphere.2020.127678_bib51","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.aquatox.2014.02.006","article-title":"Effects of active pharmaceutical ingredients mixtures in mussel Mytilus galloprovincialis","volume":"153","author":"Gonzalez-Rey","year":"2014","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib52","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.aquatox.2014.01.011","article-title":"Effects of non-steroidal anti-inflammatory drug (NSAID) diclofenac exposure in mussel Mytilus galloprovincialis","volume":"148","author":"Gonzalez-Rey","year":"2014","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib53","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1038\/nrd4002","article-title":"Modulation of oxidative stress as an anticancer strategy","volume":"12","author":"Gorrini","year":"2013","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.chemosphere.2020.127678_bib54","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0163-7258(91)90083-X","article-title":"Drug metabolism in carcinogenesis and cancer chemotherapy","volume":"51","author":"Graham","year":"1991","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.chemosphere.2020.127678_bib55","doi-asserted-by":"crossref","first-page":"7130","DOI":"10.1016\/S0021-9258(19)42083-8","article-title":"Glutathione S-transferase: the first enzymatic step in mercapturic acid formation","volume":"249","author":"Habig","year":"1974","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chemosphere.2020.127678_bib56","first-page":"3016","article-title":"Comparison of the mutagenicity and teratogenicity of cyclophosphamide and its active metabolites, 4-hydroxycyclophosphamide, phosphoramide mustard, and acrolein","volume":"42","author":"Hales","year":"1982","journal-title":"Canc. Res."},{"key":"10.1016\/j.chemosphere.2020.127678_bib58","doi-asserted-by":"crossref","first-page":"14687","DOI":"10.1007\/s11356-016-7069-3","article-title":"Fate and effects of the residues of anticancer drugs in the environment","volume":"23","author":"Heath","year":"2016","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.chemosphere.2020.127678_bib59","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.marpolbul.2013.12.045","article-title":"Emerging organic contaminants in coastal waters: anthropogenic impact, environmental release and ecological risk","volume":"85","author":"Jiang","year":"2014","journal-title":"Mar. Pollut. Bull."},{"key":"10.1016\/j.chemosphere.2020.127678_bib60","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.jhydrol.2007.09.054","article-title":"Do cytotoxic chemotherapy drugs discharged into rivers pose a risk to the environment and human health? An overview and UK case study","volume":"348","author":"Johnson","year":"2008","journal-title":"J.\u00a0Hydrol"},{"key":"10.1016\/j.chemosphere.2020.127678_bib61","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1002\/etc.2311","article-title":"Predicting concentrations of the cytostatic drugs cyclophosphamide, carboplatin, 5-fluorouracil, and capecitabine throughout the sewage effluents and surface waters of Europe","volume":"32","author":"Johnson","year":"2013","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2020.127678_bib62","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.fct.2014.05.012","article-title":"Human exposure to trace metals and possible public health risks via consumption of mussels Mytilus galloprovincialis from de Adriatic coastal area","volume":"70","author":"Jovic","year":"2014","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib63","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.aquatox.2014.02.003","article-title":"Cytotoxicity and cellular mechanisms involved in the toxicity of CdS quantum dots in hemocytes and gill cells of the mussel Mytilus galloprovincialis","volume":"153","author":"Katsumiti","year":"2014","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib64","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.tiv.2018.01.013","article-title":"Cytotoxicity and cellular mechanisms of toxicity of CuO NPs in mussel cells in\u00a0vitro and comparative sensitivity with human cells","volume":"48","author":"Katsumiti","year":"2018","journal-title":"Toxicol. Vitro"},{"key":"10.1016\/j.chemosphere.2020.127678_bib65","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1016\/j.scitotenv.2019.03.187","article-title":"In\u00a0vitro toxicity testing in hemocytes of the marine mussel Mytilus galloprovincialis (L.) to uncover mechanisms of action of the water accommodated fraction (WAF) of a naphthenic North Sea crude oil without and with dispersant","volume":"670","author":"Katsumiti","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2020.127678_bib66","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.aquatox.2017.04.016","article-title":"Intracellular localization and toxicity of graphene oxide and reduced graphene oxide nanoplatelets to mussel hemocytes in\u00a0vitro","volume":"188","author":"Katsumiti","year":"2017","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib67","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1097\/FPC.0b013e3283656bc1","article-title":"PharmGKB summary: tamoxifen pathway, pharmacokinetics","volume":"23","author":"Klein","year":"2013","journal-title":"Pharmacogenetics Genom."},{"key":"10.1016\/j.chemosphere.2020.127678_bib68","first-page":"1065","volume":"30","author":"Kosjek","year":"2011"},{"key":"10.1016\/j.chemosphere.2020.127678_bib69","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1146\/annurev-environ-052809-161223","article-title":"Pharmaceuticals in the environment","volume":"35","author":"K\u00fcmmerer","year":"2010","journal-title":"Annu. Rev. Environ. Resour."},{"key":"10.1016\/j.chemosphere.2020.127678_bib120","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1016\/0006-291X(76)90747-6","article-title":"Glutathione peroxidase activity in selenium-deficient rat liver","volume":"71","author":"Lawrence","year":"1976","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.chemosphere.2020.127678_bib70","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.scitotenv.2004.11.007","article-title":"Presence of cancerostatic platinum compounds in hospital wastewater and possible elimination by adsorption to activated sludge","volume":"345","author":"Lenz","year":"2005","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2020.127678_bib71","series-title":"Microplastic Contamination in Aquatic Environments","first-page":"133","article-title":"The occurrence, fate, and effects of microplastics in the marine environment","author":"Li","year":"2018"},{"key":"10.1016\/j.chemosphere.2020.127678_bib72","doi-asserted-by":"crossref","first-page":"479","DOI":"10.3109\/10715761003667554","article-title":"Reactive oxygen species in cancer","volume":"44","author":"Liou","year":"2010","journal-title":"Free Radic. Res."},{"key":"10.1016\/j.chemosphere.2020.127678_bib74","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.aquatox.2009.06.015","article-title":"Effects of environmental concentrations of the antiepilectic drug carbamazepine on biomarkers and cAMP-mediated cell signaling in the mussel Mytilus galloprovincialis","volume":"94","author":"Martin-Diaz","year":"2009","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.chemosphere.2020.127678_bib76","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.envint.2013.11.011","article-title":"In\u00a0vitro tests aiding ecological risk assessment of ciprofloxacin, tamoxifen and cyclophosphamide in range of concentrations released in hospital wastewater and surface water","volume":"63","author":"Mater","year":"2014","journal-title":"Environ. Int."},{"key":"10.1016\/j.chemosphere.2020.127678_bib77","doi-asserted-by":"crossref","first-page":"14302","DOI":"10.1038\/s41598-019-50607-0","article-title":"Ecotoxicological hazard of a mixture of glyphosate and aminomethylphosphonic acid to the mussel Mytilus galloprovincialis (Lamarck 1819)","volume":"9","author":"Matozzo","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.chemosphere.2020.127678_bib80","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1016\/j.chemosphere.2006.02.003","article-title":"Occurrences of pharmaceutical and personal care products as micropollutants in rivers from Romania","volume":"64","author":"Moldovan","year":"2006","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2020.127678_bib81","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.marenvres.2016.11.003","article-title":"Effects of novobiocin and methotrexate on the benthic amphipod Ampelisca brevicornis exposed to spiked sediments","volume":"122","author":"Moreira","year":"2016","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.chemosphere.2020.127678_bib82","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0378-4274(03)00216-9","article-title":"Effects of histone deacetylation and DNA methylation on the constitutive and TCDD-inducible expressions of the human CYP1 family in MCF-7 and HeLa cells","volume":"144","author":"Nakajima","year":"2003","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.chemosphere.2020.127678_bib84","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1016\/j.febslet.2006.01.092","article-title":"Cyclophosphamide enhances TNF-\u03b1-induced apoptotic cell death in murine vascular endothelial cell","volume":"580","author":"Ohtani","year":"2006","journal-title":"FEBS Lett."},{"key":"10.1016\/j.chemosphere.2020.127678_bib85","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.watres.2017.03.052","article-title":"Physiological and biochemical alterations induced in the mussel after short and long-term exposure to carbamazepine Mytilus","volume":"117","author":"Oliveira","year":"2017","journal-title":"Water Res."},{"key":"10.1016\/j.chemosphere.2020.127678_bib86","doi-asserted-by":"crossref","first-page":"213","DOI":"10.4155\/fmc.12.197","article-title":"Cytochrome P450-activated prodrugs","volume":"5","author":"Ortiz de Montellano","year":"2013","journal-title":"Future Med. Chem."},{"key":"10.1016\/j.chemosphere.2020.127678_bib87","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1016\/S0024-3205(01)00969-9","article-title":"The role of oxidative stress in developmental and reproductive toxicity of tamoxifen","volume":"68","author":"Pagano","year":"2001","journal-title":"Life Sci."},{"key":"10.1016\/j.chemosphere.2020.127678_bib89","first-page":"3846","article-title":"In - vitro cytotoxicity activity of solanum nigrum extract against hela cell line and vero cell line","volume":"1","author":"Patel","year":"2009","journal-title":"Int. J. Pharm. Pharmaceut. Sci."},{"key":"10.1016\/j.chemosphere.2020.127678_bib90","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.biopha.2018.02.068","article-title":"Crocin attenuates cyclophosphamide induced testicular toxicity by preserving glutathione redox system","volume":"101","author":"Potnuri","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.chemosphere.2020.127678_bib91","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.abb.2004.02.002","article-title":"Characterization of a class alpha glutathione-S-transferase with glutathione peroxidase activity in human liver microsomes","volume":"424","author":"Prabhu","year":"2004","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.chemosphere.2020.127678_bib93","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1038\/nprot.2008.75","article-title":"Neutral red uptake assay for the estimation of cell viability\/cytotoxicity","volume":"3","author":"Repetto","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.chemosphere.2020.127678_bib98","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/S0090-9556(24)15302-0","article-title":"Development of a substrate-activity based approach to identify the major human liver P-450 catalysts of cyclophosphamide and ifosfamide activation based on cDNA-expressed activities and liver microsomal P-450 profiles. Drug Metab","volume":"27","author":"Roy","year":"1999","journal-title":"Dispos"},{"key":"10.1016\/j.chemosphere.2020.127678_bib100","doi-asserted-by":"crossref","first-page":"2733","DOI":"10.1897\/09-067.1","article-title":"Cytotoxic drugs in drinking water: a prediction and risk assessment exercise for the Thames catchment in the United Kingdom","volume":"28","author":"Rowney","year":"2009","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2020.127678_bib101","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.tox.2005.08.020","article-title":"Chemoprotective effect of lipoic acid against cyclophosphamide-induced changes in the rat sperm","volume":"217","author":"Selvakumar","year":"2006","journal-title":"Toxicology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib102","doi-asserted-by":"crossref","first-page":"845","DOI":"10.2174\/187152711798072365","article-title":"Interaction of human brain acetylcholinesterase with cyclophosphamide: a molecular modeling and docking study. CNS. Neurol. Disord","volume":"10","author":"Shakil","year":"2011","journal-title":"Drug Targets"},{"key":"10.1016\/j.chemosphere.2020.127678_bib103","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.scitotenv.2017.06.076","article-title":"SSRIs antidepressants in marine mussels from Atlantic coastal areas and human risk assessment","volume":"603\u2013604","author":"Silva","year":"2017","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2020.127678_bib104","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.ecoenv.2017.12.038","article-title":"Experimental exposure of blue mussels (Mytilus galloprovincialis) to high levels of benzo[a]pyrene and possible implications for human health","volume":"150","author":"Speciale","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.chemosphere.2020.127678_bib105","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1515\/DMDI.2002.19.2.67","article-title":"Effects of amifostine on liver oxidative stress caused by cyclophosphamide","volume":"19","author":"Stankiewicz","year":"2002","journal-title":"Drug Metabol. Drug Interact."},{"key":"10.1016\/j.chemosphere.2020.127678_bib106","first-page":"2","article-title":"Trypan blue exclusion test of cell viability","author":"Strober","year":"2001","journal-title":"Curr. Protoc. Im."},{"key":"10.1016\/j.chemosphere.2020.127678_bib107","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.toxlet.2011.08.022","article-title":"Biomarkers of occupational exposure do anticancer agents: a minireview","volume":"207","author":"Suspiro","year":"2011","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.chemosphere.2020.127678_bib108","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.mrrev.2014.02.001","article-title":"Environmental risk assessment of anti-cancer drugs and their transformation products: a focus on their genotoxicity characterization-state of knowledge and short comings","volume":"760","author":"Toolaram","year":"2014","journal-title":"Mutat. Res."},{"key":"10.1016\/j.chemosphere.2020.127678_bib109","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.cbi.2009.03.017","article-title":"Intervention of astaxanthin against cyclophosphamide-induced oxidative stress and DNA damage: a study in mice","volume":"180","author":"Tripathi","year":"2009","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.chemosphere.2020.127678_bib110","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.marenvres.2016.05.004","article-title":"Toxic effects of cisplatin cytostatic drug in mussel Mytilus galloprovincialis","volume":"119","author":"Trombini","year":"2016","journal-title":"Mar. Environ. Res."},{"key":"10.1016\/j.chemosphere.2020.127678_bib111","first-page":"57","article-title":"Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ. Toxicol","volume":"13","author":"Van der Oost","year":"2003","journal-title":"Pharmacology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib113","first-page":"1685","article-title":"Activation of the anti-cancer drug ifosphamide by rat liver microsomal P450 enzymes. Biochem","volume":"45","author":"Weber","year":"1993","journal-title":"Pharmacology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib114","series-title":"Global Status Report on Non-communicable Diseases","first-page":"302","year":"2014"},{"key":"10.1016\/j.chemosphere.2020.127678_bib115","first-page":"137","article-title":"Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat","volume":"19","author":"Winston","year":"1991","journal-title":"Toxicology"},{"key":"10.1016\/j.chemosphere.2020.127678_bib116","doi-asserted-by":"crossref","first-page":"26141","DOI":"10.1074\/jbc.274.37.26141","article-title":"Human endothelial cell life extension by telomerase expression","volume":"274","author":"Yang","year":"1999","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.chemosphere.2020.127678_bib117","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.scitotenv.2012.12.061","article-title":"Removal of cytostatic drugs from aquatic environment: a review","volume":"445\u2013446","author":"Zhang","year":"2013","journal-title":"Sci. Total Environ."}],"container-title":["Chemosphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653520318737?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653520318737?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T11:24:33Z","timestamp":1758626673000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0045653520318737"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":102,"alternative-id":["S0045653520318737"],"URL":"https:\/\/doi.org\/10.1016\/j.chemosphere.2020.127678","relation":{},"ISSN":["0045-6535"],"issn-type":[{"value":"0045-6535","type":"print"}],"subject":[],"published":{"date-parts":[[2020,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cytotoxic responses of the anticancer drug cyclophosphamide in the mussel Mytilus galloprovincialis and comparative sensitivity with human cells lines","name":"articletitle","label":"Article Title"},{"value":"Chemosphere","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chemosphere.2020.127678","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"127678"}}