{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T01:00:34Z","timestamp":1793581234588,"version":"4.5.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100010014","name":"Six Talent Peaks Project in Jiangsu Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemosphere"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1016\/j.chemosphere.2020.129324","type":"journal-article","created":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T02:53:40Z","timestamp":1608087220000},"page":"129324","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":37,"special_numbering":"C","title":["Energy-saving preparation of a bioflocculant under high-salt condition by using strain Bacillus sp. and the interaction mechanism towards heavy metals"],"prefix":"10.1016","volume":"267","author":[{"given":"Jing-qiu","family":"Hua","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong-ping","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4742-6194","authenticated-orcid":false,"given":"Guang-xiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3975-8903","authenticated-orcid":false,"given":"Lei","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chemosphere.2020.129324_bib1","doi-asserted-by":"crossref","first-page":"2689","DOI":"10.3390\/molecules23102689","article-title":"Production and characterization of a bioflocculant produced by Bacillus salmalaya 139SI-7 and its applications in wastewater treatment","volume":"23","author":"Abu Tawila","year":"2018","journal-title":"Molecules"},{"key":"10.1016\/j.chemosphere.2020.129324_bib2","doi-asserted-by":"crossref","first-page":"27825","DOI":"10.1039\/C9RA04683F","article-title":"A\u00a0novel efficient bioflocculant QZ-7 for the removal of heavy metals from industrial wastewater","volume":"9","author":"Abu Tawila","year":"2019","journal-title":"RSC Adv."},{"key":"10.1016\/j.chemosphere.2020.129324_bib3","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1080\/02757540.2019.1584614","article-title":"Equilibrium, kinetics and thermodynamics of hexavalent chromium biosorption on pristine and zinc chloride activated Senna siamea seed pods","volume":"35","author":"Ajmani","year":"2019","journal-title":"Chem. Ecol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib4","first-page":"466","article-title":"Flocculation behavior and mechanism of bioflocculant produced by Aspergillus flavus","volume":"150","author":"Aljuboori","year":"2015","journal-title":"J.\u00a0Environ. Manag."},{"key":"10.1016\/j.chemosphere.2020.129324_bib5","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.chemosphere.2019.05.092","article-title":"Bioflocculant production and heavy metal sorption by metal resistant bacterial isolates from gold mining soil","volume":"231","author":"Ayangbenro","year":"2019","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2020.129324_bib6","doi-asserted-by":"crossref","first-page":"9169","DOI":"10.1007\/s00253-019-10188-4","article-title":"Role of extracellular polymeric substances in biofilm formation by Pseudomonas stutzeri strain XL-2","volume":"103","author":"Ding","year":"2019","journal-title":"Appl. Microbiol. Biotechonol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib7","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.3390\/ijerph16122185","article-title":"Optimization and application of bioflocculant passivated copper nanoparticles in the wastewater treatment","volume":"16","author":"Dlamini","year":"2019","journal-title":"Int. J. Environ. Res. Publ. Health"},{"key":"10.1016\/j.chemosphere.2020.129324_bib8","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.biortech.2016.11.040","article-title":"Studies of cadmium(II), lead(II), nickel(II), cobalt(II) and chromium(VI) sorption on extracellular polymeric substances produced by Rhodococcus opacus and Rhodococcus rhodochrous","volume":"225","author":"Dobrowolski","year":"2017","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib9","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.jhazmat.2018.09.063","article-title":"Preparation of a bioflocculant by using acetonitrile as sole nitrogen source and its application in heavy metals removal","volume":"363","author":"Fan","year":"2019","journal-title":"J.\u00a0Hazard Mater."},{"key":"10.1016\/j.chemosphere.2020.129324_bib10","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.biortech.2013.09.011","article-title":"The adsorption behavior and mechanism investigation of Pb(II) removal by flocculation using microbial flocculant GA1","volume":"148","author":"Feng","year":"2013","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib11","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.ecoenv.2015.06.010","article-title":"Production and characterization of a thermostable bioflocculant from Bacillus subtilis F9, isolated from wastewater sludge","volume":"121","author":"Giri","year":"2015","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.chemosphere.2020.129324_bib12","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.chemosphere.2017.04.119","article-title":"Removal of arsenite by a microbial bioflocculant produced from swine wastewater","volume":"181","author":"Guo","year":"2017","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2020.129324_bib13","doi-asserted-by":"crossref","first-page":"S306","DOI":"10.1002\/ep.13027","article-title":"Multiple pollutants removal from industrial wastewaters using a novel bioflocculant produced by Bacillus licheniformis NJ3","volume":"38","author":"Joshi","year":"2019","journal-title":"Environ. Prog. Sustain. Energy"},{"key":"10.1016\/j.chemosphere.2020.129324_bib14","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.biortech.2016.11.050","article-title":"A\u00a0novel method to harvest Chlorella sp. via low cost bioflocculant: influence of temperature with kinetic and thermodynamic functions","volume":"225","author":"Kothari","year":"2017","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib15","doi-asserted-by":"crossref","DOI":"10.1007\/s10661-020-8181-x","article-title":"Kinetic and thermodynamic studies on biosorption of Cr(VI) on raw and chemically modified Datura stramonium fruit","volume":"192","author":"Kumar","year":"2020","journal-title":"Environ. Monit. Assess."},{"key":"10.1016\/j.chemosphere.2020.129324_bib16","doi-asserted-by":"crossref","first-page":"22808","DOI":"10.1021\/acsami.7b02908","article-title":"Self-assembled exopolysaccharide nanoparticles for bioremediation and green synthesis of noble metal nanoparticles","volume":"9","author":"Li","year":"2017","journal-title":"Acs Appl. Mater. Inter."},{"key":"10.1016\/j.chemosphere.2020.129324_bib17","article-title":"Optimization of fermentation conditions and properties of an exopolysaccharide from Klebsiella sp H-207 and application in adsorption of hexavalent chromium","volume":"8","author":"Li","year":"2013","journal-title":"PloS One"},{"key":"10.1016\/j.chemosphere.2020.129324_bib18","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/s13068-017-0780-6","article-title":"Valorization of untreated rice bran towards bioflocculant using a lignocellulose-degrading strain and its use in microalgal biomass harvest","volume":"10","author":"Liu","year":"2017","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.chemosphere.2020.129324_bib19","doi-asserted-by":"crossref","first-page":"121505","DOI":"10.1016\/j.biortech.2019.121505","article-title":"Bioconversion of kitchen wastes into bioflocculant and its pilot-scale application in treating iron mineral processing wastewater","volume":"288","author":"Liu","year":"2019","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib20","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1186\/s13068-015-0354-4","article-title":"Bioflocculant production from untreated corn stover using Cellulosimicrobium cellulans L804 isolate and its application to harvesting microalgae","volume":"8","author":"Liu","year":"2015","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.chemosphere.2020.129324_bib21","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1080\/09593330.2017.1377770","article-title":"Production of a bioflocculant from Klebsiella sp. OS-1 using brewery wastewater as a source","volume":"40","author":"Ma","year":"2019","journal-title":"Environ. Technol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib22","doi-asserted-by":"crossref","first-page":"4001","DOI":"10.3390\/ijerph16204001","article-title":"Removal of pollutants in mine wastewater by a non-cytotoxic polymeric bioflocculant from Alcaligenes faecalis HCB2","volume":"16","author":"Maliehe","year":"2019","journal-title":"Int. J. Environ. Res. Publ. Health"},{"key":"10.1016\/j.chemosphere.2020.129324_bib23","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s11274-019-2696-8","article-title":"Culture optimization for production and characterization of bioflocculant by Aspergillus flavus grown on chicken viscera hydrolysate","volume":"35","author":"Mohammed","year":"2019","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib24","doi-asserted-by":"crossref","first-page":"66037","DOI":"10.1039\/C5RA08636A","article-title":"Production of a non-cytotoxic bioflocculant by a bacterium utilizing a petroleum hydrocarbon source and its application in heavy metal removal","volume":"5","author":"Pathak","year":"2015","journal-title":"RSC Adv."},{"key":"10.1016\/j.chemosphere.2020.129324_bib25","doi-asserted-by":"crossref","first-page":"170","DOI":"10.3389\/fmicb.2017.00170","article-title":"Characterization of a novel polymeric bioflocculant produced from bacterial utilization of n-Hexadecane and its application in removal of heavy metals","volume":"8","author":"Pathak","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib26","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0190236","article-title":"Isolation, identification, and characterization of an Aspergillus Niger bioflocculant-producing strain using potato starch wastewater as nutrilite and its application","volume":"13","author":"Pu","year":"2018","journal-title":"PloS One"},{"key":"10.1016\/j.chemosphere.2020.129324_bib27","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.1007\/s00253-019-09643-z","article-title":"Trichosporon fermentans biomass flocculation from soybean oil refinery wastewater using bioflocculant produced from Paecilomyces sp. M2-1","volume":"103","author":"Qiao","year":"2019","journal-title":"Appl. Microbiol. Biotechonol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib28","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.ijbiomac.2020.02.181","article-title":"Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture","volume":"151","author":"Shahnaz","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib29","article-title":"Multivariate optimisation of Cr (VI), Co (III) and Cu (II) adsorption onto nanobentonite incorporated nanocellulose\/chitosan aerogel using response surface methodology","volume":"36","author":"Shahnaz","year":"2020","journal-title":"J.\u00a0Water Process Eng."},{"key":"10.1016\/j.chemosphere.2020.129324_bib30","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.watres.2012.10.037","article-title":"Thermodynamic analysis on the binding of heavy metals onto extracellular polymeric substances (EPS) of activated sludge","volume":"47","author":"Sheng","year":"2013","journal-title":"Water Res."},{"key":"10.1016\/j.chemosphere.2020.129324_bib31","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s00253-004-1704-5","article-title":"Extraction of extracellular polymeric substances from the photosynthetic bacterium Rhodopseudomonas acidophila","volume":"67","author":"Sheng","year":"2005","journal-title":"Appl. Microbiol. Biotechonol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib32","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1021\/acs.energyfuels.8b03513","article-title":"Stoichiometry and thermodynamic analysis on biohydrogen production from xylose by Klebsiella oxytoca GS-4-08","volume":"33","author":"Song","year":"2019","journal-title":"Energy Fuel."},{"key":"10.1016\/j.chemosphere.2020.129324_bib33","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.biortech.2017.06.103","article-title":"Uncovering the flocculating potential of extracellular polymeric substances produced by periphytic biofilms","volume":"248","author":"Sun","year":"2018","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib34","doi-asserted-by":"crossref","first-page":"109052","DOI":"10.1016\/j.envres.2019.109052","article-title":"Cultivation substrata differentiate the properties of river biofilm EPS and their binding of heavy metals: a spectroscopic insight","volume":"182","author":"Wang","year":"2020","journal-title":"Environ. Res."},{"key":"10.1016\/j.chemosphere.2020.129324_bib35","doi-asserted-by":"crossref","first-page":"2559","DOI":"10.1007\/s11274-011-0726-2","article-title":"Characterization of a compound bioflocculant produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6","volume":"27","author":"Wang","year":"2011","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib36","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.biortech.2012.01.149","article-title":"Fluorescence-based rapid assessment of the biological stability of landfilled municipal solid waste","volume":"110","author":"Wu","year":"2012","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib37","doi-asserted-by":"crossref","first-page":"136959","DOI":"10.1016\/j.scitotenv.2020.136959","article-title":"Adsorption of cyanobacterial extracellular polymeric substance on colloidal particle: influence of molecular weight","volume":"715","author":"Xu","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2020.129324_bib38","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.scitotenv.2018.03.327","article-title":"Enterobacter aerogenes metabolites enhance Microcystis aeruginosa biomass recovery for sustainable bioflocculant and biohydrogen production","volume":"634","author":"Xu","year":"2018","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2020.129324_bib39","doi-asserted-by":"crossref","first-page":"121697","DOI":"10.1016\/j.jhazmat.2019.121697","article-title":"Simultaneous nitriles degradation and bioflocculant production by immobilized K.\u00a0oxytoca strain in a continuous flow reactor","volume":"387","author":"Yu","year":"2020","journal-title":"J.\u00a0Hazard Mater."},{"key":"10.1016\/j.chemosphere.2020.129324_bib40","doi-asserted-by":"crossref","first-page":"34980","DOI":"10.1038\/srep34980","article-title":"A\u00a0novel Fe(III) dependent bioflocculant from Klebsiella oxytoca GS-4-08: culture conditions optimization and flocculation mechanism","volume":"6","author":"Yu","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.chemosphere.2020.129324_bib41","doi-asserted-by":"crossref","first-page":"121535","DOI":"10.1016\/j.biortech.2019.121535","article-title":"Enhanced decolorization of methyl orange by Bacillus sp. strain with magnetic humic acid nanoparticles under high salt conditions","volume":"288","author":"Yu","year":"2019","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib42","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1021\/es1030905","article-title":"Identification of key constituents and structure of the extracellular polymeric substances excreted by Bacillus megaterium TF10 for their flocculation capacity","volume":"45","author":"Yuan","year":"2011","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.chemosphere.2020.129324_bib43","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1007\/s00253-002-1023-7","article-title":"Characterization of a bioflocculant produced by the marine myxobacterium Nannocystis sp NU-2","volume":"59","author":"Zhang","year":"2002","journal-title":"Appl. Microbiol. Biotechonol."}],"container-title":["Chemosphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653520335219?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653520335219?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T11:41:18Z","timestamp":1758627678000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0045653520335219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":43,"alternative-id":["S0045653520335219"],"URL":"https:\/\/doi.org\/10.1016\/j.chemosphere.2020.129324","relation":{},"ISSN":["0045-6535"],"issn-type":[{"value":"0045-6535","type":"print"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Energy-saving preparation of a bioflocculant under high-salt condition by using strain Bacillus sp. and the interaction mechanism towards heavy metals","name":"articletitle","label":"Article Title"},{"value":"Chemosphere","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chemosphere.2020.129324","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"129324"}}